• Animal Bioscience
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• v.35 no.11
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• pp.1771-1786
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• 2022

Objective: Inosine monophosphate (IMP) is a key factor that imparts of meat flavor. Differences in the IMP content in the muscles were evaluated to improve chicken meat quality. Methods: For this study, the IMP content was detected by high performance liquid chromatography. The gene expression profiles of Jingyuan chickens with different feeding patterns and different sexes were analyzed by RNA-sequencing (RNA-seq). Results: Breast muscle IMP content in free-range chickens was extremely significantly higher than that of caged chickens (p<0.01). Breast muscle IMP content in hens was also higher than that of cocks, but the difference was not significant. Correlation analysis showed that the breast muscle IMP content in caged hens and cocks was negatively correlated with the shear force, and the breast muscle IMP content in free-range hens was significantly negatively correlated with the shear force (p<0.05). The two key genes associated with IMP synthesis in chickens with different feeding patterns were glutamate-ammonia ligase (GLUL) and phosphodiesterase 10A (PDE10A). Bioinformatics analysis revealed that the GLUL and PDE10A genes are involved in glutamine biosynthesis and purine salvage pathways respectively. In addition, GLUL expression was positively correlated with the IMP content in caged and free-range chickens, and PDE10A expression was significantly positively correlated with the IMP content in caged and free-range chickens (p<0.05). Conclusion: These findings will facilitate the comprehension of the deposition of IMP in the muscles and thereby aid the process of selection and breeding of good quality local chickens.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.11
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• pp.1536-1541
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• 2000

The present study was carried out to measure changes of feed intake and thirst level caused by water deprivation in goats fed on dry feed and to elucidate the relationship between those two parameters. Water deprivation significantly (p<0.01) decreased cumulative feed intake and rate of eating at 30, 60, 90 and 120 min, respectively, after feed presentation. Cumulative feed intake, after completion of 2 h feeding, was reduced by about 20, 21 and 64 % due to water deprivation during feeding for 2 h (WD2), for 22 h (WD22) and for 46 h (WD46), respectively, compared to free access to water (FAW). Compared to the FAW, WD2, WD22 and WD46 increased thirst level by about 5, 5 and 9 times, respectively. Mean thirst level (X, g/30 min) was negatively correlated with cumulative feed intake (Y, g DM) after completion of 2h feeding (Y=1302-0.2 X, $r^2=0.97$, p<0.05). Water deprivation depressed plasma volume and there was a significant positive regression between plasma volume (X, ml) and cumulative feed intake (Y, g DM) after completion of 2h feeding (Y=-1003+0.6 X, $r^2=0.99$, p<0.01). Mean plasma osmolality (X, mOsmol/l) correlated significantly and negatively with cumulative feed intake (Y, g DM) after completion of 2h feeding (Y=27004-84.9 X, $r^2=0.95$, p<0.05). In conclusion, a decrease of feed intake during water deprivation is mainly due to an increase of thirst level quantitatively, and the act of feeding itself induces thirst more than the length of water-deprivation periods in goats fed on dry feeds. The present findings suggest that plasma osmolality and plasma volume which affect thirst level are involved in the decrease of feed intake in water-deprived goats.

• Food Science of Animal Resources
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• v.21 no.3
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• pp.215-226
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• 2001

The CLA used to add in diet was chemically synthesized by alkaline isomerization method with corn oil. To investigated the effects of conjugated linoleic acid(CLA) added diet feeding on CLA accumulation and blood characteristics of pork, a total of 64 Landrace was fed both CLA-free and CLA-added(0.3, 0.6 and 0.9%) diet for 1∼4 weeks. Cholesterol compositions in blood and CLA contents and fatty acid compositions of loin, belly, bone and skin were determined at 1, 2, 3 and 4 weeks after CLA added at fed. The HDL content in blood of all treatments was higher(P<0.05) than that of control and that of treatment 3 was higher(P<0.05) than that of other treatments among the CLA feeding periods. Palmitic, stearic and linolenic acids composition of loin and belly was increased but oleic, linoleic and arachidonic acids composition of them was decreased according to increasing the CLA feeding periods. CLA contents of loin and belly were higher than that of control and increased according to increasing the quantity of CLA and CLA feeding periods. CLA contents of bone and skin were higher than that of control, too. CLA content of skin was higher than that of bone. It was suggested that CLA could be accumulated in loin, belly, bone and skin by dietary CLA supplementation, and the CLA concentration and fatty acid composition in muscle could be affected by CLA level in diet and feeding period.

