• Fisheries and Aquatic Sciences
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• 제19권8호
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• pp.33.1-33.7
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• 2016

Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5'-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of $0.07{\pm}0.01^{\circ}C$ at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.

• 구강회복응용과학지
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• 제24권4호
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• pp.317-324
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• 2008

이 논문의 목적은 전부주조관에서 교합점의 위치와 교합력의 방향이 합착용 시멘트층 내의 응력에 미치는 영향에 대해서 알아보는 것이다. 하악 제 1대구치상에서 서로 다른 9 개의 교합점과 3가지 교합력 방향을 가진 27가지 조합의 유한요소 모델을 상정하였다. 금관의 소재는 제 3형 금합금이고, 변연의 형태는 chamfer이다. 합착용 시멘트로는 전 층에서 균일하게 $70{\mu}m$의 두께를 가지는 글라스 아이오노머 시멘트가 사용되었다. 금관에는 100N의 하중을 적용하였다. 협측과 설측 변연의 근접도에 따라서 시멘트층 내 응력은 다른 양상을 나타내었다. 협측은 변연으로부터 약 0.5 mm, 설측은 변연으로부터 약 1 mm 내측에서 최대 응력 값을 가졌다. 하중 방향이 치축에 대해 경사가 클수록, 하중점이 교두첨에 근접해 있을수록 더 큰 응력이 발생하였다.

• 구강회복응용과학지
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• 제23권4호
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• pp.293-302
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• 2007

I. Objectives The purpose of this study was to compare the shaping ability between the single length technique performed with Mtwo instruments (VDW, Munich, Germany) and the crown-down technique using K3 (SybronEndo, West Collins, CA, USA) and RaCe (FKG, La Chaux-de-Fonds, Switzerland) instruments. II. Materials & Methods Forty five curved canals in resin blocks were equally divided in to three groups. Group 1 (Mtwo) was instrumented used the full length of canal according to the manufacturer's instructions. The simulated canals was prepared to an instrument size of 35, 0.04 taper canal terminus. In group 2 (Race) and group 3 (K3) was instrumented in a crown-down manner and prepared to an instrument size of 30, 0.06 taper canal terminus. Pre- and post-instrumentation images were scanned and assessment of canal shape was completed with a computer image analysis program. Material removal was measured at 7 measuring points, beginning 1mm from the end point of preparation. Differenced of centering ratio were statistically analyzed using One-way ANOVA followed by Duncan's test. II. Results & Conclusion There was no significant difference on 1, 2, 3 and 7mm measuring point. At 4 and 5 measuring point, significant difference showed between the Mtow instruments and other two instruments. (p<0.05)

• 천문학회보
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• 제44권1호
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• pp.78.2-78.2
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• 2019

We develop an Alcock-Paczynski (AP) test method that uses the evolution of redshift-space two-point correlation function (2pCF) of galaxies. The method improves the AP test proposed by Li et al. (2015) in that it uses the full two-dimensional shape of the correlation function. Similarly to the original method, the new one uses the 2pCF in redshift space with its amplitude normalized. Cosmological constraints can be obtained by examining the redshift dependence of the normalized 2pCF. This is because the 2pCF should not change apart from the expected small non-linear evolution if galaxy clustering is not distorted by incorrect choice of cosmology used to convert redshift to comoving distance. Our new method decomposes the redshift difference of the 2-dimensional correlation function into the Legendre polynomials whose amplitudes are modelled by radial fitting functions. The shape of the normalized 2pCF suffers from small intrinsic time evolution due to non-linear gravitational evolution and change of type of galaxies between different redshifts. It can be accurately measured by using state of the art cosmological simulations. We use a set of our Multiverse simulations to find that the systematic effects on the shape of the normalized 2pCF are quite insensitive to change of cosmology over \Omega_m=0.21 - 0.31 and w=-0.5 - -1.5. Thanks to this finding, we can now apply our method for the AP test using the non-linear systematics measured from a single simulation of the fiducial cosmological model.

• Journal of Power Electronics
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• 제4권3호
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• pp.138-151
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• 2004

In this paper, a constant frequency phase shifting PWM-controlled voltage source full bridge-type series load resonant high-frequency inverter using the $4^{th}$ generation IGBT power modules is presented for innovative consumer electromagnetic induction heating applications, such as a hot water producer, steamer and super heated steamer. The bridge arm side link passive capacitive snubbers in parallel with each power semiconductor device and AC load side linked active edge inductive snubber-assisted series load resonant tank soft switching inverter with a constant frequency phase shifted PWM control scheme is evaluated and discussed on the basis of the simulation and experimental results. It is proved from a practical point of view that the series load resonant and edge resonant hybrid high-frequency inverter topology, what is called, DE class type, including the variable-power variable-frequency regulation function can expand zero voltage soft switching commutation area even under low output power setting ranges, which is more suitable and acceptable for newly developed induction heated dual pack fluid heaters. Furthermore, even the lower output power regulation mode of this high-frequency load resonant tank inverter circuit is verified so that this inverter can achieve ZVS with the aid of the single auxiliary inductor snubber.

