• Title/Summary/Keyword: G. Mendel

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Gregor Mendel and the Seven Genes (1)

  • Tateno, Yoshio
    • Interdisciplinary Bio Central
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    • v.5 no.4
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    • pp.7.1-7.3
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    • 2013
  • This essay describes G. Mendel's life and his law of inheritance. He was born in a poor family in 1822 in a hamlet in Czechs. At that time the Habsburg Empire dominated over the East Europe in which Vienna was the capital. Vienna had thus been the center of culture and learning, and attracted many artists and scholars such as W. Mozart (1756- 1791), L. Beethoven (1770-1827), C. Doppler (1803-1853), S. Freud (1856-1939), G. Mahler (1860-1911), G. Klimt (1862-1918) and E. Schiele (1890-1918). Beethoven came to Vienna to learn from Mozart. Klimt was influence by Schilele. When Mahler consulted Freud about his mental problem, Freud said to him "Your mental condition was not normal, but the condition made you creative. So, do not worry too much about it." Like that, there were many interactions among them, and Mendel was no exception. Though Mendel was poor, he was fortunate in his education and scientific research, because he could have excellent supporters in his family and out of it. He learned mathematics and physics at Vienna University under the guidance of C. Doppler. He was not totally alone when he discovered his law of inheritance. It may not be true either that his law was neglected and rediscovered in the year of 1900. As his one and only paper indicates, he was one of the earliest interdisciplinary scientists.

Gregor Mendel and the Seven Genes (2)

  • Tateno, Yoshio
    • Interdisciplinary Bio Central
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    • v.5 no.4
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    • pp.8.1-8.3
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    • 2013
  • Unlike the traditional view, it is not mysterious about how G. Mendel chose the seven characters of the pea, Pisum sativum, that he studied. He first chose the pea that met three conditions he set up and repeated experiments for two years. Apparently, he knew that those characters were controlled by countable elements. Then, he derived the prediction on the basis of his idea about the elements, and selected the seven characters that satisfied the prediction. He knew "no prediction no science". In population genetics the Hardy-Weinberg principle is well known and cited in many papers and books. However, Mendel already derived the same principle in his paper, because he was acquainted also with physics and mathematics. Actually, the principle was trivial when they derived, but not at all when Mendel did. It is also well known that Mendel's laws were forgotten and rediscovered at the term of the 19th century. That may not be true either. His laws were internationally well known before the rediscovery. In fact, the 1881-year version of the Encyclopedia Britannica contains his laws.

Development of Information Biology (II)

  • Tateno, Yoshio
    • Interdisciplinary Bio Central
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    • v.5 no.2
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    • pp.4.1-4.3
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    • 2013
  • A history of discoveries of a gene and DNA was viewed with respect to people, time and places. It started with G. Mendel and J. Meisher, who discovered a gene in a plant species in 1866 and DNA in animals in 1869, respectively. With recognition that DNA was a chemical substance, A. Kossel identified the four chemical components of DNA without knowing their biological function around the turn of the 19th century. On the other hand F. Griffith found a peculiar activity in a bacterial species in 1928, but victimized by the war before understanding what it was. Those discoveries were made in Europe, but they were still fragmentary. Then, in USA, O. T. Avery, A. Hershey, M. Nirenberg and other scientists organized the European discoveries and elucidated their coordinated biological functions in 1950's and 1960'.

Analysis of the Foreign Gene Transmission in the GFP Transgenic Chickens (형질전환 닭에서 GFP 유전자 전이 연구)

  • Jang, Ye-Jin;Ji, Mi-Ran;Jeon, Mi-Hyang;Kim, Jeom-Sun;Kim, Kyung-Woon;Han, Deug-Woo;Chung, Hak-Jae;Yang, Byoung-Chul;Yoo, Jae-Gyu;Park, Jin-Ki;Kim, Te-Oan;Byun, Sung-June
    • Korean Journal of Poultry Science
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    • v.39 no.3
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    • pp.241-244
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    • 2012
  • This study was performed to analyze the generational transmission and the expression of the foreign gene in the GFP transgenic chickens. The transmission rate and the expression of the GFP gene was investigated from the GFP transgenic rooster (G2) as the first founder to the ninth (G8). Analysis of GFP expression in hatched chickens was used the UV lamp. When GFP was expressed in the wings, bill and legs of a chick, the bird only was selected as a transgenic chick. The average transmission rate of the overall transgenic was 38~58%. These results showed that the transmission of the GFP gene in the transgenic chickens in accordance with the laws of Mendel's continues to the next generation without gene silencing.

