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Major gene identification for SREBPs and FABP4 gene which are associated with fatty acid composition of Korean cattle (한우의 지방산 조성에 영향을 미치는 SREBPs와 FABP4의 유전자 조합 규명)

  • Lee, Jae-Young;Jang, Ji-Eun;Oh, Dong-Yep
    • Journal of the Korean Data and Information Science Society
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    • v.26 no.3
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    • pp.677-685
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    • 2015
  • Disease of human and economic traits of livestocks are affected a lot by gene combination effect rather than a single gene effect. In this study, we used SNPHarvester method that supplement existing method in order to investigate the interaction of these genes. The used genes are SREBPs (g.3270+10274 C>T, g.13544 T>C) and FABP4 (g.2634+1018 A>T, g.2988 A>G, g.3690 G>A, g.3710 G>C, g.3977-325 T>C, g.4221 A>G) that are closely related to the fatty acid composition affecting the meatiness of Korean cattle. The economic traits which are used are oleic acid (C18:1), monounsaturated fatty acid (MUFA), marbling score (MS). First, we have utilized the SNPHarvester method in order to find excellent gene combination, and then used the multifactor dimensionality reduction method in order to identify excellent genotype in gene combination.

Introducing Software Defined Radio to 4GWireless: Necessity, Advantage, and Impediment

  • Zamat, Hassan;Nassar, Carl R.
    • Journal of Communications and Networks
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    • v.4 no.4
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    • pp.344-350
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    • 2002
  • This work summarizes the current state of the art in software radio for 4G systems. Specifically, this work demonstrates that classic radio structures, e.g., heterodyne reception, homodyne reception, and their improved implementations, are inadequate selections for multi-mode reception. This opens the door to software defined radio, a novel reception architecture which promises ease in multi-band, multi-protocol design. The work presents the many advantages of such an architecture, including flexibility, reduced cost via component reduction, and improved reliability via, e.g., the elimination of environmental instability. The work also explains the limitations that currently curtail the widespread use of SDR, including issues surrounding A/D converters, management of software and power, and clock generation. This provides direction for future research to enable the broad applicability of SDR in 4G cellular and beyond.

Isolation and Characterization of a Fungus Which Produce Antimicrobial Substance (I) (항생물질을 생산하는 곰팡이의 분리 및 그 생산조건 (제1보))

  • Sung, Chang Keun;Kim, Chan Jo
    • Korean Journal of Agricultural Science
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    • v.11 no.1
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    • pp.53-60
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    • 1984
  • The followings are the results obtained from the investigation of isolation and cultural conditions of a fungus producing antibiotic from humus, spring soil, paddy, field and etc. 1. Among fungi which were capable of antimicrobial sub stance producing at the $45^{\circ}C$, Strain H-3 was selected as test st rain because of its efficiency in antimicrobial substance producing. 2. Strain H-3 was identified as a Aspergullus fumigatus by the mophological properties. 3. The compositions of optimun media for the production of antimicrobial substance were followed;glucose 20g, $NaNO_3$ 3g, $K_2HPO_4$ 1g, $CaCl_2$ 0.5g, $MgSo_4$ 0.5g, $FeSO_4$ 0.005g. 4. Optimum culture conditions were founded to be pH 5.0, $40^{\circ}C$ respectively. 5. The maximum antimicrobial substance producing obtained in this study was showed 14mm of clear zone after 72 hrs incubation under the optimum conditions. 6. AF-c substance was active against Gram-positive, Gram-negative bacteria, and yeast but not mold in its biological properties.

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Effect of ascorbic acid, ethanol and acetone on adhesion between the treated fiber posts and composite resin cores

