• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.718-729
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• 2022

Esophageal squamous cell carcinoma (ESCC) is the most common primary esophageal malignancy with poor prognosis. Here, due to the necessity for exploring potential therapies against ESCC, we obtained the gene expression data on ESCC from the TCGA and GEO databases. Venn diagram analysis was applied to identify common targets. The protein-protein interaction network was constructed by Cytoscape software, and the hub targets were extracted from the network via cytoHubba. The potential hub nodes as drug targets were found by pharmacophore-based virtual screening and molecular modeling, and the antitumor activity was evaluated through in vitro studies. A total of 364 differentially expressed genes (DEGs) in ESCC were identified. Pathway enrichment analyses suggested that most DEGs were mainly involved in the cell cycle. Three hub targets were retrieved, including CENPF, CCNA2 (cyclin A), and CCNB1 (cyclin B1), which were highly expressed in esophageal cancer and associated with prognosis. Moreover, amentoflavone, a promising drug candidate found by pharmacophore-based virtual screening, showed antiproliferative and proapoptotic effects and induced G1 in esophageal squamous carcinoma cells. Taken together, our findings suggested that amentoflavone could be a potential cell cycle inhibitor targeting cyclin B1, and is therefore expected to serve as a great therapeutic agent for treating esophageal squamous cell carcinoma.

• International Journal of Automotive Technology
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• 제7권6호
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• pp.729-739
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• 2006

This paper describes an active fault-tolerant control system for an induction motor drive that propels an Electrical Vehicle(EV) or a Hybrid one(HEV). The proposed system adaptively reorganizes itself in the event of sensor loss or sensor recovery to sustain the best control performance given the complement of remaining sensors. Moreover, the developed system takes into account the controller transition smoothness in terms of speed and torque transients. In this paper which is the sequel of (Diallo et al., 2004), we propose to introduce more advanced and intelligent control techniques to improve the global performance of the fault-tolerant drive for automotive applications(e.g. EVs or HEVs). In fact, two control techniques are chosen to illustrate the consistency of the proposed approach: sliding mode for encoder-based control; and fuzzy logics for sensorless control. Moreover, the system control reorganization is now managed by a fuzzy decision system to improve the transitions smoothness. Simulations tests, in terms of speed and torque responses, have been carried out on a 4-kW induction motor drive to evaluate the consistency and the performance of the proposed fault-tolerant control approach.

• BMB Reports
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• 제44권11호
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• pp.725-729
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• 2011

We report a novel mechanism of a CDH1 splicing mutation in a patient with signet ring cell carcinoma of the stomach. A 27-year-old man complaining of aggravated dyspepsia was diagnosed with signet ring cell carcinoma. Both his father and uncle had died of stomach cancer at a young age. DNA sequencing analysis of the CDH1 gene revealed a splice site mutation (c.833-2A>G). By RNA/cDNA sequencing analysis, CDH1 c.833-2A>G generated a new acceptor site within intron 6, causing the insertion of a 79-bp intronic sequence between exon 6 and 7 (r.833-79_833-1ins), and resulting in a frame shift. E-cadherin immunohistochemical staining revealed a loss of CDH1 expression. This study reveals the disease-causing mechanism of this splicing mutation, and emphasizes the need for functional studies using RNA samples for the accurate interpretation of detected splicing variant. This is the first reported case of a CDH1 mutation in a Korean patient.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.725-729
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• 2008

An enzyme reactor installed with ultrafiltration membrane was developed to produce ${\alpha}-,\;{\beta}-$, and ${\gamma}$-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTKIOase). Ultrafiltration membrane YM10 with 10,000 of molecular cutoff was chosen for membrane modification and CD production. A repeated-batch type of the enzyme reaction with free CGTK10ase resulted in a ${\alpha}$-CD yield of 24.0 (${\pm}1.5$)% and a productivity of 4.68 (${\pm}0.88$) g/l-h, which were 7 times higher that those for CGTK10ase immobilized on modified YM10 membrane. Addition of 1-nonanol increased CD yields by 30% relative to the control, which might be due to prevention of the reversible hydrolysis of CDs.

