• 한국임상약학회지
• /
• 제2권1호
• /
• pp.11-22
• /
• 1992

Thymic extract showed antitumor effect to sarcoma mice with higher dose$(200{\mu}g/mouse/day$ i.p., 4weeks) but not with low dose$(5{\mu}g/mouse/day$ i.p., 6 weeks). Direct cytotoxicities were exhibited against sarcoma 180, L1210 and MOLT-4 by MTT assay. The spleen weight of mice were increased but the number of circulating lymphocytes were not increased after long-term(2 weeks) administration of thymic extract. Evaluating the mitogenesis by MTT assay. $\%$ absorbance of human lymphocytes was not increased by thymic extract. Cell cycle statistics of S phase and $G_2/M$ phase was not increased in the presence of that by PI staining. The formation of rosette was induced, irrespectively of exposure time short-term(l hour) and long-term(2 weeks). The population of mouse blood T-cell to bind Lyt2-antimonoclonal antibody and to $L_2T_4$ were increased after administration of thymic extract$(2-200{\mu}g/mouse/day)$. From the above results, it is suggested that thymic extract exerts antitumor activity by stimulating T cells to differeniate in vivo but not in vitro.

• Journal of applied mathematics & informatics
• /
• 제16권1_2호
• /
• pp.489-496
• /
• 2004

We study the h- stability of nonlinear difference system x(n+1) =f(n, x(n)) and its perturbed system y(n+l) =f(n, y(n))+g(n, y(n), Sy(n)).

• Weed & Turfgrass Science
• /
• 제6권4호
• /
• pp.322-332
• /
• 2017

골프장 잔디 관리에서 미생물제제는 잔디의 생육 및 품질을 개선하고, 병을 예방하기 위해 사용되어왔다. 본 연구는 유산균(Lactobacillus fermentum)을 함유하는 미생물제제의 처리에 따른 크리핑 벤트그래스의 생육과 품질에 미치는 영향을 조사하였다. 처리구는 무처리구(NF), 대조구(CF), 미생물제제 처리구(MFcL: microbial fertilizer containing L. fermentum; MFL: CF+MFcL $1.0g\;m^{-2}$; 2MFL: CF+ MFcL $2.0g\;m^{-2}$) 및 미생물제제 단독 처리구(OMF; MFcL $1.0g\;m^2$)로 설정하였다. 시험 종료 후 토양 화학성은 처리구별 차이를 나타내지 않아 MFcL의 처리가 토양 이화학성에 미치는 영향은 미미하였다. MFcL 처리 시 잔디의 시각적 품질, 잔디 예지물, 잔디 중 무기 성분 함량 및 흡수량은 대조구와 비슷하였다. 잔디 밀도는 MFcL처리구에서 대조구에 비해 약 20% 정도 증가하였다. 이들 결과는 유산균을 함유하는 미생물제제의 처리는 크리핑 벤트그래스의 밀도를 향상에 기여하는 것을 확인하였다.

• 한국균학회지
• /
• 제6권2호
• /
• pp.11-14
• /
• 1978

Attempts were made to investigate the sterol components of Boletus aerus Bull. which grows wildly in Korea. Its carpophores were collected in Gyeong-Gi Province and extracted with chloroform and methanol. A sterol was isolated and identified as ergosterol by T.L.C., G.L.C. and chemical tests.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.273-276
• /
• 2000

Marine fungus, Thraustochytrium aureum ATCC 34034, was incubated in artificial sea water media in order to produce docosahexaenoic acid(DHA). Cultures were performed at $24^{\circ}C$ in light for 13 days with orbital shaker at 100rpm. Maximum cell productivity of 1.34g/L and DHA yield of 41.4 mg/L were obtained by using this method, which is almost the twice level of DHA yield obtained for the strains reported previously. This strain did produce much more DHA after sporangium disruption.

• 한국식품과학회지
• /
• 제36권2호
• /
• pp.226-232
• /
• 2004

음료 제조를 위한 수박 원료 희석 농도, 당, 구연산, 비타민 C 첨가농도, 살균 방법, 천연 색소 추출 방법, 풍미증진 재료의 첨가량에 따른 색과 맛에 대하여 조사하였다. 주스 제조를 위한 조건을 실험한 결과 수박의 맛과 색이 남아있는 수박즙의 최적 희석 농도는 40%이었고, 음료로서 적정 당도는 $12^{\circ}Brix$였으며, 산미와 항산화 효과를 위한 구연산과 비타민 C첨가 농도는 0.5+0.3 g/L 또는 1.0+0.3 g/L이 적절하였다. 가열살균과 시간을 비교한 결과 수박 음료를 $70^{\circ}C$이상의 온도에서 살균 시 수박 고유의 적색도가 감소되었으며, 관능 검사 결과 $60^{\circ}C$ 10-30분 또는 $70^{\circ}C$에서 10 분간 살균 시 양호하였다 또 $60^{\circ}C$이상으로 살균 시 $30^{\circ}C$에서 한 달 동안 저장 후 처리시간에 관계없이 부패된 것은 발견되지 않았다. 천연 색소 추출 방법에 있어서는 열수 추출에 비해 알콜 추출은 전반적으로 효과가 낮았으며, 첨가 재료에 있어서도 알콜 추출보다는 열수 추출에 유리한 재료인 복분자와 오미자가 효과적이었고 복분자 20 g/L와 오미자 30g/L를 열수추출한 것을 7:3으로 배합하여 음료를 제조했을 때 가장 기호도가 높았다. 추후 다른 재료를 탐색 할 때도 수용성 가용분이 많은 재료가 적합하다고 생각된다. 본 실험에서는 주스 제조 시 침전의 문제를 해결하기 위해 희석과 예열 처리로 응고되는 침전물을 미리 여과하는 방법을 사용했으나 이 보다 더 좋은 개선 방법을 찾기 위한 연구가 앞으로 더 필요하리라 생각된다.

