International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Induction Patterns of Suppressor of Cytokine Signaling (SOCS) by Immune Elicitors in Anopheles sinensis

  • Noh Mi-Young (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University) ;
  • Jo Yong-Hun (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee Yong-Seok (Department of Parasitology, Inje University of Medical School) ;
  • Kim Heung-Chul (US Army, 5th Medical Detachment, 18th Medical Command) ;
  • Bang In-Seok (MyGene Bioscience Institute) ;
  • Chun Jae-Sun (Department of Biological Education, Korea National University of Education) ;
  • Lee In-Hee (Department of Bio-Technology, Hoseo University) ;
  • Seo Sook-Jae (Division of Applied Life Science, Gyeongsang National University) ;
  • Shin E-Hyun (Korean National Institute of Health, Department of Viral Diseases) ;
  • Han Man-Deuk (Department of Dental Hygiene, Kimcheon College) ;
  • Kim Ik-Soo (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University) ;
  • Han Yeon-Soo (Department of Agricultural Biology, College of Agriculture and Life Science, Chonnam National University)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Suppressor of cytokine signaling (SOCS) is known to be as a negative feedback regulator in Janus kinase signal transducer and activator of transcription signaling. Highly conserved SOCS box domain was cloned from a Korean malaria vector, Anopheles sinensis. Sequence analysis indicates that it has identity to Anopheles gambiae (96%), Aedes aegypti (94%), Drosophila melanogaster (78%), Mus musculus (72%) and Homo sapiens (72%), respectively. Tissue specificity RT-PCR demonstrated that the expression level of AsSOCS transcript was high at abdomen, midgut, and ovary, whereas developmental expression patterns showed that the level of AsSOCS was high at egg, early pupae, and adult female. On the other hand, RT-PCR analysis after bacterial challenge showed that SOCS mRNA was strongly induced in larvae. In addition, it was also induced by various immune elicitors such as lipoteicoic acid, CpG-DNA, and laminarin. It seems that AsSOCS, repressor of JAK-STAT pathway, is highly conserved in mosquito, and may play an important role in mosquito innate immune response.

Keywords

References

  1. Alexander, W. S. and D. J. Hilton (2004) The role of suppressors of cytokine signaling (SOCS) proteins in regulation of the immune response. Ann. Rev. Immunol. 22, 503-529 https://doi.org/10.1146/annurev.immunol.22.091003.090312
  2. Barillas-Mury, C., Y. S. Han, D. Seeley and F. C. Kafatos (1999) Anopheles gambiae Ag-STAT, a new insect member of the STAT family, is activated in response to bacterial infection. Embo J. 18, 959-967 https://doi.org/10.1093/emboj/18.4.959
  3. Barillas-Mury, C., B. Wizel and Y. S. Han (2000) Mosquito immune responses and malaria transmission: lessons from insect model systems and implications for vertebrate innate immunity and vaccine development. Insect Biochem. Mol. Biol. 30, 429-442 https://doi.org/10.1016/S0965-1748(00)00018-7
  4. Callus, B. A. and B. Mathey-Prevot (2002) SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGFR signaling in the imaginal wing disc. Oncogene. 21, 4812-4821 https://doi.org/10.1038/sj.onc.1205618
  5. Elliott, J. and J. A. Johnston (2004) SOCS: role in inflammation, allergy and homeostasis. Trends Immunol. 25, 434-440 https://doi.org/10.1016/j.it.2004.05.012
  6. Han, Y. S., J. Thompson, F. C. Kafatos and C. Barillas-Mury (2000) Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes. EMBO J. 19, 6030-6040 https://doi.org/10.1093/emboj/19.22.6030
  7. Han, Y. S. and C. Barillas-Mury (2002) Implications of time bomb model of ookinete invasion of midgut cells. Insect Biochem. Mol. Biol. 32, 1311-1316 https://doi.org/10.1016/S0965-1748(02)00093-0
  8. Kamura, T., S. Sato, D. Haque, L. Liu, W. G. Kaelin, R. C. Conaway and J. W. Conaway (1998) The Elongin BC complex interacts with the conserved SOCS-box motif present in members of the SOCS, ras, WD-40 repeat, and ankyrin repeat families. Genes Dev. 12, 3872-3881 https://doi.org/10.1101/gad.12.24.3872
  9. Karsten, P., S. Hader and M. Zeidler (2002) Cloning and expression of Drosophila SOCS36E and its potential regulation by the JAK/STAT pathway. Mech Dev. 117, 343-346 https://doi.org/10.1016/S0925-4773(02)00216-2
  10. Lin, C. C., C, M. Chou, Y. L. Hsu, J. C. Lien, Y. M. Wang, S. T. Chen, S. C. Tsai, P. W. Hsiao and C. J. Huang (2004) Characterization of two mosquito STATs, AaSTAT and CtSTAT. Differential regulation of tyrosine phosphorylation and DNA binding activity by lipopolysaccharide treatment and by Japanese encephalitis virus infection. J. Biol. Chem. 279, 3308-3317 https://doi.org/10.1074/jbc.M309749200
  11. Luckhart, S., Y. Vodovotz, L. Cui and R. Rosenberg (1998) The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide. Proc. Natl. Acad. Sci. USA. 95, 5700-5705
  12. Rawlings, J. S., G. Rennebeck, S. M. Harrison, R. Xi and D. A. Harrison (2004) Two Drosophila suppressors of cytokine signaling (SOCS) differentially regulate JAK and EGFR pathway activities. BMC Cell Biol. 5, 38-52 https://doi.org/10.1186/1471-2121-5-38
  13. Shin, E. H., H. W. Lee, B. Y. Kim, J. H. Lee, H. I. Lee, Y. H. Shin and W. J. Lee (2003) Study on laboratory colonization and mass-rearing of Anopheles sinensis (Diptera: Culicidae). Korean J. Entomol. 33, 267-271 https://doi.org/10.1111/j.1748-5967.2003.tb00080.x
  14. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876-82 https://doi.org/10.1093/nar/25.24.4876
  15. Yan, R., S. Small, C. Desplan, C. R. Dearolf and Jr., J. E. Darnell (1996) Identification of a stat gene that functions in Drosophila development. Cell 84, 421-430 https://doi.org/10.1016/S0092-8674(00)81287-8
  16. Zhang, J. G., A. Farley, S. E. Nicholson, T. A. Willson, L. M. Zugaro, R. J. Simpson, R. L. Moritz, D. Cary, R. Richardson, G. Hausmann, B. J. Kile, S. B. Kent, W. S. Alexander, D. Metcalf, D. J. Hilton, N. A. Nicola and M. Baca (1999) The conserved SOCS box motif in suppressors of cytokine signaling binds to elongins B and C and may couple bound proteins to proteasomal degradation. Proc. Natl. Acad. Sci. 96, 2071-2076