• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.785-792
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• 2020

ʟ-Theanine, found in green tea leaves has been shown to positively affect immunity and relaxation in humans. There have been many attempts to produce ʟ-theanine through enzymatic synthesis to overcome the limitations of traditional methods. Among the many genes coding for enzymes in the ʟ-theanine biosynthesis, glutamylmethylamide synthetase (GMAS) exhibits the greatest possibility of producing large amounts of production. Thus, GMAS from Methylovorus mays No. 9 was overexpressed in several strains including vectors with different copy numbers. BW25113(DE3) cells containing the pET24ma::gmas was selected for strains. The optimal temperature, pH, and metal ion concentration were 50℃, 7, and 5 mM MnCl₂, respectively. Additionally, ATP was found to be an important factor for producing high concentration of ʟ-theanine so several strains were tested during the reaction for ATP regeneration. Baker's yeast was found to decrease the demand for ATP most effectively. Addition of potassium phosphate source was demonstrated by producing 4-fold higher ʟ-theanine. To enhance the conversion yield, GMAS was additionally overexpressed in the system. A maximum of 198 mM ʟ-theanine was produced with 16.5 mmol/l/h productivity. The whole-cell reaction involving GMAS has greatest potential for scale-up production of ʟ-theanine.

• Journal of Welding and Joining
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• v.16 no.4
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• pp.63-72
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• 1998

In this study, the effects of the current pulsing conditions, on the spatter generation rate during the $CO_2$ gas metal arc welding (GMAW) were investigated. Normally using the inverter type power supply, of which the welding current waveform was regulated to reduce the spatter generation rate, but in this study pulsing was imposed on the welding current. Observation of the metal transfer phenomena during the pulsed current GMAS indicated that the droplet transfer from the electrode via the short circuit transfer and the repelling transfer mode could be minimized by selecting optimum combinations of pulsing parameters, which include base and peak current, base and pak duration. It was also demonstrated in this study that proper combinations of the pulsing parameters led to reduce generation of spatters during GMAW shielded by $CO_2$ gas.

• Journal of Soil and Groundwater Environment
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• v.22 no.5
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• pp.1-12
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• 2017

This study reviewed the groundwater policies of USA as a benchmarking for the purpose of improving groundwater polices, regulations, and plans in Korea. Each state of the USA has its own unique groundwater policies. Recently, severe drought in the western parts of the USA resulted in the launch of the California Statewide Groundwater Elevation Monitoring (CASGEM) program. CASGEM classified a total of 515 alluvial groundwater basins of the California State to four prior groups (high, medium, low, and very low prioritization). In Texas, a total of 101 Groundwater Conservation Districts (GCDs) over the state has controlled groundwater pumping amounts in their own management areas and 16 Groundwater Management Areas (GMAs) over the state have directly managed groundwater aquifer. Direct management for aquifers by GMAs would be the most scientific method for groundwater management, which expected to provide water consumers the more advavnced groundwater service. These groundwater management strategies of the USA can be possibly considered in groundwater plans for national and local governmental authorities, which possibly results in more optimal groundwater management in Korea.

• Proceedings of the Korean Printing Society Conference
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• 2002.05a
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• pp.10-18
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• 2002

On the process of cross-media color reproduction, a key feature is the use of gamut mapping techniques to adjust the different color gamuts between displays and printers. Even though a number of GMAs have been published, but there are no method satisfactory enough for more exect color reproduction. In this paper, the gamut mapping methods of nearest point clipping(NPC), centroid clipping (SLIN), straight clipping and cusp clipping(CUSP) were tested and analyzed with color difference, and a new gamut mapping algorithm based on variable anchor point method was proposed.

• Polymer(Korea)
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• v.36 no.1
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• pp.65-70
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• 2012

Glycidyl methacrylate (GMA) was used to introduce epoxy groups on the surface of polypropylene (PP) plate, used as a substrate, through plasma-induced graft copolymerization. Emulsion polymerization was applied for graft copolymerization of GMA and was compared with conventional solution polymerization to confirm its effect. Plasma treatment conditions under one atmospheric pressure were fixed as follows; the RF power of 200 W, the treatment time of 30 sec, the Ar gas flow rate of 6 LPM, and the exposure time of treated PP samples in air of 5 min. For graft-copolymerization, GMA concentration, reaction temperature, and reaction time was optimized to maximize the grafting degree of GMA. The maximum grafting degree of GMA was obtained at the condition of 12%-GMA concentration, $90^{\circ}C$ reaction temperature, and 5 hr-reaction time. Analysis results supported that the emulsion polymerization was more effective than the solution polymerization for grafting more GMAs on the surface of PP plate under the same reaction conditions.