• 제목/요약/키워드: Free Amino Acids

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야채류(野菜類)의 성분(成分)에 관(關)한 연구(硏究) (Studies on the Components of Vegetables)

  • 조수열;김석환
    • 한국식품영양과학회지
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    • 제5권1호
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    • pp.61-64
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    • 1976
  • P. edulis순(荀)의 free amino acid 및 organic acid의 종류(種類)와 함량(含量)을 측정(測定)한 결과(結果)는 다음과 같다. (1) Free amino acid는 18종(種)이 검출(檢出)되었는데, lysine histidine arginine tryptophan aspartic acid glutamic acid threonine serine proline glycine alanine valine methionine isoleucine leucine tyrosine phenylalanine 등 17종(種)이 정량(定量)되었으며 이들 중 serine이 가장 많았고, 1종(種)은 미확인(未確認)되었다. (2) Organic acid는 11종(種)이 검출(檢出)되었으며 이들 중 formic acid acetic acid oxalic acid maleic acid succinic acid fumaric acid citric acid tartaric acid sorbic acid 등 9종(種)이 확인(確認)되었고, 이들 중 oxalic acid가 가장 많아 291.14mg%이었으며, 2종(種)은 미확인(未確認)되었다.

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재배지역이 다른 어성초의 부위별 화학성분 비교 (Comparison of Chemical Compositions of Houttuynia cordata Thunb Cultivated from Different Local Area)

  • 조영숙;김용택;손미예;최성희;이용수;서권일
    • 한국식품저장유통학회지
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    • 제7권1호
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    • pp.108-112
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    • 2000
  • Proximate composition , volatile compounds, free amino acids, fatty acids and inorganic compounds in leaf, stem and root of Houttyunia cordata Thunb cultivated from two different area, Bosung and Sunchon , were analyzed. Each part of Houttuynia cordata Thunb from both local area showed moisture contents of 80-84% and crude ash contents of 2.1 ∼2.8%. Crude fat and protein contents were less than 3 % showing slightly higher contents in leaf than in both stem and root . Twenty six volatile compunds were identified from the parts of the plant, the volatile contents were high in the oder of leaf, root and stem. Major volatile compounds were mostly derivatives of decanoic acid ; decanoic acid, 20 tridecanoie, decanal and dodecanoic acid. Of free amino acids in leaf asparagine was the highest, while in stem hydroxyproline, proline and arginine were the major free amino acids. Linoleic acid was the highest in the stem and root, and linolenic acid was highest in leaf. the major minerals of all parts were K, Ca. Mg, P, Fe, Zn and Cu, showing highest with K.

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A Study on Salt-fermented Seahorse added with Proteolytic Enzyme (Protamex)

  • LEE, In-Sook;LEE, Min-Ho;JANG, Kyung-Tae
    • 식품보건융합연구
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    • 제6권6호
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    • pp.1-7
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    • 2020
  • We compared the fermentation of 0 to 4 weeks by manufacturing a rapid low salt-fermented seahorse with a commercial Protamex added to the functional food, Hippocampus abdominalis. We studied amino acid composition, content and major amino acids related to flavor during the fermentation process of salt-fermented seahorse. In the enzyme-free group, it showed little change in the content of non-protein nitrogenous compounds, the content of amino acids and degree of hydrolysis. The Protamex enzyme treatment group was rapidly hydrolyzed in one week of ripening, resulting in increased non-protein nitrogenous compounds content, amino acid content and degree of hydrolysis, and minimal changes in the four weeks. The total amino acid contents ratio showed the highest content of glutamic acid in the enzyme additive group, glycine, alanine, which indicates sweet taste, and serine, the content of glycine, alanine, serine, and lysine, indicating sweet taste, has increased significantly over the enzyme-free group. Twenty species of free amino acid in the four-week of salt-fermented seahorse were detected. It detected 43.0% (6 species) in the enzyme-free group and 63.96% (7 species) in the enzyme additive group.

송충이 단백질의 아미노산과 지방산 조성에 대한 연구 (Studies on the Compositions of Amino acid and Fatty acid of Pine caterpillar(Dendrolimus spectabilis))

  • 차월석;신주연김연순
    • KSBB Journal
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    • 제7권3호
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    • pp.186-190
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    • 1992
  • 송충이의 유리 아미노산, 총 아미노산 조성과 총 지방산 조성을 규명하기 위해 Gas chromarography와 HPLC를 사용하여 분리 정량한 결과는 다음과 같다. 조지방 함량은 21.00%이고, 조단백질 함량은 58.47%로 조성되어 있다. 유리 아미노산의 총 함량은 3.65g/100g으로 조성되어 있으며, 유리 아미노산 중에서 필수 아미노산의 총 함량은 1.31g/100g으로 35.89%를 차지하고 있다. 총 아미노산의 총 함량은 41.20g/100g으로 조성되어 있으며 총 아미노산 중에서 필수 아미노산의 총 함량은 14.75g/100g으로 35.80%를 차지하고 있다. 총 지방산 조성은 $C_{18}$=26.81%로 제일 많이 함유되어 있고, $C_{16}$=19.09%, $C_{18:1}$=18.74%, $C_{18:3}$=15.33%, $C_{16:1}$=7.29%, $C_{20}$=5.21%순으로 함유되어 있으며, 불포화 지방산이 45.88%이고, 필수 지방산은 19.73%를 차지하고 있다.

