• Journal of information and communication convergence engineering
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• 제5권4호
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• pp.339-345
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• 2007

Video transcoding is a technique used to convert a compressed input video stream with an arbitrary format, size, and bitrate into a different attribute video stream different attributes to provide a efficient video streaming service for the customers is dispersed in the heterogeneous networks. Specifically, frames deletion occur in a transcoding scheme that exploits the adjustment of frame rate, and at this time, the loss in temporal relation among frames due to frame deletion is compensated for the prediction of motion estimation by reusing motion vectors in the would-be deleted frames. But the processing time for transcoding don't have an improvement as much as our expectation because transcoding is done only within the transcoder. So in this paper, we propose a new transcoding algorithm based on prediction period to improve transcoding-related processing time. For this, we also modify the existing encoder so as to adjust dynamically frame rate based on the prediction period and deletion period of frames. To check how the proposed algorithm works nicely, we implement a video streaming system with the new transcoder and encoder to which it is applied. The result of the performance test shows that the streaming system with proposed algorithm improve 60% above in processing time and also PSNR have a good performance while the quality of pictures is preserved.

• 정보관리연구
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• 제41권4호
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• pp.165-186
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• 2010

본 연구는 정보시스템의 운영 효율성과 관리 비용 절감을 도모하고자 불필요한 데이터를 폐기할 수 있는 기준과 근거를 탐색해 보았다. 국내외 법령 및 정책을 문헌조사하여 데이터 폐기 지침에 응용할 수 있는 내용을 도출하였으며, 이를 기초로 데이터 폐기를 위한 기초안을 마련하였다. 공공기관과 지자체 실무자를 대상으로 데이터 폐기 현황에 대한 면담조사와 데이터 폐기 지침 초안에 대한 설문조사를 실시하였고, 관련 이슈사항에 대해 의견을 수렴하였다. 그 결과, 데이터 폐기의 대상 유형과 절차, 폐기 방법 등을 포함한 지침을 마련할 수 있었다. 연구 결과로 마련된 데이터 폐기 지침을 시범 적용해 본 결과, 폐기 대상의 선정, 폐기 절차별 역할 담당자의 선정, 폐기 요청과 심의 등에 누락되거나 보완해야 할 사항은 나타나지 않아 지침이 실효성이 있는 것으로 파악되었다.

• 방송공학회논문지
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• 제22권6호
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• pp.734-743
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• 2017

본 논문에서는 동영상의 압축 정보를 이용하여 동영상 조작 시 발생하는 특징 패턴을 분석하여 동영상의 삭제 여부를 검출하는 기법에 대해 소개한다. 제안 방식에서는 최근 표준 코덱으로 개발되어 향후 널리 사용될 것으로 예상되는 HEVC 코덱을 이용한다. 우선 조작된 동영상과 그렇지 않은 동영상의 HEVC 부호화 패턴 중 분류하기가 용이한 여러 패턴들을 분석하여 특징벡터로 선정하고, 선정된 특징벡터를 기계학습을 통해 학습하여 두 그룹 간의 분류 기준을 모델링하여 동영상에 대한 삭제 여부를 판단한다. 실험 결과, 제안한 방식이 이전의 연구 결과에 비해 HEVC 코덱 환경에서 더욱 효과적으로 삭제 여부를 판단함을 확인하였다.

• 한국공작기계학회논문집
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• 제13권6호
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• pp.87-93
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• 2004

An experimental is carried out to investigate the characteristics of blanking for nickel alloy Alloy42 (t=0.203mm), a kind of IC lead frame material. By varying clearance between die and punch the shapes of shear profile are examined. Finite element analysis with element deletion algorithm for ductile fracture mode is also carried out to study the effect of clearance theoretically and to compare with experimental results. The rectangular shape specimen with four different comer radius is used to study the characteristics of blanking for straight side and comer region simultaneously. As the result the ratios measured k(m experiment of roll over, burnish and fracture zone based on initial blank thickness are compared with those of FE analysis. Both experiment and FE analysis show that the amount of mil over and fracture is increased as the clearance increases. When the radius of comer is less than thickness of blank it has been found that larger clearance is required than that of straight region in order to maintain same quality of shear profile at the comer region.

• 한국정밀공학회지
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• 제18권3호
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• pp.182-188
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• 2001

An experiment is carried out to investigate the characteristics of blanking for copper alloy C194 (t=0.254mm), a kind of IC lead frame material. By varying clearance between die and punch, the shapes of shear profile are examined. Finite element analysis with element deletion algorithm for ductile fracture mode is also carried out to study the effect of clearance theoretically and to compare with experimental results. The rectangular shape specimen with four different corner radius is used to study the characteristics of blanking for straight side and corner region simultaneously. As the result, the ratios measured from the experiment of roll over, burnish, and fracture zone based on intial blank thickness are compared with those of FE analysis. Both experiment and FE analysis show that the amount of roll over and fracture is increased as the clearance increases. It has been found that larger clearance is required than that of straight region when the radius of corner is less than thickness of blank, in order to maintain same quality of shear profile at the corner region.

