• International journal of advanced smart convergence
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• v.6 no.2
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• pp.59-64
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• 2017

There are three sorts of voices of female vocalists: soprano, mezzo-soprano, and contralto according to the transliteration. Among them, the soprano has the highest vocal range. Since the voice is generated through the human vocal tract based on the voice generation model, it is greatly influenced by the vocal tract. The structure of vocal organs differs from person to person, and the formants characteristic of vocalization differ accordingly. The formant characteristic refers to a characteristic in which a specific frequency band appears distinctly due to resonance occurring in each vocal tract in the vocal process. Formant characteristics include personality that occurs in the throat, jaw, lips, and teeth, as well as phonological properties of phonemes. The first formant is the throat, the second formant is the jaw, the third formant and the fourth formant are caused by the resonance phenomenon in the lips and the teeth. Among them, pronunciation is influenced not only by phonological information but also by jaws, lips and teeth. When the mouth is small or the jaw is stiff when pronouncing, pronunciation becomes unclear. Therefore, the higher the accuracy of the pronunciation characteristics, the more clearly the formant characteristics appear in the grammar spectrum. However, many soprano singers can not open their mouths because their jaws, lips, teeth, and facial muscles are rigid to maintain high tones when singing, which makes the pronunciation unclear and thus the formant characteristics become unclear. In this paper, in order to confirm the accuracy of the pronunciation characteristics of soprano singers, the experimental group was selected as the soprano singers A, B, C, D, E of Korea and analyzed the grammar spectrum and conducted the MOS test for pronunciation recognition. As a result, soprano singer B showed a clear recognition from F1 to F5 and MOS test result showed the highest recognition rate with 4.6 points. Soprano singers A, C, and D appear from F1 to F3, but it was difficult to find formants above 2kHz. Finally, the soprano singer E had difficulty in finding the formant as a whole, and MOS test showed the lowest recognition rate at 2.1 points. Therefore, we confirmed that the soprano singer B, which exhibits the most distinct formant characteristics in the grammar spectrum, has the best pronunciation accuracy.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.11 no.2
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• pp.221-232
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• 2016

The impact of the recent small business start-up competition in the market, has become overheated. It is effected by early retirement of a generation of youth employment. This study is a study on the impact of SEMAS(Small Enterprise and Market Service) system operating of the funding system, education support programs, and consulting support system on the business performance of small business owners. It has surveyed 272 business owners, in Gyeongsangnam-province. The study includes specific support system for usage frequency and satisfaction and conducted from January 2013 to September 2015. In addition, it analyzes characteristic that motivation, business model, item, owner's experience, sales and demographic by small business owner. Analysis results, the management performance of small business that uses financial support system and consulting support system is shown to be high. But education support system is the opposite effect. As a result, the management performance is related to industry experience. Therefore education support system need to be reorganized to the support depends on the development stage. This study was conducted to help small business owners entered the start-up market and a decision-making person with a policy decision.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.89-96
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• 2017

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

• Journal of Broadcast Engineering
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• v.20 no.1
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• pp.26-39
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• 2015

UHD (Ultra High Definition) has been developed and standardized over the past few years and considered as a key of the next generation broadcasting. Since major sporting events that attract many viewers have been good opportunities for applying and promoting new broadcasting technology, KBS (Korean Broadcasting System) has carried out live 4K terrestrial broadcasting of three big sports events in 2014, the KBL (Korean Basketball League) finals, the 2014 FIFA World Cup, and the 2014 Incheon Asian Games. Especially, 4K UHD live coverage of the KBL finals was the world's first live 4K terrestrial broadcasting, and people could enjoy the World Cup and the Asian Games with UHDTV at home. In this paper, the overall live production and data transmission of the sporting events is described, including on-location live production, live HEVC (High Efficiency Video Coding) encoding, SFN (Single Frequency Network) transmission, as well as experimental IP transmission of uncompressed 4K video via KREONET, a national R&D network run by KISTI (Korea Institute of Science and Technology Information).