Development and Utilization of KASP Markers Targeting the Lipoxygenase Gene in Soybean

  • Seo-Young Shin;Se-Hee Kang;Byeong Hee Kang;Sreeparna Chowdhury;Won-Ho Lee;Jeong-Dong Lee;Sungwoo Lee;Yu-Mi Choi;Bo-Keun Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.4
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    • pp.294-303
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    • 2023
  • Lipoxygenase gives soybeans their grassy flavor, which can disrupt food processing efficiency. This study aimed to identify soybean genotypes with lipoxygenase deficiency among 1,001 soybean accessions and to develop kompetitive allele specific PCR (KASP) markers that can detect lipoxygenase mutations. Three lipoxygenase isozymes (Lox1, Lox2, and Lox3) were analyzed using a colorimetric assay based on a substrate-enzyme reaction. Among the 1,001 accessions examined, two (IT160160 and IT276392) exhibited a deficiency solely in Lox1, and one (IT269984) lacked both Lox1 and Lox2. IT160160 had a 74-bp deletion in exon 8 of Lox1 (Glyma13g347600), whereas IT276392 displayed a missense mutation involving the change of C to A at position 2,880 of Lox1. Moreover, we successfully developed four KASP markers that specifically target Lox1, Lox2, and Lox3 mutations. To validate the Lox1 KASP markers, we used two F2:3 populations generated through a cross between Daepung 2 (lipoxygenase wild type, maternal parent), IT160160, and IT276392 (null Lox1, paternal parent). The results revealed that the Daepung 2 × IT160160 group followed the expected 3:1 ratio according to Mendel's law, whereas the Daepung 2 × IT276392 group did not. Furthermore, a comparison between the colorimetric and KASP marker analyses results revealed a high agreement rate of 96%. KASP markers offer a distinct advantage by allowing the distinction of heterozygous types independent of other variables. As a result, we present an opportunity to expedite the lipoxygenase-deficient cultivar development.

An Analysis on Problem-Finding Patterns of Well-Known Creative Scientists (잘 알려진 창의적 과학자들의 과학적 문제 발견 패턴 분석)

  • Kim, Youngmin;Seo, Hae-Ae;Park, Jongseok
    • Journal of The Korean Association For Science Education
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    • v.33 no.7
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    • pp.1285-1299
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    • 2013
  • Nurturing students' scientific creativity is considered an important element in science education in Korea. The study aims to explore patterns displayed by well-known scientists in their quest for problem finding. Each case of scientists' course of problem solving is described in terms of historical background, a process of problem finding, and a process of problem solving. There are five patterns from ten scientists which are as follows: Pattern 1 is that scientists find problems from insufficiencies and/or errors from explanation of theories at the time and the related cases are A. Lavoisier, G. Mendel, and J. Watson. Pattern 2 shows that scientists find a problem because of strange phenomena unexplained by theories at the time, and here important case studies are E. Rutherford and W. R$\ddot{o}$ntgen. Pattern 3 demonstrates that scientists find a problem from analogical reasoning between known theories and unknown science phenomena. The cases include S. Carnot and T. Young. Pattern 4 points to the fact that scientists find a problem while they utilize a newly invented experimental instrument. Here, G. Galilei is an important example. Pattern 5 establishes that scientists happen to find a problem while they conduct research projects. The works of M. Faraday and J. Kepler are prominent case studies related to this pattern.

