• Korean Journal of Microbiology
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• v.39 no.3
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• pp.128-134
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• 2003

Transformation-associated recombination (TAR) cloning is based on co-penetration into yeast spheroplasts of genomic DNA along with TAR vector DNA that contains 5'- and 3'-sequences (hooks) specific for a gene of interest, followed by recombination between the vector and the human genomic DNA to establish a circular YAC. Typically, the frequency of recombinant insert capture is 0.01-1% for single-copy genes by TAR cloning. To further refine the TAR cloning technology, we determined the effect of GC content on target hooks required for gene isolation utilizing the $Tg\cdot\AC$ mouse transgene as the targeted region. For this purpose, a set of vectors containing a B1 repeated hook and Tg AC-specific hooks of variable GC content (from 18 to 45%) was constructed and checked for efficiency of transgene isolation by radial TAR cloning. Efficiency of cloning decreased approximately 2-fold when the TAR vector contained a hook with a GC content ～${\leq}23$% versus ～40%. Thus, the optimal GC content of hook sequences required for gene isolation by TAR is approximately 40%. We also analyzed how the distribution of high GC content (65%) within the hook affects gene capture, but no dramatic differences for gene capturing were observed.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.631-636
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• 2022

Alcohol content, which is an important standard for Takju, a traditional multiple parallel fermentation liquor called makgeolli, is a factor that can affect the flavor. For alcohol content analysis, the distillation/hydrometry technique is mainly used. In this study, we analyzed the alcohol content of 14 commercially available Takju by the distillation/hydrometry technique and the improved GC method, respectively, after verifying the reliability of improved GC method. The precision and accuracy of the GC method were satisfactory, and LOQ and LOD were evaluated as 0.5% and 0.1% of ethanol contents, respectively. Among the three Takju exceeding the labelled alcohol content ±1, one Takju was quantitated as alcohol content 9.9% (by GC method) and 10.1% (distillation/hydrometry technique) exceeding labelled 6.0%. It was within the analytical error range of alcohol content for other two Takju, where the alcohol contents were exceeded -1.1%. The average precision (%RSD) of 14 Takju analyzed by the distillation/hydrometry technique (36.2%) and the GC method (12.8%), confirming that the GC method was better than the other. The improved GC method was evaluated to be effective in managing and improving the alcohol content standard of Takju with the wide range of alcohol content.

• Proceedings of the Ginseng society Conference
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• 1987.06a
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• pp.89-107
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• 1987

For the elucidation of significance of saponin as quality criterion of ginseng ginsenoside content(GC) and ginsenoside pattern similarity(GPS) by simple correlation were investigated in relation to red ginseng quality factors, age, plant part, harvest season, mineral nutrition, soil physical characteristics, growth light and temperature, shading material, growth location, physiological disease and crop stand through survey of ginseng plantstions, field experiments, water culture and phytotron experiments. Effect of tissue culture was also reviewed. GC was negatively correlated with good quality of red ·ginseng and positively with bad quality. Age did not show any consistency with GC but GPS was less with the increase of age difference. GPS was less or not significant between taproot that is lowest in GC and epidermis highest, and significant between leaf and taproot. Harvest season marked with the lowest GC and Pattern was also different. Nutrient imbalance, the increase of hazardous soil nutrient and physical condition to growth increased GC, but GPS was little different. The higher the growth lights intensity and temperature the higher the GC but GPS was little changed. Root rust increased GC, but root scab decreased it. Sponge-like and inside cavity phenomena increased GC. Ginsenoside pattern of cultured tissues and rootlet showed great variation. These results strongly indicate that there are optimum saponin content and ginsenoside pattern and that these are accomplished under the optimum growth condition.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.767-776
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• 2020

GcGAN is a deep learning model to translate styles between images under geometric consistency constraint. However, GcGAN has a disadvantage that it does not properly maintain detailed content of an image, since it preserves the content of the image through limited geometric transformation such as rotation or flip. Therefore, in this study, we propose a new image-to-image translation method, MSGcGAN(Multi-Scale GcGAN), which improves this disadvantage. MSGcGAN, an extended model of GcGAN, performs style translation between images in a direction to reduce semantic distortion of images and maintain detailed content by learning multi-scale images simultaneously and extracting scale-invariant features. The experimental results showed that MSGcGAN was better than GcGAN in both quantitative and qualitative aspects, and it translated the style more naturally while maintaining the overall content of the image.

• Polymer(Korea)
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• v.27 no.3
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• pp.271-274
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• 2003

A pyrolysis gas-chromatographic method (Py-GC) was utilized for the identification as well as the content measurement of textile materials. Py-GC was applied to natural cotton fiber, synthetic polyester fiber, and their blended fabrics. The characteristic peaks originated from thermally decomposed products were observed, and the area of peak increased with the content of polyester. The products of pyrolized polyester were identified as benzoic acid terephthalic acid, and vinyl benzoic acid, which were characterized by mass spectrometry. This analytic method of offered a quantitative means to identify the content of cotton and polyester.

