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Comparison of Physicochemical Properties and Release Characteristics of Extruded Tissue Cultured Mountain Ginseng (압출성형 산삼배양근의 이화학적 성질 및 침출특성의 비교)

  • Han, Jae-Yoon;Chung, Ki-Hwa;Ryu, Gi-Hyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.8
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    • pp.1018-1024
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    • 2008
  • The objective of this study is to compare the physicochemical properties and release characteristics of red ginseng (A) and tissue cultured mountain ginseng (B) extruded tissue cultured mountain ginsengs at barrel temperatures 110 (C) and $120^{\circ}C$ (D) to produce tissue cultured mountain ginseng-like comercial red ginseng by extrusion process. Extrusion process variables, water content and screw speed were fixed at 25% and 200 rpm, respectively. In the results, reducing and total sugar content were found to be relatively higher in A. The acidic polysaccharides content of B was the lowest among the ginseng samples. Acidic polysaccharide was increased 3 times by extrusion process. A and B were three times higher at maximum than C and D in polyphenolic compound. Polyphenolic compound content was relatively low by extrusion of ginsengs. Amino acid contents of B, C and D were $35{\sim}42\;{\mu}g/mL$; in contrast, A contained $25\;{\mu}g/mL$. The crude saponin content of C and D were higher than A and B.

Comparison of Malonyl Ginsenoside Contents in Parts of Korean Ginseng (고려인삼의 부위별 Malonyl Ginsenoside 함량 비교 분석)

  • Park, Young Sik;Oh, Myeong Hwan;Lee, Hwan;Jung, Jong Tae;Jo, Yun Ho;Pyo, Mi Kyung
    • Korean Journal of Pharmacognosy
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    • v.48 no.1
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    • pp.82-87
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    • 2017
  • Malonyl ginsenoside content of the Panax ginseng C.A. Meyer is known to account for 35% to 60% of total ginsenosides content. However, its distribution by ginseng part has not been studied. In this study, four kinds of malonyl ginsenosides were compared in Korean white ginseng part using the purified malonyl ginsenoside standards in our laboratory. White ginseng was prepared by the freeze drying ($-70^{\circ}C$, 48 h) or air drying ($50^{\circ}C$, 48 h) methods form 4-year-old ginseng. Malonyl ginsenoside content of main, lateral, and fine root, and of the main root without skin and its skin was compared. Malonyl ginsenosides (m-Rb1, m-Rb2, m-Rc and m-Rd) content (58%, 19.17 mg/g) in total ginsenosides of air dried white ginseng was decreased about 4% compared to its content of freeze dried white ginseng (62%, 20.40 mg/g). Malonyl ginsenoside content was the highest in fine root, compared to the main or lateral root. Malonyl ginsenosides content in skin of main root was 20.08 mg/g, while its content of the main root without skin was 2.58 mg/g. These results are expected to help establishment of quality specification and processing process in Korean white ginseng.

Effects of C on the Strength and Toughness of FCAW Weld Metal of YS 460 MPa Steels for Ship and Offshore Structures (선박·해양 구조물용 YS 460MPa 강재 FCAW 용접금속의 강도와 인성에 미치는 C의 영향)

  • Jeong, Sang-Hoon;Eom, Jeong-Ho;Choi, Han-Geul;Jeong, Byung-Ho;Hur, Sung-Hwa;Kang, Chang-Yong
    • Journal of Welding and Joining
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    • v.32 no.6
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    • pp.29-34
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    • 2014
  • This paper has an purpose to study the effect of C on the toughness of YS 460 MPa FCAW weld metal. These effects were evaluated by charpy impact and CTOD test about 4 FCAW weld metal containing various C and Si content in relation to microstructure. Increase of C content was helpful to increase AF volume fraction and reduce PF(G) and FS volume fraction by increasing super cooling rate for ferrite transformation. Also, Increase of C content up to 0.045wt% made the strength and impact toughness higher by increasing AF volume fraction. The weld metal containing higher C content indicated higher CTOD value. It is because the volume fraction of PF(G) and FS, can play a role as crack initiation site, was reduced. Effect of C on the strength and elongation of weld metal was higher with an increase of Si contents.

Chemical Composition of Main Cordyceps species in Korea

  • Hong, In-Pyo;Nam, Sung-Hee;Sung, Gyoo-Byung;Lee, Kwang-Gil;Cho, Soo-Muk;Seok, Soon-Ja;Hur, Hyeon;Lee, Min-Woong;Guo, Shun-Xing
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.1
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    • pp.13-17
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    • 2009
  • The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Cordyceps militaris, C. pruinosa and Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori) host were used in this examination. This study was carried out to investigate the soluble sugar, amino acid and fatty acid profiles in the fruiting-bodies. Soluble sugars such as glycerol, glucose, mannitol and sucrose were mainly found in the fruiting bodies of C. militaris, C. pruinosa and P. tenuipes. Total soluble sugar content was 29.23 mg/g in C. militaris, 8.61 mg/g in C. pruinosa and 24.00 mg/g 1in P. tenuipes on dry weight basis. Total free amino acid content was 14.09 mg/g 1in C. militaris, 34.60 mg/g in C. pruinosa and 17.09 mg/g in P. tenuipes. The content of oleic acid in fatty acids was above high more than 30% regardless of species.