  • Zahra, Khamverdi;Reza, Talebian
    • The Journal of Advanced Prosthodontics
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    • v.4 no.4
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    • pp.187-191
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    • 2012
  • PURPOSE. The aim of the present study was to assess the effect of ascorbic acid, ethanol and acetone on microtensile bond strength between fiber posts pre-treated with hydrogen peroxide and composite resin cores. MATERIALS AND METHODS. Twenty four fiber posts were pre-treated with 24% hydrogen peroxide and divided into 4 groups as follows: G1: no treatment, as control group; G2: treatment with10% ascorbic acid solution for 5 minutes; G3: treatment with 70% ethanol solution for 5 minutes; and G4: treatment with 70% acetone solution for 5 minutes. Each fiber post was surrounded by a cylinder-shaped polyglass matrix which was subsequently filled with composite resin. Two sections from each sample were selected for microtensile test at a crosshead with speed of 0.5 mm/min. Statistical analyses were performed using one-way ANOVA and a post hoc Tukey HSD test. Fractured surfaces were observed under a stereomicroscope at ${\times}20$ magnification. The fractured surfaces of the specimens were observed and evaluated under a SEM. RESULTS. Means of microtensile bond strength values (MPa) and standard deviations in the groups were as follows: G1: $9.70{\pm}0.81$; G2: $12.62{\pm}1.80$; G3: $16.60{\pm}1.93$; and G4: $21.24{\pm}1.95$. G4 and G1 had the highest and the lowest bond strength values, respectively. A greater bond strength value was seen in G3 compared to G2. There were significant differences between all the groups (P<.001). All the failures were of the adhesive mode. CONCLUSION. Application of antioxidant agents may increase microtensile bond strength between fiber posts treated with hydrogen peroxide and composite cores. Acetone increased bond strength more than ascorbic acid and ethanol.

IgG subclass-dependent Recognition of Porphyromonas Gingivalis Antigens in the Early-onset Periodontitis (조기발병형 치주염환자의 IgG subclass 별 Porphyromonas gingivalis 항원인지에 대한 연구)

  • Choi, Jeom-Il;Yoshimobu, Fuminobu;Schifferle, Robert E.;Okuda, Katsuji
    • Journal of Periodontal and Implant Science
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    • v.29 no.4
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    • pp.953-964
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    • 1999
  • 본 연구는 세 종류의 Porphyromonas gingivalis(Pg) antigen의 IgG subclass associated recognition을 평가하기 위해 수행했었다. 총 35명의 조기발병형치주질환자중, Pg381에 대한 IgG2항체의 증가를 보이는 5명이 급속진 행형 치주질환자, IgG4의 증가를 보이는 6명의 환자(국소유년형 치주질환자 2명과 급속진행 형 치주질환자 4명), IgG2+4의 증가를 보이는 2명의 급속진행형 치주질환자 그리고 IgG1+2+4의 증가를 보이는 8명의 환자(국소 유년형 치주질환자 2명과 급속진행형 치주질환자 6명)으로 구성된 21명의 환자를 dot immunoblot analysis를 위해 선택했다. 실험에 사용된 정제된 항원은 Pg381에서 추출한 43-kd fimbrilin protein과 lipoplysaccharide(LPS), Pg A7A1-28(ATCC 53977)에서 추출한 capsularpolysaccharide(CPS)였다. Immunoblotting pattern은 IgG4 antibody가 fimbrial antigen에 강력히 반응함을 보여주었다. Fimbriae에 잘 반응하는 몇몇의 IgG4 antibody역시 antigen에 대해 양성반응을 보였다. 대조적으로 IgG2는 CPS antigen을 일차적으로 인식했다. 전부는 아니지만 대부분의 경우, single이나 group화된 IgG subclass는 모두 LPS antigen을 인식하지 못했다. 같은 group에서 염색강도의 개인적인 차이는 증명되었다. 이런 결과는 조기발 병형 치주질환에서 Pg의 fimbriae와 CPS가 immunodominant antigen이 될 수 있음을 제시한다. 더욱이 IgG subclass antibody가 이런 Pg의 immunoglobulin antigen을 선택적으로 인식함을 알았고, 이는 조기발병형치주질환의 병리에 immunodominant antigen과 함께 IgG의 기능적인 역할을 고려해야 함을 제시한다.

