• Title/Summary/Keyword: Freeze-dry

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Microwave Vacuum Drying of Germinated Brown Rice as a Potential Raw Material for Enzyme Food (잠재적 효소식품 원료로서 발아현미의 마이크로파 진공건조)

  • Kim, Suk-Shin;Kim, Sang-Yong;Lee, Won-Jong
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1107-1113
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    • 1998
  • This work was to study the potential use of germinated and microwave-vacuum-dried brown rite as a raw material for enzyme food. Brown rice was soaked in water at $15^{\circ}C$ for 2 days and then germinated at $25^{\circ}C$ for 4 days. The germin ated brown rice was then dried by different drying methods: microwave vacuum drying 1(drying only), microwave vacuum drying 2 $(drying{\rightarrow}crushing{\rightarrow}drying)$, hot air drying, vacuum drying and freeze drying. Each drier except freeze drier was set to maintain the sample temperature to be $60^{\circ}C$. During microwave vacuum drying 1 and 2 the sample reached $60^{\circ}C$ much faster (5 min) and was dried much faster (2 to 3 hrs for microwave vacuum drying 1 and 2 than the other drying methods. The initial drying rate of microwave vacuum drying was ten times faster than that of hot air drying. The microwave vacuum drying produced a dry sample of the highly retained enzymic activity, followed by freeze drying, vacuum drying and hot air drying.

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Properties of Cementless Loess Mortar Using Eco-Friendly Hardening Agent (친환경 무기질 고화재를 사용한 무시멘트 황토모르타르의 특성)

  • Jung, Yong-Wook;Kim, Sung-Hyun;Lee, Dong-Gyu
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.3 no.4
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    • pp.355-365
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    • 2015
  • This study examined the fluidity and strength properties, water resistance, durability, and freeze-thaw of cementless loess mortar using an eco-friendly hardening agent. The experimental result indicates that 28 days compressive and flexural strength of the loess mortar was increased regardless of the weathered granite soil and loess mixture ratio as the replacement ratio of the hardening agent increases. The strengths were significantly increased until 14 days regardless of the hardening agent, while the effect on the strengths increasement was relatively low after 14 days. Thus, the strength development of loess mortar concrete was found to be faster than that of the normal concrete. In addition, when the hardening agent of 10% was used, the average flexural strength was 1.7MPa which is insufficient compared to the 28-day flexural strength of 4.5MPa for the paving concrete. However, the flexural strengths of the loess mortar concrete using the hardening agents of 20% and 30% were 4.0MPa and 5.3MPa, respectively. Thus, the hardening agent need to be at least 20% so that the loess mortar can be used for paving concrete. The experiment for water resistance shows that the repeated absorption and dry reduced mass regardless of the mixing ratio of the loess. The maximum length change also decreased with increasing the substitution rate loess mixture ratio and the hardening agent. The result of the freeze-thaw resistance test indicates that the relative dynamic modulus of elasticity at 300 cycle freeze-thaw with the hardening agents of 20% and 30% were 75% and 79%, relatively. Thus, the hardening agent of at least 20% is required to obtain the relative dynamic modulus of elasticity of 60% for the loess mortar.

Effect of feeding garlic leaves on rumen fermentation, methane emission, plasma glucose kinetics, and nitrogen utilization in sheep

  • Panthee, Arvinda;Matsuno, Ayana;Al-Mamun, Mohammad;Sano, Hiroaki
    • Journal of Animal Science and Technology
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    • v.59 no.6
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    • pp.14.1-14.9
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    • 2017
  • Background: Garlic and its constituents are reported to have been effective in reducing methane emission and also influence glucose metabolism in body; however, studies in ruminants using garlic leaves are scarce. Garlic leaves contain similar compounds as garlic bulbs, but are discarded in field after garlic bulb harvest. We speculate that feeding garlic leaves might show similar effect as garlic constituents in sheep and could be potential animal feed supplement. Thus, we examined the effect of freeze dried garlic leaves (FDGL) on rumen fermentation, methane emission, plasma glucose kinetics and nitrogen utilization in sheep. Methods: Six sheep were fed Control diet (mixed hay and concentrate (60:40)) or FDGL diet (Control diet supplemented with FDGL at 2.5 g/kg $BW^{0.75}$ of sheep) using a crossover design. Methane gas emission was measured using open-circuit respiratory chamber. Plasma glucose turnover rate was measured using isotope dilution technique of [$U-^{13}C$]glucose. Rumen fluid, feces and urine were collected to measure rumen fermentation characteristics and nitrogen utilization. Result: No significant difference in rumen fermentation parameters was noticed except for rumen ammonia tended to be higher (0.05 < P < 0.1) in FDGL diet. Methane emission per kg dry matter ingested and methane emission per kg dry matter digested were lower (P < 0.05) in FDGL diet. Plasma glucose concentration was similar between diets and plasma glucose turnover rate tended to be higher in FDGL diet (0.05 < P < 0.1). Nitrogen retention was higher (P < 0.05) and microbial nitrogen supply tended to be higher (0.05 < P < 0.1) in FDGL diet. Conclusion: FDGL diet did not impair rumen fermentation, improved nitrogen retention; while absence of significant results in reduction of methane emission, glucose turnover rate and microbial nitrogen supply, further studies at higher dose would be necessary to conclude the merit of FDGL as supplement in ruminant feedstuff.

