• Title/Summary/Keyword: GBL

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Characterization of Lactobacillus plantarum strains isolated from black raspberry and their effect on BALB/c mice gut microbiota

  • Choi, Hye Ran;Chung, Yi Hyung;Yuk, Hyun-Gyun;Lee, Hyunki;Jang, Han Su;Kim, Yosum;Shin, Daekeun
    • Food Science and Biotechnology
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    • v.27 no.6
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    • pp.1747-1754
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    • 2018
  • The objective of this study was to evaluate probiotic effects of two Lactobacillus plantarum strains (GBL16 and 17) isolated from black raspberry. Results revealed that the number of GBL16 was gradually decreased as bile salt concentration was increased from 0.3 to 1%. However, GBL17 did not show any difference when GBL17 was applied to 1% bile salt, and it indicates that GBL17 is more tolerant to bile salt than GBL16. GBL17 exhibited higher heat resistance and adhesion ability to Caco-2 cells than GBL16. Regarding gut microbiome, no significant change in the number of total bacteria in intestines of mice after treatment with GBLs was determined. However, the combination of GBL16 and GBL17 significantly increased the number of total bacteria in intestines of mice after they were orally administered. Therefore, the results suggest that both GBL16 and 17 strains could be one of major probiotics that can improve human gut health.

Organic Electrolyte of the Additive the Gamma-Butyroloctone (GBL) for Additive Material Application to High Voltage Electrochemical Capacitor (Gamma-butyroloctone(GBL)을 첨가한 유기계 전해액의 고전압용 전기화학 커패시터로의 응용)

  • You, Sun-Kyung;Park, Soo-Gil
    • Journal of the Korean Electrochemical Society
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    • v.20 no.1
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    • pp.13-17
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    • 2017
  • In this study, we studied the organic electrolyte application to electrochemical capacitor for high operation voltage. For high operating voltage, 5 wt % of gamma butyroloctone (GBL) was added in the bare electrolyte. During the cycle performance, stable SEI layers were formed by reductive decomposition of additive GBL. As a result, columbic efficient of 1M $SBPBF_4$ in EC:DMC(1:1) with GBL composite was enhanced to 70% after the 2000th cycle at voltage range 0-3.5 V. Additionally, SEI layer protected the surface of electrode and prevent the side-reaction between electrolyte to electrode.

Electrochemical Properties of Trimethylammonium Tetrafluoroborate in Electrochemical Double-Layer Capacitors

  • Lee, Sooyeon;Lee, Kyung Min;Kim, Ketack
    • Journal of Electrochemical Science and Technology
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    • v.13 no.2
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    • pp.254-260
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    • 2022
  • Trimethylammonium tetrafluoroborate (TriMA BF4), consisting of the smallest trialkylammonium ion, was investigated for use in electrochemical double-layer capacitors. Despite the presence of a proton in TriMA+, cycle life tests in acetonitrile (AN) and -butyrolactone (GBL) showed a good capacity retention with a 1.8 V cut-off voltage. The rate of electrolysis of TriMA BF4 in GBL was lower than that in AN because of the lower conductivity in GBL. As a consequence, the cells based on GBL achieved a higher capacitance and longer life than those with AN. TriMA BF4 had a higher conductivity and lower viscosity than the quaternary salt tetraethylammonium tetrafluoroborate in GBL, as well as higher ionic mobility, these factors resulted in a higher rate capability.

Games Application Methodology for History Education: Case Study of Developing a Serious Game for History Education (역사교육에 대한 게임 활용 방안: 기능성 게임 개발사례를 중심으로)

  • Jung, ChanYong
    • Journal of Korea Game Society
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    • v.18 no.6
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    • pp.29-38
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    • 2018
  • Although computer games seem to be efficient tools for facilitating and supporting situated learning in Europe, GBL(game based learning) is less likely in Korea. The objectives of this paper are to address the causes of the problem and evaluate a serious game for history GBL. We review various cases of history GBL projects in European School Net, commercial games and serious games which are related to history. We draw a demand of our game's structural type, learning criterion, basic model of instruction, and executable prototype from the analysis results. Scene management educators pursue and dependencies of turning points in the history are critical differentiator. An evaluation team of educators, learners, and edu-game managers evaluates that the prototype is suitable model for application in history instruction.

Effect of Addition of Cosolvent γ-Butyrolactone on Morphology of Polysulfone Hollow Fiber Membranes (폴리설폰 중공사막 구조에 대한 조용매 γ-Butyrolactone 첨가 영향)

  • Yun, Sukbok;Lee, Yongtaek
    • Applied Chemistry for Engineering
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    • v.25 no.3
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    • pp.274-280
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    • 2014
  • Polysulfone (PSf) hollow fiber membranes were prepared via the nonsolvent induced phase separation technique. The cosolvent of ${\gamma}$-butyrolactone (GBL) was added to the polymer solution containing a mixture of PSf and N,N-dimethylacetamide (DMAc). Water was utilized as a precipitation nonsolvent. The morphology of prepared membranes was investigated using a field emission scanning electron microscopy. The fabricated membrane showed a typical asymmetric structure such as the dense layer on the porous support layer by the addition of GBL to the polymer solution. As the concentration of GBL increased, the asymmetric porous structure was shown to be more intensified. It was thought that the added GBL played a role of enhancing the liquid-liquid phase separation of the polymer solution, since the cosolvent of GBL might change the thermodynamic solubility parameter of the doping solution. Permeation properties through the prepared hollow fiber membranes were characterized by measuring the pure water flux and the solute rejection using $0.05{\mu}m$ polystyrene latex (PSL) beads. Experimental results revealed that the use of PEG as the internal coagulant enhanced the pure water flux up to 130 times compared to the use of EG while the rejection of the PSL beads decreased only 5%.

Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae) (쌍별귀뚜라미(Gryllus bimaculatus)의 l(2)efl cDNA 클로닝과 발현분석)

  • Kwon, Kisang;Lee, Nuri;Kwon, O-Yu
    • Journal of Life Science
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    • v.31 no.7
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    • pp.671-676
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    • 2021
  • A cDNA encoding the protein lethal (2) essential for life [l(2)efl] was cloned from Gryllus bimaculatus and named GBl(2)efl. This protein is composed of 189 amino acids, including an N-glycosylation site and 15 phosphorylation sites. Its predicted molecular mass is 21.19 kDa, with a theoretical isoelectric point of 6.2. The secondary structure of GBl(2)efl was predicted from the identification of random coils (56.08%), alpha helices (22.22%), extended strands (17.99%), and beta turns (3.7%) through sequence analyses. A homology analysis revealed that GBl(2)efl exhibited a high similarity with other species at the amino acid level, ranging from 52% to 69%. While GBl(2)efl mRNA expression was higher in the dorsal longitudinal flight muscle following a three-day starvation and in the Malpighian tubules following a one-day starvation, no changes in expression were detected in other tissues. Furthermore, tunicamycin-induced endoplasmic reticulum (ER) stress resulted in an approximately 1.8-fold higher expression in the fat body compared with the wild type.

Content Development by Combining Intelligent Tutoring and Game-based Learning (지능형 튜토링과 게임 기반 학습을 결합한 콘텐츠 개발)

  • Hong, Myoung-Pyo;Han, Ki-Tae;Lee, Eui-Hyeock;Choi, Yong-Suk
    • Journal of KIISE:Computing Practices and Letters
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    • v.16 no.5
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    • pp.601-605
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    • 2010
  • In this paper, we propose a GBL(Game Based Learning) content of intelligent tutoring capability. The objective of our GBL content is to learn the Karnaugh Map which is generally used to simplify boolean functions. Our GBL content well-motivates learners with interesting game-based scenarios and also, through an intelligent tutoring module, gives learners adaptive feedbacks such as hints and explanations while maintaining learners' contextual immersion. Additionally, we identified significant improvement in terms of learning effectiveness by analyzing the test results of two (experimental and controlled) student groups learning the Karnaugh Map.

Microstructure and Dielectric Properties of a SrTiO3-based GBL Capacitor (SrTiO3계 GBL Capacitor의 미세구조 및 유전특성)

  • 천채일;김호기
    • Journal of the Korean Ceramic Society
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    • v.24 no.3
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    • pp.270-276
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    • 1987
  • The microstructure and dielectric properties of a SrTiO3-based GBL (Grain Boundary Layer) capacitor were investigated. The 0.6 mol% Nb2O5 doped SrTiO3 was sintered for 3 hr at 1450$^{\circ}C$ in mixed gas(N2/H2) atmosphere. The Nb2O5 promoted the grain growth of the SrTiO3 ceramics was decreased with the amount of Nb2O5. The oxide mixture(PbO, Bi2O3, B2O3) were painted on the reduced specimen and fired at 1000$^{\circ}C$ to 1100$^{\circ}C$ in air. The penetrated oxide mixture into specimen were located in grain boundaries. A SrTiO3-based GBL capacitor had the apparent permittivity of about 3.0${\times}$104, the dielectric loss of 0.01-0.02, and insulating resistance of 108-109$\Omega$.cm. The capacitor had the stable temperature coefficient of capacitance and exhibited dielectric dispersion over 107 Hz. The capacitance-voltage measurements indicated that the grain boundary was composed of the continuous insulating layers.

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Characteristics of Lactic Acid Fermentation of Black Raspberry Juice Using the Lactobacillus plantarum GBL17 Strain (Lactobacillus plantarum GBL17 균주를 이용한 복분자 유산발효 특성)

  • Ryu, Eun-Hye;Yoon, Hae-Hoon;Jung, Ji-Hye
    • Korean journal of food and cookery science
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    • v.31 no.6
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    • pp.773-780
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    • 2015
  • Lactic acid fermentation of black raspberry (Rubus occidentalis) juice was carried out by using the Lactobacillus plantarum GBL17 strain. The sterilized black raspberry juice was fermented using the L. plantarum GBL17 strain at $30^{\circ}C$ for 72 hours after which the total acidity increased and the pH value decreased. In addition, the highest total acidity content (2.38%) was reached, the lowest pH value (3.22) was observed, and the sugar content decreased by $9.8^{\circ}Brix$ after the 72 hour fermentation. The number of viable cells rapidly increased up until 24 hours, after which it gradually decreased. HPLC analysis of the organic acids showed 14.51 mg/g of lactic acid content in the fermented black raspberry juice, which was not detected in the non-fermented black raspberry juice (control). The content of fructose and glucose slightly decreased after fermentation. The total polyphenol and flavonoid contents of the fermented black raspberry juice increased significantly after fermentation. The DPPH radical scavenging activity of fermented black raspberry juice (70.92%) was higher than that of the control (62.96%). After lactic acid fermentation, there was no significant increase in ABTS radical scavenging activity. These results confirm that lactic acid bacteria, such as L. plantarum GBL17, showed generally higher activities with a potential as a functional beverage.