• Korean Journal of Veterinary Service
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• v.43 no.2
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• pp.89-97
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• 2020

This study was carried out to examine a novel inactivated Salmonella Typhimurium (S. Typhimurium) vaccine candidate for protection of mice against salmonellosis by immunization of BALB/c mice using various type adjuvant. The novel type-inactivated vaccine candidate was constructed by adding Chlorhexidine digluconate solution. BALB/c mice were divided into 6 groups of 15 mice apiece. The mice were intramuscularly (IM) primed at 6 weeks of age and were IM boosted 8 weeks of age. Groups A and B mice were injected with sterile phosphate-buffered saline as controls; group C mice were inoculated with 5×10⁸ cells/100 µL of formalin-inactivated S. Typhimurium cells and adjuvant ISA70; groups D~F mice were immunized with 5×10⁸ cells/100 µL of the inactivated vaccine candidate and adjuvant ISA70, adjuvant IMS1313 and adjuvant IMS1313 containing 30 ㎍/mL of GI24, respectively. All mice (except group A mice) were orally challenged with a virulent S. Typhimurium strain at 10 weeks of age. Mice from groups C-F had significantly increased IgG levels compared to control groups (A-B) mice. The levels of splenocyte IFN-γ and IL-4 in mice of all groups were measured by ELISA, resulting in increased immunity in group F mice compared to those of groups A-E mice. These data suggested that systemic and cell-mediated immune responses were highly induced by IM immunization with the vaccine candidate and adjuvant IMS1313 containing GI24. Furthermore, clinical signs such as death were observed in only 20% of group F mice after virulent Salmonella strain challenge, however, groups B and C (100%), and groups D and E (60%) mice died. This data suggested that mice immunized by intramuscular prime and booster with this vaccine candidate and adjuvant IMS1313 containing GI24 effectively protected mice from salmonellosis.

• Journal of Mushroom
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• v.2 no.2
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• pp.60-68
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• 2004

Potato dextrose broth was the best inoculation medium to produce in vitro synnemata of Paecilomyces tenuipes. The optimum temperature for synnemata formation of P. tenuipes was $20^{\circ}C$ under 500 lx of light intensity. Highest synnemata production was observed at 18 hr of light period per day. The medium containing 50 g of each silkworm pupae and brown rice produced highest number of synnemata. The optimum ratio of brown rice to distilled water was found as 1:1. Mycelial growth and synnemata production of P. tenuipes was faster and higher in medium containing grinded pupae as compared to whole pupae. The optimum inoculum amount per bottle of medium was 15ml. The highest synnemata production of P. tenuipes was obtained by incubating rice pupae medium at $24^{\circ}C$ until mycelium grows sufficiently after inoculation and then transferring it to $20^{\circ}C$ chamber till harvest.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.253-258
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• 1995

'Ihis experiment was carried out to select a carrier as rhizobium inoculant for alfalfa. The Rhizobium inoculants were produced in main materials(organic and inorganic materials) and submaterial(Ca$CO_3$, and sucrose), and than the changes of Rhizobium population of inoculants were scrutinized from directly after the production to 12 months at $4^{\circ}$C and $25^{\circ}$C. Directly after the production of inoculants, their Rhizobium numbers were 4-9 $\times$ $10^9$/ginoculant in organic materials and 1-8 $\times$ $10^8$/g inoculant in inorganic materials. When they were stored at $25^{\circ}$C, most of inoculants was being started in reduction of rhizobium numbers after 2 months and was reduced by tenfold of initial rhizobium number after 4-6 months, but inoculant of bentonite+ vermiculite+sucrose 1% was not reduced by tenfold after 12 months. When they were stored at $4^{\circ}$C, most of inoculants was being started in reduction of rhizobium numbers after 4 months but was not reduced by tenfold of initial rhizobium number after 12 months, and the effect of sucrose addition to bentonite+ vermiculik did nearly not appear. Preservation of Rhizobium inoculants was more effective at 4C than 25 C . 'Ihe Rhizobium inoalant produced in bentonite+vermiculite(l:3 w/w)+ sucrose 1% was not only high Rhizobium population(8.3 $\times$ $10^8$/g inoculant) but also survival effect of rhizobium in being stored at $25^{\circ}$C.

• Journal of Mushroom
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• v.4 no.2
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• pp.62-67
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• 2006

Pholiota nameko is one of the four major profitable mushrooms along with oak mushroom, winter mushroom, and oyster mushroom. It contains abundant proteins, carbohydrates, organic acids and vitamins. Its unique taste and flavor as well as its nutritional features make it widely favoured. Mushroom complete medium was the optimal medium for mycelial growth of Pholiota nameko. The optimal temperature and pH for the mycelial growth were $25^{\circ}C$ and 5.0, respectively. The best carbon sources for mycelial growth were glucose and mannose, and the best nitrogen sources were yeast extract, peptone, asparagine, etc. The 8:2 ratio mix of oak sawdust and wheat bran was the best for the bottle cultivation. The best mushroom was yielded after 30 days incubation. The best yield was produced with 850g of medium weight in a PP bag and bottle.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.469-472
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• 2002

Solid state fermentation was performed for the production of entomopathogenic fungus Metarhizium anisopliae HY-2 using wheat bran media containing rice bran. Fungal growth in a solid state fermentation system was estimated by viable cell count, spore count, and mycelial biomass. It was used chemical method measuring N-acetyl-glucosamine (chitin) content for estimating of mycelial biomass. In static flask culture, viable cell reached 2.40 ${\times}$ $10^8$ cfu/g at 23 days of culture at $27^{\circ}C$ and then mycelial biomass was 41.59 mg/g. Specific growth rate(${\mu}$ max) was 0.0418 $h^{-1}$ between 3 and 9 days when estimated by viable cell count and was 0.00976 $h^{-1}$ between 9 and 17 days when N-acetylglucosamine content was measured. Viable cells reached 1.12 ${\times}$ $10^8$ cfu/g in polypropylene-bag at 28 days of culture at $27^{\circ}C$. Formulated microbial pesticide containing M. anisopliae HY-2 were tested their bio-activity against Chestnut Brown Chafer (Adoretus tenuimaculatus). The protection rate of the liquid culture showed 13 ${\sim}$ 26 % with 1st to 3rd instar, and spore suspension of M. anisopliae HY-2 showed 56 ${\sim}$ 64%. Conidia produced by large scale solid-state fermentation showed 20 ${\sim}$ 27 % activity 60 ${\sim}$ 64 % with M. anisopliae HY-2.