• 천문학회보
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• 제38권2호
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• pp.48.2-48.2
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• 2013

To understand the connection between active galactic nuclei (AGNs) and star formation, we investigated the relation between AGN bolometric and far-infrared (FIR) luminosities, using type-2 AGNs. By matching type-2 AGNs at z < 0.3 selected from the SDSS based on the emission-line diagnostics, against the AKARI/FIS All-Sky Survey Catalogue and the COSMOS PEP (PACS Evolutionary Probe) Survey Catalogue, we obtained a sample of 729 type-2 AGNs detected in the AKARI survey ($90{\mu}m$) and 17 ones detected in the PEP survey ($100{\mu}m$). For AGN bolometric luminosities, we adopted an estimate based on the [OIII] and [OI] line luminosities. We confirmed that there is a correlation between the AGN bolometric and FIR luminosities with a large scatter, which is consistent with previous studies. However, we claim that this correlation suffers from various artificial effects, e.g., FIR detection limits, survey volumes, and so on. We will discuss the limitations of studying the connection between AGN and star formation using currently available facilities.

• 한국재료학회지
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• 제15권11호
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• pp.722-729
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• 2005

The electrical and dielectric stability of zinc oxide-based varistors were investigated with the cobalt oxide contents in the range of $0.5\~5.0 mo\l%$. As cobalt oxide contents increased, the ceramic density increased in the range of $5.25\~5.55g/cm^3$ and the varistor voltage decreased in the range of $235.3\~86.0V$. The varistor with on addition of cobalt oxide $1.0 mol\%$ exhibited good nonlinearity. in which the nonlinear exponent is 66.6 and the leakage current is $1.2{\mu}A$. Furthermore, the varistors exhibited the highest electrical and dielectric stability, with $\%{\Delta}V_{1mA}=-1.9\%,\;\%{\Delta}{\alpha}=-10.5\%,\;\%{\Delta}I_L=+275.0\%,\;and\;\%{\Delta}tna{\delta}=+55.6\%$, under DC accelerated aging $0.95V_{1mA}/150^{\circ}C/24h$.

• 한국통신학회논문지
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• 제38B권4호
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• pp.279-290
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• 2013

본 논문에서는 UWB의 광대역 주파수와 낮은 신호세기의 특성을 이용해서 기밀한 음성 통신이 가능하고, 이동 ad hoc 네트워크로 동작하도록 하여 단말의 이동이 가능한 전술망용 MAC 프로토콜의 효과적인 음성 데이터 전송 기법을 제안하였다. 기존의 UWB MAC 프로토콜은 대부분 단말 간 동기화를 지원하여 전력 소모, 대역폭과 같은 자원 활용에 중점을 두었으나, 본 논문의 UWB MAC 프로토콜은 단말의 이동성에 중점을 두어 비동기 방식으로 구동된다. 또한 효과적인 음성 데이터 전송을 위해서 RTS/CTS 패킷을 사용하지 않고, 재전송 과정이 없다. 해당 음성 데이터 전송 방식의 시뮬레이션을 통하여 성능을 측정하였다.

• 한국환경과학회지
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• 제17권5호
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• pp.501-507
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• 2008

The concentrations of HCHO(formaldehyde), $PM_{10}$(particulate matter), $CO_2$(carbon dioxide) and TBC(total bacteria counter) distribution in schools(Chung-Nam Area) were examined, and the results were compared with the recommended criterion of the administration law of indoor air. The subjects were an elementary school, a middle school and a high school in Chung-Nam area, and the concentration of TBC was examined by Single Stage Air Cascade Sampler, which applied the inertia collision catching method of 28.29L/min(flux) during 5 months from March, 2007 to July, 2007. The instrument(LD-3B, SIBATA Company)was used to examine $PM_{10}$, by a light scattering method and a light transmission method. The instrument(Airboxx(KD Engineering) was used to examine $CO_2$. The instrument(Z300XP(Environmental sensor)was used to examine HCHO. The result indicated that the $PM_{10}$ average concentrations of the surveyed classrooms were $49{\mu}g/m^3$ in Spring and $59{\mu}g/m^3$ in Summer. The $CO_2$ average concentration of the surveyed schools were 576 ppm in the classroom and 527 ppm in the stateroom. The average concentration of TBC were $729CFU/m^3$ in an elementary school, $401CFU/m^3$ in a middle school, $381CFU/m^3$ in a high school. The HCHO average concentration of the surveyed schools were 0.03 ppm in the classroom, 0.02 ppm in the stateroom.

