• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.94-102
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• 1984

These experiments were intended to obtain basic information to develop an effective method of seed storage, using local varieties of seeds of 13 different crops in Korea. The germination ratio and velocity were investigated at one month intervals with seeds stored in six different temperatures($-20^{\circ}C$, $-7^{\circ}C$, $7^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$, $50^{\circ}C$) for six months. The results obtained are summarized as follows. 1. Final germination ratio of the seeds of different crops were not influenced by the storage temperatures when dry seeds were used, but moist seeds were affected by storage temperatures. 2. The germination velocity and growth of the primary root of the seeds were remarkably influenced by storage temperatures. Viability of the seeds maintained in freezing temperatures ($-20^{\circ}C$, $-7^{\circ}C$) and in low temperature ($7^{\circ}C$) were higher than that of the seeds stored in high temperature ($30^{\circ}C$).

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.6
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• pp.163-170
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• 2012

With the advantage of a rapid exothermic reaction property, jet set concrete may be used as a cold weather concrete because it can reach the required strength before being damaged by cold weathers. And it can be hardened more quickly if the field temperature is properly compensated by heating. Because ordinary concrete cannot be hardened well under sub-zero temperatures, anti-freeze agents are typically added to prevent the frost damage and to ensure the proper hardening of concrete. While the addition of a large amount of anti-freeze agent is effective to prevent concrete from freezing and accelerates cement hydration resulting in shortening the setting time and enhancing the initial strength, it induces problems in long-term strength growth. Also, it is not economically feasible because most anti-freeze agents are mainly composed of chlorides. Recent studies reported that magnesia-phosphate composites can be hardened very quickly and hydrated even in low temperatures, which can be used as an alternative of cold weather concrete for cold weathers and very cold places. As a preliminary study, to obtain the material properties, mortar specimens with different mixture proportions of magnesia-phosphate composites were manufactured and series of experiments were conducted varying the curing temperature. From the experimental results, an appropriate mixture design for cold weathers and very cold places is suggested.

• Korean Journal of Food Science and Technology
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• v.19 no.2
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• pp.81-88
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• 1987

Rheological properties of rehydrated suspensions of two kinds of freeze dried Kochujang powders, processed at different freezing rates, were compared with raw Kochujang using Brookfield wide-gap rotational viscometer at $25^{\circ}C-60^{\circ}C$. Shear rates ranged from $0.1965\;sec^{-1}$ to $1.9650\;sec^{-1}$ and solid content ranged from 47% to 56%. Rehydrated suspensions of quickly frozen Kochujang powder and slowly frozen Kochujang powder, and raw Kochujang exhibited pseudoplastic behaviors with yield stress and presented thixotropic properties which followed the second-order kinetic behavior proposed by Tiu. Suspensions of Kochujang powders exhibited considerably higher decaying rates than raw Kochujang. The dependency of the equilibrium structure parameter on the shear rate was weak, and there were no significant differences among the values of structure parameters of three samples. The temperature dependency of the apparent viscosity of Kochujang suspension was fully expressed by Arrhenius equation and activation energies of suspensions of quickly frozen Kochujang powder and slowly frozen Kochujang powder, and raw Kochujang were 2.21, 2.18, and 2.32 Kcal/g.mole respectively. Consistency indices of three samples increased with solid content and decreased with temperature. Flow behavior indices of three samples showed no considerable dependency on the temperature and solid content. There were no significant differences in the rheological properties between two Kochujang powders.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.747-751
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• 2010

The objective of this research were to produce amino acids from freezing-dried brown seaweed(Undaria pinnatifida) powder by sub- and supercritical water hydrolysis and to characterize the products. The hydrolysis was carried out in a batch type reactor consisting of 4.6 cm inside diameter and $200cm^3$ vessel and stir made of Hastelloy 276. A stir inside the reactor was continuously moving at 100 rpm. Brown seaweed powder and 100 mL of 1% acetic acid in distilled water were charged into the reactor at a ratio of 1:100(w/v). The applied conditions were $180{\sim}374^{\circ}C$, respectively for 1 hour. The total amino acid content was found to be significantly higher in brown seaweed hydrolyzed by low temperature comparing to high temperature. The amounts of low molecular weight amino acids(glycine, alanine, serine etc) were higher than that of high molecular weight amino acids. The maximum yields of amino acids were produced at low temperature($220^{\circ}C$) with acid catalyst.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.187-192
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• 2022