• Journal of Photoscience
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• 제12권1호
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• pp.1-9
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• 2005

The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

• JSTS:Journal of Semiconductor Technology and Science
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• 제1권4호
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• pp.209-215
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• 2001

In this paper a method for analysis and modelling of coplanar transmission interconnect lines that are placed on top of silicon-silicon oxide substrates is presented. The potential function is expressed by series expansions in terms of solutions of the Laplace equation for each homogeneous region of layered structure. The expansion coefficients of different series are related to each other and to potentials applied to the conductors via boundary conditions. In the plane of conductors, boundary conditions are satisfied at $N_d$ discrete points with $N_d$ being equal to the number of terms in the series expansions. The resulting system of inhomogeneous linear equations is solved by matrix inversion. No iterations are required. A discussion of the calculated line admittance parameters as functions of width of conductors, thickness of the layers, and frequency is given. The interconnect capacitance and conductance per unit length results are given and compared with those obtained using full wave solutions, and good agreement have been obtained in all the cases treated

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회A
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• pp.1322-1327
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• 2007

Many automotive companies have tried to apply the aluminum alloy sheet to car body because reducing the car weight can improve the fuel efficiency of vehicle. In order to do that, sheet materials require of weldablity, formability, productivity and so on. Aluminum alloy was not easy to join these metals due to its material properties. Thus, the laser is good heat source for aluminum alloy welding because of its high heat intensity. However, the welding quality was not good by porosity, underfill, and magnesium loss in welded metal for AA5182 aluminum alloy. In this study, Nd:YAG laser welding of AA 5182 with filler wire AA 5356 was carried out to overcome this problem. The weldability of AA5182 laser welding with AA5356 filler wire was investigated in terms of tensile strength and Erichsen ratio. For full penetration, mechanical properties were improved by filler wire. In order to optimize the process parameters, model to estimate tensile strength by artificial neural network was developed and fitness function was defined in consideration of weldability and productivity. Genetic algorithm was used to search the optimal point of laser power, welding speed, and wire feed rate.

• Computers and Concrete
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• 제16권5호
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• pp.669-682
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• 2015

Degradation of reinforced concrete (RC) structures due to chloride penetration followed by reinforcement corrosion has been a serious problem in civil engineering for many years. The numerical simulation methods at present are mainly finite element method (FEM) and finite difference method (FDM), which are based on mesh. Mesh generation in engineering takes a long time. In the present article, the numerical solution of chloride transport in concrete is analyzed using radial point interpolation method (RPIM) and element-free Galerkin (EFG). They are all meshless methods. RPIM utilizes radial polynomial basis, whereas EFG uses the moving least-square approximation. A Galerkin weak form on global is used to attain the discrete equation, and four different numerical examples are presented. MQ function and appropriate parameters have been proposed in RPIM. Numerical simulation results are compared with those obtained from the finite element method (FEM) and analytical solutions. Two case of chloride transport in full saturated and unsaturated concrete are analyzed to test the practical applicability and performance of the RPIM and EFG. A good agreement is obtained among RPIM, EFG, and the experimental data. It indicates that RPIM and EFG are reliable meshless methods for prediction of chloride concentration in concrete structures.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1998년도 제13차 학술회의논문집
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• pp.135-140
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• 1998

The concept of dissipativity and passivity are of interest to us from a theoretical as well as a practical point of view. It is well known that the Riccati equation is derived from the dissipation inequality which expresses the fact that the system is dissipative; the energy stored inside the system doesn't exceed the amount of supply which flows into the system. The pencil model is regarded as a representation based on behavioral approach introduced by J.C. Willems. It has first order in the internal variable and zeroth order in the external variable. In general, any matrix pencil is transformed into a canonical form which is consist of several kind of sub-pencils, One of them has row full rank for $^\forall S\;\in\;\mathds{C}\;\bigcup{\infty}$, we call it under-determined mode of the model. In our opinion, most important properties of dynamical system lay in the mode. According to the properties of canonical form for pencil, it is shown that the storage function which characterizes the dissipativity of the system can be written as a LMI for the under-determined mode, if the system doesn't include impulse mode.