• Title/Summary/Keyword: Free fraction

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Heat Inactivation of Proteolytic Enzymes in Papaya Latex (Papaya Latex에 있는 단백질분해효소(蛋白質分解酪素)들의 열불활성(熱不活性)에 관하여)

  • Noh, Bong-Soo;Park, Kwan-Hwa
    • Korean Journal of Food Science and Technology
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    • v.12 no.3
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    • pp.209-215
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    • 1980
  • Proteases from papaya latex were partially purified by ammonium sulfate precipitation and separated into two fractions (Fraction I and II ) by carboxymethyl cellelose column chromatography. Each fraction, mixture of the two fractions, and crude extract of the papaya latex at pH 7.0 were inactivated at the range of $60{\sim}90^{\circ}C$ and thermal properties of the enzymes were investigated. In the thermal inactivation of fraction I, the enthalpy of activation was 89.5 kJ/mol; the entropy of activation, -44.0 J/mol K; the free energy of activation, 104.6 kJ/mol; z-value, $25^{\circ}C$. For fraction II, the enthalpy of activation was 96.5 kJ,/mol; the entropy of activation, -22.0 J/mol K; the free energy of activation, 104.0 kJ/mol; z-value, $23^{\circ}C$. For the mixture of fraction I and II, the enthalpy of activation was 90.9 kJ/mol; the entropy of activation, -38.8 J/mol·K; the free energy of activation, 104.2 kJ/mol; z-value, $24.6^{\circ}C$. For crude extract, the enthalpy of activation was 113.8 kJ/mol; the entropy of activation, 22.0 J/mol·K; the free energy of activation, 106.2 kJ/mol; z-value, $23.2^{\circ}C$. It was indicated that the fraction I was more heat-stable than the fraction II and this suggested that the thermal stability of the proteases in papaya latex is probably due to the fraction I.

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Ramanujan's Continued Fraction, a Generalization and Partitions

  • Srivastava, Bhaskar
    • Kyungpook Mathematical Journal
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    • v.45 no.2
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    • pp.273-280
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    • 2005
  • We generalize a continued fraction of Ramanujan by introducing a free parameter. We give the closed form for the continued fraction. We also consider the finite form giving $n^{th}$ convergent using partition theory.

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Phenolic Composition and Antioxidant Activities of Different Solvent Extracts from Pine Needles in Pinus Species

  • Kang, Yoon-Han;Howard, Luke R.
    • Preventive Nutrition and Food Science
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    • v.15 no.1
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    • pp.36-43
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    • 2010
  • The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.

The antioxidative effects of α-tocopherol on the lipid peroxidation and protein oxidation by free radicals (Free radical에 의한 지질과산화와 단백질산화에 대한 α-tocopherol의 항산화효과)

  • Chung, Chung-won;Huh, Rhin-sou
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.249-258
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    • 1994
  • This study was carried out to investigate the inhibitory effects of vitamin E on the oxidative damage of cellular lipids and proteins in free radical reaction induced by $FeCl_3$, and ascorbic acid. In this experiment, a vitamin E treated rat group was administered with 100mg/kg body weight of $dl-{\alpha}-tocophery$ 1 acetate and an untreated rat group was administered with the same volume of corn oil. And then assays of malondialdehyde and carbonyl group in total homogenate, mitochondrial and microsomal fraction of rat liver were carried out at the scheduled time. The results obtained from this study were summarized as follows; 1. Lipid peroxidation levels in vitamin E administered rat liver cells were significantly (p<0.05) decreased at the intervals between 1 hour and 4 hours in liver homogenate, at all times except for 1 hour point in mitochondrial fraction, and also at the intervals between 0.5 hour and 3 hours in microsomal fraction compared with those of the control rat liver cell. 2. Protein oxidation levels in vitamin E administered rat liver cell were also significantly (p<0.05) decreased at the intervals between 1.5 hours and 4 hours in liver homogenate, at over 4 hours in liver mitochondrial fraction, and at the intervals between 0.5 hour and 3 hours in liver microsomal fraction compared with those of the control rat liver cells.

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A Study on the Change of Free Surface Vortex according to Intake Conditions in the Pump Sump (펌프 섬프장 흡입 조건에 따른 자유표면 보텍스 변동에 관한 연구)

  • Park, Young-Kyu;Li, Kui-Ming;Choi, Yoon-Hwan;Lee, Yeon-Won
    • Journal of the Korean Society of Visualization
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    • v.9 no.4
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    • pp.74-79
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    • 2011
  • In this study the change of free surface vortex is represented at different times according to height of water and with or without curtain wall installation. The air volume fraction is investigated at each condition of water level and the influence about creation of vortex is analyzed. The height of sump intake is taken as 100mm and the water level is divided into 5 steps. The sump model is the TSJ model and the curtain wall is applied by HI standard of America. The results shows that the free surface vortex can be identified on the isotropic surface at air volume fraction 1%~5% and the vortices make an air column from the free surface to the sump intake and are created and destroyed repeatedly. In the higher water level, less air is absorbed into the intake pipe. After curtain wall installation, the suction rate of the air volume fraction is decreased by 6.7%. The result of the vortex motion according to time, works on a cycle.

