• Journal of Ginseng Research
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• v.1 no.1
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• pp.33-50
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• 1976

As a continuation of series of works on the pharmacological actions of Panax ginseng. three kinds of behavioral experiments were carried out using rats and mice. The occurrence of component Posterns of general behavioral activity in rat was examined by visual scanning using the ting sample method in the ad lib. And he hunger deprivated situation. In normal ad lib. situation, the eating behavior of rat treated with 100mg/kg of ginseng saponin was significantly more frequent than that of saline control at the night and throughout the 24 hr period. But grooming was less frequent than the control at the same period. In the hunger situation followed by 90~120 hrs of feed deprivation, the locomotive activity and rearing awe significantly more often and sleeping was less frequent in the two dosage g roups of ginseng saponin (10 and 100 mg/kg) than in the saline group though out the observation period. Training of avoidance conditioning in rats was done in a two-way shuttle box. The number of conditioned response (CR) in which the animal avoided sucessfully an electric shock by running in to the other compartment of the hex was regarded as an index of learning performance. Ginseng saponin in doses of 2.5 mg/kg Produced a significantly increased CR in total avoidance tria1s compared with the control. Although other dosage groups of ginseng saponin (5.0, 50mg and 100 mg/kg) showed no significant statistical difference from the normal control, it tended to increase in CR in the ginseng groups than in the control. An aggressive behavior in mice was observed in n shock-generating fighting box. The occurrence of reflexive fighting between two animals induced by an electric shock applied to the feet war checked as an index of aggression. The occurrence of reciprocal fighting episode immediately after the onset. Of the shock was significantly decreased in the dosage group of 400 mg/kg ginseng saponin, but it did net differ in the 100 mg/kg group of ginseng saponin from the control group. The dose, 400 mg/kg of ginseng saponin, inhibited fighting behavior in more than 80% of the Pairs. but 100 mg/kg of ginseng did inhibit it in less than 20% of the pairs.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.1930-1936
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• 2007

A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencing CyA production. Among the 11 variables tested, sucrose, ammonium sulfate, and soluble starch were selected, owing to their significant positive effect on CyA production. A response surface methodology (RSM) involving a 3-level Box-Behnken design was adopted to acquire the best process conditions. Thus, a polynomial model was created to correlate the relationship between the three variables and the CyA yield, and the optimal combination of the major media constituents for cyclosporin A production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows (g/l): sucrose, 20; starch, 20; and ammonium sulfate, 10. The predicted optimum CyA yield was 113 mg/l, which was 2-fold the amount obtained with the basal medium. Experimental verification of the predicted model resulted in a CyA yield of 110 mg/l, representing 97% of the theoretically calculated yield.

• International Journal of Industrial Entomology and Biomaterials
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• v.12 no.2
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• pp.57-61
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• 2006

Suppressor of cytokine signaling (SOCS) is known to be as a negative feedback regulator in Janus kinase signal transducer and activator of transcription signaling. Highly conserved SOCS box domain was cloned from a Korean malaria vector, Anopheles sinensis. Sequence analysis indicates that it has identity to Anopheles gambiae (96%), Aedes aegypti (94%), Drosophila melanogaster (78%), Mus musculus (72%) and Homo sapiens (72%), respectively. Tissue specificity RT-PCR demonstrated that the expression level of AsSOCS transcript was high at abdomen, midgut, and ovary, whereas developmental expression patterns showed that the level of AsSOCS was high at egg, early pupae, and adult female. On the other hand, RT-PCR analysis after bacterial challenge showed that SOCS mRNA was strongly induced in larvae. In addition, it was also induced by various immune elicitors such as lipoteicoic acid, CpG-DNA, and laminarin. It seems that AsSOCS, repressor of JAK-STAT pathway, is highly conserved in mosquito, and may play an important role in mosquito innate immune response.