The repeated monthly or weekly subculture of plant callus is labor intensive and increases the risk of somaclonal variation from the parental callus line. The most effective method for preserving plant callus is cryopreservation, which involves storage in liquid nitrogen. However, this method cannot be applied to the callus of different plant species in the same manner, so it is difficult to develop a standardized cryopreservation method. In addition, the survival rate of the frozen callus after thawing and the regeneration rate after survival are uncertain. Therefore, it is necessary to develop a method to extend the subculture interval of plant callus in an active state. In this study, active plant calli of various species without freezing was incubated at 15℃ for 4 to 12 weeks without subculture. After 12 weeks, 8 lines of plant callus grew less than 2-fold when cultured at 25℃, but at least 2 times as much when cultured at 15℃. Moreover, total antioxidant activity did not differ significantly between plant callus recovered at 25℃ after culturing at 15℃ or at 25℃. These results show that the subculture interval can be extended at a temperature of 15℃ without need for modified medium composition or additional processes. In addition, positive results in all calli of several plant species are expected to reduce labor as well as somaclonal variation by increasing the subculture.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.193-199
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• 2021

Solenopsis japonica, which is belonging to Formicidae in Hymenoptera, is a native ant species in Korea. However, it had not been studied for cold hardiness of S. japonica to understand on its overwintering mechanisms in field so far. Cold tolerance on developmental stages was measured at different cold temperature with various exposure times. Workers showed more survival at 5℃ and 10℃ compared with other stages and elevated cold tolerance when workers were exposed at 15℃ for more than 12h incubation as a rapid cold hardening (RCH) condition. RCH treatment not only increased survival of workers at cold temperatures, but also decreased supercooling point (SCP) and freezing point (FP). RCH group increased the survival rate by 44% at 10℃ compared with Non-RCH group. SCP and FP were depressed from -10.0 to -14.2℃ and from -11.3 to -15.3℃, respectively, after RCH treatment. Cold temperature increased expression level of cold- and stress-related genes such as glycerol kinase and heat shock protein. These results indicate unacclimated cold tolerance of S. japonica and its acclimation to low temperature by RCH.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.486-492
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• 2007

Seminal plasma(SP) is usually removed from semen that is to be cryopreserved. However, some reports indicate that SP has beneficial effects on spermatozoa during chilling and freezing. The purpose of this study was to determine the effect of SP on sperm survival by adding SP to the extender before cooling and freezing canine spermatozoa. In replicate experiments, ejaculates obtained from four healthy dogs(1-4 years old) of various breeds were pooled, centrifuged at $300{\times}g$ for 10 min at $25^{\circ}C$, and the supernatant of seminal plasma was decanted. Spermatozoa were suspended in egg yolk-Tris(EYT) buffer. The study comprised two experiments: [Exp 1] Sperm were suspended in EYT extender containing either 0, 20, 40, 80 or 100% SP and were slowly cooled to $4^{\circ}C$ for 2h or held at $25^{\circ}C$ as controls. Sperm concentration was adjusted to $2{\times}10^8/ml$. [Exp II] Sperm samples, each of which contained $1{\times}10^8/ml$, were assigned to nine groups to be frozen. In the first four groups, sperm in EYT containing either 20, 40, 80 or 100% SP were cooled to $4^{\circ}C$, then diluted to contain final concentrations of EYT+0.6M glycerol and then were frozen. The final concentrations of SP were 10, 20, 40 or 50%. In the other four groups, sperm in EYT alone were first cooled slowly to $4^{\circ}C$, then diluted to contain final concentrations of EYT+0.6M glycerol plus 10, 20, 40 or 50% SP and then were frozen. Spermatozoa, which chilled in EYT alone and diluted to contain final concentrations of EYT+0.6M glycerol without seminal plasma, and then frozen, was regarded as control. Spermatozoa were frozen at $25^{\circ}C/min$ of cooling rate in plastic straws that were suspended above liquid nitrogen and thawed in water at $38^{\circ}C$ for 1 min. Sperm survival was assayed by determining progressive motility and integrity of plasma and acrosome membranes. Progressive motility was determined by microscopic examination at $200{\times}$ magnification. Membrane integrity was assessed by use of a double fluorescent dye, and acrosome integrity by staining sperm with Pisum sativum agglutinin. The results of the first experiment showed that adding SP did not improve motility of spermatozoa compared to those incubated without SP regardless of temperature. The results of the second experiment showed that spermatozoa suspended in EYT+0.6M glycerol containing SP exhibited the higher progressive motility before being frozen(P<0.05). However, frozen-thawed spermatozoa that had suspended in EYT+0.6M glycerol containing SP showed the similar or lower viability(P<0.05). In summary, although seminal plasma did not affect spermatozoa that were chilled in EYT without cryoprotectant(CPA), addition of seminal plasma to EYT containing CPA did significantly improved progressive motility of canine spermatozoa that were chilled.