• 한국토양비료학회지
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• 제17권2호
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• pp.173-178
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• 1984

옥수수 수량(收量)과 토양(土壤)의 화학적특성(化學的特性)과의 상관관계(相關關係)를 검토(檢討)하여 옥수수 재배지토양(栽培地土壤)의 토양화학성(土壤化學性) 개량(改良) 기준(基準)을 설정(設定)할 목적(目的)으로 16개(個) 옥수수 시범재배포장(示範栽培圃場)을 대상(對象)으로 조사(調査)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 옥수수 수량(收量)과 토양화학성(土壤化學性)과의 상관관계(相關關係)에서 $pH\;r=0.706^{**}$, $Av.\;P_2O_5\;r=0.675^{**}$, $Ex.\;K\;r=0.697^{**}$, $Ex.\;Ca\;r=0.763^{**}$, $Ex.\;Mg\;r=0.626^{**}$, $CEC\;r=0.803^{**}$, $Av\;SiO_2\;r=0.670^{**}$, $B.S\;r=0.729^{**}$로 정(正)의 상관관계(相關關係)를 나타내었으나 유기물(有機物) 및 전질소함량(全窒素含量)과는 유의성(有意性)이 인정(認定)되지 않았다. 2. 옥수수에 대한 토양중(土壤中) 적정무잔성함량(適正無棧成含量)(1,000kg/10a생산기준(生産基準))은 pH5.6, Av $P_2O_5$, 327ppm, Ex. K0.39me/100g, Ex. Ca 5.5me/100g, Ex. Mg1.3me/100g, Ex. Al 0.44me/100g, CEC 11.5me/100g, Av. $SiO_2$, 116ppm. B.S 58%로 추정(准定)되었다. 3. 토양중(土壤中) 치환성(置換性)알루미늄함량(含量)은 옥수수 수량(收量)뿐만 아니라 토양산도(土壤酸度), 유효인산(有效燐酸), 치환성가리(置換性加理), 치환성석회함량(置換性石灰含量)과도 유의성(有意性)있는 부(負)의 상관관계(相關關係)를 나타내었다.

• Bulletin of the Korean Chemical Society
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• 제26권5호
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• pp.729-734
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• 2005

A sensitive and selective method for quantitation of sofalcone and its active metabolite in human plasma has been established using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Plasma samples were transferred into 96-well plate using an automated sample handling system and spiked with 10 $\mu$L of 2 $\mu$g/mL $d_3$-sofalcone and $d_3$-sofalcone metabolite solutions (internal standard), respectively. After adding 0.5 mL of acetonitrile to the 96-well plate, the plasma samples were then vortexed for 30 sec. After centrifugation, the supernatant was transferred into another 96-well plate and completely evaporated at 40 ${^{\circ}C}$ under a stream of nitrogen. Dry residues were reconstituted with mobile phase and were injected into a $C_{18}$ reversed-phase column. The limit of quantitation of sofalcone and its metabolite was 2 ng/mL, using a sample volume of 0.2 mL for analysis. The reproducibility of the method was evaluated by analyzing 10 replicates over the concentration range of 2 ng/mL to 1000 ng/mL. The validation experiments of the method have shown that the assay has good precision and accuracy. Sofalcone and its metabolite produced a protonated precursor ion ([M+H]$^+$) of m/z 451 and 453, and a corresponding product ion of m/z 315 and 317, respectively. Internal standard ($d_3$-sofalcone and $d_3$-sofalcone metabolite) produced a protonated precursor ion ([M+H]$^+$) of m/z 454 and 456 and a corresponding product ion of m/z 315 and 317, respectively. The method has been successfully applied to a pharmacokinetic study of sofalcone and its active metabolite in human plasma.