• 대한미생물학회지
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• 제7권1호
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• pp.55-58
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• 1972

The determination of fungi in various kind of rice-cakes have been carried out in order to information of the toxigenic fungi in Korean rice-cakes. The results as follows: 1. Predominant genera of fungi in most rice-cakes were Pencillium sp.(32.2%) and Aspergillus sp.(30.1%). 2. Of the 63 various rice-cakes, 143 strains of fungi were isolated and 135 strains were possible to identify into 9 genera. 3. Artemisia asiatics and some kind of colouring matters have been an antifungal activity.

• Mycobiology
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• 제33권4호
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• pp.167-172
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• 2005

Through ten field surveys in Kanghwa Island from August of 1997 to March of 2002, total 107 specimens of wood-rotting fungi belonging to the Aphyllophorales were collected and identified to the species. They taxonomically amounted to 10 families, 31 genera, and 48 species. Among them, one family, Steccherinaceae, and four genera, Australohydnum, Castanoporus, Dacryobolus and Haplotrichum were confirmed as new to Korea. Five unrecorded species, Australohydnum dregeanum, Castanoporus castaneus, Dacryobolus karstenii, Haplotrichum conspersum and Hyphoderma odontiiforme were registered here with descriptions.

• Mycobiology
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• 제37권2호
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• pp.147-151
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• 2009

The wood-rotting fungi of three Korean islands in the Yellow Sea, Soyeonpyung-do (SY), Oeyeon-do (OY), and Gageo-do (GG), were investigated from October 2004 to November 2005. During four sampling trips on each island, a total of 46, 44, and 44 specimens were collected from SY, OY, and GG, respectively. The identified wood-rotting fungi from SY included 29 species of 22 genera and nine families; OY had 31 species of 26 genera and 10 families; and GG had 34 species of 27 genera and 11 families. The majority of the fungi were in the Polyporaceae, which was represented by 23 genera and 30 species. Auricularia polytricha, Daedaleopsis tricolor, Daldinia concentrica, Hymenochaete cinnamomea, Hymenochaete yasudai, Hyphoderma setigerum, Lopharia mirabilis, Schizopora paradoxa, and Trametes versicolor were collected from all three islands.

• 한국균학회지
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• 제42권4호
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• pp.344-348
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• 2014

A total of 118 fungal specimens were collected from Mt. Manggyeong, Chungnam province, Korea between July and October 2014. All specimens were identified to the species level using a combination of morphological characteristics and DNA sequencing. Out of 118 specimens, eight species, Amanita manginiana, A. pilosella, Calvatia holothurioides, Cantharellus tabernensis, Dicephalospora rufocornea, Gymnopus menehune, Marasmius brunneospermus, and Russula cerolens, have not been previously reported in Korea.

• Mycobiology
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• 제49권5호
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• pp.454-460
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• 2021

Daecheongdo and Socheongdo Islands are located in the West Sea of Korea, 210 km away from land, and are military border areas very close to North Korea, making them difficult to access. Although the ecosystem of the islands is relatively well preserved due to the lack of accessibility, research on fungi of the regions is insufficient. Therefore, we aimed to investigate indigenous fungi in these geographically and geopolitically constrained regions. A survey of the indigenous fungal diversity of the islands was conducted in 2018. All specimens were identified at the species level based on morphological and molecular analyses. Among them, six macrofungi-namely, Agaricus menieri, Crepidotus praecipuus, Dichomitus squalens, Hortiboletus amygdalinus, Melanoleuca friesii, and Trametes lactinea-were not previously reported in Korea. Considering that the proportion of unrecorded species is high in the survey area and period as well as the number of samples collected, similar research on adjacent islands may be necessary.

• 한국균학회지
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• 제19권2호
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• pp.109-119
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• 1991

송이 (Tricholoma matsutake) 생산의 대표적인 지역으로 알려진 강원도 양양 지역과 명주 지역을 선택하여 송이 발생지와 미발생지의 미세균류를 분리 동정하였으며 수직분포를 조사하였다. 진균의 선별방법으로 희석법, $42^{\circ}C$에서 48시간 배양 후 $37^{\circ}C$로 배양하는 방법, $70^{\circ}C$에서 15분간 열처리 방법, 그리고 ethanol처리 방법으로 토양의 수직적 분포를 파악하였다. $42^{\circ}C$배양법에서는 두 장소에서 분리된 토양균은 7속 18종이 분리되었으며, 송이 발생지에서는 Aspergillus fumigatus, A. ochraceus, A. terreus, Acremonium sp., Penicillium frequentans, Talarom-yces stipitatus가 분리되었으며, 송이 미발생지에서는 Aspergillus fumigatus, Penicillium lilacinum, P. oxalicum, Westerdykella multispora가 우점종으로 나타났다. $70^{\circ}C$열처리 방법으로 분리한 경우 7속20종이 분리되었으며, 송이발생지에서는 Aspergillus fumigatus, Mucor sp. 가 우점적으로 나타났으며, 송이 미발생지에서는 A. iumigatus, Atternaria alter-nate, Mucor sp., Neurospora sitophita가 우점종으로 나타났다. Ethanol처리 방법으로 분리한 경우는 1속 1종이 분리되었으며, 송이 발생지와 미발생지에 관계없이 우점종은 Mortierella sp.로 분류되었다. 이들 분리된 토양균은 전체균의 수와 우점종균수 그리고 나타나는 종의 빈도수는 그 수직적 분포에 있어서 상층에서 하층으로 내려갈수록 감소하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권10호
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• pp.1528-1539
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• 2019