Visualization of Air Absorption Induced by Free Surface Vortex in the Pump Sump Using Multi-phase Flow Simulation (펌프 섬프장내 자유표면 보텍스에 의한 공기흡입 현상의 가시화)

  • Park, Young-Kyu;Li, Kui. Ming.;Choi, Yoon-Hwan;Lee, Yeon-Won
    • Journal of the Korean Society of Visualization
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    • v.9 no.3
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    • pp.59-64
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    • 2011
  • In this study the change of free surface vortex is expressed through the time volume fraction using multiphase unsteady condition in sump, because in previous studies of the pump sump did not represent the behavior of the free surface vortex exactly due to the reason it was calculated using single phase and steady condition. The reliability of the computational analysis is verified through comparing experimental results with that of present numerical analysis. Homogeneous free surface model is used to apply interactions of air and water. The results show that the free surface vortex can be identified on the isotropic surface at air volume fraction 1%~5%. The vortices make an air column from the free surface to the sump intake and are created and destroyed repeatedly. The behavior of free surface vortex at numerical analysis is quite similar to experimental test. The result of vortex motion according to time, works on a cycle.

Antioxidative Activity of Extrcts from Fruit of Curdrania tricuspidata (꾸지뽕나무 열매 추출물의 항산화 활성)

  • 차재영;조영수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.3
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    • pp.547-551
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    • 2001
  • The comparative activities of acetone, ethanol, and aqueous fractions extracted from fruit powder of Cudrania tricuspidata by different temperature were tested by in vitro experimental models; peroxidation of linoleic acid and autooxidation of rat hepatic and renal microsomes by using thiobarbituric acid (TBA) for assay of free malondialdehyde production, and scavenging activities of free radicals by DPPH (α, α'-diphenyl-β-picrylhydrazyl). In DPPH method, acetone fraction extracted at 30℃ showed the highest free radical scavenging activities and acetone fractions extracted at 30℃ and 60℃ and ethanol fraction extracted at 30℃ showed stronger than BHT (butylated hydroxitoluene) although used ten-fold lower concentrations. In thiocyanate method used linoleic acid an inhibitory effects of all fractions showed higher than control treatment. TBA method used linoleic acid showed the highest antioxidative activity in acetone fraction extracted at 30℃ and 60℃. an inhibition activity against lipid peroxidation in hepatic microsomes of rats showed the highest at acetone faction extracted at acetone fraction among extracted fractions was shown to be the most potent antioxidative properties and this action was more potent in fractions extracted at 30℃ than those extracted at 60℃.

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Free Radical Scavenging Activity of Butanol Fraction from the Fruit of Citrus junos

  • Cho, Eun-Ju;Li, Li;Piao, Xiang-Lan;Lee, Sang-Hyun;Kim, Hyun-Young
    • Preventive Nutrition and Food Science
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    • v.14 no.1
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    • pp.86-89
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    • 2009
  • In this study, we investigated the free radical [1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (${\cdot}OH$) and superoxide anion (${O_2}^-$)] scavenging activity of MeOH extract and 3 fractions of Citrus junos. Of the tested fractions, the BuOH fraction showed the strongest DPPH scavenging activity, showing the $IC_{50}$ values of 63.4 mg/mL. Therefore, we continuously carried out DPPH, ${\cdot}OH$ and ${O_2}^-$ scavenging activity tests of BuOH fraction of Citrus junos. The BuOH fraction of Citrus junos inhibited DPPH radical to 97.5% at a concentration of 1000 mg/mL and the scavenging activities were increased concentration-dependently. In addition, BuOH fraction from Citrus junos also scavenged ${\cdot}OH$ in a concentration dependent manner from 5 to 1000 mg/mL. Furthermore, BuOH fraction showed about 56% ${O_2}^-$-scavengimg activity at 25 mg/mL concentration but, the scavenging activities were not enhanced in a dose dependent manner. The present results suggest that BuOH fraction of Citrus junos would have the protective potential from oxidative stress induced by free radicals.

Study on the Shape of Free Surface Waves by the Scheme of Volume Fraction (Volume Fraction 기법에 의한 자유표면파 형상 연구)

  • Kwag, Seung-Hyun
    • Journal of Advanced Marine Engineering and Technology
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    • v.32 no.8
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    • pp.1215-1220
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    • 2008
  • To obtain the shape of the free surface more accurately, computations are carried out by a finite volume method using unstructured meshes and an interface capturing method. Free-surface flow, which is very important in the fields of ship and marine engineering, is numerically simulated for flows of both water and air. Control volumes are used with an arbitrary number of faces and allows a local mesh refinement. The integration is of second order, with a midpoint rule integration and linear interpolation. The method is fully implicit and uses quadratic interpolation. The solution method of pressure-correction type solves sequentially equations of momentum, continuity, conservation, and two-equations turbulence model. Comparison are quantitatively made between the computation and experiment in order to confirm the solution method.

NUMERICAL DIFFUSION DECREASE OF FREE-SURFACE FLOW ANALYSIS USING SOURCE TERM IN VOLUME FRACTION TRANSPORT EQUATION (볼륨비 이송방정식의 소스항을 이용한 자유수면 유동 해석의 해 확산 감소)

  • Park, Sunho;Rhee, Shin Hyung
    • Journal of computational fluids engineering
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    • v.19 no.1
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    • pp.15-20
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    • 2014
  • Accurate simulation of free-surface wave flows around a ship is very important for better hull-form design. In this paper, a computational fluid dynamics (CFD) code, termed SNUFOAM, which is based on the open source libraries, OpenFOAM, was developed to predict the wave patterns around a ship. Additional anti-diffusion source term for minimizing a numerical diffusion, which was caused by convection differencing scheme, was considered in the volume-fraction transport equation. The influence of the anti-diffusion source term was tested by applying it to free-surface wave flow around the Wigley model ship. In results, the band width of the volume fraction contours between 0.1 to 0.9 at the hull surface was narrowed by considering the anti-diffusion term.