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고려인삼과 장뇌삼의 유리 아미노산 비교 (Differences in Free Amino Acids between Korean Ginsengs and Mountain Ginsengs)

  • 이호재;유병삼;변상요
    • KSBB Journal
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    • 제15권3호
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    • pp.323-328
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    • 2000
  • 한국산 장뇌삼과 재배인삼, 그리고 중국산 장뇌삼으로부터 유 리 아미노산을 추출하여 HPLC를 이용하여 14가지의 유리 아미 노산을 분석하였다. 전체적인 총 유리 아미노산의 크기를 비교 해 보면, P>M>KM4>CMl>KM7>Y>S>K>KM5>J>U>CM2>H> K KM2>KM1>KM6 순이었다 한국산 재배인삼 P의 아미노산 총 함량은 12.46 mg/g으로 가장 높았고, KM6가 6.86 mg/g으로 가 장 낮았다. 유리 아미노산 중에서 Arg은 Y에서 8.77 mg/g 함량 을 보였다. 총 유리 아미노산 중 Arg의 함량 비율은 KM2에서 7 77.78%로 가장 높게 나타났으며, 산지를 고려하지 않고 보면, 장뇌삼이 재배인삼과 구별되는 특정은 없었다. 한국산 장뇌삼은 H His, Met에서 한국산 재배인삼은 Thr이 특이적으로 다른 시료들 에 비해 다량 함유되어 았었고, 중국산 장뇌삼은 Lys만이 높은 함량 수치를 보였다. 특히 Gly은 한국산 시료에서 중국산 시료 에 비해 특이적으로 많이 함유하고 있었고, Lys은 중국산 장뇌 삼에 다른 한국산 시료에 비해 대체적으로 많이 함유되어 았었다

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Effects of Psychrotrophic Bacteria, Serratia liquefaciens and Acinetobacter genomospecies 10 on Yogurt Quality

  • Shin, Yong Kook;Oh, Nam Su;Lee, Hyun Ah;Choi, Jong-Woo;Nam, Myoung Soo
    • 한국축산식품학회지
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    • 제34권4호
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    • pp.543-551
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    • 2014
  • The aim of this study was to evaluate the effect of proteolytic (Serratia liquefaciens, match %: 99.39) or lipolytic (Acinetobacter genomospecies 10, match %: 99.90) psychrotrophic bacteria (bacterial counts, analysis of free fatty acids (FFA) and analysis of free amino acids) on the microbial and chemical properties (yogurt composition), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of yogurt during storage. Yogurts were prepared with raw milk preinoculated with each psychrotrophic bacteria. The total solid, fat, and protein content were not affected by preinoculation, but the pH of yogurt preinoculated with psychrotrophic bacteria was higher than in control. There was a dramatic increase in short chain free fatty acids among FFA in yogurt with Acinetobacter genomospecies 10. For 14 d of cold storage condition, SCFFA was 25.3 mg/kg to 34.4 mg/kg (1.36 times increased), MCFFA was 20.4 mg/kg to 25.7 mg/kg (1.26 times increased), and LCFFA was 240.2 mg/kg to 322.8 mg/kg (1.34 times increased). Serratia liquefaciens (match %: 99.39) in yogurt caused a greater accumulation of free amino acids (FAA), especially bitter peptides such as leucine, valine, arginine, and tyrosine, but SDS-PAGE showed that the inoculation of Serratia liquefaciens did not affect the degree of casein degradation during storage. Taken together, the excessive peptides and FFA in yogurt generated from psychrotrophic bacteria could develop off-flavors that degrade the quality of commercial yogurt products.

한국재래식(韓國在來式) 간장의 맛 성분(成分)에 관한 연구(硏究) (The Taste Components of Ordinary Korean Soy sauce)

  • 김종규;김창식
    • Applied Biological Chemistry
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    • 제23권2호
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    • pp.89-105
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    • 1980
  • Soysauce was made in the salt concentrations of 22.0% and 28.5%, and the changing aspects in nucleotides and their related compounds, free amino acids, free sugars and non-volatile amines which are related to the taste components during the fermentation process with a view to examining the taste components in the ordinary Korean soysauce were studied. And then artificial soysauce was prepared by applying the values derived from the analysis and measurement, and its sensory evaluation was performed. As the result of the sensory evaluation of artificial soysauce prepared according to the value of components analyzed from soysauce which had been fermented for fourty days in the salt concentration of 22.0%, it has been found that artificial soysauce was similar in taste to ordinary Korean soysauce. So, the following facts have been found: glutamic acid and aspartic acid have MSG-like taste, and IMP has a synergistic effect with these acids, which play great roles in ordinary Korean soysauce; both free sugars such as galactose and amino acids such as glycine, alanine and lysine have sweet taste; both amines such as tyramine and histamine, and amino acids such as valine, leucine, isoleucine, and phenylalanine have bitter taste; these components, combined with saline taste of salt and sour taste of organic acids, are assumed to form the unique taste in the ordinary Korean soysauce.