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Strategy Promoting 5G N/W Investment in UK (영국의 5G N/W 투자촉진 전략)

  • Byun, J.H.
    • Electronics and Telecommunications Trends
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    • v.32 no.4
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    • pp.89-95
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    • 2017
  • 영국은 2017년 3월 5G N/W 조기구축을 위한 정부차원의 전략방향 보고서(A 5G Strategy for UK)를 발표하였다. 영국은 5G 인프라가 4차 산업혁명의 촉진제 역할을 할 것이며, 5G로 인한 경제적 파급 효과가 2030년경에 1,980억 파운드 달할 것으로 예상한다. 이러한 전망에 따라 영국 정부는 5G 테스트베드 구축, Small Cell 및 백홀 구축 지원, 주파수 정책방향 제시 등 5G N/W 조기구축 환경을 조성하여 이동통신사의 적극적인 투자를 유인할 계획이다. 아울러 산업계, 학계, 지역정부와 협력하여 다양한 산업분야에서의 5G 활용 방안을 도출하며, 5G 상용화 이전에 해결해야 할 과제를 정비함으로써 영국이 5G 분야에서 글로벌 리더가 되고자 하는 목표를 제시하고 있다. 본고에서는 영국이 4차 산업혁명에 대비하여 제시하는 5G N/W 투자 촉진 전략을 소개하고자 한다.

THE STRUCTURE OF A CONNECTED LIE GROUP G WITH ITS LIE ALGEBRA 𝖌=rad(𝖌)⊕ 𝔰𝒍(2,𝔽)

  • WI, MI-AENG
    • Honam Mathematical Journal
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    • v.17 no.1
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    • pp.7-14
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    • 1995
  • The purpose of this study is to construct the structure of the connected Lie group G with its Lie algebra $g=rad(g){\oplus}sl(2, \mathbb{F})$, which conforms to Stellmacher's [4] Pushing Up. The main idea of this paper comes from Stellmacher's [4] Pushing Up. Stelhnacher considered Pushing Up under a finite p-group. This paper, however, considers Pushing Up under the connected Lie group G with its Lie algebra $g=rad(g){\oplus}sl(2, \mathbb{F})$. In this paper, $O_p(G)$ in [4] is Q=exp(q), where q=nilrad(g) and a Sylow p-subgroup S in [7] is S=exp(s), where $s=q{\oplus}\{\(\array{0&*\\0&0}\){\mid}*{\in}\mathbb{F}\}$. Showing the properties of the connected Lie group and the subgroups of the connected Lie group with relations between a connected Lie group and its Lie algebras under the exponential map, this paper constructs the subgroup series C_z(G)

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Correlation analyses of CpG island methylation of cluster of differentiation 4 protein with gene expression and T lymphocyte subpopulation traits

  • Zhao, Xueyan;Wang, Yanping;Guo, Jianfeng;Wang, Jiying
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1141-1149
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    • 2018
  • Objective: Cluster of differentiation 4 protein (CD4) gene is an important immune related gene which plays a significant role in T cell development and host resistance during viral infection. Methods: In order to unravel the relationship of CpG island methylation level of CD4 gene with its gene expression and T lymphocyte subpopulation traits, we used one typical Chinese indigenous breed (Dapulian, DP) and one commercial breed (Landrace), then predicted the CpG island of CD4 gene, determined the methylation status of CpG sites by bisulfite sequencing polymerase chain reaction (BSP), and carried out the correlation analyses of methylation frequencies of CpG sites with mRNA expression and T lymphocyte subpopulation traits. Results: There was one CpG island predicted in the upstream -2 kb region and exon one of porcine CD4 gene, which located 333 bp upstream from the start site of gene and contained nine CpG sites. The correlation analysis results indicated that the methylation frequency of CpG_2 significantly correlated with CD4 mRNA expression in the DP and Landrace combined population, though it did not reach significance level in DP and Landrace separately. Additionally, 15 potential binding transcription factors (TFs) were predicted within the CpG island, and one of them (Jumonji) contained CpG_2 site, suggesting that it may influence the CD4 gene expression through the potential binding TFs. We also found methylation frequency of CpG_2 negatively correlated with T lymphocyte subpopulation traits CD4+CD8-CD3-, CD4-CD8+CD3- and CD4+/CD8+, and positively correlated with CD4-CD8+CD3+ and CD4+CD8+CD3+ (for all correlation, p<0.01) in DP and Landrace combined population. Thus, the CpG_2 was a critical methylation site for porcine CD4 gene expression and T lymphocyte subpopulation traits. Conclusion: We speculated that increased methylation frequency of CpG_2 may lead to the decreased expression of CD4, which may have some kind of influence on T lymphocyte subpopulation traits and the immunity of DP population.