Objective: To evaluate the effects on microbial diversity and biochemical parameters of gradually increasing temperatures, from $5^{\circ}C$ to $25^{\circ}C$ on corn silage which was previously fermented at ambient or low temperature. Methods: Whole-plant corn silage was fermented in vacuum bag mini-silos at either $10^{\circ}C$ or $20^{\circ}C$ for two months and stored at $5^{\circ}C$ for two months. The mini-silos were then subjected to additional incubation from $5^{\circ}C$ to $25^{\circ}C$ in $5^{\circ}C$ increments. Bacterial and fungal diversity was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiling and biochemical analysis from mini-silos collected at each temperature. Results: A temperature of $10^{\circ}C$ during fermentation restricted silage fermentation compared to fermentation temperature of $20^{\circ}C$. As storage temperature increased from $5^{\circ}C$ to $25^{\circ}C$, little changes occurred in silages fermented at $20^{\circ}C$, in terms of most biochemical parameters as well as bacterial and fungal populations. However, a high number of enterobacteria and yeasts (4 to $5\;log_{10}$ colony forming unit/g fresh materials) were detected at $15^{\circ}C$ and above. PCR-DGGE profile showed that Candida humilis predominated the fungi flora. For silage fermented at $10^{\circ}C$, no significant changes were observed in most silage characteristics when temperature was increased from $5^{\circ}C$ to $20^{\circ}C$. However, above $20^{\circ}C$, silage fermentation resumed as observed from the significantly increased number of lactic acid bacteria colonies, acetic acid content, and the rapid decline in pH and water-soluble carbohydrates concentration. DGGE results showed that Lactobacillus buchneri started to dominate the bacterial flora as temperature increased from $20^{\circ}C$ to $25^{\circ}C$. Conclusion: Temperature during fermentation as well as temperature during storage modulates microorganism population development and fermentation patterns. Silage fermented at $20^{\circ}C$ indicated that these silages should have lower aerobic stability at opening because of better survival of yeasts and enterobacteria.

• 한국균학회지
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• 제16권1호
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• pp.26-32
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• 1988

성공적인 결핵치료에도 불구하고 계속적인 만성기침 또는 객담이 있거나 간헐적인 혈담 또 각혈이 있는 환자와 그외에도 진균감염이 의심되는 환자 등 총 1,192명을 대상으로 하여 모든 환자로부터 혈청을 채취하고 대부분의 환자로부터 객담이나 기타 병리가검물을 채취하여 흉부 x선소견과 함께 진균감염여부를 관찰하였다. 면역확산법검사에 의해 한가지 이상의 진균항원에 대해 양성반응을 나타내는 환자는 전체의 34.0%인 405명이고 그중에서 Aspergilli에 감염된 환자는 303명이었고, 대부분의 환자(287명)가 Aspergillus fumigatus에 감염되어 있고 그 다음은 Aspergillus flavus(1.7%) Aspergillus nidulans(0.3%), Aspergillus niger(0. 3%), Aspergillus nidulans var. latus(0.1%)순이며 Candida albicans(8.1%)에도 침강항체반응이 나타났으며 Pseudallerscheria boydii 드물게 분리되는데 9명(8.1%)에도 침강항체반응을 나타내었다. 침강항체반응 양성을 나타내는 186명의 흉부 x선사진에서 진균종 음영이 나타나는 환자가 47명이었고 이들 가운데서 45명이 A. fumigatus 의해 형성되었다. 진균감염 또는 미감염환자 724명의 객담에서 발견되는 진균가운데 46.4%가 Aspergilli였다. 413명의 환자객담에서 분리된 137주의 효모양균주를 분리 동정할 결과 그중 Candida albicans(64.2%)가 가장 많았다.

• 한국균학회지
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• 제40권4호
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• pp.288-291
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• 2012

한국에서 발병하는 참나무시들음병은 암브로시아 곤충인 Platypus koryoensis.에 의해 전반되는 Raffaelea quercus-mongolicae 균류에 의해 발생한다. 이 병의 전파를 방지하기 위해 참나무시들음병 감염목을 벌채 후 비닐막을 씌워 훈증하고 벌채장소에 3년 이상 방치시키고 있다. 본 연구는 이런 훈증처리된 감염목에 존재하는 균류에 대한 정보를 얻고자 수행하였다. 천안 태조산 지역 두 곳에서 훈증처리된 감염목을 채집하고 진균을 분리하여 99개 균주를 얻었다. 분리 균주를 형태적 특성과 translation elongation factor 1-alpha 유전자와 ITS rDNA region 염기서열에 의거한 분자적 방법으로 동정한 결과 Hypocrea, Trichoderma, Penicillium 속에 속하는 종들이 확인되었다. 이중 Trichoderma 속에 속하는 종이 주요 균류이었다. 조사결과 병원균인 R. quercus-mongolicae와 매개충인 P. koryoensis는 검출되지 않았다. 본 연구결과는 훈증 처리한 참나무시들음병 감염목이 3년 이상 되면 더 이상의 피해를 유발할 위험요소가 없다는 과학적 증거를 제시한다.

• Mycobiology
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• 제45권3호
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.