Optimal Extraction Conditions to Produce Rosemary Extracts with Higher Phenolic Content and Antioxidant Activity (폴리페놀 함량과 항산화력에 따른 로즈마리의 최적 추출조건 확립)

  • Lee, Chang-Yong;Kim, Ki-Myeong;Son, Hong-Seok
    • Korean Journal of Food Science and Technology
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    • v.45 no.4
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    • pp.501-507
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    • 2013
  • We evaluated the effects of temperature, time, solvent type, and solvent concentration on the phenolic content and antioxidant activity of rosemary extracts. The antioxidant capacity of rosemary extracts was demonstrated by their ability to scavenge stable free radicals, and the phenolic content of the extracts was determined using the Folin Ciocalteu method. The highest values were obtained by water-based extraction at $90^{\circ}C$ for 30 min, a 75% methanol solution at $60^{\circ}C$ for 30 min, and a 50% ethanol solution at $70^{\circ}C$ for 10 min. The aqueous solution of 75% methanol, extraction temperature of $60^{\circ}C$, and extraction time of 30 min were the most efficient parameters for the extraction of polyphenols from dry rosemary.

Effect of Cooked Glutinous Rice Flour and Soused Shrimp on the Changes of Free Amino Acid, Total Vitamin C and Ascorbic Acid Contents during Kimchi Fermentation (찹쌀풀 및 새우젓 첨가가 김치 발효중 총 유리아미노산, 총 Vitamin C 및 환원형 Ascorbic acid의 함량변화에 미치는 영향)

  • Lee, Hyung-Ok;Lee, Hye-June;Woo, Soon-Ja
    • Korean journal of food and cookery science
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    • v.10 no.3
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    • pp.225-231
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    • 1994
  • This study was intended to observe the effect of cooked glutinous rice flour and soused shrimp on the fermentation of Kimchi samples were fermented for 11 days at 10$^{\circ}C$ after they were previously fermented for 6 hours at 20$^{\circ}C$. Changes of pH, total acidity, salt content, reducing sugar content, free amino acid content, total vitamin C content, and ascorbic acid content were measured. The results were as follows; 1. Soused shrimp and glutinous rice flour added in Kimchi acted as lactobacilli growth enhancer during fermentation. 2. Initial cincentrations of redng sugars in Kimch samples with no soused shrimp, 5% and 10% sosed shrimp were 7.5~8.3 mg/g, 3.0~4.7 mg/g and 0.5~0.6 mg/g respectively. Kinchi samples with glutinous rice flour showed higher reducing sugar contents than Kimchi samples without it. 3. Free amino acid contents in Kimchi samples gradually decreased during fermentation at 10$^{\circ}C$. According to their soused shrimp concentration, contents of free amino acid of Kimchi samples were significantly different. And Kimchi samples with glutinous rice flour showed lower free amino acid contents than Kimchi samples without it. 4. Total vitamin C contentrations of Kimchi samples were 24.5~28.0 mg% at the initial stage of fermentation and 15.0~19.2 mg% at the final stage of fermentation. During the fermentation of Kimchi, the higher soused shrimp concentration, the less total vitamin C contents were remained. 5. During the fermentation, ascorbic acid contents of Kimchi samples were not affected by soused shrimp and glutinous rice flour.

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Change of Physicochemical Characteristics of Korean Pear(Pyrus pyrifolia Nakai) Juice with Heat Treatment Conditions (열처리 조건에 따른 한국산 배(Pyrus pyrifolia Nakai)즙의 이화학적 특성 변화)

  • Hwang, In-Guk;Woo, Kwan-Sik;Kim, Tae-Myoung;Kim, Dae-Joong;Yang, Mi-Hye;Jeong, Heon-Sang
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.342-347
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    • 2006
  • Korean pear juice heat-treated at various temperatures (110, 120, 130, 140, and $150^{\circ}C$) and times (1, 2, 3, 4, and 5 hr) was analyzed to determine total polyphenol, total flavonoid, free sugar, and 5-HMF contents, and DPPH free radical-scavenging activity. Total polyphenol, total flavonoid, and 5-HMF contents, and antioxidant activities increased, whereas fructose content decreased with increasing heat treatment temperature and time ($p\;{\pm}\;0.007\;mg/g$ with treatment at $150^{\circ}C$ for 1 hr (unheated pear juice: $0.233\;{\pm}\;0.024\;mg/g$). Highest total flavonoid content was $561.49\;{\pm}\;16.93\;{\mu}g/g$ with treatment at $150^{\circ}C$ for 4 hr (unheated pear juice: $1.50\;{\pm}\;0.20\;{\mu}g/g$). $IC_{50}$ value was highest, $1.93\;{\pm}\;0.01\;mg/g$, at $150^{\circ}C$ for 2 hr, whereas that of unheated pear juice was $64.58\;{\pm}\;0.99\;mg/g$. Glucose content was not affected by treatment conditions, whereas fructose content decreased with treatment at $140^{\circ}C$ for 3 hr. Highest 5-HMF content was $5,594.93\;{\pm}\;6.43\;ppm$ at $150^{\circ}C$ for 5 hr (unheated pear juice: $2.04\;{\pm}\;0.21\;ppm$). Correlation coefficients were observed among total phenolic, total flavonoid, and antioxidant activities, and between fructose and 5-HMF at significance level (p<0.001).

