• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.163-167
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• 2014

The average temperatures for year and winter season have been risen by $0.7{\circ}C$ and $1.4{\circ}C$, respectively, during the last 30 years. Recently abnormal climate phenomena occurred frequently results in severe loss of vegetable crops grown in Korea. Specially, Chinese cabbages grown in the southern area of Korea are often significantly affected by sudden cold waves during winter season before harvest. This experiment was conducted to find out a potential role of covering materials on the protection of frost damage of 'Bularm' chinese cabbage in the winter season. The lowest temperature was $-15.8^{\circ}C$ in non-covering, $-8.1^{\circ}C$ in the PE film covering and $-4.6^{\circ}C$ in the non-woven fabric covering with PE film, respectively. The cumulative times below $4.0^{\circ}C$ were 145.5 hours for the non-covering treatment, 94 hours in the PE film covering and 14.5 hours in the non-woven fabric covering with PE film, respectively. The symptoms of frost damage were severe at non-covering chinese cabbages compared to polyethylene film (PE) non-woven fabric with PE covering ones. Microscopic studies showed the normal anatomical structure of palisade and spongy tissue of cabbage leaves covered with non-woven fabric with PE film. Leaf cells, however, were slightly damaged in cabbages covered with PE film alone, and both palisade and spongy cells were were completely collapsed in uncovered cabbages. The result of this study suggests that chinese cabbages is required to be covered with non-woven fabric with PE film to minimize the frost damage by sudden cold wave below $-7^{\circ}C$.

• Journal of Korean Society of Forest Science
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• v.79 no.2
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• pp.138-143
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• 1990

In mature stand of Japanese larch (Larix leptolepis(S. et Z.) Gordon) planted in 1925, heartrot damage was investigated by felling some infected trees with fruit bodies of two butt-rot fungi, Phaeolus schweinitzii and Sparassis crispa. Percentage of heartrot area on basal area of decay trees was 19.2-74.4, and the height reached by the decay was 1.2-5.5m. Percentage of decay volume was in the range of 2.2-13.4%, but the loss wood to the height reached by the decay amounted to 27.2% (range 13.1-37.1%) of whole volume, Decay damage caused by S. crispa was much greater than that caused by P. schweinitzii. Compressive strength ($52-77kg/cm^2$) and specific gravity (0.40-0.47) of rot wood was reduced to 77% and 39% respectively in maximum as compared to sound wood of healthy tree, and those of sound-looking wood close by heartrot to 42% and 26%. The current annual growth of trees with decay was fallen behind that of sound tree from eight to fourteen years after planting in D. B. H. and from twelve to twenty-one years after in volume.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.203-207
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• 2008

The present study aimed at assessing the protective effect of water extract from fruit body of the Grifola frondosa (GFW) on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity. Rats orally administered with GFW 0.5, 1.0, 2.0 g/kg for 14 days were treated with $CCl_4$ to induce hepatotoxicity. Pretreatment with GFW remarkably prevented the elevation of serum AST, ALT, ALP, LDH, $\gamma$-GTP, and liver lipid peroxides in $CCl_4$-treated rat and GFW administration in liver injured rats by $CCl_4$ showed significant (p<0.05) protection of liver as evidenced from normal serum enzymes and malondialdehyde (MDA) levels. In the ultrastructural changes, administration of $CCl_4$-induced damage of hepatocytes with vacuolation, a highly damaged endoplasmic reticulum, and degenerating nuclei. However, pre-administration with GFW preserved normal ultrastructure of hepatocytes. These results suggest that GFW had an effect to inhibit $CCl_4$-induced liver injury in rat, and that it could be used as an effective hepatoprotective agent against chemical-induced liver damage.

• Korean Journal of Pharmacognosy
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• v.39 no.4
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• pp.335-340
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• 2008

Diabetes mellitus is metabolic disorder characterized by hyperglycemia caused by insufficient insulin secretion or insulin receptor insensitivity to endogenous insulin. It is well-known that hyperglycemia is one of the main causes of oxidative stress in both type 1 and 2 diabetes. Oxidative stress is related by death of pancreatic ${\beta}$ cell and dysfunction of ${\beta}$ cell. Although ${\beta}$ cell death or dysfunction is induced by many substances or molecules, increased evidences that oxidative stress plays a crucial role in ${\beta}$ cell death or dysfunction. Considering the importance of oxidative stress in the pathogenesis of diabetes mellitus, we investigated the cytoprotective effects against hydrogen peroxide-induced oxidative stress in pancreatic ${\beta}$ cell line RIN-m5F cell. 110 Plant sources were collected in Mt. Baek-du, and extracted with methanol. These extracts had been screened the protective effects against hydrogen peroxide-induced oxidative damage in RIN-m5F cells at 50 and 200 ${\mu}g$/ml. Of these, ten methanolic extracts, aerial part of Erigenron cannadensis, aerial part of Lespedeza juncea, whole plant of Alopecurus aequalis, fruit of Lycium chinense, leaf of Morus alba, rhizome of Polygonatum odoratum, root of Ampelosis japonica, whole plant of Ranunculus japonicus, aerial part of Polygonum sieboldii, rhizome of Arisaema amurense var. violaceum showed significant protective effects against hydrogen peroxide-induced oxidative damage in pancreatic ${\beta}$ cell line RIN-m5F cell.