Stabilization of Dry Immuno-Diagnostic Kit with Immobilized Antibody and Determination of Its Shelf-Life (항체가 건조상태로 고정된 면역진단키트의 안정화와 Shelf-Life 결정)

  • 이창우;조정환;육순학;권오협;박영남;박세환
    • KSBB Journal
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    • v.13 no.5
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    • pp.502-510
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    • 1998
  • Stabilization of antibody, which is specific to Salmonella typhimurium antigens, present in dry states on membranes was accomplished, and its shelf-life, i.e., duration for maintaining minimum 90% of the initial activity, under optimal conditions was determined. To prepare two major components of an immuno-strip, the antibody was not only immobilized on nitrocellulose membrane surfaces but also placed within the pores of glass fiber membrane after conjugating it with old colloids as signal generator. Among potential stabilizers of the immuno-components, a disaccharide, trehalose, showed a significant protection effect of immunoglobulin structure from thermal energy. Optimal concentrations of trehalose for the respective component were significantly different (8-fold higher for the antibody-gold conjugate than for the immobilized antibody), which probably resulted from distinct densities and configurations of antibody present on the membranes. An additional requirement for the gold conjugate was freeze-drying of this substance such that the conjugate can be readily resolubilized upon contact with aqueous medium. By using the components prepared under optimal conditions, immuno-strips were constructed and exposed to thermal energy. Signals with less than 10% decrease in the intensity were maintained for approximately 21 days at 60$^{\circ}C$. Compared to previous reports, this result represented a 2-year shelf-life at room temperature. it was, however, two times longer if determined from thermal acceleration tests based on the theory of inactivation rate of protein. Such discrepancy between the two estimates could be mainly attributed to errors in accurately controlling temperatures and also to changes in the physical properties of membranes due to a high thermal energy.

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Measurement of Mode I Fracture Toughness of Rocks with Temperature and Moisture Conditions at Low Temperature (저온하에서의 온도 및 함수 조건에 따른 암석의 모드 I 파괴인성 측정)

  • Jung, Yong-Bok;Park, Chan;Synn, Joong-Ho;Lee, Hi-Keun
    • Tunnel and Underground Space
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    • v.11 no.4
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    • pp.352-361
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    • 2001
  • Mode I fracture toughness ( $K_{IC}$) of the frozen rocks and that of the frozen-thawed rocks were obtained by using BDT and CCNBD specimens. The test temperatures ranged from +$25^{\circ}C$ to -16$0^{\circ}C$. Wet and air-dry specimens of granite and sandstone were used in order to investigate the effect of water and porosity on fracture toughness. The SEM images of the frozen-thawed rocks were also analysed to check the density of thermal cracks. The $K_{IC}$ of the frozen rocks increased as the test temperature went down. The rate of increase was higher in wet condition than in dry condition and the rate of increase for wet granite was higher than that for wet sandstone. The $K_{IC}$ of the frozen-thawed rocks varied within 15% from the $K_{IC}$ of the rocks at room temperature. After one freeze-thaw process, thermal crack occurred in granite but no thermal cracks occurred in sandstone. And the crack density was increased as the temperature went down.n.

