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Isolation and Characterization of Three Zygomycetous Fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus

  • Nguyen, Thuong T.T. (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
  • Received : 2018.04.27
  • Accepted : 2018.08.24
  • Published : 2018.12.31

Abstract

While surveying undiscovered fungal taxa in Korea, three rare zygomycetous fungal strains, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were isolated from soil, leaf, and freshwater samples, respectively. The strains were analyzed morphologically as well as phylogenetically based on the internal transcribed spacer region and 28S rDNA sequences. Sequence analysis of the two loci revealed that the isolates, CNUFC-PTF2-1, CNUFC-TF3-1, and CNUFC-ESAF3-1, were identified as Backusella circina, Circinella muscae, and Mucor ramosissimus, respectively. These species have not yet been previously described in Korea.

Keywords

References

  1. Hibbett DS, Binder M, Bischoff JF, et al. A higherlevel phylogenetic classification of the fungi. Mycol Res. 2007;111:509-547. https://doi.org/10.1016/j.mycres.2007.03.004
  2. Benny GL. The methods used by Dr. R. K. Benjamin, and other mycologists, to isolate Zygomycetes. Aliso. 2008;26:37-61. https://doi.org/10.5642/aliso.20082601.08
  3. Hoffmann K, Pawlowska J, Walther G, et al. The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies. Persoonia. 2013;30:57-76. https://doi.org/10.3767/003158513X666259
  4. Walther G, Pawlowska J, Alastruey-Izquierdo A, et al. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia. 2013;30:11-47. https://doi.org/10.3767/003158513X665070
  5. Ellis JJ, Hesseltine CW. Two new members of the Mucorales. Mycologia. 1969;61:863-872. https://doi.org/10.1080/00275514.1969.12018810
  6. Kirk PM. Nomenclatural novelties. Index Fungorum. 2012;11:1-1.
  7. Benny GL, Benjamin RK. Observations on Thamnidiaceae (Mucorales). New taxa, new combinations, and notes on selected species. Aliso. 1975;8:301-351. https://doi.org/10.5642/aliso.19750803.10
  8. de Souza JI, Marano AV, Pires-Zottarelli CLA, et al. A new species of Backusella (Mucorales) from a Cerrado reserve in Southeast Brazil. Mycol Prog. 2014;13:975-980.
  9. Lima DX, Voigt K, de Souza CAF, et al. Description of Backusella constricta sp. nov. (Mucorales, ex Zygomycota) from the Brazilian Atlantic Rainforest, including a key to species of Backusella. Phytotaxa. 2016;289:59-68. https://doi.org/10.11646/phytotaxa.289.1.4
  10. Wanasinghe DN, Phukhamsakda C, Hyde KD, et al. Fungal diversity notes 709-839: taxonomic and phylogenetic contributions to fungal taxa with an emphasis on fungi on Rosaceae. Fungal Divers. 2018;89:1-236. https://doi.org/10.1007/s13225-018-0395-7
  11. van Tieghem P, Le Monnier G. Recherches sur les Mucorinees. Ann Sci Nat. 1873;17:261-399.
  12. Hesseltine CW, Fennell DI. The genus Circinella. Mycologia. 1955;47:193-212. https://doi.org/10.1080/00275514.1955.12024444
  13. Hesseltine CW, Ellis JJ. Notes on Mucorales, especially Absidia. Mycologia. 1961;53:406-426. https://doi.org/10.1080/00275514.1961.12017970
  14. Faurel L, Schotter G. Notes mycologiques VI. Sur quelques champignons coprophiles d'Afrique Equatoriale. Cah La Maboke. 1965;3:123-133.
  15. Patil SD, Kale JC. A new species of Circinella van Tiegh. and Le Monn. Curr Sci. 1981;50:544-544.
  16. Arambarri AM, Cabello MN. Circinella lacrymispora sp. nov., a new mucoral isolated from Argentine soils. Mycotaxon. 1996;57:145-149.
  17. Gonzalez MC, Murueta-Figueroa N, Medina-Ortiz C, et al. New record of Circinella muscae from a hydrocarbon polluted sand beach of Tabasco, Mexico. Mycotaxon. 2010;113:111-117. https://doi.org/10.5248/113.111
  18. Zheng RY, Liu XY, Wang YN. Circinella (Mucorales, Mucoromycotina) from China. Mycotaxon. 2017;132:43-62. https://doi.org/10.5248/132.43
  19. Spatafora JW, Benny GL, Lazarus K, et al. A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia. 2016;108:1028-1046. https://doi.org/10.3852/16-042
  20. Benny GL, Humber RA, Voigt K. Zygomycetous fungi: Phylum Entomophthoromycota and subphyla Kickxellomycotina, Mortierellomycotina, Mucoromycotina, and Zoopagomycotina. In: McLaughlin DJ, Spatafora JW, editors. Mycota VII, Part A, Systematics and evolution. New York (NY): Springer-Verlag; 2014. p. 209-250.
  21. Schipper MAA. On Mucor circinelloides, Mucor racemosus and related species. Stud Mycol. 1976;12:1-40.
  22. Nguyen TT, Duong TT, Lee HB. Characterization of two new records of Mucoralean species isolated from gut of soldier fly larva in Korea. Mycobiology. 2016;44:310-313. https://doi.org/10.5941/MYCO.2016.44.4.310
