• Title/Summary/Keyword: Frequency of resistance

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Insight into Norfloxacin Resistance of Acinetobacter oleivorans DR1: Target Gene Mutation, Persister, and RNA-Seq Analyses

  • Kim, Jisun;Noh, Jaemin;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1293-1303
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    • 2013
  • Antibiotic resistance of soilborne Acinetobacter species has been poorly explored. In this study, norfloxacin resistance of a soil bacterium, Acinetobacter oleivorans DR1, was investigated. The frequencies of mutant appearance of all tested non-pathogenic Acinetobacter strains were lower than those of pathogenic strains under minimum inhibitory concentration (MIC). When the quinolone-resistance-determining region of the gyrA gene was examined, only one mutant (His78Asn) out of 10 resistant variants had a mutation. Whole transcriptome analysis using a RNA-Seq demonstrated that genes involved in SOS response and DNA repair were significantly up-regulated by norfloxacin. Determining the MICs of survival cells after norfloxacin treatment confirmed some of those cells were indeed persister cells. Ten colonies, randomly selected from among those that survived in the presence of norfloxacin, did not exhibit increased MIC. Thus, both the low mutation frequency of the target gene and SOS response under norfloxacin suggested that persister formation might contribute to the resistance of DR1 against norfloxacin. The persister frequency increased without a change in MIC when stationary phase cells, low growth rates conditions, and growth-deficient dnaJ mutant were used. Taken together, our comprehensive approach, which included mutational analysis of the target gene, persister formation assays, and RNA sequencing, indicated that DR1 survival when exposed to norfloxacin is related not only to target gene mutation but also to persister formation, possibly through up-regulation of the SOS response and DNA repair genes.

The Effect of Load Impedances on the Frequency Response of Pressure Propagation in the Pneumatic Transmission Line (기체 전달 관로에 있어서 압력 전파의 주파수 응답에 대한 부하 임피던스의 영향)

  • Yoon, S.J.;Son, B.J.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.6 no.4
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    • pp.344-353
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    • 1994
  • This study numerically analyzed the dynamic characteristics of the frequency response on the pneumatic transmission line with load impedances. The pressure transfer function is represented by the distributed parameter line model. To validate the mathematical approximations of Bessel function ratios, the results of frequency response in a blocked line were compared with those obtained by the Infinite-product, Brown's and Square-root approximations. Special emphasis was given to the frequency response characteristics on the pneumatic transmission line with load impedances. Computations were carried out for the wide range of parameters in terms of load capacitance ratio and load resistance ratio. The present results indicated that the theoretical model is capable of accurately predicting the frequency response characteristics for any configuration of a fluid transmission line.

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Microwave Properties of HTS Parallel-Plate Sapphire Resonators Designed for the Two-Resonance Mode Method (Two-Resonance Mode 방법용으로 제작된 고온초전도 평행판 사파이어 공진기의 마이크로파 특성)

  • Jung, Ho-Sang;Yang, W.I.;Lee, J.H.;Lee, Sang-Young
    • Progress in Superconductivity
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    • v.11 no.2
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    • pp.106-111
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    • 2010
  • Sapphire resonators with $YBa_2Cu_3O_{7-\delta}$ (YBCO) endplates have provided a way to realize extremely high quality factor due to the extremely low dielectric loss of sapphire and conductive loss of YBCO films, which enables to measure the low surface resistance of superconductor films at microwave frequencies. We present microwave properties of HTS sapphire resonators designed for measuring the surface resistance of HTS films at millimeter-wave frequencies by using the two-resonance mode dielectric resonator method. Despite enhanced surface resistance ($R_S$) of YBCO films due to the quadratic frequency dependence of the $R_S$, the unloaded quality factor ($Q_0$) of the $TE_{021}$ mode sapphire resonator still appears to be well above $1\;{\times}\;10^6$ at a mm-wave frequency of 38 GHz at 10 K. However, it appears that the $TE_{012}$ mode $Q_0$ is unexpectedly low despite that the corresponding resonance peak looks uncoupled with parasitic modes. We discuss possible reasons for the unexpected results using the surface resistance at the $TE_{021}$, $TE_{012}$, and $TE_{011}$ mode frequencies.