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.6
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• pp.286-304
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• 2017

In this study, we proposed a new-type of circular perforated caisson breakwater consisting of a bundle of latticed blocks that can be applied to a small port such as a fishing port, and numerically investigated the hydraulic characteristics of the breakwater. The numerical method used in this study is OLAFOAM which newly added wave generation module, porous media analysis module and reflected wave control module based on OpenFOAM that is open source CFD software published under the GPL license. To investigate the applicability of OLAFOAM, the variations of wave pressure acting on the three-dimensional slit caisson were compared to the previous experimental results under the regular wave conditions, and then the performance for irregular waves was examined from the reproducibility of the target irregular waves and frequency spectrum analysis. As a result, a series of numerical simulations for the new-type of circular perforated caisson breakwaters, which is similar to slit caisson breakwater, was carried out under the irregular wave actions. The hydraulic characteristics of the breakwater such as wave overtopping, reflection, and wave pressure distribution were carefully investigated respect to the significant wave height and period, the wave chamber width, and the interconnectivity between them. The numerical results revealed that the wave pressure acting on the new-type of circular perforated caisson breakwaters was considerably smaller than the result of the impermeable vertical wall computed by the Goda equation. Also, the reflection of the new-type caisson breakwater was similar to the variation range of the reflection coefficient of the existing slit caisson breakwater.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.188.2-188.2
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• 2015

The presence of the conduction interface in epitaxial $LaAlO_3/SrTiO_3$ thin films has opened up challenging applications which can be expanded to next-generation nano-electronics. The metallic conduction path is associated with two adjacent insulating materials. Such device structure is applicable to frequency-dependent impedance spectroscopy. Impedance spectroscopy allows for simultaneous measurement of resistivity and dielectric constants, systematic identification of the underlying electrical origins, and the estimation of the electrical homogeneity in the corresponding electrical origins. Such unique capability is combined with the intentional control on the interface composition composed of $SrTiO_3$ and $CaTiO_3$, which can be denoted by $SrxCa1-_xTiO_3$. The underlying $Sr_xCa1-_xTiO_3$ interface was deposited using pulsed-laser deposition, followed by the epitaxial $LaAlO_3$ thin films. The platinum electrodes were constructed using metal shadow masks, in order to accommodate 2-point electrode configuration. Impedance spectroscopy was performed as the function of the relative ratio of Sr to Ca. The respective impedance spectra were analyzed in terms of the equivalent circuit models. Furthermore, the impedance spectra were monitored as a function of temperature. The ac-based characterization in the 2-dimensional conduction path supplements the dc-based electrical analysis. The artificial manipulation of the interface composition will be discussed towards the electrical application of 2-dimensional materials to the semiconductor devices in replacement for the current Si-based devices.

• Journal of Life Science
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• v.16 no.3 s.76
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• pp.540-545
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• 2006

T-DNA-mediated transformation is a common method for generating transgenic plants with insertional mutagenesis. In order to identify important genes involved in shoot development, a system of promoter trap insertional mutagenesis was employed in Arabidopsis thaliana. For this system, an efficient promoter trap vector, pFGL561 was developed. The pFGL561 includes a basta-resistant gene, an intron with multiple splicing donor and acceptor sites, and a promoter-less GFP reporter gene. Using floral-dipping method, we made total 300 $T_1$ promoter-trap lines which were screened for GFP expression. GFP signals in the $T_1$ plants were detected with high frequency, 26.7%, and the signals were reconfirmed in $T_2$ plants. To isolate the genes that are involved in shoot development, phenotypes were analyzed in $T_2$ plants of the 19 $T_1$ lines that had GFP signals in shoot apex, and 6 $T_1$ lines were selected that had abnormal shoot development. These lines will be very useful for the investigation of shoot development.

