• Journal of Plant Biotechnology
• /
• v.29 no.2
• /
• pp.93-98
• /
• 2002

A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.

• Journal of the Korean Ceramic Society
• /
• v.53 no.1
• /
• pp.116-121
• /
• 2016

The amount of carbon dioxide ($CO_2$) released while producing building materials is substantial and has been targeted as a leading contributor to global climate change. One of the most typical methods of reducing $CO_2$ in building materials is the addition of slag and fly ash, like pozzolan material another method is to reduce $CO_2$ production by developing carbon negative cement. MgO-based cement from the low-temperature calcination of magnesite required less energy and emitted less $CO_2$ than the manufacturing of Portland cements. It is also believed that adding reactive MgO to Portland-pozzolan cements can improve their performance and also increase their capacity to absorb atmospheric $CO_2$. In this study, basic research on magnesia cement using $MgCO_3$ and magnesium silicate ore (serpentine) as the main starting materials, as well as blast furnace slag for the mineral admixture, was carried out for industrial waste material recycling. In order to increase the overall hydration activity, $MgCl_2$ was also added. In the case of the addition of $MgCl_2$as accelerating admixture, there was a promoting effect on the compressive strength. This was found to be due to the production of needle-like dense Mg-Cl hydrates. Mgnesia cement has a high viscosity due to its high specific surface area therefore, when the PC-based dispersing agent was added at a level of more than 1.0%, it had the effect of improving fluidity. In particular, the addition of $MgCl_2$ in magnesia cement using $MgCO_3$and magnesium silicate ore (serpentine) as main starting materials led to a lower expansion ratio and an increase in the freeze-thaw resistance finally, the addition of $MgCl_2$ as accelerating admixture led to good overall durability.

• Geomechanics and Engineering
• /
• v.13 no.1
• /
• pp.25-41
• /
• 2017

In recent years, the amount of sludge ash (SA) has considerably increased due to rapid urbanization and population growth. In addition, its storage in landfills induces environmental pollution and health problems. Therefore, its disposal in an environmentally friendly way has become more important. The main goal of this study is to investigate the reusability of sludge ash as an additive with polypropylene fiber (PF) to stabilize marginal sand based on the compressive strength performances from UCS tests. For this purpose, a series of UCS tests was conducted. Throughout the experimental study, the used inclusion rates were 10, 15, 20 and 30% for sludge ash and 0, 0.5 and 1% for polypropylene fiber by total dry weight of the sand+sludge ash mixture and the prepared samples were cured for 7 and 14 days prior to the testing. Freezing and thawing resistance of the mixture including 10% sludge ash and 0, 0.5 and 1% polypropylene fiber was also examined. On the basis of UCS testing results, it is said that sludge ash inclusion remarkably enhances UCS performance of sand. Moreover, the addition of polypropylene fiber to the admixtures including sand and sludge ash significantly improves their stress-strain characteristics and post-peak strength loss as well as UCS. As a result of this paper, it is suggested that sludge ash be successfully reused with polypropylene fiber for stabilizing sand in soil stabilization applications. It is also believed that the findings of this study will contribute to some environmental concerns such as the disposal problem of sludge ash, recycling, sustainability, environmental pollution, etc. as well as the cost of an engineering project.

• Journal of the Korean Geographical Society
• /
• v.36 no.3
• /
• pp.217-232
• /
• 2001

One of the molt debatable Issues on geomorphological study in Korea should be the discussion over the formation process of gent1e slope surfaces on the piedmont area. In this study, the characteristics of spatial distribution and the formation process of geomorphic surfaces were investigated by classifying the alluvial fans as three geomorphic surfaces alluvial the Bulguksa fault-line The fan surfaces, distributed along the west slue of Bulguksa Mts, consists the confluent alluvial fans continuously along the N-S direction The surfaces of Sincheon-Hyomun district juxtaposed to the Ulsan Bay must be infulenced by sea-level chance during the Quaternary Taken together, these observation suggests that the major four factors contributed to the fan formation 1) rather longer freeze-and-thaw cycle during the Glacial period. 2) the steep mountain slope along the west side of Bulguksa Mts.. which had been resulted from the horizont stress of EAst Sea 3)the tectolinear fault system developed by structural movement along the Bulguksa Fault-line valley. and 4) the erosion-labile characteristics of bedrock In this urea which is consisted of the Bulguksa granite and the sedimentary rock formed in Cretaceous period.

• International Journal of Highway Engineering
• /
• v.12 no.3
• /
• pp.103-111
• /
• 2010

Recently, usage bicycle has been encouraged to reduce energy consumption and $CO_2$. For this purpose, lots of bike road construction are planned. Typical type of pavement used in bikeroad such as asphalt concrete pavement, portland cement concrete pavement, colored pavement, soil pavement. However, these pavement types may need high construction cost comparing the required capacity of bike road. In this study, roller compacted concrete pavement which are economical and durable, are investigated to use as bike road pavement. The optimum compaction level and mix design of roller compacted concrete pavement are suggested by exploring strength test with various mixture ratio and compaction level, Also durability was examined based on freeze-thaw and scaling test. In addition, the cost and amount of carbon emission during in the construction of roller compacted concrete were evaluated and compare with the cost and carbon emission of typical portland cement concrete.

