• Title/Summary/Keyword: Freezing temperature

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Studies on the technique of cultivating Gastrodia elata using small diameter log (참나무 소경목(小徑木)을 이용한 천마재배기술 연구)

  • Jo, Woo-Sik;Lee, Seonghak;Choi, Herim;Sul, Pilgeom;Lee, Suk-Hee;Son, Jin Wook;Lim, Myeong Ho
    • Journal of Mushroom
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    • v.15 no.2
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    • pp.69-72
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    • 2017
  • Diversification to develop oak pruned neck utilization technology reduction of operation cost of cheonma(Gastrodia elata) farmers is the purpose of this research, but the results of the experiment are as follows. The soil chemistry of the plantation was very weak in 2015 when the pH was 5.7, weakly acidic and EC was 0.41 (ds/m), but the faux spring area was very sunny, but at the end of May, early June, late July And so on were at a maximum temperature of $25^{\circ}C$ or more, and underground temperature fell below freezing in late January 2016. In the content of the survey of the harvested volume, treatment with a diameter of 6 to 10 cm appeared in 1,366 g total weight and in treated plots with a diameter of 20 cm or more, appeared in 1,542 g, confirming that the number and weight of the interspaces from the small neck is higher than the practice.

Study on Characteristics of Snowfall and Snow Crystal Habits in the ESSAY (Experiment on Snow Storms At Yeongdong) Campaign in 2014 (2014년 대설관측실험(Experiment on Snow Storms At Yeongdong: ESSAY)기간 강설 및 눈결정 특성분석)

  • Seo, Won-Seok;Eun, Seung-Hee;Kim, Byung-Gon;Ko, A-Reum;Seong, Dae-Kyeong;Lee, Gyu-Min;Jeon, Hye-Rim;Han, Sang-Ok;Park, Young-San
    • Atmosphere
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    • v.25 no.2
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    • pp.261-270
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    • 2015
  • Characteristics of snowfall and snow crystal habits have been investigated in the campaign of Experiment on Snow Storms At Yeongdong (ESSAY) using radiosonde soundings, Global Navigation Satellite System (GNSS), and a digital camera with a magnifier for taking a photograph of snowfall crystals. The analysis period is 6 to 14 February 2014, when the accumulated snowfall amount is 192.8 cm with the longest snowfall duration of 9 days. The synoptic situations are similar to those of the previous studies such as the Low pressure system passing by the far South of the Korean peninsula along with the Siberian High extending to northern Japan, which eventually results in the northeasterly or easterly flows and the long-lasting snowfall episodes in the Yeongdong region. In general, the ice clouds tended to exist below around 2~3 km with the consistent easterly flows, and the winds shifted to northerly~northwesterly above the clouds layer. The snow crystal habits observed in the ESSAY campaign were mainly dendrite, consisting of 70% of the entire habits. The rimed habits were frequently captured when two-layered clouds were observed, probably through the process of freezing of super-cooled droplets on the ice particles. The homogeneous habit such as dendrite was shown in case of shallow clouds with its thickness of below 500 m whereas various habits were captured such as dendrites, rimed dendrites, aggregates of dendrites, plates, rimed plates, etc in the thick cloud with its thickness greater than 1.5 km. The dendrites appeared to be dominant in the condition of cloud top temperature specifically ranging $-12{\sim}-16^{\circ}C$. However, the association of snow crystal habits with temperature and super-saturation in the cloud could not be examined in the current study. Better understandings of characteristics of snow crystal habits would contribute to preventing breakdown accidents such as a greenhouse destruction and collapse of a temporary building due to heavy snowfall, and traffic accidents due to snow-slippery road condition, providing a higher-level weather information of snow quality for skiers participating in the winter sports, and estimating more accurate snowfall amount, location, and duration with the fallspeed of solid precipitation.

Evaluation of Commercially Available Passive Samplers and Development of New Passive Samplers Part 2 : Development of New Passive Samplers (공기중 유기용제 농도 측정에 있어서 수동식 시료채취기의 성능평가 및 한국산 수동식 시료채취기의 개발에 관한 연구 제 2 부 : 한국산 수동식 시료채취기의 개발)

