• Title/Summary/Keyword: Functional capacity

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The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

  • Liu, Di;Zhang, Ting;Chen, Zhifei;Wang, Ying;Ma, Shuang;Liu, Jiyun;Liu, Jingbo
    • Journal of Ginseng Research
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    • v.41 no.2
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    • pp.169-179
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    • 2017
  • Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

Changes of Functional Properties of Acylated Fish Protein (Acyl화에 의한 어류 단백질의 이화학적 성질의 변화)

  • Bang, Chan-Sik;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.33 no.1
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    • pp.52-61
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    • 1990
  • Fish protein was acylated with acetic anhydride(AA), succinic anhydride(SA) and maleic anhydride(MA) in order to improve the functional properties of the protein. The surface hydrophobicity and functional properties of protein were measured to study the relationship between them. It was found that the extented acylation of nucleophilic groups such as amino and sulfhydryl groups of the amino acid residues of fish protein was higher than other groups when acylated with AA, and the degree of acylation was 89.5 % for amino groups and 72.2 % for sulfhydryl groups. The surface hydrophobicity of fish protein was decreased by succinylation and maleylation, whereas acetylation caused tittle change. The acylated fish protein concentrate(FPC) showed higher surface hydrophobicity than the acylated fish myofibrilla protein(FMP). Acylation with AA, SA and MA of fish protein resulted in a significant increase in protein solubility, emulsifier properties, foaming properties, water adsorption capacity and oil adsorption capacity. These properties of acylated FMP were more improved than those of acylated FPC. Decrease in protein hydrophobicity was highly correlated with increase in protein solubility, and emulsifier properties and foaming properties were largely dependent on the solubility as well as surface hydrophobicity. The water adsorption capacity of the protein was significantly affected by solubility. Surface hydrophobicity had greater influence on oil adsorption capacity, whereas it had tittle effect on water adsorption capacity.

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A Study on the Evaluation for the Improvement of the Functional Movement of Women′s Wear -with Concentration on the Measurement of Functional Movement according to the Change of Cap Height- (여성복의 기능성 향상을 위한 착의 평가에 관한 연구 -소매산 높이 변화에 따른 기능성 측정을 중심으로-)

  • 박선경
    • The Research Journal of the Costume Culture
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    • v.12 no.2
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    • pp.249-261
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    • 2004
  • This research proves the ROM method (Range Of Motion), which has already been used in other countries, that measures the capacity of exercise and the adaptation of movement of functional garments for special purpose due to change of the height of basic sleeve sloper. Basically, the ultimate goal of the research is to improve the functional pattern design. Firstly, this research proves the proper application of ROM that is used for the evaluation of fitting test. Therefore, according to this research, the company is able to produce the sample that is appropriate to the various purpose for women garments and betters the evaluation of function of garments. Moreover, it suggests that the evaluation of fitting test satisfies the consumers who desire to try better garments and contributes to the competitions among the companies that bring more improvements to the fashion design.

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High Hydrogen Capacity and Reversibility of K-Decorated Silicon Materials

  • Park, Min-Hee;Ryu, Seol;Han, Young-Kyu;Lee, Yoon-Sup
    • Bulletin of the Korean Chemical Society
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    • v.33 no.5
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    • pp.1719-1721
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    • 2012
  • We have investigated the $H_2$ adsorption structures and binding energies of the metal (M)-doped (M = Li, Na, K, Mg, and Al) silicon complexes, $M-Si_{19}H_{11}$ and $M-Si_{24}H_{12}$, using density functional calculations. Alkali metals are preferred as doping elements because the Mg-Si and Al-$H_2$ interactions are weak. The maximum numbers of $H_2$ molecules that can be adsorbed are four and five for M=Li and K, respectively. We propose that the K-decorated silicon material might be an effective hydrogen storage material with high hydrogen capacity and high reversibility.

A Density Functional Theory Study on a Series of Functionalized Metal-Organic Frameworks (작용기를 치환한 Metal-Organic Frameworks 에 대한 DFT 연구)

  • Kim Dae Jin;Lee Tae Beom;Choi Seung Hun;Lee Eun Seong;Oh Yu Jin;Hye Ji Hye;Kim Ja Heon
    • 한국전기화학회:학술대회논문집
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    • 2005.07a
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    • pp.329-334
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    • 2005
  • In order to find out rational design and synthetic strategies toward efficient hydrogen storage materials, we performed quantum mechanical calculations on a series of the Metal-Organic Frameworks (MOFs) containing functionalized organic linkers. Based on the shape of frontier orbitals and the electrostatic potential map of various MOFs from density functional theory calculations, it was found that the delocalization of electron and asymmetric polarization of the organic linker play an important role in the hydrogen storage capacity of Metal-Organic Frameworks. The prediction of the modeling study could be supported by the hydrogen adsorption experiments using MOF-5 and amine substituted MOF-5, which showed more enhanced hydrogen storage capacity of amine substituted MOF-5 compared with that of MOF-5.