• 약학회지
• /
• 제40권4호
• /
• pp.462-467
• /
• 1996

Forsythiae Fructus was studied on cytotoxic activities for the purpose of finding out active consituents against L1210 and HL60 cells. To isolate the active ones, the methanolic extract was partitioned into water insoluble and water soluble fractions. Furthermore, the water soluble fraction was fractionated into four parts, n-hexane, benzene, ethylacetate and water fractions. Among these, the water insoluble fraction showed the most potent cytotoxic activities on L1210 and HL60 cells in vitro. The water insoluble fraction was applied to silica gel column chromatography and divided into 5 fractions(fr. 1-5). The active constituents I and II were isolated from fr.2 and 3, respectively, by repeated silica gel column chromatography and recrystallization. The constituents were identified as 3${\beta}$-acetylbetulinic acid and betulinic acid by means of physicochemical data. The $ED_{50}$ values of 3${\beta}$-acetylbetulinic acid and betulinic acid were 9.10 and 16.43${\mu}g$/ml against L1210 cells and 2.72 and 2.41${\mu}g$/ml against HL60 cells, respectively.

• 동아시아식생활학회지
• /
• 제22권3호
• /
• pp.392-400
• /
• 2012

The purpose of this study was to analyze the physiological activity and antibiosis of mulberry powder by extract method. The total phenol content of mulberry powder extracted with methanol was 82.9 mg/g, while that of powder extracted with water was 46.9 mg/g. Extractions with methanol were therefore more effective than with water. The total flavonoid contents of mulberry powder extracted with methanol was 13.0 mg/g, while that of powder extracted with water was 9.4 mg/g. Also, the nitrite-scavenging ability of mulberry powder was lower than ascorbic acid and BHT. The SOD-like activity of mulberry powder extracts, natural antioxidant, and artificial antioxidant at 5 mg/mL arranged in order of decreasing concentration were ascorbic acid (98.3%) > BHT (88.1%) > water extract (9.8%) > methanol extract (3.0%). And, the OH radical scavenging activities of mulberry powder extracts and natural antioxidant at 5 mg/mL in order of decreasing concentration was ascorbic acid (97.0%) > methanol extract (46.2%) > water extract (35.8%). The antimicrobial effects of mulberry powder extracted with methanol could be detected on Bacillus cereus ($10,000{\mu}g/disc$) and Staphylococcus aureus ($10,000{\mu}g/disc$).

• Genomics & Informatics
• /
• 제17권4호
• /
• pp.43.1-43.5
• /
• 2019

Lactobacillus acidophilus UBLA-34, L. paracasei UBLPC-35, L. plantarum UBLP-40, and L. reuteri UBLRU-87 were isolated from different varieties of fermented foods. To determine the probiotic safety at the strain level, the whole genome of the respective strains was sequenced, assembled, and characterized. Both the core-genome and pan-genome phylogeny showed that L. reuteri was closest to L. plantarum than to L. acidophilus, which was closest to L. paracasei. The genomic analysis of all the strains confirmed the absence of genes encoding putative virulence factors, antibiotic resistance, and the plasmids.

• Journal of Neurogastroenterology and Motility
• /
• 제24권4호
• /
• pp.669-675
• /
• 2018

Background/Aims Functional dyspepsia (FD) and irritable bowel syndrome (IBS) are common gastrointestinal (GI) disorders and these patients frequently overlap. Trimebutine has been known to be effective in controlling FD co-existing diarrhea-dominant IBS, however its effect on overlap syndrome (OS) patients has not been reported. Therefore, we investigated the effect of trimebutine on the model of OS in guinea pigs. Methods Male guinea pigs were used to evaluate the effects of trimebutine in corticotropin-releasing factor (CRF) induced OS model. Different doses (3, 10, and 30 mg/kg) of trimebutine were administered orally and incubated for 1 hour. The next treatment of $10{\mu}g/kg$ of CRF was intraperitoneally injected and stabilized for 30 minutes. Subsequently, intragastric 3 mL charcoal mix was administered, incubated for 10 minutes and the upper GI transit analyzed. Colonic transits were assessed after the same order and concentrations of trimebutine and CRF treatment by fecal pellet output assay. Results Different concentrations (1, 3, and $10{\mu}g/kg$) of rat/human CRF peptides was tested to establish the OS model in guinea pigs. CRF $10{\mu}g/kg$ was the most effective dose in the experimental OS model of guinea pigs. Trimebutine (3, 10, and 30 mg/kg) treatment significantly reversed the upper and lower GI transit of CRF induced OS model. Trimebutine significantly increased upper GI transit while it reduced fecal pellet output in the CRF induced OS model. Conclusions Trimebutine has been demonstrated to be effective on both upper and lower GI motor function in peripheral CRF induced OS model. Therefore, trimebutine might be an effective drug for the treatment of OS between FD and IBS patients.