• 한국균학회지
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• 제46권1호
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• pp.1-8
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• 2018

국내에 보고되지 않은 3종의 균류가 담수환경으로 부터 분리되었다. NNIBRFG108은 강원도 삼척의 담수침전식물체에서 분리되었고, NNIBRFG1139와 NNIBRFG1480은 경북 김천과 제주지역의 토양으로부터 분리되었다. 형태적 특징 및 internal transcribed spacers (ITS), 18S rDNA, 28S rDNA 및 ${\beta}$-tubulin 유전자를 이용한 분자계통학적 분류를 통해 동정한 결과, NNIBRFG108, NNIBRFG1139, NNIBRFG1480 균주는 각각 Didymella segeticola, D. ellipsoidea 및 D. aeria으로 확인되었다. 이는 국내에서는 발견되지 않은 미기록종으로 본 보고가 국내 최초이다.

• Mycobiology
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• 제28권4호
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• pp.197-201
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• 2000

The distribution and occurrence of aquatic zoosporic and terrestrial fungi were investigated in 21 well waters in Assiut governorate, Egypt. Using a zoospore capture technique, 923 colonies of aquatic freshwater fungi were recovered from well waters, out of which 811 colonies reached sexual maturity. These colonies were assigned to 23 species which belong to 11 genera. The most common genera were Achlya, Saprolegnia and Dictyuchus. Using two types of media, 35 species in addition to 2 varieties of terrestrial fungi which belong to 18 genera were also recovered. The most frequent glucophilic genera (recovered on glucose-Czapek's agar at $28^{\circ}C$) were Aspergillus, Penicillium and Fusarium. The results obtained on cellulose-Czapek's agar at $2^{\circ}C$ were basically similar to those on glucose agar and the most frequent genera were Aspergillus, Penicillium and Fusarium followed by Chaetomium and Cephalosporium.

• Mycobiology
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• 제46권3호
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• pp.205-214
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• 2018

The order Mucorales, the largest in number of species within the Mucoromycotina, comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of undiscovered taxa in Korea, two novel mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were isolated from specific habitats including freshwater and fecal samples, respectively. On the basis of their morphological characteristics and sequence analyses of internal transcribed spacer and large subunit ribosomal DNA, the CNUFC-GWD3-9 and CNUFC-EGF1-4 isolates were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively. It is ecologically, pathologically, and mycologically significant to find such rare zygomycetous fungi in such specific habitats.

• 한국균학회지
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• 제50권3호
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• pp.217-224
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• 2022

본 연구는 광주광역시와 전라남도 여수시의 담수환경으로부터 야생효모를 분리하여 특성 분석을 하였다. 분리된 효모의 동정을 위하여 26S rDNA의 D1/D2부위염기서열을이용하였다. 결과적으로 Apiotrichum chiropterorum (NNIBRFG36995), A. domesticum (NNIBRFG32938), A. dulcitum (NNIBRFG33144), A. laibachii(NNIBRFG36991), S. quercus (NNIBRFG33183), Tausonia pullulans (NNIBRFG33181)등 6종의 야생효모들을 동정하였다. 이들 6종의 효모는 국내에 기록된 바가 없으며, 본 논문이 최초보고이다. 미기록 효모 모두 PD, YM 배지에서 잘 자랐으며, 15-30℃ 온도 범위에서 생육하였다. A. domesticum (NNIBRFG32938), A. laibachii (NNIBRFG36991), Tausonia pullulans (NNIBRFG33181) 균주들은 20% glucose를 함유한 YPD 배지에서 생육하는 내당성을 보였으며A. domesticum (NNIBRFG32938)과 A. laibachii (NNIBRFG36991)는 5% NaCl을 함유한 YPD 배지에서도 생육하여 내염성을 가졌다.

• 한국균학회지
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• 제43권2호
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• pp.92-98
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• 2015

대표적인 담수습지인 경상남도 창원시 주남저수지와 동판저수지에서 우점하는 수생식물종인 자라풀 및 생이가래를 채집하였다. 주남저수지의 자생식물 뿌리에서 19균주와 동판저수지의 자생식물 뿌리에서 9균주를 순수분리 하였다. 이들 내생균류들의 internal transcribed spacer (ITS) 영역 염기서열을 분석하여 계통수를 작성한 결과 분리된 28균주는 주남저수지의 경우 11속, 동판저수지의 경우 5속에 속하는 것으로 확인되었으며, 두 습지에서 모두 13속의 내생균류가 분리되었다. 이들 중 담수습지별로 공통적으로 분리된 균주는 Fusarium, Phoma 및Talaromyces속으로 확인되었다. 담수습지 및 식물종별 내생균류의 다양성을 분석하였을 때 각각 상이한 지수를 보였으며, 그들 중 환경생태학적으로 중요한 위치를 차지하는 생이가래가 높은 지수를 나타내었다.