Studies on the Breeding of the Response to short photoperiod, Fiber weight, and Qualitative characters and of the Associations Among these characters in Kenaf (섬유용양마의 육종에 관한 연구 -단일반응성과 섬유종의 유전 및 연소)

  • Johng-Moon Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.115-124
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    • 1968
  • It was shown that the most desirable characters for kenaf are high-fiber weight and moderately early maturity. Therefore, the objectives of this research on this crop is to find varieties possessing these characteristics. The experiments covered in this report provided new information relative to segregation, mode of inheritance, estimate of the number of genes involved in fiber weight and their response to short day length of 10 hours and the qualitative characters, such as, color of stem, capsule, petiole and shape of leaves. The associations which exist among these characters are also indicated. Fiber weight per plant, days to flowering, Stem color, Petiole color, Capsule color, and shape of leaves were studied in parental, $F_1$.$F_2$and backcross populations of a cross between Dashkent, a low-fiber weight but early maturing kenaf variety, and G 38 F-1, a high-fiber weight but late maturing kenaf variety. Crosses were made using the varieties, Dashkent and G 38 F-1 as parents. The Dashkent parent had the following characteristics: green stems, capsules and petioles and lobed shaped leaves; 105.8234 mean-days to flowering in the field, and 106.9222 mean-days under 10 hours short day treatment. The other parent, G 38 F-1 had red stems yellow capsules and red petioles and unlobed shaped leaves; 149.8921 mean-days to flowering in the field, and 62.3684 mean-days under 10 hours short day treatment. Both of the parents, $F_1$, $F_2$, $BC_1$ ($F_1$ X Dashkent, ) and $BC_2$($F_1$ ${\times}$ G38F-1) of the kenaf cross were grown at the Crops Experiment Station, Suwon, Korea in 1965. Color of stems, petioles and capsules, and shape of leaves were noted to be simply inherited as a single factor. Red stem color was dominant over green stem color, red petiole color was dominant over green petiole, lobed shaped leaves were dominant over unlobed shaped leaves and yellow capsules were dominant over green capsule. It was, also, noted that the factor for color of petiole was linked with the factor for shape of leaf with a 11.9587 percent recombination value, however no interaction or linkage were found among the color of stem and capsule color. Using Powers partitioning method, theoretical means and frequency distributions for each population, the days to flowering were calculated with the assumption that two gene pairs were involved. The values obtained fitted the theoretical values. In general this would indicate that Dashkent and G 38 F -1 were differentiated by two gene pairs. Heritability values were calculated as the percent of additive genetic variance. Heritability value of days to flowering, 89.5% in the broad sense and 79.91% in the narrow sense, indicated that the selection for this character would be effective in relatively early generations. Particularly, high positive correlations were found between days to flowering and the color of petioles and shape of leaves. However, there was no relation between days to flowering and capsule color nor between these and stem color. On the basis of the results of this experiment there is evidence that the hereditary factor for shape of leaves and the color of petioles is linked with an effective factor or factors for the characters of days to flowering. The association was sufficiently close to offer a possible simple and efficient means of selection for moderately early mat. uring plants by leaf shape and petiole color selection. Again using Powers partitioning method the frequency distribution for each population to the fiber weight were calculated with the assumption that two gene pairs, AaBb, were involved. Both phenotypic and genotypic dominance were complete. The obtained value did not agree with the theoretical value for $F_2$ and $BC_1$ ($F_1$ ${\times}$ Dashkent.) It seems that Dashkent and G 38 F-1 were differentiated by two major gene pairs but some the other minor genes are necessary. It is certain that the hereditary factor for shape of leaves and color of petioles is linked with an effective factor or factors for fiber weight. Also, high. yielding plants with moderately early maturity were found in the $F_2$ population. Thus, simultaneous selection for high-fiber yield and moderately early maturing plants should be possible in these populations. Phenotypic and genotypic correlation coefficients between fiber weight per plant and days to flowering, stem height and stem diameter were calculated. In general, genotypic correlations are higher than the phenotypic correlation. The highest correlation is found between stem height and fiber weight per plant (0.7852 in genotypic and 0.4103 in phenotypic) and between days to flowering and fiber weight per plant (0.7398 in genotypic and 0.3983 in phenotypic.) It was also expected that the selection of high stem height and moderately early maturing plants were given the efficient means of selection for high fiber weight.

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