  23. Nguyen TT, Jung HY, Lee YS, et al. Phylogenetic status of two undescribed zygomycete species from Korea: Actinomucor elegans and Mucor minutus. Mycobiology. 2017;45:344-352. https://doi.org/10.5941/MYCO.2017.45.4.344
  24. Souza CAFD, Lima DX, Gurgel LMS, et al. Coprophilous Mucorales (ex Zygomycota) from three areas in the semi-arid of Pernambuco, Brazil. Braz J Microbiol. 2017;48:79-86. https://doi.org/10.1016/j.bjm.2016.09.008
  25. Thompson DP, Eribo BE. Extracellular enzyme production by Rhizopus and Mucor species on solid media. Can J Microbiol. 1984;30:126-128. https://doi.org/10.1139/m84-020
  26. Alves MH, Campos-Takaki GM, Porto ALF, et al. Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease. Braz J Microbiol. 2002;33:325-330.
  27. de Souza PM, Bittencourt MLA, Caprara CC, et al. A biotechnology perspective of fungal proteases. Braz J Microbiol. 2015;46:337-346. https://doi.org/10.1590/S1517-838246220140359
  28. Ribes JA, Vanover-Sams CL, Baker DJ. Zygomycetes in human disease. Clin Microbiol Rev. 2000;13:236-301. https://doi.org/10.1128/CMR.13.2.236
  29. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego, CA, USA: Academic Press; 1990. p. 315-322.
  30. Lee HB. Molecular phylogenetic status of Korean strain of Podosphaera xanthii, a causal pathogen of powdery mildew on Japanese thistle (Cirsium japonicum) in Korea. J Microbiol. 2012;50:1075-1080. https://doi.org/10.1007/s12275-012-2618-z
  31. Thompson JD, Gibson TJ, Plewniak F, et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  32. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98.
  33. Tamura K, Stecher G, Peterson D, et al. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725-2729. https://doi.org/10.1093/molbev/mst197
  34. Lee YS, Jung HY, Lee HB, et al. National list of species of Korea Ascomycota, Glomeromycota, Zygomycota, Myxomycota, Oomycota.Korea: National Institute of Biological Resources Ministry of Environment; 2015.
  35. Nguyen TT, Choi YJ, Lee HB. Isolation and characterization of three unrecorded Zygomycete fungi in Korea: Cunninghamella bertholletiae, Cunninghamella echinulata, and Cunninghamella elegans. Mycobiology. 2017;45:318-326. https://doi.org/10.5941/MYCO.2017.45.4.318
  36. Schwarz P, Bretagne S, Gantier JC, et al. Molecular identification of Zygomycetes from culture and experimentally infected tissues. J Clin Microbiol. 2006;44:340-349. https://doi.org/10.1128/JCM.44.2.340-349.2006
  37. White MM, James TY, O’Donnell K, et al. Phylogeny of the Zygomycota based on nuclear ribosomal sequence data. Mycologia. 2006;98:872-884. https://doi.org/10.1080/15572536.2006.11832617
  38. Li GJ, Hyde HD, Zhao RL, et al. Fungal diversity notes 253-366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers. 2016;78:1-237. https://doi.org/10.1007/s13225-016-0366-9
  39. de Azevedo Santiago ALCM, de Souza-Motta CM. Isolation of Mucorales from processed maize (Zea mays L.) and screening for protease activity. Braz J Microbiol. 2008;39:698-700. https://doi.org/10.1590/S1517-83822008000400019
  40. Heidary M, Habibi Z. Microbial transformation of androst-4-ene-3,17-dione by three fungal species Absidia griseolla var. igachii, Circinella muscae and Trichoderma virens. J Mol Catalysis B Enzymatic. 2016;126:32-36. https://doi.org/10.1016/j.molcatb.2016.01.007
  41. Alvarez E, Cano J, Stchigel AM, et al. Two new species of Mucor from clinical samples. Med Mycol. 2011;49:62-72. https://doi.org/10.3109/13693786.2010.499521
  42. Sim-oes K, Dietrich SMC, Hahn MG, et al. Purification and characterization of a phytoalexin elicitor from spores of the saprobe Mucor ramosissimus. Rev Bras Bot. 2005;28:735-744. https://doi.org/10.1590/S0100-84042005000400008
  43. Marques MR, Buckeridge MS, Braga MR, et al. Characterization of an extracellular endopolygalacturonase from the saprobe Mucor ramosissimus Samutsevitsch and its action as trigger of defensive response in tropical plants. Mycopathologia. 2006;162:337-346. https://doi.org/10.1007/s11046-006-0064-3
  44. Ko HS, Taguchi H, Takizawa K, et al. The enzymatic approach of zygomycosis - causing Mucorales. Kor J Med Mycol. 2007;12:9-17.
  45. Satari B, Karimi K. Mucoralean fungi for sustainable production of bioethanol and biologically active molecules. Appl Microbiol Biotechnol. 2018;102:1097-1117. https://doi.org/10.1007/s00253-017-8691-9
  46. Schwarz P, Lortholary O, Dromer F, et al. Carbon assimilation profiles as a tool for identification of zygomycetes. J Clin Microbiol. 2007;45:1433-1439. https://doi.org/10.1128/JCM.02219-06

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