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Multi-Functional Dual-Layer Polymer Electrolytes for Lithium Metal Polymer Batteries

  • Lee, Young-Gi;Ryu, Kwang-Sun;Chang, Soon-Ho
    • ETRI Journal
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    • v.26 no.4
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    • pp.285-291
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    • 2004
  • We prepared a novel multi-functional dual-layer polymer electrolyte by impregnating the interconnected pores with an ethylene carbonate (EC)/dimethyl carbonate (DMC)/lithium hexafluorophosphate $(LiPF_6)$ solution. The first layer, based on a microporous polyethylene, is incompatible with a liquid electrolyte, and the second layer, based on poly (vinylidenefluoride-co-hexafluoropropylene), is submicroporous and compatible with an electrolyte solution. The maximum ionic conductivity is $7{\times}10^{-3}S/cm$ at ambient temperature. A unit cell using the optimum polymer electrolyte showed a reversible capacity of 198 mAh/g at the 500th cycle, which was about 87% of the initial value.

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functional Properties of Sesame Protein Concentrate as Degree of Hydrolysis by Enzyme Treatments (효소처리한 참깨박 농축단백질의 가수분해정도에 따른 기능성)

  • 윤시혜;박정륭;전정례
    • Journal of the East Asian Society of Dietary Life
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    • v.4 no.3
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    • pp.87-96
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    • 1994
  • This study was carried out to investigate the effect of hydrolysis by proteolytic enzymes on the functional properties of sesame protein concentrate. Sesame protein concentrate was hydrolyzed with papain, pepsin and trypsin to obtain 10% and 20% degree of hydrolysis. The nirogen solubility in water was increased with increasing the degree of hydrolysis. Bulk density was increased by enzymatic hydrolysis but water absorption capacity was increased only in the case of pepsin-hydrolyzed SPC. Higher fat absorption capacity was found in SPC with 10% DH than SPC with 20% DH. Emulsifying activity was also increased by enzymatic hydrolysis except SPC with 10% DH by papain.

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A Comparison of Thoracic Gas Volume measured by Body Plethysmographic Method and Functional Residual Capacity measured by Closed Circuit Method (Body Plethysmograph 방법과 폐쇄식회로법으로 측정한 기능적잔기량의 비교)

  • Chung, Chee-Kyung
    • Journal of Preventive Medicine and Public Health
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    • v.3 no.1
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    • pp.17-22
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    • 1970
  • By using Siregnost FD 91 body plethysmograph, we measured thoracic gas volume (TGVe) at end of expiration in 19 healthy subjects aged 20-43 years in order to compare with functional residual capacity (FRC) measured by closed circuit method. The results obtained were as follows 1. Mean values of TGVe and FRC were $3.395{\pm}0.5851{\ell},\;and\;3.393{\pm}0.618{\ell}$, respectively. 2. A advantage of the body phethysmographic method for measuring thoracic gas volume was that it were rapid, safe, and easy to perform, requires no gas sample for chemical analysis, and measured TGVe several times.

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Adsorption of Ammonia on the Sulfuric Acid Treated ACF

  • Kim, K.H.;Shin, C.S.
    • Carbon letters
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    • v.2 no.2
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    • pp.109-112
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    • 2001
  • For the adsorption of ammonia, activated carbon fibers (ACFs) were subjected to sulfuric acid treatment in order to modify the surface functional groups. The surface acid and base value of ACFs were measured using titration and FT-IR spectrometry. SEM was used to investigate the surface morphology. Acid treatments by $H_3PO_4$, $H_2SO_4$, and $HNO_3$ were performed to increase the adsorption capacity of $NH_3$. As a result, Cellulose-based ACF has high adsorption capacity for ammonia. The ammonia removal efficiency of ACF was the maximum which was treated by 15 wt% sulfuric acid at $100^{\circ}C$ for 60 min. The average pore diameter little increased from $19{\AA}$ to $20.8{\AA}$ and the specific surface area of ACF considerably decreased and acid values increased by 15 wt% sulfuric acid treatment. Ammonia reacted with sulfonyl radicals. After adsorption of ammonia, white material was grown on the surface of ACF through the adsorption of ammonia and it was determined to ammonium sulfate.

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FUNCTIONAL PROPERTIES CHANGE OF PIGSKIN COLLAGEN BY CHEMICAL MODIFICATION

  • Lee, M.;Kwon, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.4 no.4
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    • pp.407-410
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    • 1991
  • The relationship between the possible structural change due to chemical modifications and functionality changes was studied in pigskin collagen. Amino groups in collagen were modified by succinylation and reductive alkylation. Carboxyl groups were modified using carbodiimide. Thermal denaturation temperature of collagen increased remarkably by carboxyl groups modification whereas decreased by succinylation and reductive alkylation. Emulsifying capacity was improved by reductive alkylation and carboxyl groups modification while emulsion stability was improved by succinylation. Chemical modifications increased solubility whereas decreased the foaming capacity of collagen. Viscosity of collagen at various pH varied with methods of modification.