Effects of the 8-week Resistance Exercise on Body Composition, Serum Hormone Profiles and Feeding Patterns of Obese Females (8주간 저항성 운동이 비만 여성의 신체 성분, 혈청 호르몬 및 섭식에 미치는 영향)

  • Choi Sang Ho;Jo Min Wha;Shin Dong Soon
    • Journal of Nutrition and Health
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    • v.37 no.10
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    • pp.888-898
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    • 2004
  • This study was designed to investigate the effects of 8-week resistance exercise by using dumbell on the body com-position, serum hormone profiles and feeding patterns in young and healthy female students with over 30% of body fat, aged 22 years in Masan, Korea. The subjects were all six, performed successfully the entire exercise-program. Anthropometry, serum lipid and neurohormone profiles of subjects were analyzed at 0, 4 and 8 weeks after exercise. And also their food and nutrient intakes were self-reported by 3-day record and food frequency questionnaire. Triceps skinfold-thickness of the subject students significantly reduced after the exercise to 46% less than before (p < 0.002) . The serum concentrations of leptin, insulin and catecholamine appeared to decrease steadily, but not significantly. Total protein in serum increased significantly (p<0.05), and Na (p<0.005), K (p<0.001) and Fe (p<0.058) concentrations increased remarkably within the normal range. The 8-week resistance exercise tended to induce fiber consumption (p<0.089) of subject students and changed the P/M/S ratio changed into the desirable way from 0.8 : 1 : 1 to 1 : 1 : 1, and that of n-6/n-3 PUPA from 11 : 1 to 6 : 1. After taking exercise, the intake frequency of cookies (p<0.058) and raymeun (p<0.085) decreased, but finally this feeding pattern returned to the way as before even though at marginal significance. Consequently the resistance exercise may induce the desirable changes of body fat and improve serum lipid profiles and feeding patterns in young obese females.

The Analysis of a High Frequency Series Resonant DC-DC Converter (고주파 직렬공진형 DC-DC Converter의 특성 해석)

  • 이윤종;김철진
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.39 no.9
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    • pp.934-943
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    • 1990
  • There are no turn-on losses in the series Resonant Converter which operates above the resonance frequency, and the commutation stress on the switched component is low. For a given Series Resonant Converter with specified load resistance, the output voltage is a function of the operation frequency. This paper describes the static and dynamic characteristic analysis of the Series Resonant DC to DC Converter, which is operating above the resonant frequency, with frequency control. For the analysis method, state plane technique is adopted, and the circuit operation is defined from normalized switching frequency, Fsn. Under this condition, circuit performance is analyzed ideally. The validity of the proposed analysis is verified by comparing with experimental results, the stability of the converter is confirmed against small variations around the operating point by conventional frequency domain analysis, and the stress quantity added to switch component is shown.

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A Study on Filterless Frequency Doubler (휠터를 사용하지 않는 주파수 2체배기에 관한 연구)

  • 김경희
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.16 no.6
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    • pp.31-39
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    • 1979
  • This paper dealt with a general the Qrem of frequency doubler. By summarizing the frequency doubler theorems, a new frequency doubter theorem can be derived. "In time invarient system, if the input waveform shows the half-wave symmetric characteristic and if the even function is the mapping from input into output, then the frequency of output is at least 2-times of input frequency." By the theorem, this paper propose a new wide band square wave frequency doublerusing negative resistance circuit.e circuit.

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A Study on the Heat Transfer Enhancement of Miniature loop Heat Pipes by Using the Cu Nanofluids

  • Kim, Young-Sik;Jeong, Hyo-Min;Chung, Han-Shik;Tanshen, Md.Riyad;Lee, Dae-Chul;Ji, Myoung-Kuk;Bae, Kang-Youl
    • Journal of Power System Engineering
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    • v.17 no.2
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    • pp.70-77
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    • 2013
  • An experimental study was carried out to understand the heat transfer performance of a miniature loop heat pipes using water-based copper nanoparticles suspensions as the working fluid. The suspensions consisted of deionized water and copper nanoparticles with an average diameter of 80 nm. Effects of the cupper mass concentration and the operation pressure on the average evaporation and condensation heat transfer coefficients, the critical heat flux and the total heat resistance of the mLHPs were investigated and discussed. The pressure frequency also depends upon the evaporator temperature which has been maintained from $60^{\circ}C$ to $90^{\circ}C$. The Investigation shows 60% filling ratio gives the highest inside pressure magnitude of highest number pressure frequency at any of setting of evaporator temperature and 5wt% results the lowest heat flow resistance.