Analysis of Nucleotides and Their Derivatives in Renal Tissue of Rat during Ischemia by HPLC (흰쥐의 신허혈에서 HPLC를 이용한 핵산대사산물의 분석)

  • Kim, Seong-Yong;Kim, Jung-Hye
    • Journal of Yeungnam Medical Science
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    • v.9 no.1
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    • pp.90-101
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    • 1992
  • In rat kidney, the changes in concentrations of nucleotides and their derivatives during ischemia induced by renal artery ligation was measured quantitatively with high performance liquid chromatography(HPLC). After the ligation of renal artery for 60minutes, the concentrations of the nucleotides and derivatives were measured. In ischemic tissue, IDP was significantly decreased from $217.4{\pm}12.68{\mu}g$ in control to $80.7{\pm}18.39{\mu}g$ (p<0.01) ; ATP, $307.2{\pm}56.63{\mu}g$ to $47.6{\pm}5.95{\mu}g$ (p<0.01) ; ADP+AMP, $227.1{\pm}7.98{\mu}g$ to $61.4{\pm}3.92{\mu}g$(P<0.01); $NAD^+$, $217.9{\pm}4.49{\mu}g$ to $126.6{\pm}10.44{\mu}g$(P<0.01) ; GTP, $202.5{\pm}23.76{\mu}g$ to $117.7{\pm}14.24{\mu}g$ (P<0.05) ; GMP, $54.5{\pm}9.03{\mu}g$ to $23.7{\pm}0.46{\mu}g$(p<0.05), and inosine, $16.6{\pm}3.45{\mu}g$ to $7.8{\pm}0.87{\mu}g$ (P<0.05). But hypoxanthine and xanthine were significantly increased from $113.0{\pm}15.58{\mu}g$ to $159.7{\pm}12.07{\mu}g$ (P<0.05) and from $87.7{\pm}6.77{\mu}g$ to $173.1{\pm}12.52{\mu}g$ (P<0.01). In ischemic kidney, concentration of ATP was decreased to 39.9% of control at 10 minutes, 19.8% at 30 minutes, and 15.5% at 60 minutes, and ADP+AMP were decreased to 70.3% of control at 10 minutes, 67.3% at 30 minutes, and to 27.0% at 60 minutes, but hypoxanthine and xanthine were increased to 121.5% and 127.1% at 10 minutes, 126.0% and 174.4% at 30 minutes, and 141.4% and 197.3% at 60 minutes Total adenosine nucleotides were decreased to 20.3% of control during 60 minutes of ischemia, but hypoxanthine and xathine were increased to 157.5 % of control. These results suggest that the changes in the concentration of nucleotides and their metabolic derivatives are useful indices of the extents of tissue ischemia in rat kidney.

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Water Requirement of Potato According to Growth Stage (노지재배 감자의 생육시기별 물 요구량 구명)

  • Eom, Ki-Cheol;Park, So-Hyun;Yoo, Sung-Yung
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.6
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    • pp.861-866
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    • 2012
  • Water is the most important resource for the potato cultivation, especially to get the maximum water use efficiency and yield of potato, Water has to be applied moderately based on the water requirement of the potato. Crop water requirement (WR) is a function of the Potential evapo-transpiration(PET) and Crop coefficient (Kc). PET can be estimated by the climate data measured at the weather station in the production region. Kc was measured by the NIAST (RDA) through Lysimeter experiments. In this study, the growth stage of potato was divided as four (G-1 : Apr. 1~Apr. 15, G-2 : Apr. 16~May. 10, G-3 : May. 11~May. 31, G4 : Jun. 1~Jun. 15). The average PET during potato growing season of the 45 areas was $2.95mm\;day^{-1}$. The most water requirement was the G-3 stage among the potato growth stage. The MWR (Mean water requirement) according to growth stage was 1.0~1.2 (average 1.1), 1.5~1.8 (average 1.6), 1.9~2.2 (average 2.0) and 1.7~2.1 (average 1.8) mm $day^{-1}$, in the G-1, G-2, G-3 and G-4 stage, respectively. The TWR (Total water requirement) according to growth stage was 18.0~22.1 (average 19.3), 50.6~66.6 (average 56.3), 63.5~88.2 (average 72.4) and 38.3~54.5 (average 44) mm, in the G-1, G-2, G-3 and G-4 stage, respectively.