• ETRI Journal
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• v.38 no.2
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• pp.347-355
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• 2016

In face examinations, gender classification (GC) is one of several fundamental tasks. Recent literature on GC primarily utilizes datasets containing high-resolution images of faces captured in uncontrolled real-world settings. In contrast, there have been few efforts that focus on utilizing low-resolution images of faces in GC. We propose a GC method based on a pixel classifier boosting with modified census transform features. Experiments are conducted using large datasets, such as Labeled Faces in the Wild and The Images of Groups, and standard protocols of GC communities. Experimental results show that, despite using low-resolution facial images that have a 15-pixel inter-ocular distance, the proposed method records a higher classification rate compared to current state-of-the-art GC algorithms.

• Journal of Korea Foundry Society
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• v.7 no.1
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• pp.53-59
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• 1987

In order to study the infuence of Sb addition on the compacted vermicular graphite cast iron(C/V) and gray cast iron(GC), these specimen which had different Sb content(0-0, 1wt.%) were prepared. these specimen were tested for microstructure and mechanical properties. The result from this study are as follows. For C/V and GC, the microstructure of graphite appeared to be finer with increasing Sb content more than 0.06wt.% Ferrite disappeared from the matrix with addition of Sb more than 0.06wt.% in GC and more than 0.08wt.% in C/V. The size of the eutectic cell in GC was 4.5 to 7 times larger than that in C/V, but the decreasing tendancy of its size was more remarkable in GC than in C/V with increasing Sb content. The abrasion speed corresponding to the maximum amount of abrasion in the nabrasion test was found to be 1.37m/sec. GC sample weared 3.6 to 21 times faster than those of C/V did. the amount of abrasion demeased remarkably with Sb addition, and the abrasion rate was constant with Sb content over 0.04wt.%

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.22-22
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• 2021

큰졸방제비꽃(Viola kusanoana)의 엽록체 DNA 염기서열을 밝히고자 차세대염기서열분석법(NGS)을 이용하여 분석하였다. 재료는 경상북도 울릉군 나리분지에 자생하는 개체의 잎을 사용하였다. 염기서열 분석결과, 총 길이는 158,644 bp 였고, GC함량은 36.3%로 분석되었다. 구간별로는 LSC (Large single copy)지역이 86,999 bp (GC content: 33.9%)였고 SSC (Small single copy)지역은 17,439 bp (GC content: 29.9%)으로 분석되었으며 IR (Invertied repeats)지역은 27,103 bp (GC content: 42.2%)로 확인되었다. 유전자는 protein coding gene 77개, tRNA gene 30개, rRNA 4개 등 총 111개로 이는 선행 연구된 제비꽃속 8개 분류군과 유전자의 순서와 방향이 모두 일치하였다. 이를 통해 제비꽃속의 엽록체 게놈의 유전자는 상당히 보존되어 있음을 확인하였다.

• Food Science of Animal Resources
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• v.39 no.4
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• pp.585-600
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• 2019

Gouda cheese, one of most popular cheeses in the Korea, has been produced from only pasteurized milk in Korean dairy farms. Recently, it has become legally possible to produce ripened cheese manufactured with raw milk in Korea. In the present study, we investigated the physico-chemical and microbiological characteristics of Gouda cheese manufactured with raw (R-GC) or pasteurized milk (P-GC) during manufacturing and ripening. Particularly, this study characterized the bacterial community structure of two cheese types, which are produced without pasteurization during ripening based on next generation sequencing of 16S rRNA gene amplicons. During ripening, protein and fat content increased slightly, whereas moisture content decreased in both P-GC and R-GC. At the 6 wk of ripening, R-GC became softer and smoother and hence, the values of hardness and gumminess, chewiness in R-GC was lower than that of P-GC. Metagenomic analysis revealed that the bacterial genera used a starter cultures, namely Lactococcus and Leuconostoc were predominant in both P-GC and R-GC. Moreover, in R-GC, the proportion of coliform bacteria such as Escherichia, Leclercia, Raoultella, and Pseudomonas were detected initially but not during ripening. Taken together, our finding indicates the potential of manufacturing with Gouda cheese from raw milk and the benefits of next generation sequencing for microbial community composition during cheese ripening.

• Journal of Animal Science and Technology
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• v.64 no.3
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• pp.599-602
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• 2022

A new bacteriocin-producing lactic acid bacteria isolated from kimchi was identified as Lactococcus lactis JNU 534, presenting preservative properties for foods of animal origin. In this study, we present the complete genome sequence of the bacterial strain JNU 534. The final complete genome assembly consists of one circular chromosome (2,443,687 bp [base pair]) with an overall GC (guanine-cytosine) content of 35.2%, one circular plasmid sequence (46,387bp) with a GC content of 34.5%, and one circular contig sequence (7,666 bp) with a GC content of 36.2%.