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Inhibitory Effects of Mulberry Fruit on Intestinal Disaccharidase Activity and Hyperglycemia in Streptozotocin-Induced Diabetic Rats

  • Hong, Jung-Hee;Kim, Sang-Woon;Choi, Kyung-Ho;Choi, Sang-Won;Rhee, Soon-Jae
    • Nutritional Sciences
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    • v.7 no.4
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    • pp.201-207
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    • 2004
  • The current study examined the effects of freeze-dried mulberry fruit on disaccharidase activity in the small intestine and the lowering of blood glucose in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats were randomly assigned to one normal and three streptozotocin (STZ)-induced diabetic groups. The diabetic groups were fed a mulberry fruit-free diet (DM-group), 0.3% mulberry fruit diet (DM-F group) or 0.6% mulberry fruit diet (DM-2F group). After they were fed the experimental diets for three weeks, diabetes was induced with an intraperitoneal injection of streptozotocin 50 mg/kg b.w before sacrificing 9 days later using the same experimental treatments. Analyses of anthocyanins, flavonoid and 1-deoxynojirimycin (DNJ) of lyophilized mulberry fruit were carried out and the major anthocyanins were rutin (142.5 mg), isoquercitrin (10.3 mg), quercetin (5.8 mg), morin (1.6 mg) dihydroquercetin (3.83 mg), cy-3-O-glucopyranoside (230.45 mg) and cy-3-O-rutinoside (131.5 mg) on the basis of 100 g dry weight. Total DNJ content was 2.39 mg/g dry weight of lyophilized mulberry fruit. Blood glucose level decreased in the diabetic mts fed the mulberry fruit supplement. The content of the liver glycogen increased in the diabetic mts fed the mulberry fruit supplement. Disaccharidase activity in the proximal part of the intestine, such as that of maltase, sucrase and lactase in the mulberry fruit supplementation groups, were lower than that of the DM group. These results suggest that mulberry fruit possess a suppressive effect on hyperglycemia, possibly by inhibiting the activity of disaccharidase in the small intestine of rats.

Dyeability of Silk Fabrics Using Extracts of Ligustrum Japonicum Thunb Fruit (광나무 열매 추출물을 이용한 견직물의 염색성)

  • Lee, Hye-Sun;Ko, Sung-Mi
    • Textile Coloration and Finishing
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    • v.22 no.1
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    • pp.71-76
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    • 2010
  • In this study the optimum dyeing conditions and blocking effect of UV deodorization efficiency of Ligustrum japonicum Thunb were investigated. Colorants were water-extracted from Ligustrum japonicum Thunb fruit and freeze-drided to obtain colorants powder. The effects of dye concentration, dyeing temperature, dyeing time, and the number of dipping count were studied. Fastness to dry cleaning, rubbing, perspiration, and light were measured according to KS K 0644, KS K 0650, KS K 0715 and KS K 0700, respectively. In order to examine the dyeability according to dyeing conditions, reflectance of fabrics were measured by using UV/VIS spectrophotometer. The bath ratio was 1:20. Dyeing concentration was 100, 200, 300, 400 and 500% on the weight of fiber. Dyeing time was 20, 40, 60, and 80 minutes. Dyeing temperature was 20, 40, 60, 80, and $100^{\circ}C$. The infrared high pressure dying machine was used. As dyeing concentration increased, dye adsorption increased up to 400% and it slowed down. Dye uptake was increased with raising themperature up to $80^{\circ}C$ and it slowed down. Dye adsorption occurred rapidly at first 20 minutes and then it slowed down and reached almost maximum dye uptake at 60 min. Dye uptake increased by repeated dyeing. Therefore, it is considered that optimum dyeing condition is 400%(o.w.f.), $80^{\circ}C$, 60 min. And repeated dyeing improves dye uptake. Color fastness to dry cleaning and rubbing was good, but light fastness and perspiration fastness was not good. Blocking effect of ultraviolet radiation and deodorization efficiency was good.

Hydrogen Bonding Effect on γ-Ray Irradiated Poly(vinyl alcohol) Hydrogels in Different Drying Conditions

  • Gwon, Hui-Jeong;Jo, Sun Young;Park, Eun Ji;Shin, Young Min;Choi, Jong-Bae;Park, Jong-Seok;Lim, Youn-Mook;Nho, Young-Chang;Kang, Phil Hyun
    • Journal of Radiation Industry
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    • v.6 no.1
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    • pp.89-94
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    • 2012
  • Three-dimensional network hydrogels were prepared by ${\gamma}$-irradiation of aqueous solutions of poly(vinyl alcohol) (PVA) and glycerol (Gly). Oven-drying was used to measure the gel fraction (G), hydration (H) or swelling behavior (S) of the prepared hydrogels. This study made a hypothesis that hydrogen bonds due to addition of glycerol and change of dry states such as freeze-drying (FD), room-drying (RD) and oven-drying (OD) acts on the G, H, and S. Interesting results on the hydrogen bonding effect in the prepared hydrogels are monitored at different drying conditions. The FD samples have a higher G values with increase in glycerol content as compared with the OD and RD samples. The formation of strong hydrogen bonding network among Gly molecules and hydrogel matrix was considered as the main driving force, resulting in the changes in the G, H, and S of the hydrogels under different drying conditions.

