• Title/Summary/Keyword: Fundamental act of agriculture

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A Study on the Legal Status of Insect Industry (곤충산업의 법적 지위에 대한 연구)

  • Kim, Yun-Shik;Park, Chung Gyoo;Kim, Taeyoung;Choi, Jong Woo
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.401-408
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    • 2018
  • The insect industry in Korea has been supported by the Act of Fostering and Supporting Insect Industry since 2010. Based on the Act, insect breeders are entitled as farmers. However, the insect industry is not included in the Fundamental Act of Agriculture, Rural Community and Food Industry which defines all concepts, such as agriculture, farmers, and agricultural products. Legally and strictly speaking, there is no method to defense the insect industry when an argument is raised that insect industry is not included into agriculture, so persons who breed insects are not eligible for all agricultural supports and subsidies of the government. Thus, it is necessary to clear the legal status of insect industry by revising the related laws and regulations.

Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

  • El-Rahman, Atef Abd El-Mohsen Abd;El-Shafei, Sally Mohamed Abd El-Aziz;Ivanova, Elena Vladimirovna;Fattakhova, Alfia Nurlimanovna;Pankova, Anna Victorovna;El-Shafei, Mohamed Abd El-Aziz;El-Morsi, El-Morsi Abu El-Fotouh;Alimova, Farida Kashifovna
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.17
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    • pp.7229-7234
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    • 2014
  • Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.

The Application of Dual Function Organoclay on Remediation of Toxic Metals and Organic Compounds in Soil-Water System (양친매성 유기점토를 이용한 중금속과 유기 오염물질 동시제거 기술)

  • Ok, Yong-Sik;Lim, Soo-Kil;Kim, Jeong-Gyu
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.177-184
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    • 2003
  • Although clay can sorb significant amounts of inorganic contaminants from soils and wastewater, the hydration of exchangeable cations in clay minerals makes it hydrophilic at the clay mineral surfaces and interlayers. Thus, natural clays are often ineffective in complexing and stabilizing toxic organic contaminants in soils and groundwater environment. But, substituting these hydrated cations with cationic surfactant such as QAC(Quaternary ammonium Compound) can change the natural clay from hydrophilic to hydropobic. Furthermore functionalized organoclay can act as a powerful dual function sorbent for both toxic metals and organic compounds. It also can be used as landfill clay liners, slurry walls, nano-composite materials, petroleum tank farms, waste treatment, and filter systems. To use this modified clay minerals effectively, it is required to understand the fundamental chemistry of organoclay, synthetic procedures, its engineering application, bioavailability of sorbed ion-clay complex, and potential risk of organoclay. In this review, we investigate the use, application and historical background of the organoclay in remediation technology. The state-of-the-art of organoclay research is also discussed. Finally, we suggest some future implications of organoclay in environmental research.