• Culinary science and hospitality research
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• v.20 no.2
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• pp.246-259
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• 2014

This study was carried out to investigated the quality characteristics of kimchi, made by adding blue crab. Kimchi is made into a fermented vegetable food by adding red pepper powder, garlic, ginger and fermented salted fish to the salted cabbage or radish. Chitin/chitosan, found in the shell of crab is the biopolymer. Chitin possesses many beneficially biological properties. Salt consumption impacts on human health problems such as hypertension and cardiovascular disease. In this study, we hypothesized that kimchi added with raw blue crab would reduce excessive salt consumption and increase protein supplementation. We analyzed lactic acid bacteria level, pH, acidity, salinity and free amino acid of kimchi added with blue crab during 1, 15 and 30 days storage period at $5^{\circ}C$. Lactic acid bacteria, pH, salinity increased significantly in kimchi added with blue crab compared to the control. Accordingly, this result suggested that kimchi should be manufactured by adding raw fish rather than fermented salted fish.

• Journal of Ocean Engineering and Technology
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• v.12 no.2 s.28
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• pp.111-120
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• 1998

Laboratory settling experiments (column, recirculating flume) were conducted for further understanding of the physical processes of cohesive sediment transport. \In still water experiments, the growth rate of flocculation is dependent upon the initial suspended concentration. Consequently, the settling velocity increases with concentration of flees. In flocculation settling regime, the exponent n in the settling velocity, $w_s=kC^n$, for Nakdong estuary mud was obtained empirically. The exponents were found to be 1.33, and 1.06 for the initial suspended concentrations of 1 g/i and 3 g/t, respectively. In flowing water, experiments for the median settling velocity with Nakdong mud in a recirculating flume were conducted. Settling velocity was found to depend much more strongly on the current velocity than initial concentrations. The temporal variation of suspended concentration increases as current velocity decreases.

• Genomics & Informatics
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• v.20 no.1
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• pp.3.1-3.10
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• 2022

The recent development of whole-genome sequencing technologies paved the way for understanding the genomes of microorganisms. Every whole-genome sequencing (WGS) project requires a considerable cost and a massive effort to address the questions at hand. The final step of WGS is data analysis. The analysis of whole-genome sequence is dependent on highly sophisticated bioinformatics tools that the research personal have to buy. However, many laboratories and research institutions do not have the bioinformatics capabilities to analyze the genomic data and therefore, are unable to take maximum advantage of whole-genome sequencing. In this aspect, this study provides a guide for research personals on a set of bioinformatics tools available online that can be used to analyze whole-genome sequence data of bacterial genomes. The web interfaces described here have many advantages and, in most cases exempting the need for costly analysis tools and intensive computing resources.

• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.403-408
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• 2009

A number of bifidobacterial species of human origin were screened for the presence of cryptic plasmids. One strain, Bifidobacterium longum FI10564, harbored plasmids of approximately 2.2 kb, 3.6 kb, and 4.9 kb in size. The smallest plasmid, pFI2576(2,197 bp), was studied in detail and its complete nucleotide sequence was determined. Computer-assisted analysis of this novel plasmid(G+C content 62%) identified 9 putative open reading frames(orfs), 3 of which were shown to be probable genes. These putative genes are arranged in an operon-like structure, in which the overlapping orfs 1 and 2 encode putative Rep proteins and are highly homologous to the rep genes of the B. longum plasmid pMBI(1,847 bp). The mechanism of replication of pFI2576 was investigated using Southern blot analysis of whole cell lysates, with and without S1 nuclease treatment, and atomic force microscopy(AFM). The results indicate that pFI2576 is likely to use the theta mode of replication.

• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.167-177
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• 2005

In oriental medicine, Ostericum koreanum Radix & Aralia contientalis Radix are frequently used materials for strenthing lumbar and knees. Bang-Yak-Hap-Pyun(方藥合編) is widely used for clinical herbal prescription book. In Bang-Yak-Hap-Pyun, Ostericum koreanum Radix is used 1.2g~6g. and Aralia contientalis Radix is used 1.6g~4.8g. The most important fact is that the prescription used Ostericum koreanum Radix & Aralia contientalis Radix at the same time is 11. The number of prescription Ostericum koreanum Radix & Aralia contientalis Radix in Bang-Yak-Hap-Pyun is total 41. 5 in sang-tong(上統), 22 in jung-tong(中統), 14 in ha-tong(下統). The group of mostly used disease is about low back pain, knee pain, C.V.A., facail palsy, commom cold, numbness. The ratio of Ostericum koreanum Radix in Bang-Yak-Hap-Pyun is 3.03%~15.63%. And the ratio of Aralia contientalis Radix is 3.57%~12.00%. The mostly quoted medical literature what prescription included Ostericum koreanum Radix & Aralia contientalis Radix in Bang-Yak-Hap-Pyun is Dong-Yi-Bo-Gam. Man-Byeong-Hoi-Choon, and Hwa-Je-Gook-Bang.