  • Paik, Nam Won;Kong, Sang Hui;Park, Jeong Im;Lee, Young Hwan
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.6 no.1
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    • pp.97-108
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    • 1996
  • A new type of passive samplers were designed and produced by authors. After evaluating the quality of activated carbon by measuring recovery rate of organic vapors and steadiness of sampling rate, activated carbon with 30 - 35 mesh produced by Company S in Korea was selected. In each passive sampler, an amount of 400 mg of the activated carbon was filled in 25-mm cassette and covered by fixed screen (or wire screen with 100 mesh). In addition to the fixed screen, a wind screen (or wire screen with 300 mesh) was also attached at outer face. The sampling rate of the new Korean passive samplers was estimated Conclusions obtained in the study are as follows. 1. Sampling rates of the newly developed Korean passive samplers were affected by sampling time. For n-hexane, sampling rates of 15- and 60-minute samples were 70.92 and 37.45 ml/min, respectively. Sampling rate of both 200- and 450-minute samples was 25.96 ml/min. It is concluded that, when passive samplers are used for measuring organic vapors, samples be collected longer than 60 minutes. 2. Sampling rate of the passive samplers was also affected by airborne concentration of organic vapors. Lower sampling rates were determined at level of 1/2 threshold limit values (TLVs) recommended by the American Conference of Governmental Industrial Hygienists (ACGIH). It is recommended that sampling rate of the passive samplers be obtained at site by measuring concentrations using both the NIOSH Method and passive samplers simultaneously. 3. When the passive samplers, which collected organic vapors, were exposed to clean air for five hours, there was no significant loss of organic vapors due to reverse diffusion. 4. When samples were stored at room temperature ($21.8{\pm}0.7^{\circ}C$) and refrigerator ($3.8{\pm}0.7^{\circ}C$), there was no significant difference in the accuracy of results. For trichloroethylene and n-hexane, accuracies were within 25 % at both temperatures until seven days. However, poor accuracy exceeding 25 % was indicated in toluene from the first day. It is recommended that samples be stored at freezing temperature below $0^{\circ}C$. 5. Sampling efficiency was significantly affected by direction of the passive samplers. Results of samplers facing wind and down, respectively, were compared. Lower amount of organic vapors were collected when the sampler was oriented down. It is recommended that, when air velocity is low in plants, the passive samplers be oriented to the wind. However, when air velocity is high, the passive samplers be oriented down.

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Preanalytical Stability of Adrenocorticotropic Hormone Depends on Time to Centrifugation (전혈에서 시간과 온도에 따른 Adrenocorticotropic Hormone의 안정성 평가)

  • Shin, Sun-Young;Lee, Hyun-Joo;Min, Gyung-Sun;Lee, Ho-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.1
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    • pp.116-119
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    • 2009
  • Background: Preanalytical factors can affect reliability of hormone assay results. Adrenocorticotropic hormone (ACTH) in blood is considered highly unstable because of proteolytic degradation, so storage of blood samples on ice until analysis is recommended. In clinical practice, however, this procedure may present logistical problems because most samples for ACTH measurement must be shipped from the place of sample collection to the laboratory. Therefore, we studied the impact of time and temperature before plasma separation and analysis on the results of ACTH assays. Methods: A total number of 22 patients were enrolled in this study. We obtained 2 blood samples. ACTH concentrations were 35~126 pg/mL. ACTH concentrations were measured by immunoradiometric assay (IRMA) using commercial kits (CIS Biointernational, Gif-sur-Yvette, France). Results: ACTH levels showed a significant difference between the samples of $22^{\circ}C$ EDTA and $4^{\circ}C$ EDTA. Measured ACTH concentrations significantly decreased with time before freezing at $-20^{\circ}C$. ACTH levels showed no significant difference between the groups of after storage for 24 hr without centrifugation at $22^{\circ}C$ and $4^{\circ}C$. Conclusion: We recommend that blood samples be obtained on pre-chilled EDTA collection tubes. The shortest possible time between sample collection and processing is always the best laboratory practice.

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Variation in Characteristics of Elastic Waves in Frozen Soils According to Degree of Saturation (포화도에 따른 동결토의 탄성파 특성 변화)

  • Park, Jung-Hee;Kang, Min-Gu;Lee, Jong-Sub
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.3
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    • pp.1063-1075
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    • 2013
  • The strength of frozen soils is one of the significant design parameters for the construction in frozen ground. The properties of frozen soils should be investigated to understand the strength of frozen soils. The objective of this study is to figure out the characteristics of elastic waves in frozen soils, which reflect the constituent and physical structure of frozen soils in order to provide fundamental information of those according to the degree of saturation. Freezing cell is manufactured to freeze specimens, which are prepared with the degree of saturation of 10%, 40%, and 100%. Piezo disk elements are used as the compressional wave transducers and Bender elements are used as the shear wave transducers. While the temperature of specimens changes from $20^{\circ}C$ to $-10^{\circ}C$, the velocities, resonant frequencies and amplitudes of the compressional and shear waves are investigated based on the elastic wave signatures. Experimental results reveal that the elastic wave velocities increase as the degree of saturation increases. The variation of resonant frequencies coincide with that of elastic wave velocities. A marked discrepancy in amplitudes of compressional and shear waves are observed at the temperature of $0^{\circ}C$. This study renders the basic information of elastic waves in frozen soils according the degree of saturation.