• Korean Journal of Remote Sensing
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• v.33 no.2
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• pp.243-248
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• 2017

Sea ice which is an important component of the global climate system is being actively detected by satellite because it have been distributed to polar and high-latitude region. and the sea ice detection method using satellite uses reflectance and temperature data. the sea ice detection method of Moderate-Resolution Imaging Spectroradiometer (MODIS), which is a technique utilizing Ice Surface Temperature (IST) have been utilized by many studies. In this study, we propose a simple and effective method of sea ice detection using the dynamic threshold technique with no IST calculation process. In order to specify the dynamic threshold, pixels with freezing point of MODIS IST of 273.0 K or less were extracted. For the extracted pixels, we analyzed the relationship between MODIS IST, MODIS $11{\mu}m$ channel brightness temperature($T_{11{\mu}m}$) and Brightness Temperature Difference ($BTD:T_{11{\mu}m}-T_{12{\mu}m}$). As a result of the analysis, the relationship between the three values showed a linear characteristic and the threshold value was designated by using this. In the case ofsea ice detection, if $T_{11{\mu}m}$ is below the specified threshold value, it is detected as sea ice on clear sky. And in order to estimate the performance of the proposed sea ice detection method, the accuracy was analyzed using MODIS Sea ice extent and then validation accuracy was higher than 99% in Producer Accuracy (PA).

• Journal of the Korean Institute of Landscape Architecture
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• v.40 no.6
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• pp.173-179
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• 2012

The experiments, which analyze the injury symptoms and diagnose growth conditions utilizing IRVT and analyzing each parts of H. helix L., had been held under a low temperature. Greenhouse and outdoor growing Genus hedera had been prepared and compared with each Genus hedera's peak and bottom leaves' surface temperature under the experimental categories $-6^{\circ}C$ and $-12^{\circ}C$. As results, analyzing the surface thermal property of peak part leaves' of outdoor growing Genus hedera, at experimental categories $-6^{\circ}C$, $-12^{\circ}C$ were ranged from $-2^{\circ}C{\sim}-7^{\circ}C$ and $-2^{\circ}C{\sim}-15^{\circ}C$. On the other hand, the surface thermal property of bottom part leaves at experimental categories $-6^{\circ}C$, $-12^{\circ}C$ were ranged $-2^{\circ}C{\sim}-11^{\circ}C$ and $-1^{\circ}C{\sim}-12^{\circ}C$. It appears that the thermal properties of leaves' surface on $-6^{\circ}C$ peaks and $-12^{\circ}C$ bottoms were more broadband than bottoms and peaks. It means that the peaks were more sensitive than bottoms, as like $-2^{\circ}C{\sim}-15^{\circ}C$, $-1{\sim}-12^{\circ}C$. Moreover, as similar results had seen to leaves surface temperature added to cold wind conditions. How the cold wind damaged the outdoor growing Genus hedera, analyzed the surface thermal property by IRVT data under $0^{\circ}C$, $-2^{\circ}C$, $-4^{\circ}C$ condition, it resulted to $-6.2^{\circ}C$, $-6.8^{\circ}C$, $-7.5^{\circ}C$. It appeared more $3.5{\sim}6.2^{\circ}C$ low temperature than experimental setting point. In addition, each parts thurmal property of peaks and bottoms was not similar, it referred to each parts' sensitivities of low temperature were different on the peak and bottom leaves surface temperature.

• Food Science and Preservation
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• v.15 no.5
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• pp.635-641
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• 2008

This study was conducted to investigate the optimal sub-zero temperature for storage of two garlic cultivars, the northern ecotype 'Jaerae' and the southern ecotype 'Daeseo'. These cultivars of garlic bulbs were stored at different temperature (room temperature, $0^{\circ}C$, and $-4^{\circ}C$) conditions after predrying. Southern ecotype garlic showed higher weight loss than northern ecotype garlic. Northern ecotype garlic at $-4^{\circ}C$ exhibited less than 5% of weight loss during 10 month storage. Sprouting rate was higher in southern than in northern ecotype at room temperature storage. The value of injury by disease and insect was similar between southern ecotype and northern ecotype for 5 month storage, and then northern ecotype showed higher value of injury by disease and insect than southern ecotype. Hunter 'b' value of northern ecotype garlic was the lowest at $-4^{\circ}C$ storage. Northern ecotype garlic showed no cold injury at freezing point temperature storage. However, southern ecotype garlic had $0{\sim}5.3%$ cold injury occurrence at $-4^{\circ}C$ storage. Respiration and ethylene production exhibited the highest at room temperature storage, those had the slight increase at $0^{\circ}C$ and $-4^{\circ}C$ storages. Northern ecotype showed higher enzymatic pyruvic acid and fructan contents than those of southern ecotype. Enzymatic pyruvic acid content increased and fructan contents decreased during storage time.