• Journal of the Korean Wood Science and Technology
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• 제41권6호
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• pp.559-565
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• 2013

목재사용량의 증가에 따라 목재의 화재안전성에 대한 요구가 높아지고 있다. 실내 사용 목재의 난연처리에 무기인계를 약제로 사용함으로써 환경에 대한 부담을 줄이면서 난연성능을 향상 시킬 수 있다. 수용성 약제를 가압 주입하여 목재 내 깊숙이 침투시키면 목재의 고유기능을 유지하면서 난연성능을 부여 할 수 있다. 난연제의 용탈을 억제하여 장기간 성능을 유지할 수 있는 장점도 있다. 저자들은 액상규산나트륨을 주약제로 하고, 붕산 및 인계 약제를 보조약제로 하여 목재 내로 약액을 주입하는 공정을 개발하였다. 이들 약제는 난연성능 외에 방부 및 방충성능을 지니는 약제가 포함되어 있어, 난연성능과 함께 목재 사용 시 노출될 수 있는 부후환경에서의 내후성능을 방미효력과 방부효력으로 평가하였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.21-22
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• 2003

Fungicide treatment is the most important method for the control of plant diseases caused by phytopathogenic fungi. But fungicide resistant strains have appeared in many phytopathogenic fungi. Until now, molecular mechanisms of fungicide resistance such as mutation of target protein, overproduction of target enzyme and detoxification of fungicide have been designated. Recently, it was demonstrated that active efflux of fungicides mediated by ATP-binding cassette (ABC) transporters also contributes to fungicide resistance in several filamentous fungi, such as Aspergillus nidulans, Penicillium digitatum and Botrytis cinerea.(중략)

• 원예과학기술지
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• 제32권5호
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• pp.673-683
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• 2014

2010년 대전에서 채집한 흰가루병이 발생한 오이 잎의 단일 병반에서 흰가루병균을 분리하였고, 이 흰가루병균은 광학현미경 하에서의 형태학적 특성과 멜론 판별품종에서의 저항성 반응에 따라 Podosphaera xanthii race 1으로 동정되었다. 박과 작물 흰가루병에 대한 저항성 검정에 효율적인 생육시기를 결정하기 위하여, P. xanthii race 1에 대한 오이품종들의 생육시기에 따른 저항성 차이를 조사하였다. 시판중인 오이 34개 품종의 이 흰가루병균에 대한 저항성을 온실에서 3종류의 생육시기에서 실험한 결과, 떡잎에서의 저항성 반응은 본엽에서와 일치하지 않았으며, 본엽 1엽에서의 흰가루병 발생 정도는 식물 전체의 흰가루병 발생량과 높은 상관관계를 보였다. 따라서 본엽 1엽에 흰가루병이 충분히 발생하는 시기에 1엽만을 대상으로 저항성을 검정하는 것이 효율적이라 생각되었다. 또한, 박과 작물의 재배 시기에 따른 저항성 차이를 조사하기 위하여 오이 12개 품종을 4개의 재배 시기에 그리고 멜론 26개 품종을 3개의 재배시기에 흰가루병 발생을 조사하였다. 오이 품종들 중 6개 품종은 재배 시기에 따라 흰가루병 저항성에 차이를 보였으며, 온도가 올라가면 저항성이 증가함을 알 수 있었다. 이와 달리 멜론 품종들은 실험한 품종 중 5개가 고도의 저항성을 보였으며, 이들의 흰가루병 저항성은 재배 시기에 따라 거의 차이를 나타내지 않았다. 한편, 대전에서 분리한 P. xanthii race 1 균주는 수박 7개 품종에 대하여 병원성을 나타내지 않았다. 하지만 모든 P. xanthii race 1 균주가 수박에 비병원성인지는 race 1 균주를 더 많이 확보하여 확인하는 것이 필요하다.