• Journal of Technologic Dentistry
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• v.32 no.1
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• pp.25-34
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• 2010

This study was the study verifying dental technicians' motivation and job satisfaction in Seoul through the positive method as well as its purposes were to investigated the effect of dental technicians manpower supply/demand prospect on motivation, job satisfaction and the effect of other job satisfaction factors on dental technicians motivation and job satisfaction. For then, general characteristics of study objects were classified by sex, married/unmarried, age, career, salary, highest level of education, working place, position and job as well as job satisfaction factors were classified with future supply/demand prospect, future prospect, working hours, working environment and salary. We conducted a frequency analysis, crossing analysis and multi-linear regression analysis about the effect of the classified articles on motivation and job satisfaction. Also, for verifying the relationship of motivation and job satisfaction, we conducted correlation analysis and its result is as followed. First, it showed that the job related with making orthodontic appliance influenced on motivation, among the age, career and job of the general characteristics, and other jobs between working place and job influenced on job satisfaction. Second, the dark future prospect was showed to influence on motivation and job satisfaction highly so we could grasp that negative opinions are included in that. And low-paying was showed to highly influence on job satisfaction s fall so we could grasp the subjective low-salary standard at the present. But we expected that the manpower supply/demand prospect would influence on motivation and job satisfaction but there was little influence. Third, the correlation between motivation and job satisfaction was showed to be somewhat high, motivation had the standard which is above average and job satisfaction had the standard which is below the average. So with a little improving of job satisfaction, it s possible that motivation can be very high. Therefore, in rapidly changing generation, we think that the dental technicians are actively coping with the reality of low-paying and high-working. But it's implying that anxiety for uncertain future was reflected. Furthermore, because institutional strategies for dental technicians' efficient management are insufficient in relation with policies so it's necessary the policy consideration for solve the job-unsatisfying factors actively.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.7
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• pp.739-747
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• 2007

Dual-mode bandpass filter was designed by using periodic stepped impedance ring resonator with step perturbation. The periodic stepped impedance ring resonator has the effects of size reduction and $2^{nd}$ harmonic suppression by changing characteristic impedance ratio. The perturbation for dual-mode generation was also easily controlled by characteristic impedance ratio, and the variation of dual-mode resonant frequencies and attenuation pole frequencies were analyzed by the effect of step perturbation. Chip capacitors were used for input/output coupling, and the variation of center frequency by the coupling capacitance and step perturbation was also considered. From the results, two types of 2 GHz dual-mode bandpass filter were fabricated in size of $14{\times}14mm^2$, those have different attenuation poles and bandwidths. The measured results of proposed bandpass filters showed a good agreement with the calculated estimations, and those have insertion loss of 2.52, 0.52 dB and 3 dB bandwidth of 4.03, 15.02 %, respectively.

• Information Systems Review
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• v.8 no.1
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• pp.81-99
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• 2006

Recently, the area of ubiquitous has gained a great attention both in academics and in business organizations as we are entering an ubiquitous society where people can interact at "anytime" and "anywhere". To preoccupy the leading status for the next generation of IT technology, many countries such as Korea, Japan, and US are concerned the ubiquitous. In this tendency, companies want to identify what factors make people use ubiquitous in order to develop the effective ubiquitous service and to supply the higher quality service. However, prior researches on ubiquitous are focused on technical area such as higher data delivery, efficient compression of images, so they do not have given companies beneficial materials for making fascinating ubiquitous services. To offer the service materials for development, from the viewpoint of the customer, important factors to use ubiquitous services are investigated in this study. Moreover, the study was progressed up to the new area of investigation how much these factors influenced ubiquitous service actual usage. To achieve these goals, Ubiquitous service adopting reflected in ubiquitous service characteristics was suggested in this study. The results of this study are as follows. First, contextual offer, ubiquity, invisibility are positively related to perceived value. Second, perceived value is positively related to usage intention but perceived risk is negatively related to usage intention. Third, usage intention is positively related to usage frequency. Based on these results, managerial implications for ubiquitous service vitalization are discussed. Lastly, the limitation of this research and further research issues are suggested.