• Journal of Microbiology
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• v.44 no.6
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• pp.641-648
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• 2006

We previously reported that Skp1, a component of the Skp1-Cullin-F-box protein (SCF) complex essential for the timely degradation of cell cycle proteins by ubiquitination, physically interacts with Bfa1, which is a key negative regulator of the mitotic exit network (MEN) in response to diverse checkpoint-activating stresses in budding yeast. In this study, we initially investigated whether the interaction of Skp1 and Bfa1 is involved in the regulation of the Bfa1 protein level during the cell cycle, especially by mediating its degradation. However, the profile of the Bfa1 protein did not change during the cell cycle in skp1-11, which is a SKP1 mutant allele in which the function of Skp1 as a part of SCF is completely impaired, thus indicating that Skp1 does not affect the degradation of Bfa1. On the other hand, we found that the skp1-12 mutant allele, previously reported to block G2-M transition, showed defects in mitotic exit and cytokinesis. The skp1-12 mutant allele also revealed a specific genetic interaction with ${\Delta}bfa1$. Bfa1 interacted with Skp1 via its 184 C-terminal residues (Bfa1-D8) that are responsible for its function in mitotic exit. In addition, the interaction between Bfa1 and the Skp1-12 mutant protein was stronger than that of Bfa1 and the wild type Skp1. We suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex. The interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit.

• Journal of Ecology and Environment
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• v.42 no.2
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• pp.85-89
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• 2018

Observations and video documentation of interactions between animals living in dens, cavities, and other enclosed spaces are difficult, but they play an important role in field biology, ecology, and conservation. For example, bird parents visiting nests and feeding their nestlings may provide crucial information for testing of ecological hypotheses and may easily attract attention of participants of citizen-science ecological and conservation projects. Because of the nest concealment of cavity-nesting birds, their behaviors in the nest can only be studied by using video surveillance. Professional wildlife surveillance systems are extremely expensive. Here, we describe an inexpensive video setup that can be constructed with relatively little effort and is more affordable than any previously described system. We anticipate that the relatively low cost of about 250 USD for a battery-operated system is an important feature for citizen-science type of projects and for applications in heavily populated areas where the potential for theft and vandalism may be high. Based on our experiences, we provide methodological advice on practical aspects of using this system in the field for ecological research on birds. We highlight the low cost, easiness of construction, and potential availability to a large number of observers taking part in wildlife monitoring projects, and we offer technical help to participants of such research projects.

• Steel and Composite Structures
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• v.24 no.2
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• pp.227-237
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• 2017

This paper presents optimization of a long-span portal steel frame under dynamic wind loads using a surrogate-assisted evolutionary algorithm. Long-span portal steel frames are often used in low-rise industrial and commercial buildings. The structure needs be able to resist the wind loads, and at the same time it should be as light as possible in order to be cost-effective. In this work, numerical model of a portal steel frame is constructed using structural analysis program (SAP2000), with the web-heights at five locations of I-sections of the columns and rafters as the decision variables. In order to evaluate the performance of a given design under dynamic wind loading, the equivalent static wind load (ESWL) is obtained from a database of wind pressures measured in wind tunnel tests. A modified formulation of the problem compared to the one available in the literature is also presented, considering additional design constraints for practicality. Evolutionary algorithms (EA) are often used to solve such non-linear, black-box problems, but when each design evaluation is computationally expensive (e.g., in this case a SAP2000 simulation), the time taken for optimization using EAs becomes untenable. To overcome this challenge, we employ a surrogate-assisted evolutionary algorithm (SAEA) to expedite the convergence towards the optimum design. The presented SAEA uses multiple spatially distributed surrogate models to approximate the simulations more accurately in lieu of commonly used single global surrogate models. Through rigorous numerical experiments, improvements in results and time savings obtained using SAEA over EA are demonstrated.

• Journal of the Korea Institute of Military Science and Technology
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• v.20 no.5
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• pp.688-699
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• 2017

Model-based test, a well-known method of the black box tests, is consisted of the following four steps : model construction using requirement, test case generation from the model, execution of a SUT (software under test) and detection failures. Among models constructed in the first step, state-based models such as UML standard State Machine are commonly used to design event-based embedded systems (e.g., weapon control systems). To generate test cases from state-based models in the next step, coverage-based techniques such as state coverage and transition coverage are used. Round-trip path coverage technique using W-Method, one of coverage-based techniques, is known as more effective method than others. However it has a limitation of low failure observability because the W-Method technique terminates a testing process when arrivals meet states already visited and it is hard to decide the current state is completely same or not with the previous in the case like the GUI environment. In other words, there can exist unrevealed faults. Therefore, this study suggests a Extended W-Method. The Extended W-Method extends the round-trip path to a final state to improve failure observability. In this paper, we compare effectiveness and efficiency with requirement-item-based technique, W-Method and our Extended W-Method. The result shows that our technique can detect five and two more faults respectively and has the performance of 28 % and 42 % higher failure detection probability than the requirement-item-based and W-Method techniques, respectively.