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백미(白米)와 교맥분(蕎麥粉) 혼식(混食)의 영양가개선(營養價改善)에 대(對)한 연구(硏究) (4) - 백미(白米)와 교맥분(蕎麥粉) 혼합사료(混合飼料)의 사육(飼育)한 흰쥐의 Growth Gain과 혈장중(血漿中)의 Branch-Amino Acid Level에 대(對)하여 - (Improving the Nutritional Value of Mixed Diet of Rice and Buckwheat (4) - Growth Gain and the Level of Branch-Amino Acids in Plasma of Rats Fed on Rice Diet Adding Buckwheat -)

  • 이숙희;이현기
    • 한국식품영양과학회지
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    • 제3권1호
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    • pp.7-11
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    • 1974
  • Wistar Strain male rats were divided into four different diet groups and the control group was fed on seven percent casein diet (C), the second with a mixture of 80% of rice and 20% of buckwheat (RW), the third with rice only (R), and the fourth with a mixture of rice and buckwheat of equal nitrogen level (RWS). Each diet group consisted of six rats was fed for three weeks by the adlibitum feeding method. The results of the experiment, i, e, the growth gain and change in the level of branch-amino acids in the plasma determined by the micro-bioassay method were as follows. 1. The group C showed the largest growth gain and the rest did in the order of RW group, R group, and RWS group. 2. It was shown that rats fed on diet of higher protein score tend to have higher level of free branch-amino acids in the plasma. 3. Thus, the protein score may be estimated based on the level of the free branch-amino acids in the plasma.

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정상홍삼과 불량홍삼의 화학적 특성 (Chemical Characteristics of Normal and Inferior Parts in Korean Red Ginseng)

  • 김천석;이종원;도재호
    • Journal of Ginseng Research
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    • 제25권2호
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    • pp.89-93
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    • 2001
  • This study was carried out to investigate a point of difference in chemical characteristics between normal and inferior Korean red ginseng (Naeback red ginseng=red ginseng with white part of clear boundary in phloem and/or xylem of ginseng body, saengnaeback red ginseng=red ginseng with white part of indistinct boundary). content of total sugar as chemical components of naeback and saengnaeback part from Korean red ginseng were less than that of normal part, and content of reducing sugar in normal and saengnaebakc part showed higher than that of naeback part. But differences in content of total phenolic compounds was not found. The content of crude saponin in normal part was highest, amounts of ginsneoside were about same. The content of constituent amino acid in normal part was about 2 times as compared with those in naeback and saengnaeback part in red ginseng. Among the various amino acids, the contents of arginine, glutamic acid, aspartic acid, leucine and alanine of normal and naeback part were higher than others, but in saengnaeback proline, glutamic acid, aspartic acid, threonin and glycine etc. were higher. In the total amount of free amino acids, those in normal, naeback and saengnaeback part were about the same one another, and arginine, glutamic acid and aspartic acid were major free amino acids.

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페파크로마토그라피에 依한 發芽綠豆의 遊離아미노酸代謝의 硏究 (第 1 報${\sim}$第 2 報) (第 1 報) 綠豆發芽에 따르는 遊離아미노酸 及 아미드의 變動에 關하여 (Studies on the Free Amino Acids Metabolism in Germinating Mung Bean by Paper Chromatography. (Part $1{\sim}2$) (Parkt 1). Variation of free Amino acids and Amides contents in germinating Mung Bean)

  • 김태린;송창원
    • 대한화학회지
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    • 제5권1호
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    • pp.9-14
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    • 1961
  • Ethanol extracts of Mung Bean seeds and seedings were analysed by 2-dimensional and circular paper partion chromatography for Nitrogen compounds as a part of the study on the Amino acids metabolism. In the seeds, 18 ninhydrin positive substances were present, before germination, but the number increased to 21 after germination. There were 3 unknown substances and one of it formed newly after germination. After 2-days germination, the amount of amides, such as Asparagine and Glutamine. where increased very large which were very small amount before it. Those were accumulated more in dark place than in light and the amount of Asparigine were more than that of Glutamine. Through the germination, there were large amount of Glutarmic acid, Aspartic acid and Alanine which seems to be concerned in transamination reaction in seedings. Valine, Leucine, and Phenylalanine increased to considerable amount after germination. This is very remarkable fact as those Amino acids were reported to be concerned in transamination reaction recently. ${\gamma}$-amino butyric acid was detected in both Cotyledon and Embroy through the germination. It seemed that there is no any Nitrogen Metabolism in the unbroken seed even if it is preserved very long period.

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