• Animal Bioscience
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• v.34 no.2
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• pp.233-242
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• 2021

Objective: The aim of this study was to determine the effect of pineapple cannery by-product (PCB) level on the growth performance and carcass characteristics of finishing Hanwoo steers. Methods: The feeding stage was divided into early and late finishing stages. A total of 60 castrated Hanwoo steers (13.9±0.8 months old, 418.8±36.5 kg initial body weight [BW]) were blocked by initial BW and then randomly allotted into 12 pens (five head/pen). The pens were randomly assigned to control (CONT), low PCB (LPCB), or high PCB (HPCB) treatments. These diets contained 0%, 1.5%, or 3.0% of PCB (on a dry matter [DM] basis; as-fed basis was 0%, 10.6%, or 21.2%), respectively. Results: For the early finishing stage, body weight gain (BWG) and average daily gain (ADG) of the CONT and LPCB feeding groups were greater (p<0.05) than those of the HPCB feeding group. In addition, there were linear and quadratic effects on BWG and ADG with increasing dietary PCB level (p<0.05). The gain to feed (G:F) ratio tends to quadratically decrease with an increasing PCB level in the early finishing stage (p = 0.076). Growth performances of late finishing stage were not affected by PCB level. The marbling score of the LPCB feeding group was similar to that of the CONT feeding group. However, there was a linear decrease (p< 0.05) in marbling score and quality grade among treatments as PCB was increased in the diet. In the longissimus muscle free amino acid profile, histidine composition increased linearly (p<0.05) with an increasing level of PCB. Conclusion: The level of PCB 1.5% DM in diet can be used for finishing steers without any adverse effects on growth and carcass performances. However, there were some negative effects on growth and carcass performance in the HPCB feeding group.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.3
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• pp.393-408
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• 1996

The effect of various levels of ascidian tunic extracts and carophyll pink on the growth rate, pigmentation, lipid and total cholesterol accumulation, and fatty acid compositions were studied in kuruma prawn, Penaeus japonicus. The kuruma prawn was fed the purified diets with or without ascidian tunic extract and carophyll pink at the levels of 100, 200, and 400 ppm for 8 weeks. In the experiment diet with ascidian tunic extracts or carophyll pink, the values of daily growth rate were ranged between $1.065\;to\;1.292%$, compared with control group. The content of astaxanthin in kuruma prawn was not significantly affected by the feeding levels of tunic extracts. Feeding of the tunic extracts, on the other hand, increased the kuruma prawn lipid and total cholesterol content, and pigment deposition in concentration-dependent manners without influencing the free astaxanthin concentration of prawn flesh and heads between two feeding groups(200 and 400 ppm). And it was also demonstrated that the dietary astaxanthin was deposited in kuruma prawn body tissue mainly as astaxanthin esters. The results suggest that the best feeding strategy for pigmentation in kuruma prawns is the diets with ascidian tunic extracts at the level of 4g/kg feed (200 ppm) for 8 weeks.