Responses of Peach Blossom Blight and Brown Rot Fungus Monilinia fructicola to Benzimidazole and Diethofencarb in Korea

  • Lim, Tae-Heon;Kim, Jin-Ho;Cha, Byeong-Jin
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.1-6
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    • 2006
  • The population shifts of Monilinia fructicola isolates which were resistant to the fungicide benzimidazoles were investigated in four regions of Korea from 1998 to 2000. The isolation frequency of benzimidazole-resistant isolates ranged from 18.8% to 29.6% in Chongdo and from $22.0\%$ to $26.8\%$ in Gyeongsan during the same period. However, the frequency of benzimidazoleresistant isolates was less than $4.0\%$ in Chochiwon and Youngduk during the same period. Benzimidazoleresistant isolates showed cross-resistance among benzimidazoles. On the other hand, none of the isolates showed cross-resistance to diethofencarb and carbendazim. Regardless of the year, the benzimidazole-resistant isolates of $EC_{50}$ higher than 500 $\mug%$ a.i./ml were isolated more frequently in mid and late season than in early season. In an orchard of Gyeongsan that had not been exposed to any fungicides for several years, the population of benzimidazole-resistant isolate had persisted without much fluctuation for three years. These results suggest that benzimidazole resistance of M. fructicola is becoming a problem in controlling brown rot and blossom blight of peach in regions like Chongdo and Gyeongsan.

A Study on Degradation Characteristic of High Strength Fire Resistance Steel for Frame Structure by Acoustic Emission (음향방출법에 의한 고강도 구조요 내화강의 열화특성에 관한 연구)

  • 김현수;남기우;강창룡
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2000.04a
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    • pp.51-56
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    • 2000
  • Demand for new nondestructive evaluations is growing to detect tensile crack growth behavior to predict long term performance of materials and structure in aggressive environments, especially when they are in non-visible area. Acoustic emission technique is well suited to these problems and has drawn a keen interests because of its dynamic detection ability, extreme sensitivity and location of growing defects. In this study, we analysed acoustic emission signals obtained in tensile test of high strength fire resistance steel for frame structure with time frequency analysis methods. The results obtained are summaries as follows ; In the T and TN specimen consisting of ferrite and pearlite grains, most of acoustic emission events were produced near yield point, mainly due to the dislocation activities during the deformation. However, B specimen under $600^{\circ}C$ - 10min had a two peak which was attribute to the presence of martensite phase. The first peak is before yield point the second is after yield point. The sources of second acoustic emission peak were the debonding of martensite-martensite interface and the micro-cracking of brittle martensite phase. In $600^{\circ}C$-30min to $700^{\circ}C$-60min specimens, many signals were observed from area before yield point and counts were decreased after yield point.

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Electrical Characteristics Assessment for PE Series Insulations (PE 계열 절연재 전기적 특성 평가)

  • Jung, Jong-Wook;Jung, Jin-Soo;Han, Woon-Ki
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.5
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    • pp.430-435
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    • 2007
  • This paper describes the results of electrical characteristics assessment for organic insulations of polyethylene(PE) series insulations and acrylonitrile butadiene styrene copolymer(ABS). In the experiment, 4 kinds of specimens by composition density were tested in relative permittivity, specific resistance and tracking duration. A WinDETA system and a tracking test set manufactured for this assessment were used to measure the dielectric parameters and tracking duration, respectively. In measuring the tracking duration, the time from testing voltage application to testing circuit breaking due to the tracking current was measured. As a result, dielectric dispersion was observed in measuring the relative permittivity of ABS. It was confirmed that the relative permittivity decreased with the density of the PE series insulations and it depends rather on the temperature than frequency. In most specimens, specific resistance exponentially decreased with frequency and the result for each specimen was almost similar. By the way, in the tracking test, all the PE series insulations showed more excellent performance than ABS and especially in the case of HDPE, its tracking withstand performance was the best.