Identification of C3G(cyanidin-3-glucoside) from Mulberry Fruits and Quantification with Different Varieties (오디에서 C3G(cyanidin-3-glucoside)의 분리, 동정 및 계통별 함량분석)

  • 김현복;김선림
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.90-95
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    • 2003
  • This study was carried out to identify of C3G (cyanidin-3-glucoside) from mulberry fruits and quantify with different varieties. C3G of mulberry fruits was extracted with 1% HCl-MeOH and purified with open column (5${\times}$90cm) which filled with Amberlite IRC-50 ion exchange resin. The $\lambda$max ranges of the purified C3G on UV/vis spectrum were 516nm and 280nm. Also, molecular weight of C3G from mulberry fruits by LC-Mass was determined as 449. From above results, we concluded that anthocyanin pigment of mulberry fruits was C3G only. The cyanidin-3-glucoside (C3G) was separated and quantified by High Performance Liquid Chromatography (HPLC) system using a Nova-Pack C$\_$18/ column. Mean content of the 35 tested accessions was 0.89%. Also fruity characteristics as well as C3G content to select the desirable mulberry varieties for the production of fruit were researched and analyzed. We selected three suitable varieties such as 'Susungppong', 'Kangsun', and 'Jeolgokchosaeng(Chungpuk)'.

Chemical Composition of Korean Geoduck and Changes in Their Composition during Frozen Storage (코끼리조개의 성분 조성과 냉동 저장 중의 성분 변화)

  • Choi, Hung-Gil
    • Journal of Fisheries and Marine Sciences Education
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    • v.3 no.2
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    • pp.47-72
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    • 1991
  • To obtain the principal data for useful treatment and processing of Korean geoduck (Panope japonica A. ADAMS) which inhabit mostly at Dong-Hae coastal area in Korea, changes of $NH_2$-N, TMAO, TMA, total creatinine, protein composition and fatty acid composition in raw and blanched geoduck muscle during storage at $-20^{\circ}C$ were investigated. In addition, its chemical composition variation in the whole year was elucidated. The moisture content in geoduck muscle meat was 78.1% to 82% in the whole year. Particularly, in July its moisture content was maximum as 82% and in September minimum as 78.1%. Crude protein was in the range of 12.3-16.4%, crude lipid the average was 1.5%, crude ash on the average was 1.4%. The abundant fatty acids in geoduck muscle oil were $C_{16}$ : 0, $C_{16}$ : 1, $C_{18}$ : 0, $C_{18}$ : 1, $C_{20}$ : 5, and $C_{22}$ : 6 acids. During storage at $-20^{\circ}C$, content of unsaturated fatty acid such as eicosapentaenoic acid (EPA, $C_{20}$ : 5) and docosahexaenoic acid (DHA, $C_{22}$ : 6)in raw geoduck muscle decreased somewhat and the raw geoduck was slightly oxidized. Trimethylamine (TMA), volatile basic nitrogen (VBN)and $NH_2$-N of raw muscle increased compared to blanched muscle. Trimethylamine oxide (TMAO) was slightly decreased during the storage period. The muscle protein was approximately composed of 37% sarcoplasmic, 29% myofibrillar, 22% alkali soluble, and 12% stroma protein. Among several proteins, myofibrillar protein content decreased mostly, while the alkali-soluble and stroma protein content increased slightly during storage at $-20^{\circ}C$.

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Analysis of γ-Aminobutyric Acid Content in Fermented Plant Products by HPLC/UV

  • Lee, Dong Gu;Cho, Sunghun;Lee, Jamin;Cho, Seon Haeng;Lee, Sanghyun
    • Journal of Applied Biological Chemistry
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    • v.58 no.4
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    • pp.303-309
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    • 2015
  • ${\gamma}$-Aminobutyric acid (GABA) content in fermented plant products and their main plant materials (aerial part of Acanthopanax sessiliflorus, fruit of Crataegus pinnatifida, and whole plant of Morus alba) was determined by high-performance liquid chromatography. GABA was quantified using a reverse-phase column with a gradient elution program (water:acetonitrile =90:10 to 0:100 for 40 min). UV detection was conducted at 280 nm. GABA content was measured in fermented plant products (15.07 mg/g), aerial part of A. sessiliflorus (4.49 mg/g), fruit of C. pinnatifida (10.59 mg/g), and whole plant of M. alba (2.31 mg/g). The presence of GABA in fermented plant products, including A. sessiliflorus, C. pinnatifida, and M. alba is important in industrial application for health supplements.