Improving the Dyeability of Cotton Fabric with Caesalpinia sappan through Pretreatment with Gelatin (면직물의 젤라틴 전처리에 의한 소목염색의 염색성 향상)

  • Lee, Ji Youn;Jang, Jeong Dae
    • Fashion & Textile Research Journal
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    • v.21 no.4
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    • pp.509-514
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    • 2019
  • Cotton fabrics were treated with gelatin to enhance dyeability and color strength when using Caesalpinia sappan dye. Gelatin was used as the protein and a pad-dry- cure method was used for the treatment process (2, 4, 6, 8, 10, 12, 14g/l concentration). Pretreated fabrics were mordanted with 10% alum. Fabrics were then dyed with freeze-dried sappan wood water extract powder form. Dyed samples were assessed in regards to dyeing behavior and color fastness. Comparing untreated and gelatin treated samples from the SEM images indicated that the Gelatin treatment (10g/l) resulted in an enhanced surface roughness that was relative to that of untreated cotton. Padding cotton with gelatin at 6g/l concentration afforded dyed fabrics with a 2 times increase in the K/S value over that of untreated fabrics. All dyed samples were red color with a significant enhancement in the sample color strength (K/S) being observed for pretreated samples. pH values favor dye absorption with pH 7 yielding the highest color strength. Dyeing at an elevated temperature resulted in a lower color strength and reddish-dull color. Longer dyeing times created greater color strengths for untreated and gelatin treated cotton. Increased dye concentrations resulted in higher K/S values for both gelatin treated and untreated cotton. As for color fastness, gelatin treated and untreated cotton fabrics dyed with sappan wood extract showed a relatively low rating in washing fastness (color change 1 rating), light fastness (1 rating), and rubbing fastness (wet:1-2, dry:3-4 rating).

Prediction of Crude Protein, Extractable Fat, Calcium and Phosphorus Contents of Broiler Chicken Carcasses Using Near-infrared Reflectance Spectroscopy

  • Kadim, I.T.;Mahgoub, O.;Al-Marzooqi, W.;Annamalai, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.1036-1040
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    • 2005
  • Near-infrared reflectance spectroscopic (NIRS) calibrations were developed for accurate and fast prediction of whole broiler chicken carcass composition. The Feed and Forage Foss systems Model 5000 Reflectance Transport Model 5000 with near-infrared reflectance spectroscopy (NIRS)-WinISI II windows software was used for this purpose. One equation was developed for the prediction of each carcass component. One hundred and fifty freeze dried broiler whole carcass samples were ground in a Cyclotech 1,093 sample mill and analyzed for dry matter, protein, fat, calcium and phosphate. Samples were divided into two sets: a calibration set from which equations were derived and a prediction set used to validate these equations. The chemical analysis values (mean${\pm}$SD) were calculated based on dry matter basis as follows: dry matter: 33.41${\pm}$2.78 (range: 26.41-43.47), protein: 54.04${\pm}$6.63 (range: 36.20-76.09), fat 35.44${\pm}$8.34 (range: 7.50-55.03), calcium 2.55${\pm}$0.65 (range: 0.99-4.41), phosphorus 1.38${\pm}$0.26 (range: 0.60-2.28). One hundred and three samples were used to calibrate the equations and prediction values. The software used was modified to obtain partial least square regression statistics, as it is the most suitable for natural products analysis. The coefficients of determination ($R^2$) and the standard errors of prediction were 0.82 and 1.83 for the dry matter, 0.96 and 1.98 for protein, 0.99 and 1.07 for fat, 0.90 and 0.30 for calcium and 0.91 and 0.11 for phosphorus, respectively. The present study indicated that NIRS can be calibrated to predict the whole broiler carcass chemical composition, including minerals in a rapid, accurate, and cost effective manner. It neither requires skilled operators nor generates hazardous waste. These findings may have practical importance to improve instrumental procedures for quick evaluation of broiler carcass composition.