Cellular and Molecular Pathology of Fungi on Plants Studied by Modern Electron Microscopy

  • Sanwald, Sigrun-Hippe
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1995.06b
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    • pp.27-53
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    • 1995
  • In plant pathology there is an increasing necessity for improved cytological techniques as basis for the localization of cellular substances within the dynamic fine structure of the host-(plant)-pathogen-interaction. Low temperature (LT) preparation techniques (shock freezing, freeze substitution, LT embedding) are now successfully applied in plant pathology. They are regarded as important tools to stabilize the dynamic plant-pathogen-interaction as it exists under physiological conditions. - The main advantage of LT techniques versus conventional chemical fixation is seen in the maintenance of the hydration shell of molecules and macromolecular structures. This results in an improved fine structural preservation and in a superior retention of the antigenicity of proteins. - A well defined ultrastructure of small, fungal organisms and large biological samples such as plant material and as well as the plant-pathogen (fungus) infection sites are presented. The mesophyll tissue of Arabidopsis thaliana is characterized by homogeneously structured cytoplasm closely attached to the cell wall. From analyses of the compatible interaction between Erysiphe graminis f. sp. hordei on barley (Hordeum vulgare), various steps in the infection sequence can be identified. Infection sites of powdery mildew on primary leaves of barley are analysed with regard to the fine structural preservation of the haustoria. The presentation s focussed on the ultrastructure of the extrahaustorial matrix and the extrahaustorial membrane. - The integration of improved cellular preservation with a molecular analysis of the infected host cell is achieved by the application of secondary probing techniques, i.e. immunocytochemistry. Recent data on the characterization of freeze substituted powdery mildew and urst infected plant tissue by immunogold methodology are described with special emphasis on the localization of THRGP-like (threonine-hydrxyproline-rich glycoprotein) epitopes. Infection sites of powdery mildew on barley, stem rust as well as leaf rust (Puccinia recondita) on primary leaves of wheat were probed with a polyclonal antiserum to maize THRGP. Cross-reactivity with the anti-THRGP antiserum was observed over the extrahaustorial matrix of the both compatible and incompatible plant-pathogen interactions. The highly localized accumulation of THRGP-like epitopes at the extrahaustorial host-pathogen interface suggests the involvement of structural, interfacial proteins during the infection of monocotyledonous plants by obligate, biotrophic fungi.

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The Comparison of Growth and Yield Characteristics among Principal Bulb Onion (Allium Cepa L.) Cultivars in Organic Growing (양파 유기재배에서 주요 품종간 생육 및 수량특성 비교)

  • Min, Byeong-Gyu;Lee, Sun-Young;Moon, Jin-Seong;Ha, In-Jong;Hwang, Hae-Jun;Lim, Chae-Sin
    • Korean Journal of Organic Agriculture
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    • v.23 no.1
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    • pp.43-58
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    • 2015
  • This study is conducted in order to select the varieties that can supplement problems related with organic cultivation of bulb onion (Allium cepa L.). 15 test varieties were selected considering rural preference, breeding line, breeding company, open-pollinated cultivar, F1 hybrid cultivar of either domestic or foreign products. Onion varieties were assessed in 5 criteria which are : growth of plants in low temperature, freezing injury resistance, resistance to physiological disorder such as bolting and doubled bulb, lodging index during harvest, and marketable yield. As a result, e-Joeun, Katamaru, Pop, Art, Singihan among F1 hybrid cultivars and Changnyeongdaego, Seouldaego, and Chunjujunggo among open-pollinated cultivars were selected as more suitable varieties for the organic cultivation. Although Sunpower cannot be selected in varieties those are suitable for organic cultivation, it showed excellent traits in the bulb characteristics. No significant differences were found between open-pollinated cultivar and F1 hybrid cultivar except growth of plants in low temperature (plant sheath diameter) and lodging index during harvest (May 24th). And no significant differences were recognized between domestic varieties and foreign varieties except missing plant ratio after wintering.