• Mycobiology
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• 제45권4호
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• pp.318-326
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• 2017

In a survey of undiscovered taxa in Korea, three zygomycete fungal strains-EML-W31, EML-HGD1-1, and EML-RUS1-1-were isolated from freshwater, grasshopper fecal, and soil samples in Korea. On the basis of the morphological characteristics and phylogenetic analysis of internal transcribed spacer and 28S rDNA, the isolates of EML-W31, EML-HGD1-1, and EML-RUS1-1 were confirmed to be Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans, respectively. These species have not been previously described in Korea.

• Mycobiology
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• 제46권4호
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• pp.317-327
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• 2018

While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.

• Mycobiology
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• 제48권5호
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• pp.410-417
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• 2020

Fungal ecological interactions play a key role in structuring community assemblages. These associations may involve both antagonistic and synergistic relationships, which are commonly influenced by abiotic factors such as nutrient conditions. However, information for extreme, oligotrophic systems remain poor. Herein, interactions among key members of the aquatic transient fungal community (Aspergillus niger, Cladosporium sp., and Coprinellus micaceus) of a low-nutrient freshwater system in the Cuatro Ci enegas Basin, Mexico were studied. Pairwise interaction bioassays were explored in vitro under different nutrient conditions, including carbohydrates-rich, carbohydrates and amino peptides-rich, and low nutrients. Our results indicated that antagonistic patterns prevail among the studied taxa. However, nutrient-dependent changes were observed in Cladosporium sp. shifting to synergy under carbohydrates-rich conditions, suggesting changes in the fungal community composition as a result of nutrient enrichment. Remarkably, our findings contrast with previous work demonstrating mainly synergistic interactions between our tested fungal isolates and co-occurring autochthonous bacteria (Aeromonas spp. and Vibrio sp.) under low-nutrient conditions. This observation may indicate that bacteria and fungi exhibit distinct community-level responses, driven by nutrient conditions. This contributes to the knowledge of fungal community dynamics and interspecific interactions in an oligotrophic ecosystem, highlighting the relevance of nutrient-based shifts and antagonistic interactions in ecosystem dynamics.

• Mycobiology
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• 제48권1호
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• pp.29-36
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• 2020

During an investigation of fungi of the order Mucorales from freshwater and sediment samples in Korea, we isolated six strains, NNIBRFG6649, NNIBRFG6255, NNIBRFG1498, CNUFC-YJ13, CNUFC-YR7, and NNIBRFG2739. The morphology and phylogeny of these strains were analyzed. Based on the morphological characteristics and molecular data from internal transcribed spacer (ITS) region, the isolates NNIBRFG6649 and NNIBRFG6255 were identified as Mucor abundans, and M. aligarensis, respectively. The isolates NNIBRFG1498 and CNUFC-YJ13 were identified as M. moelleri, whereas the isolates CNUFC-YR7 and NNIBRFG2739 were identified as M. heterogamus. To the best of our knowledge, M. abundans, M. aligarensis, M. moelleri, and M. heterogamus have not yet been reported in Korea.

• Mycobiology
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• 제45권4호
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• pp.344-352
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• 2017

During a survey of fungal diversity of the order Mucorales, three zygomycete isolates, CNUFC-YR113-1, CNUFC-KNU16-7, and CNUFC-BS1-1 were isolated from freshwater and soil samples in Korea. The strains were analyzed both morphologically and phylogenetically based on internal transcribed spacer and 28S rDNA gene sequences. Based on their morphology and phylogeny, the CNUFC-YR113-1 and CNUFC-KNU16-7 isolates were identified as Actinomucor elegans, and CNUFC-BS1-1 was identified as Mucor minutus. To the best of our knowledge, the species A. elegans and M. minutus, belonging to an undiscovered taxon, have not been previously described in Korea.