• 방송공학회논문지
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• 제23권6호
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• pp.886-895
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• 2018

본 논문에서는 동영상의 압축 과정에서 발생하는 규칙성을 이용하여 동영상의 조작 여부를 검출하는 기법에 대해 소개한다. 제안방식은 동영상의 이중 압축과 일부 영상의 조작에 의해 잃어버린 계층적 규칙성을 이용한다. 이러한 불규칙성을 추출하기 위해 HEVC의 기본 단위인 CU와 TU의 분할정보를 이용한다. 성능 향상을 위해 지역적인 정보를 활용하여 CU와 TU의 분할 지도를 제작한 뒤, GoP 단위로 묶어 입력 데이터를 제작한다. 효과적인 분류를 위하여 3차원 합성곱 신경망을 이용하여 동영상의 이중 압축 및 조작 여부를 판단한다. 실험 결과, 기존의 기계학습 알고리즘을 이용한 연구 결과에 비해 더욱 효과적으로 동영상의 조작 여부를 판단함을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제30권5호
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• pp.662-667
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• 2020

Epidermal growth factor receptor (EGFR) mutations are not only genetic markers for diagnosis but also biomarkers of clinical-response against tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC). Among the EGFR mutations, the in-frame deletion mutation in EGFR exon 19 kinase domain (EGFR exon 19-del) is the most frequent mutation, accounting for about 45% of EGFR mutations in NSCLCs. Development of sensitive method for detecting the EGFR mutation is highly required to make a better screening for drug-response in the treatment of NSCLC patients. Here, we developed a fluorometric tandem gene amplification assay for sensitive detection of low-abundance EGFR exon 19-del mutant genomic DNA. The method consists of pre-amplification with PCR, thermal cycling of ligation by Taq ligase, and subsequent rolling circle amplification (RCA). PCR-amplified DNA from genomic DNA samples was used as splint DNA to conjugate both ends of linear padlock DNA, generating circular padlock DNA template for RCA. Long stretches of ssDNA harboring multiple copies of G-quadruplex structure was generated in RCA and detected by thioflavin T (ThT) fluorescence, which is specifically intercalated into the G-quadruplex, emitting strong fluorescence. Sensitivity of tandem gene amplification assay for detection of the EGFR exon 19-del from gDNA was as low as 3.6 pg, and mutant gDNA present in the pooled normal plasma was readily detected as low as 1% fraction. Hence, fluorometric detection of low-abundance EGFR exon 19 deletion mutation using tandem gene amplification may be applicable to clinical diagnosis of NSCLC patients with appropriate TKI treatment.

• Journal of Ginseng Research
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• 제41권2호
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• pp.144-150
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• 2017

Background: The presence of gross deletions in the human immunodeficiency virus nef gene ($g{\Delta}nef$) is associated with long-term nonprogression of infected patients. Here, we investigated how quickly genetic defects in the nef gene are associated with Korean Red Ginseng (KRG) intakein 10 long-term slow progressors. Methods: This study was divided into three phases over a 20-yr period; baseline, KRG intake alone, and KRG plus highly active antiretroviral therapy (ART). nef gene amplicons were obtained using reverse transcription polymerase chain reaction (PCR) and nested PCR from 10 long-term slow progressors (n = 1,396), and nested PCR from 36 control patients (n = 198), and 28 ART patients (n = 157), and these were then sequenced. The proportion of $g{\Delta}nef$, premature stop codons, and not in-frame insertion or deletion of a nucleotide was compared between three phases, control, and ART patients. Results: The proportion of defective nef genes was significantly higher in on-KRG patients (15.6%) than in baseline (5.7%), control (5.6%), on-KRG plus ART phase (7.8%), and on-ART patients (6.6%; p < 0.01). Small in-frame deletions or insertions were significantly more frequent among patients treated with KRG alone compared with controls (p < 0.01). Significantly fewer instances of genetic defects were detected in samples taken during the KRG plus ART phase (7.8%; p < 0.01). The earliest defects detected were $g{\Delta}nef$ and small in-frame deletions after 7 mo and 67 mo of KRG intake, respectively. Conclusion: KRG treatment might induce genetic defects in the nef gene. This report provides new insight into the importance of genetic defects in the pathogenesis of AIDS.

• 한국수산과학회지
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• 제43권6호
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• pp.637-641
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• 2010

The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as $1,024-2,048\;{\mu}g{\cdot}mL^{-1}$. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative ${\beta}$-lactamase gene, and the MIC for ampicillin of the mutant strain was $1{\mu}g{\cdot}mL^{-1}$. In conclusion, our findings suggest that the VPA0477 gene acts as a ${\beta}$-lactamase in ampicillin-resistant V. parahaemolyticus strains.

• Molecules and Cells
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• 제26권3호
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• pp.278-284
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• 2008

An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.