• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.2
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• pp.146-154
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• 2014

In the result of effect on freezing damage reduction by the shade net colors and the shade rate to tea trees during wintering period, the high shade ratio decreased tea trees growth and increased freezing damage and 55% of shade ratio based on non treatment developed new leafs and green leaf productivity. By the shade net colors, colorless shade net (55% of shade type) treatment and green shade net treatment increased green leaf productivity and decreased damaged area compare to non treatment shade net and black shade net treatment. Colorless net shade treatment reduced over 50% of freezing damage and increased (10a) 68 kg for green leaf productivity compare to non treatment shade net. The colors of shade net treatments for reduction freezing damaged in order of Black < Green < Colorless but reduction of freezing damage was so high. In the relative microclimates, treatment shade nets were $0.7^{\circ}C$ lower, average relatively humidity 14.9% higher, soil temperature $0.6^{\circ}C$ lower and soil moisture 4.6% higher than non treatment shade net. And treatment shade nets decreased average wind speed 0.7 m/s and it showed us treatment net shades effected to excessive evapotranspiration and soil dry by wind and considered one of good solution for freezing damages.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.2
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• pp.129-134
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• 1998

The objective of this study was to test whether the developmental capacity of human multi-pronuclear (PN) zygotes after ultra-rapid freezing using EM grid can be maintained. For this experiment, multi-PN zygotes which produced in human IVF program were used as an alternatives of normal 2PN zygotes, and they were separated into 3PN or $\geq4PN$ zygotes to compare their in vitro development and cryoinjury according to PN number. As freezing solution, EFS30 which consisted of 30% ethylene glycol, 18% bcoll, 0.5 M sucrose and 10% FBS added D-PBS was used. The result obtained in this experiment was summarized as follows; When the multi..PN zygotes were ultrarapidly frozen and thawed, the high mean percentages (85.5%) were survived. Also when the cleavage rates between control and freezing group were compared with PN number, there were not significantly different in each group (3PN; 81.3% & 85.4% and $\geq4PN$; 90.0% & 95.7%). When the in vitro development rates after thawing were examined, freezing 3PN group (22.0%) was not differed to control 3PN group (38.5%), although the development result of freezing $\geq4PN$ group (45%) was significantly lower than that of control $\geq4PN$ group (44.4%) (p<0.05). These results demonstrate that developmental capacity of human zygote can be obtained by ultra-rapid freezing method using EM grid and EFS30.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• Journal of Pharmacopuncture
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• v.11 no.2
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• pp.81-85
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• 2008

Objective This study was done to investigate the safety of Bovis Calculus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration (2005. 10. 21, KFDA 2005-60). After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, the auther observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results 1. After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Bovis Calculus pharmacopuncture solutionwas medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions I suggested that Bovis Calculus pharmacopuncture solution didn't induced eye irritation in rabbits.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.17 no.11
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• pp.1088-1094
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• 2005

In the present paper, a new method is proposed for the measurement of the ice packing factor (IPF) of an ice slurry using the index of refraction. The purpose of the new method is to improve the resolution of the measurement and to increase its resistance to electric noise as compared to the standard IPF measurement technique that relies on measurement of the freezing temperature. These two methods are similar in that they both obtain a concentration of aqueous solution from measured physical quantities and calculate the IPF using a relation between concentration and IPF. We experimented and compared the two methods, whose results were also compared with results from the calorimeter method obtaining the IPF directly They are in good agreement (within $5\%$), which demonstrates the validity of the newly proposed method.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.29-29
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• 2003

The objective of this study is to examine an effective cryopreservation method and various vitrification containers on the survival vates of embroys. For the vitrification, in vitro produced embryos at blastocyst stage were exposed to ethylene glycol 5.5 M freezing solution (EG5.5) for 20 sec, loaded on containers such as grid, straw and paper and then immediately plunged into - 196$^{\circ}C$ L$N_2$. The blastocysts were thawed serially in 0.5, 0.25 and 0.125%; P < 0.05). Therefore, this study suggests that bovine embryos can be easily, effectively and successfully cryopreserved by grid, straw, and paper in the presence of freezing solution. Furthermore, vitrification using paper may be used as a no M sucrose in CR1aa, each for 1 min, and cultured in CR1 an medium supplemented with 10% FBS. After thawing, there were not significant differences in recovery rates of EM grid, straw and paper as 84.6, 88.3, and 93.7%, respectively (Table 1). However, survival rates of EM grid (78.1%) and paper (77.1%) showed significantly higher than straw (52. 1w method for bovine embryos.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.1321-1326
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• 2008

A study on the heat and mass transfer for the drying time in spray freezing drying process was experimentally presented in this paper. A spray freezing dryer for the production of an inhalable powder medicine is developed and an albumin solution as protein specimen is adopted. The freeze-drying for the albumin solution is tested in three different height of the particles piled up in a tray such as 2 mm, 5 mm, 7 mm, and the drying time is estimated as 10 h, 15 h, 22 h. From this results, the correlation is suggested and the drying time with various height of the particles piled up in a tray can be estimated. The assessment on the drying time is conducted thought a microbalance and the rate of water content is measured. The results based on temperature and weight are compared. The difference of the results happens due to the heat transfer from the lateral side of the vial. It is thought that the better estimation in drying time can be predicted by considering the heat transfer effect.

• Transactions of the Korean hydrogen and new energy society
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• v.33 no.5
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• pp.541-549
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• 2022

This study investigates anti-icing design by conjugate heat transfer analysis in lab-scale printed circuit heat exchanger (PCHE) for supply of cryogenic high pressure liquid hydrogen. The conjugate heat transfer analysis by using computational dynamics (CFD) provided various temperature distributions at important locations in PCHE heat exchanger and predicted the possibility of freezing in hot channel. And, the effect of inlet temperature of glycol water was analyzed in order to resolve the freezing problem in PCHE.

• Korean Journal of Animal Reproduction
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• v.14 no.1
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• pp.9-16
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• 1990

This study was carried out in order to investigate effects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos. Mouse 2-cell embryos, following dehydration by exposure to DMSO and sucrose, were directly immersed into liquid nitrogen and thawed in 37$^{\circ}C$ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium at various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO concentrations (82.6%). However, when sucrose concentraitons of 0.25 and 0.5 M were added to the freezing medium with 3.0 M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time (2.5min) in 3.0 M DMSO＋0.25 M sucrose (85.9%). 3. The development rate of embryos at in vitro 2-cell, in vitro 2-cell, solution control and untreated control was 84.6%, 90.9%, 89.9%,, and 89.7%, respectively.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.6
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• pp.55-61
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• 2018

This study aims to analyze the performance of Heated-nonslip using renewable energy to prevent nonslip freezing during winter. For this purpose, power generation system and congratulatory devices using solar energy are designed, and it is designed to provide regular electricity to heat up nonslip through Electrical storage system(ESS). In this study, It is intended to analyze the level of electrical energy suitable for nonslip using 24V or 48V, and to measure the temperature changes and temperature distribution according to the location of the test object. As a result of the experiment, nonslip's frame temperature was measured at $-7.5{\sim}-5^{\circ}C$ on average, and $-1{\sim}-2^{\circ}C$ on the heating cable during the supply of 24V and this could not be the solution for defrosting freezing nonslip in the winter. As a result of heating nonslip by supplying 48V with an electrical power of 8W, the temperature of the nonslip was shown to be between $5^{\circ}C$ and $11^{\circ}C$ to $13^{\circ}C$. Even if the power supply was switched on and off every minute, the temperature did not drop below $4^{\circ}C$ and the frozen ice melted on the nonslip without freezing.