• The Korean Journal of Physiology and Pharmacology
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• v.15 no.6
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• pp.353-361
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• 2011

In this study, cyanidin-3-glucoside (C3G) fraction extracted from the mulberry fruit (Morus alba L.) was investigated for its neuroprotective effects against oxygen-glucose deprivation (OGD) and glutamate-induced cell death in rat primary cortical neurons. Cell membrane damage and mitochondrial function were assessed by LDH release and MTT reduction assays, respectively. A time-course study of OGD-induced cell death of primary cortical neurons at 7 days in vitro (DIV) indicated that neuronal death was OGD duration-dependent. It was also demonstrated that OGD for 3.5 h resulted in approximately 50% cell death, as determined by the LDH release assay. Treatments with mulberry C3G fraction prevented membrane damage and preserved the mitochondrial function of the primary cortical neurons exposed to OGD for 3.5 h in a concentration-dependent manner. Glutamate-induced cell death was more pronounced in DIV-9 and DIV-11 cells than that in DIV-7 neurons, and an application of $50{\mu}M$ glutamate was shown to induce approximately 40% cell death in DIV-9 neurons. Interestingly, treatment with mulberry C3G fraction did not provide a protective effect against glutamate-induced cell death in primary cortical neurons. On the other hand, treatment with mulberry C3G fraction maintained the mitochondrial membrane potential (MMP) in primary cortical neurons exposed to OGD as assessed by the intensity of rhodamine-123 fluorescence. These results therefore suggest that the neuroprotective effects of mulberry C3G fraction are mediated by the maintenance of the MMP and mitochondrial function but not by attenuating glutamate-induced excitotoxicity in rat primary cortical neurons.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.2
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• pp.131-136
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• 2014

This study was carried out to determine the effects of wind speed on physiological responses in 'Fuji' apple (Malus pumila Miller). Two levels of wind blowing (3 and $5m{\cdot}s^{-1}$) were produced by large electric fans. Photosynthetic rate was reduced by one-way wind blowing treatment at $5m{\cdot}s^{-1}$, compared to the mild wind control, and this reduction was more obvious with stronger wind and increasing duration of wind application. The reduction in photosynthesis by the wind treatments was correlated with that in the proportion of opened stomates and stomatal conductance. The one-way wind treatment at $5m{\cdot}s^{-1}$ caused a leaf browning and leaf fall, and this negative effect became more serious with increasing time of exposure to the wind treatments. The sap flux through stem increased in all wind treatments compared to the natural mild wind.

• Journal of the Korea Convergence Society
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• v.12 no.12
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• pp.153-159
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• 2021

The purpose of this study is to determine the demage of tooth surface changes according to exposure time of commercially available green grape juice and pomegranate juice. Extracted healthy human premolar enamel surfaces were used. Control group immersed in phosphate-buffered saline (PBS) and 10 ml of commercially available green grape juice and pomegranate juice applied experimental group was divided into 7 groups. The pH of the experimental juice was measured, and the change and micrographics of the surface were confirmed through a Scanning Electron Microscope (SEM). It was found that the more the immersion time between the tooth surface and acid juice, such as damage to the tooth surface, has a greater effect on the surface damage. Based on the results of this study, it is necessary to reduce the number of drinking times and retention time in the oral cavity.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1267-1267
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• 2001