• Proceedings of the KIEE Conference
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• 2004.11c
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• pp.532-534
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• 2004

The advancement of electronics and telecommunication technologies has forced the risk management system for underground metallic structures to evolve into the remote monitoring and control system. Especially, facilities such as gas pipelines, oil pipelines and water distribution lines might make hazardous effect on human safety without continuous monitoring and control. As a result, pipeline engineers have applied cathodic protection system to prevent the degradation of their facilities by corrosion and carried out a periodic monitoring of the pipe-to-soil (P/S) potentials at numberous test boxes along their pipelines. The latter action on a road in downtowns, however, is so much dangerous that the inspectors should be ready to suffer the threatening of their lives and maintenance. In order to minimize these social costs and hazards, a stand-alone type corrosion monitoring equipment which can be installed in test box, store the P/S data for given Belied and send the data by wired/wireless telecommunications is under development. In order to obtain the exact P/S data, however, a reference electrode should be located as close to the pipeline as possible. Actually, the measured potential by a conventional portable reference electrode contain inevitably an IR drop portion caused by the current flow from the cathodic protection rectifier or the subway railroad. To minimize this error, it is recommended that the reference electrode should be buried within 10 cm from the pipeline. In this paper, we describe the design parameters for fabricating the permanent type reference electrode and the characteristics of the developed reference electrode.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.18 no.1
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• pp.39-48
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• 2012

Background: This study was performed to identify what effect plyometric exercise with application of kinesio taping gives on muscle activity and power of lower limb. Methods: In order to conduct research, total 14 male students at G university located Gwangyang city were randomly assigned; 7 for experimental group (with kinesio taping application) and 7 for control groups(without kinesio taping application) were conducted with box drill, one of the plyometric exercise 5 times a week for 4 weeks. Muscle activities were measured by using electrodiagnosis equipment on quadriceps and gastrocnemius. Collected data was analyzed by using Windows SPSS version 19.0. Results: Muscle activities show statistically significant differences (p<.05) both before and after exercise in experimental and control groups in terms of vastus medialis, rectus femoris, vastus lateralis and lateral gastrocnemius. However, only medial gastrocnemius shows statistically significant differences in experimental group compared before and after exercise. Sargent jump for measuring power shows that only experimental group presents statistically significant differences (p<.05). Two groups show that vastus medialis, vastus lateralis, lateral gastrocnemius and medial gastrocnemius only in muscle activities show statistically significant differences (p<.05) after exercise. Conclusion: The result of this survey indicates that plyometric exercise is effective to improve muscle activity and power of lower limb, especially, the group who exercises with application of kinesio tape is more effective than the other group with no tape application. Therefore, it is considered that plyometric exercise with tape is more effective to improve power and muscle strength.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.47-51
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• 2004

To evolve silkworm hybrids with higher survival and productivity and easy and effective seed cocoon production, three new evolved bivoltine hybrids of silkworm (Bombyx mori L.), created with participation of sex-limited lines at eggs and larva stage were evaluated with control Super$_1$${\times}$Hessa$_2$ hybrid at Plovdiv Agrarian University during 2000 - 2002. ANOVA analysis was employed and the performance of hybrids was observed in respect of main quantitative traits. The results obtained show significant superiority of new hybrids T$_{15}$ 4/${\times}$TBV$_{2}$24/, XT$_{215}$38/${\times}$TV$_{3}$2/, XT$_{215}$38/${\times}$B$_{2}$6/ and their reciprocal crosses over the control. They were characterized with 99% hatchability of eggs, 93-95% silkworm survival, 2.32- 2.42 g cocoon weight, 53.5-55.7 cg shell weight, 22.9-23.1% shell ratio, 1,307-1,326 m filament length, 3.08-3.17 denier, 95-96% reelability, 44.4-45.1% silk ratio, 569-593 number of normal eggs per lying and 347-364 mg weight of normal eggs per lying. Newly evolved hybrids were manifested high productivity, 41.0-43.6 kg cocoon yield and 7.33-7.78 kg raw silk yield per one box (20,000$\pm$200 viable eggs), which significant surpass the control Super$_1$${\times}$Hessa$_2$ hybrid with 7.6-14.4% and 10.8-17.6%, respectively. T$_{15}$ 4/${\times}$TBV$_{2}$24/ and XT$_{215}$38/${\times}$TV$_{3}$2/ were considered as highly productive hybrids to local conditions and found suitable to rear in spring season.n.