• Journal of the Korean Dietetic Association
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• v.5 no.1
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• pp.74-84
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• 1999

The purpose of this study was to investigate a plan of middle/high school foodservice systems which could properly provide nutrition for juveniles' health. Questionnaires were developed and distributed to: 245 middle schools with 271 parents, 328 students, 180 teachers, and 345 administrators; 163 high-schools with 223 parents, 466 students, 179 teachers, and 163 administrators in Seoul and Kyunggi province. The results of this study were as followed. For the desirable feeding type as in-school meal plan, 62.2% responded to the current elementary school feeing type:10.3% responded to a lunch-box prepared at home; and 38.0% responded to free dining out type. For a feeding operation type, school administrators, teachers and parents favored the current school feeding systems in elementary schools with proportion of 68.2%, 47.7%, and 87.6% as respectively. Also, 20.3% of school administrators, 22.6% of teachers, and 6.9% of parents preferred contract management. A total of 27.6% of teachers, 9.2% of school administrators, and 3.7% of parents responded to a lunch-box prepared at home. There was a significant difference between the responses for establishing the main body of financial burden to solve the problem of financial burden which could be the most obstacle to bring middle/high school feeding system into operation. For the management of school feeding systems when brought into operation, 88.7% out of 470 responded schools and 89.9% out of 227 responded teachers reported that an expert should manage school feeding systems. For futuristic direction, an effective joint cooking type between schools which may be the way to solve the difficulties in securing the appropriate space and to decrease the financial burden, the problem of transportation for delivering feeding products, low quality of feeding, and sanitation can occur. Therefore, the distance between schools which operate a joint cooking system will affect as a major factor. Furthermore, concrete examination of plans for introduction of various types of school feeding and institutional devices for management system and supervision of operation should become a condition precedent.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.1
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• pp.110-117
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• 1996

To evaluate the feeding effect of oriental medicine on the functional properties of pork, male pigs(Sus scrofa var. domesticus L.) were fed commercial basic diets containing 1.0%, 3.0% and 7.0% of oriental medicine complex from 30 or 45 days before slaughter. The growth pattern and physical conditions of pigs during the feeding period were checked, and after slaughter, the taste of pork and biological characteristics of serum were analyzed. Body weight gain was significantly increased in case of 45 day feeding groups of 3.0 and 7.0% compared with control group (p<0.05), whereas food intakes were slightly decreased in these groups. Triglyceride and total cholesterol levels were effectively decreased in the same feeding groups compared with control group (p<0.01~0.001). Three percent feeding group not only effectively decreased the LDL-cholesterol levels, but also sig-nificantly decreased the atherogenic index in 30 days(p<0.001). Malondialdehyde levels and hy-droxyl radical formations were effectively inhibited in all oriental medicine feeding groups. Superoxide dismutase activities were effectively increased only in 3.0% feeding groups, HDL-cholesterol levels almost did not change in 3.0% and 7.0% feeding groups in 30 days. External and sensory evaluations make satisfactory results in all oriectal medicines feeding groups. According to the experimental results, the growth pattern and physical nomditions of the pigs fed oriental medicine without feeding any antibiotics were relatively superior to those of control group. The authors suggest that, if more than 3.0% of oriental medicine were fed to the pigs from more then 30 days before slaughter the pork is reatively better than those of general pork not only for the modulating the chronic degenerative diseases, but also for its qualities and tastes.

• The Korean Journal of Food And Nutrition
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• v.9 no.2
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• pp.201-212
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• 1996

The purpose of this study was to find an effect of honey on the lipid metabolism of Sprague Dawley rats. All experimental rats were fed ad libitum, for seven weeks, 68% saccharide diet and 10% or 20% honey from acacia, sumac and miscelllaneous flower honey, respectively, and sucrose. The food efficiency ratio of rat taken diet with honey and high fructose of control group was increased in comparison with the control group. The concentration of cholesterol in serum of rats take총 diet with and high fructose of control group was more increased in comparison with the control and normal group. The concentration of H DL-cholesterol in serum of rats taken sumac honey was increased 57.0% in comparison with the control group, but the concentration of VLDL, LDL-cholesterol in serum of rats taken diet 10PA sumac honey was decreased 48.36% in comparison with the control group. The concentration of phospholipid in serum of rats taken diet with 20% acacia or 10% miscellaneous honey was increased 24.7, 16.25%, respectively, in comparison with the control group. The concentration of free fatty acid in serum of rats taken Inlet with sumac or miscellaneous honey and high fructose was increased in comparison with the comparison with the control group. The concentration supplemented diet with acasia honey was increase in comparison with the control group. The concentration of triglyceride in serum of rat was increased by feeding of honey. The concentration of triglyceride in liver was increased, but the level of phospholipid was decreased by feeding of honey.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.233-240
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• 2019