• The Plant Pathology Journal
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• 제16권2호
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• pp.105-109
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• 2000

The Pi-b is the rice gene conferring race specific resistance to the blast fungus Magnaporthe grisea race having a corresponding avirulence gene, AVR-Pi-b. All resistant cultivars have two copies of the Pi-b gene, but susceptible cultivars have a single copy of the gene. About 1 Kbp insertion sequence was detected in the open reading frame of the Pi-b gene from the susceptible cv. Nipponbare. The nature of insertion sequence was identified as a solo long terminal repeat (LTR) of new rice Tyl-copia-like retrotransposon. LTR was widely distributed in the rice genome. Various types of different patterns of restriction fragment length polymorphism of LTR were detected in indica cultivars, whereas a single type was detected from japonica cultivars. The insertion of LTR sequence in the Pi-b gene in the susceptible cultivar suggested that retrotransposon-mediated insertional mutation might played an important role in the resistance breakdown as well as evolution of resistance genes in rice.

• Mycobiology
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• 제45권4호
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• pp.426-429
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• 2017

A yeast-like organism was isolated from a urine sample of a 6-year-old neutered male miniature poodle dog with urinary tract infection, diabetes ketoacidosis, and acute pancreatitis. We identified the yeast-like organism to be Candida glabrata and found that this fungus was highly resistant to azole antifungal drugs. To understand the mechanism of azole resistance in this isolate, the sequences and expression levels of the genes involved in drug resistance were analyzed. The results of our analysis showed that increased drug efflux, mediated by overexpression of ATP transporter genes CDR1 and PDH1, is the main cause of azole resistance of the C. glabrata isolated here.

• The Plant Pathology Journal
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• 제26권4호
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• pp.421-423
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• 2010

New sources of barley (Hordeum vulgare L.) resistant to spot blotch, caused by Cochliobolus sativus, are needed to provide effective resistance because of the rapid change pathotype patterns of C. sativus in fields. The purposes of our study were to develop a method to screen barley for resistance to spot blotch disease and then use this methodology to screen barley genotypes for resistance to the major virulent pathotype Pt4 in barley populations in Syria. A transparent tape method, in which a conidial suspension of C. sativus was dropped onto transparent tape and placed, treated-side down, on the second leaf surface of barley plants. Disease symptoms of fungus were easily detected on the leaves covered by the transparent tape after 48h of inoculation. The transparent tape method was repeatable and the disease scores obtained were correlated (r = 0.91, P = 0.001) with those obtained by the seedling assay. This method may be beneficial in various plant pathology breeding programs.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.167-167
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• 2002

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.243-243
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• 2005

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2000년도 추계 학술발표회
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• pp.28.1-28
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• 2000

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.134-134
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• 2017

Recently, host-induced gene silencing (HIGS) system has been successfully applied into development of resistant crops against insects, fungal and viral pathogens. To test HIGS-mediated resistance in rice against rice blast fungus, Magnaporthe oryzae, we first tested possibility of movement of small non-coding RNA from rice cells to rice blast fungus. The rice blast fungus expressing GFP transgene were inoculated to transgenic rice plants ectopically expressing dsRNAi construct targeting fungal GFP gene. Expression of dsRNAi construct for GFP gene in transgenic plants significantly suppressed GFP expression in infected fungal cells indicating that small RNAs generated in plant cells can move into infected fungal cells and efficiently suppress the expression of fungal GFP gene. Consistent with these results, expression of dsRNAi constructs against 3 fungal pathogenic genes of M. oryzae in transgenic rice specifically and efficiently suppressed not only the expression of fungal pathogenic genes, but also fungal infection. The conidia of M. oryzae applied on leaf sheath of transgenic rice expressing dsRNAs against 3 fungal pathogenic genes showed abnormal development of primary hyphae and malfunction of appressorium, which is consistent with the phenotypes of corresponding fungal knock-out mutants. Taken these results together, here, we suggest a novel strategy for development of antifungal crops by means of HIGS system.