• Tunnel and Underground Space
• /
• v.11 no.4
• /
• pp.352-361
• /
• 2001

Mode I fracture toughness ( $K_{IC}$) of the frozen rocks and that of the frozen-thawed rocks were obtained by using BDT and CCNBD specimens. The test temperatures ranged from +$25^{\circ}C$ to -16$0^{\circ}C$. Wet and air-dry specimens of granite and sandstone were used in order to investigate the effect of water and porosity on fracture toughness. The SEM images of the frozen-thawed rocks were also analysed to check the density of thermal cracks. The $K_{IC}$ of the frozen rocks increased as the test temperature went down. The rate of increase was higher in wet condition than in dry condition and the rate of increase for wet granite was higher than that for wet sandstone. The $K_{IC}$ of the frozen-thawed rocks varied within 15% from the $K_{IC}$ of the rocks at room temperature. After one freeze-thaw process, thermal crack occurred in granite but no thermal cracks occurred in sandstone. And the crack density was increased as the temperature went down.n.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.2
• /
• pp.245-253
• /
• 2014

A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus using submerged culture fermentation. The enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, with a yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDS-PAGE were 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. It hydrolyzed fibrinogen by cleaving the ${\alpha}$ and ${\beta}$ chains of fibrinogen followed by the ${\gamma}$ chains, and also activated plasminogen into plasmin. The enzyme was optimally active at $45^{\circ}C$ and pH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitors of TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partial amino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR, GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree of homology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. The purified enzyme can also be applied as a natural agent for oral fibrinolytic therapy or prevention of thrombosis.

• The Journal of Engineering Geology
• /
• v.13 no.3
• /
• pp.345-358
• /
• 2003

Recently, the reuse of coarse aggregate derived from demolished concrete was introduced into practice with two environmental aspects: protection of natural sources of aggregate and recycling of construction waste. However, recycled aggregate has been used for the very limited application such as subbase material for pavement and constructional filling material because it was considered as low quality constructional materials. In the present study, in order to examine the possibility that recycled aggregate can be used for concrete mixing, we conducted various experimental tests to identify mineralogical, chemical and mechanical properties of recycled aggregate and to determine the workability and mechanical properties of recycled aggregate concrete (RAC). The cement paste and mortar contained in recycled aggregate significantly affect the basic mechanical properties of aggregate and the workability and mechanical properties of RAC. However, RCA mixed with the proper replacement ratio of recycled aggregate shows the comparable compressive strength and freeze and thaw resistance to those of normal concrete. Therefore, it is considered that recycled aggregate can be widely used for concrete if the cement paste and mortar can be efficiently removed from recycled aggregate and/or if the effective replacement ratios of recycled aggregate are applied for mixing concrete.

• Journal of Conservation Science
• /
• v.35 no.4
• /
• pp.279-293
• /
• 2019

The stone lantern of the Damyang Gaeseonsaji temple site is a cultural heritage built during the Unified Silla period (AD 868). The reason for its value as a cultural property is due to wittern the background and the period created on inscription of the lamp stone engraved by letters. The stone lantern consists of two types of lithic tuffs for the 23 original properties, the replaced stones in 1991, and the biotite granite for its ground stones replaced in 2005. The lithic tuffs selected as the replacement parts in 1991 and 2017 have been examined and got to properties of hardly exposure moisture as well as very similar geochemical characteristics. There were various types of physical deterioration of the stone properties and structural cracks; in particular, on the northern side of the stylobates. Chemical and biological deterioration can be identified as black, white, and brown discolorations as well as by the presence of lichens, bryophytes, and herbaceous plants. In the evaluation of the physical properties of the stone lantern, the mean and maximum ultrasonic velocities were found to be similar in each direction. However, the lowest velocity on the east and south sides were found to be lower than those of other stone properties. It was found that physical damage to the stylobates resulted from water expansion in a freeze-to-thaw phenomena related to water content. Therefore, dismantling repair was carried out in the protection facility to restrict further water supply to the stone as much as possible.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.32 no.3
• /
• pp.39-46
• /
• 1990

This paper was analized the thermal conductivity of polystylene (TENSAR- GEOGRID) embeding into the subbase through frost penetration depth, frost heave, change of bearing capacity, and soil moisture movement due to freezing, thawing and icing actions, and their results were as follows : 1.The change of temperature into the sub-base was much increased by the Tensar-Geogrid insertion, and the frost penetration and frost heave were decreased as the thinner of the insulation thickness but the thawing velocity of melting period was appeared to be faster in case of non-insulated. 2.The frost heave had a close relationship with the thickness of insulations which was reasonably included anti-frost effects. 3.The moisture content during the freezing period of upper layer of the insulation insertion was increased by 15 per cent but it was returned to initial state of the thawing period, and at the down layer temporarily increased by 10 per cent and returned to the original state at once. 4.The insulation was acted as a function of distribution of surcharge, and the settlement of the sub-base was about 1.5 mm under 15 tonnage of load and which was included within the allowable limits. 5.The sliding resistance due to the icing which was induced by the insulation insertion into the sub-base was appeared as more 40 per cent than noninsulation area, so that the insulations should be restricted on the place such as mountains, curved and cross area which were required the braking power under the traffics.