Objective: This study was to investigate the effects of dietary cotton stalk on nitrogen and free gossypol in sheep. Methods: Treatments included 25% cotton stalk (Treat 1), 50% cotton stalk (Treat 2), and a control (no cotton stalk). Six Xinjiang daolang wethers were cannulated at the rumen and duodenum and fed one of these diets. The effects of these diets on nitrogen and free gossypol absorption and metabolism were determined. Fifteen healthy Xinjiang daolang wethers were assessed for daily gain, tissue lesions, and free gossypol accumulation. Results: Dry matter intake decreased with increasing dietary cotton stalk. Total tract dry matter digestibility did not significantly differ among treatments. Dietary cotton stalk significantly decreased volatile fatty acids and increased ammonium nitrogen in the rumen. Nitrogen intake was significantly higher in Treat 2 than in the control or Treat 1. Nitrogen retention and free gossypol intake increased with dietary cotton stalk. Duodenal free gossypol flow did not increase, and free gossypol almost disappeared from the rumen. The free gossypol content of plasma and tissue was increased with dietary cotton stalk with liver free gossypol>muscle free gossypol>kidney free gossypol. Elevated dietary free gossypol decreased platelets, hemoglobin, and serum iron. Aspartate aminotransferase and ${\gamma}$-glutamyltransferase increased in Treat 2. With high long-term dietary cotton stalk intake, liver cells were swollen, and their nuclei dissolved. Renal cells were necrotic and the interstitia were enlarged. Conclusion: With short-term cotton stalk administration, only a small amount of free gossypol is retained in the body. In response to long-term or high free gossypol cotton stalk feeding, however, free gossypol accumulates in, and damages the liver and kidneys.

• Korean Journal of Community Nutrition
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• v.9 no.4
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• pp.528-535
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• 2004

In order to observe the effects of resistant starches on human glycemic response, nine female university students were investigated using cellulose (CED), resistant starch 3 (RS3D) and resistant starch 4 (RS4D) diets. Each woman's blood sugar and insulin, triacylglycerol and free fatty in plasma concentration were measured at fasting state, then 15, 30, 45, 60, 75, 90 and 120 minute after each test diet feeding. Glycemic indices of the Cellulose diet (CED: 57.9 $\pm3.00$), the Resistant starch 3 diet (RS3D: 52.6 $\pm7.9$) and the Resistant starch 4 diet (RS4D: 52.9 $\pm10.2$) were similar to each other, but they were significantly lower in comparison with those of white wheat bread diet (WWBD: 100). Insulinemic indices of the CED (49.8 $\pm8.2$), RS3D (50.0 $\pm7.3$) and RS4D (72.4 $\pm7.7$) were significantly lower in comparison with the white wheat bread diet (WWBD: 100), but among the dietary fiber diets, the insulinemic index of RS4D was significantly higher than the CED and the RS3D. Plasma triacylglycerol contents of the CED, RS3D and RS4D including WWBD showed gradual increase in tendency after lowering in early stage of each test diet feeding, but not significantly different in each dietary fiber added diet. Plasma free fatty acid contents of the CED, RS3D and RS4D including WWBD showed gradual decrease in tendency after each test diet feeding, but not significantly different by each dietary fiber added diet. In above results, we speculate that resistant starch 3 controls rapid elevation of blood sugar by delaying intestinal digestion and absorption of cellulose, but the result appears to be different from RS4 in comparison. Thus, RS3 intakes may contribute to the diet therapy of diabetic humans, but more studies on RS4 is needed in the future. (Korean J Community Nutrition 9(4): 528∼535, 2004)