Quality Changes in Minced Ginger Prepared with Frozen Ginger during Storage (냉동생강으로 제조한 다대기의 저장 중 품질변화)

  • Kim, Dong-Ho;Lee, Young-Chun
    • Korean Journal of Food Science and Technology
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    • v.36 no.6
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    • pp.943-951
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    • 2004
  • As storing ginger roots under optimum conditions takes high cost, ginger roots are commonly stored in underground tunnels where the maintenance of optimum temperature and humidity is difficult. One of the methods fur long term storage of ginger roots is freezing. The objective of this research was to evaluate effect of storage temperatures and packaging methods on the quality of minced ginger prepared with frozen stored ginger. The minced ginger prepared with frozen stored ginger at $-20^{\circ}C$ was packed in bags, glass bottles and tubes, and then stored at 5 and $-20^{\circ}C$ for quality evaluation at 4 and 15 week-intervals. The changes of surface color, total free sugars, free amino acids and volatile compounds were less in the combined treatment samples than in control during storage, regardless of the storage temperature. The tube packing was the best for maintaining quality of minced ginger during storage among tested packaging methods. Sensory results showed that the minced ginger with the combined treatment and packed in tubes could be stored at 5 and $-20^{\circ}C$ for 12 and 45 weeks, respectively, without a significant drop in palatability.

Electrowinning of Tungsten From Fused Bath Composed of Calcium Chloride, Calcium Oxide and Tungstic Oxide (텅그스텐의 熔融鹽電解)

  • Kim, Jae-Won;Lee, Dong-Nyung
    • Journal of the Korean Chemical Society
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    • v.10 no.1
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    • pp.32-42
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    • 1966
  • The electrolysis of tungstic oxide dissolved in the bath of calcium chloride and calcium oxide was studied to produce metallic tungsten using carbon as anode and iron as cathode in the temperature range of 900^{\circ}$ to $1200^{\circ}C$. The binary phase diagrams $CaCl_2$-CaO and $CaCl_2-CaWO_4$ systems were constructed to determine the suitability of bath composition and the range of temperatures for the electrolysis. As $WO_3$ reacted with $CaCl_2$ to form oxychloride in the fused salt, the addition of the proper amount of CaO was necessary to avoid the loss of $WO_3$. The optimum compositions of fused bath were $CaCl_2$ 100 parts, CaO and $WO_3$ each 10 to 20 parts, with the CaO, $WO_3$ ratio greater than unity, to keep freezing point low and to prevent the vaporization of $CaCl_2$. The observed decomposition voltage at which $WO_3$ decomposes to W and CO was-0.1 volt, whereas the calculated was -0.3 volt. Metallic tungsten deposited at the cathode reacted easily with CO formed secondarily at the anode surface, to form WC below $1050^{\circ}C$, so that the cell temperature should be above $1050^{\circ}C$. The effects of cathode current densities on current efficiency were minor in the range of 1 to 5 $amp/cm^2$.

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Developing an On-Line Monitoring System for a Forest Hydrological Environment - Development of Hardware - (산림수문환경(山林水文環境) 모니터링을 위(爲)한 원거리(遠距離) 자동관측(自動觀測)시스템의 개발(開發) - 하드웨어를 중심(中心)으로 -)

  • Lee, Heon Ho;Suk, Soo Il
    • Journal of Korean Society of Forest Science
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    • v.89 no.3
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    • pp.405-413
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    • 2000
  • This study was conducted to develop an on-line monitoring system for a forest hydrological environment and its meteorological condition, such as temperature, wind direction and speed, rainfall and water level on V-notch, electrical conductivity(EC), potential of hydrogen(PH) by the motor drive sensor unit and measurement with a single-chip microprocessor as controller. These results are summarized as follows ; 1. The monitoring system consists of a signal process unit, motor drive sensor unit, radio modem unit and power supply. 2. The motor drive sensor unit protects the sensor from swift current or freezing and can constantly maintain fixed water level during measurements. 3. This monitoring system can transfer the data by radio modem. Additionally, this system can monitor hydrological conditions in real time. 4. The hardware was made of several modules with an independent CPU. They can be mounted, removed, repaired and added to. Their function can be changed and expanded. 5. These are the result of an accuracy test, the values of temperature, EC and pH measured within an error range of ${\pm}0.2^{\circ}C$, ${\pm}1{\mu}S$ and ${\pm}0.1pH$ respectively. 6. This monitoring system proved to be able to measure various factors for a forest hydrological environment in various experimental stations.

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