• Korean Journal of Food Science and Technology
• /
• v.41 no.5
• /
• pp.597-601
• /
• 2009

Activation of hepatic stellate cells (HSCs) is known to be responsible for hepatic fibrosis and cirrhosis. When round-shape quiescent HSCs go to activation by liver injury, production of extracellular matrix is increased, and its shape becomes myofibroblast-like shape. The activated HSCs are characterized by the high rate of proliferation and the increased production of extracellular matrix. One way of the regeneration of activated HSCs is an apoptosis induction followed by removing the activated myofibroblast-like cells. The effect of extract of Terminalia chebula Retz. (TCE) on cytotoxicity was evaluated using the rat primary hepatocyte, HepG2 and T-HSC/Cl-6 by incubating these cells with TCE up to the dose of $1,000{\mu}g/mL$. At the maximum dose of TCE, no cytotoxicity was found on primary hepatocyte and HepG2, but cytotoxic effect of TCE was found on activated HSCs, and T-HSC/Cl-6 in a U-shaped dose-response manner with the highest effect at $500{\mu}g/mL$ of TCE. Finally, we confirmed the occurrence of apoptotic cell death by annexin-V/PI double staining. The population of annexin-V positive cells was increased in a dose dependent manner.

• Research in Community and Public Health Nursing
• /
• v.14 no.4
• /
• pp.587-597
• /
• 2003

Purpose: The purpose of this study was to estimate the demand and supply of visiting nursing services provided by health centers in urban area, aiming at strengthening infrastructure, which may improved the quality of life and health status of vulnerable population in the community. Methods: This study was conducted through nominal group discussion, focus group study. The demand and supply of visiting nursing were estimated by health economists based on the secondary analysis data from 25 health centers in Seoul. Result: Primary targets for the visiting nursing must be people who are homebound in the community. They can be classified into: a group of Level I: chronic patients who need visiting nursing care at least once a week: and a group of Level II: vulnerable families that need management periodically e. g. twice a month. Based on the estimation of demand for visiting nursing services in the community, the estimated supply required was $651{\sim}770$ visiting nurses including home health nurses in visiting nursing programs based on health centers in Seoul. Conclusions: The estimated demand and supply of visiting nursing are expected to provide basic data for establishing alternative policies on visiting nursing infrastructure that might be accomplished through demand-based visiting nursing programs by districts.

• Journal of Nutrition and Health
• /
• v.29 no.6
• /
• pp.597-607
• /
• 1996

This study has been investigated the potenial of increased dietary cysteine to alter the effects of cadmium and lead on tissure and bone metal concentrations, excretion and tissue metallothionein(MT) concentrations. Fifty-four male rats of Sprgue-Dawley strain weighing 149$\pm$17g were divided into 9 groups according to body weight. Nine experimental diets with different cadmium (0ppm, 400ppm), lead(0ppm, 710ppm) and cysteine (0.06%, 0.45%, 0.90%) levels were given to rats for 30 days ; Food intake, weight gain, F.E.R, and weights of liver, kidney and femur were decreased in cadmium supplied groups than in cadmium free groups. Urinary and fecal cadmium excretions were increased and MT synthesis we induced in liver, kidney and small intestine in cadmium supplied groups. In lead supplied groups, weight gain and F.E.R were decreased. With cysteine supplementation in cadmium supplied groups, weight gain and F.E.R, and weights of liver, kidney and femur were increased. Cadmium excretion in feces and MT concentrations in liver and kidney were also increased with cysteine supplementation. In lead supplied groups, there was no significant increase in food intake, weight gain and F.E.R with cysteine supplementation. Lead excretion in feces was increased in cysteine supplemented groups. In conclusion, effect of cadmium administration was more toxic than lead adminstration. Cysteine alleviated cadmium and lead toxicity by increasing metallothionein concentration and fecal excretions of heavy metals. Especially, effect of cysteine supplementation was more effective in cadmium groups than in lead groups. Effect of cysteine supplementation was not different with level of cysteine supplementation in both cadmium and lead groups.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.4
• /
• pp.591-597
• /
• 2003

Human urokinase kringle domain, sharing homology with angiostatin kringles, has been shown to be an inhibitor of angiogenesis, which can be used for the treatment of cancer, rheumatoid arthritis, psoriasis, and retinopathy. Here, the expression of the kringle domain of urokinase (UK1) as a secreted protein in high levels is reported. UK1 was expressed in the methylotrophic yeast Pichia pastoris GS115 by fusion of the cDNA spanning from Ser47 to Lys135 to the secretion signal sequence of ${\alpha}-factor$ prepro-peptide. In a flask culture, the secreted UK1 reached about 1 g/l level after 120h of methanol induction and was purified to homogeneity by ion-exchange chromatography. Amino-terminal sequencing of the purified UK1 revealed that it was cleaved at the Ste13 signal cleavage site. The molecular mass of UK1 was determined to be 10,297.01 Da. It was also confirmed that the purified UK1 inhibited endothelial cell proliferation stimulated by basic fibroblast growth factor, vascular endothelial growth factor, or epidermal growth factor, in a dose-dependent manner. These results suggest that a P. pastoris sytem can be employed to obtain large amounts of soluble and active UK1.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2005.03a
• /
• pp.597-602
• /
• 2005

The present precast top-base method create many problems of requiring it plant facilities, transportation and installation, due to the heavy weight of and it takes too long time to set it up on site. In order to improve and solve these problems, in-situ Top-Base method is developed. It include processes that install Top-Base mold made of poly-ethylene into ground, then pouring concrete into the mold, and fill the rest gaps with broken stones. Considerable advantages can be obtained by applying in-situ Top-Base method in aspects of the stability, economical and construction efficiency. In this research, model tests for in-situ Top-Base system are carried out in other to the investigate the load delivering mechanism and the effect of bearing capacity.