The existing fruit sorter has the method of tilting tray and extracting fruits by the action of solenoid or springs. In peaches, the most sort processing is supported by man because the sorter make fatal damage to peaches. In order to sustain commodity and quality of peach non-destructive, non-contact and real time based sorter was needed. This study was performed to develop peach sorter using near-infrared spectroscopy in real time and nondestructively. The prototype was developed to decrease internal and external damage of peach caused by the sorter, which had a way of extracting tray with it. To decrease positioning error of measuring sugar contents in peaches, fiber optic with two direction diverged was developed and attached to the prototype. The program for sorting and operating the prototype was developed using visual basic 6.0 language to measure several quality index such as chlorophyll, some defect, sugar contents. The all sorting result was saved to return farmers for being index of good quality production. Using the prototype, program and MLR(multiple linear regression) model, it was possible to estimate sugar content of peaches with the determination coefficient of 0.71 and SEC of 0.42bx using 16 wavelengths. The developed MLR model had determination coefficient of 0.69, and SEP of 0.49bx, it was better result than single point measurement of 1999's. The peach sweetness grading system based on NIR reflectance method, which consists of photodiode-array sensor, quartz-halogen lamp and fiber optic diverged two bundles for transmitting the light and detecting the reflected light, was developed and evaluated. It was possible to predict the soluble solid contents of peaches in real time and nondestructively using the system which had the accuracy of 91 percentage and the capacity of 7,200 peaches per an hour for grading 2 classes by sugar contents. Draining is one of important factors for production peaches having good qualities. The reason why one farm's product belows others could be estimated for bad draining, over-much nitrogen fertilizer, soil characteristics, etc. After this, the report saved by the peach grading system will have to be good materials to farmers for production high quality peaches. They could share the result or compare with others and diagnose their cultural practice.

• Journal of Environmental Science International
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• v.22 no.9
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• pp.1181-1186
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• 2013

Due to climate change, the occurrence of abnormal weather conditions has become more frequent, causing damage to vegetable crops grown in Korea. Hot pepper, Chinese cabbage and radish, the three most popular vegetables in Korea, are produced more in the field than in the greenhouse. It has been a trend that the time for field transplanting of seedlings is getting earlier and earlier as the spring temperatures keep rising. Seedlings transplanted too early in the spring take a longer time to resume the normal growth, because they are exposed to suboptimal temperature conditions. This study examined the influence of air temperature during seedling growth on the time required to reach the first fruit maturity and yield of hot pepper. Seedling plants of 'Super Manita' hot pepper was grown in temperatures $2.5^{\circ}C$ and $5.0^{\circ}C$ lower than the optimum temperature (determined by the average of temperatures for the past 5 years). Seedlings were transplanted into round plastic containers (30-cm diam., 45-cm height) and were placed in growth chambers in which the ambient temperature was controlled under natural sunlight. The growth of seedlings under lowered temperatures was reduced compared to the control. The mineral (K, Mg, P, N) concentrations in the leaf tissues were higher when plants were grown with the ambient temperature $2.5^{\circ}C$ lower than the optimum, regardless of changes in other growth parameters. Tissue calcium (Ca) concentration was the highest in the plants grown with optimum temperature. The carbohydrate to nitrogen (C/N) ratio, which was the highest (18.3) in the plants grown with optimum temperature, decreased concomitantly as the ambient temperature was lowered $2.5^{\circ}C$ and $5.0^{\circ}C$. The yield of the early harvested fruits was also reduced as the ambient temperature became lower. The first fruit harvest date for the plants grown with optimum temperature (June 27) was 13 days and 40 days, respectively, earlier than that in plants grown with $2.5^{\circ}C$ (July 10) and $5.0^{\circ}C$ (Aug 6) lower ambient temperatures. The fruit yield per plant for the optimum temperature (724 g) was the greatest compared to those grown with $2.5^{\circ}C$ (446 g) and $5.0^{\circ}C$ (236 g) lower temperatures. The result of this study suggests that the growers should be cautioned not to transplant their hot pepper seedlings too early into the field, since it may delay the time of first harvest eventually reducing total fruit yield.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.191-197
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• 2015

Fruit drop due to Eurytoma maslovskii infestations of Japanese apricot (Prunus mume) is a serious economic issue in most parts of Jeonnam Province, with the exception of the coastal areas such as Wando, Shinan, Yeosu, and Muan. The average incidence of fruit drop was 67% in 2013 and 33.3% in 2014. E. maslovskii larvae overwinter inside the pits of Japanese apricots. Larval survival rates decrease to less than 30% after a rainy summer season. The eggs are long oval of 0.68 mm, 0.29 mm and ivory white. Mature larva is of 6.56 mm, 3.18 mm. Free pupa are black, adult females and males of 6.97 mm, 4.90 mm lengths, respectively, while the ovipositor is 0.64 mm in length. Adult emergence occurs from early April to early May, when Japanese apricot fruits are from 4 mm to 17 mm in diameter. Adults persist for 13.5 d after emergence, and the sex ratio of females to males was found th be 45.9:54.1. Although up to four eggs may be oviposited per fruit, only one larva will ultimately survive, ans the larvae are cannibalistic. The period during which E. maslovskii is able to oviposit on Japanese apricots only lasts from mid- to late April (fruit diameter: 12~16 mm). When the diameter of the fruit is >16 mm, the pit of the Japanese apricot hardens and larvae have difficultly penetrating the stone. Therefore, the most effective method of controlling this pest is to spray orchards with a control agent 2~3 times, at 5 d intervals, beginning in mid- April.