• Proceedings of the KIEE Conference
• /
• summer
• /
• pp.597-601
• /
• 2004

HVDC(High Voltage Direct Current) is an underwater cable between Jeju Island and Haenam in main land and supplies approximately $50\%$ of electrical usage in Jeju Island. If there is any power failure due to HVDC, it will cost approximately 50,000 US dollars per day including Thermal Electrical Generation. Therefore it is absolutely necessary to recover the problem in rapid timely basis. Present survey method in Korea is done by scuba diver with air cylinder resulting very poor visual inspection. Other option is by only visual camera attached on miniature ROV for solely suey Purpose. This method does not includeburial depth of cable, cable position, cable condition & etc??‥‥.??? In result, current method does not generate any scientific or sophisticated data which does not allow any intelligent management decision. In conclusion, new method and new systems are needed urgently to upgrade current HVDC underwater cable survey technique in Korea to minimize the cost and time factors.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.28 no.1
• /
• pp.7-13
• /
• 2020

A significant growth in local air traffic volume is leading to airway congestion and flight delays especially for Incheon-China and Incheon-Europe sectors. A key method to increase the airway capacity is to place a supplemental airway parallel to the existing one and in cooperation between the aviation authorities between China and Korea, a dual airway track was implemented on December 6, 2018. Here, we use airline A's flight data to analyze the congestion change effect of the new airway. Results show total delay time to Europe is reduced 51% (13.4 to 6.6 minutes) as the delay distribution for 16-30 minutes, 31 minutes and greater decreased from 23.2% to 8.2% and 8.7% to 1.0% respectively. The delay to China also decreased but the drop is not as significant as flights to Europe. This is caused by the difference in flight distance, traffic volume, and characteristics of flights landing and transiting China. Flights to Europe show a broad distribution in altitude allocation and reduction in aircraft separation demonstrating the effectiveness of a dual airway track.

• Korean Journal of Materials Research
• /
• v.13 no.9
• /
• pp.593-597
• /
• 2003

Anodic aluminum oxide (AAO) membrane was made of aluminum sheet (99.6%, 0.2 mm thickness). The regular array of hexagonal nano pores or channels were prepared by two step anodization process. A detail description of the AAO fabrication is presented. After the 1st anodization in oxalic acid (0.3 M) at 45 V, The formed AAO was removed by etching in a solution of 6 wt% $H_3$$PO_4$＋1.8 wt% $H_2$$CrO_4$. The regular arrangement of the pores was obtained by the 2nd anodization, which was carried out in the same condition as the 1st anodization. Subsequently, the alumina barrier layer at the bottom of the channel layer was removed in phosphoric acid (1M) after removing of aluminum. Pore diameter, density, and thickness could be controlled by the anodization process parameters such as applied voltage, anodizing time, pore widening time, etc. The pore diameter is proportional to the applied voltage and pore widening time. The pore density and thickness can be controlled by anodization temperature and voltage.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2005.10a
• /
• pp.593-597
• /
• 2005

FIB equipment can perform sputtering and chemical vapor deposition simultaneously. It is very advantageously used to fabricate a micro structure part having 3D shape because the minimum beam size of ${\phi}$ 10nm and smaller is available. Currently FIB is not being applied in the fabrication of this micro part because of some problems to redeposition and charging effect of the substrate causing reduction of accuracy with regards to shape and productivity. Furthermore, the prediction of the material removal rate information should be required but it has been insufficient for micro part fabrication. The paper have the targets that are FIB-CVD characteristic analysis and minimum line pattern resolution achievement fur 3D micro fabrication. We make conclusions with the analysis of the results of the experiment according to beam current, pattern size and scanning parameters. CVD of 8 pico ampere shows superior CVD yield but CVD of 1318 pico ampere shows the pattern sputtered. And dwell time is dominant parameter relating to CVD yield.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.17 no.1
• /
• pp.55-60
• /
• 1984

An experiment on the efficiency of soybean meal substituted for fish meal in the diet of common carp was carried out at the Fish Culture Experiment Station of the National Fisheries University of Pusan from June 27 to August 25, 1983. The carp used for the experiment were 50 fish each lot, each averaging 26 g in initial weight (total weight 1300 g). The experimental diets contained $10\%,\;20\%,\;30\%$, and $40\%$ soybean meal ($44\%$-protein) at iso-protein content basis in place of white fish meal in the control diet which contained $35\%$ protein. The growth rate in the control division was highest with progressive decrease in performance of the fish with increasing rates of soybean meal substituted in the test diets. Nevertheless, the $10\%\;and\;20\%$ soybean meal test diet groups showed acceptable conversions and growth rates, i. e. feed coefficients being 1.28 and l.30 and daily growth rates, $1.708\%\;and\;1.694\%$ compared to $1.22\%\;and\;1.758%$ for the control group, respectively. Therefore, soybean meal could be substituted for fish meal in carp diet by $20\%$ without significant differences of performance with some saving in feed cost.