• Journal of information and communication convergence engineering
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• v.5 no.4
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• pp.339-345
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• 2007

Video transcoding is a technique used to convert a compressed input video stream with an arbitrary format, size, and bitrate into a different attribute video stream different attributes to provide a efficient video streaming service for the customers is dispersed in the heterogeneous networks. Specifically, frames deletion occur in a transcoding scheme that exploits the adjustment of frame rate, and at this time, the loss in temporal relation among frames due to frame deletion is compensated for the prediction of motion estimation by reusing motion vectors in the would-be deleted frames. But the processing time for transcoding don't have an improvement as much as our expectation because transcoding is done only within the transcoder. So in this paper, we propose a new transcoding algorithm based on prediction period to improve transcoding-related processing time. For this, we also modify the existing encoder so as to adjust dynamically frame rate based on the prediction period and deletion period of frames. To check how the proposed algorithm works nicely, we implement a video streaming system with the new transcoder and encoder to which it is applied. The result of the performance test shows that the streaming system with proposed algorithm improve 60% above in processing time and also PSNR have a good performance while the quality of pictures is preserved.

• Journal of Information Management
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• v.41 no.4
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• pp.165-186
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• 2010

This study aims to suggest the basis and criterion for the data deletion guideline to make the information systems effective and reduce the cost of system management. To make a frame of the guideline, we researched the laws and policies of USA, UK and Australia and the domestic laws and regulations related with the deletion of records. From this paper research, we prepared the draft guideline and gathered the opinion about it. Through this research and survey, we produced out the guideline including the criteria, the process and the way of data deletion. Adopting the guideline to a sample organization, we couldn't find any problem in deleting the unused data.

• Journal of Broadcast Engineering
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• v.22 no.6
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• pp.734-743
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• 2017

In this paper, we introduce a technique to detect the video forgery using coding pattern analysis. In the proposed method, the recently developed standard HEVC codec, which is expected to be widely used in the future, is used. First, HEVC coding patterns of the forged and the original videos are analyzed to select the discriminative features, and the selected feature vectors are learned through the machine learning technique to model the classification criteria between two groups. Experimental results show that the proposed method is more effective to detect frame deletions for HEVC-coded videos than existing works.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.13 no.6
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• pp.87-93
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• 2004

An experimental is carried out to investigate the characteristics of blanking for nickel alloy Alloy42 (t=0.203mm), a kind of IC lead frame material. By varying clearance between die and punch the shapes of shear profile are examined. Finite element analysis with element deletion algorithm for ductile fracture mode is also carried out to study the effect of clearance theoretically and to compare with experimental results. The rectangular shape specimen with four different comer radius is used to study the characteristics of blanking for straight side and comer region simultaneously. As the result the ratios measured k(m experiment of roll over, burnish and fracture zone based on initial blank thickness are compared with those of FE analysis. Both experiment and FE analysis show that the amount of mil over and fracture is increased as the clearance increases. When the radius of comer is less than thickness of blank it has been found that larger clearance is required than that of straight region in order to maintain same quality of shear profile at the comer region.

• Journal of the Korean Society for Precision Engineering
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• v.18 no.3
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• pp.182-188
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• 2001

An experiment is carried out to investigate the characteristics of blanking for copper alloy C194 (t=0.254mm), a kind of IC lead frame material. By varying clearance between die and punch, the shapes of shear profile are examined. Finite element analysis with element deletion algorithm for ductile fracture mode is also carried out to study the effect of clearance theoretically and to compare with experimental results. The rectangular shape specimen with four different corner radius is used to study the characteristics of blanking for straight side and corner region simultaneously. As the result, the ratios measured from the experiment of roll over, burnish, and fracture zone based on intial blank thickness are compared with those of FE analysis. Both experiment and FE analysis show that the amount of roll over and fracture is increased as the clearance increases. It has been found that larger clearance is required than that of straight region when the radius of corner is less than thickness of blank, in order to maintain same quality of shear profile at the corner region.

• Journal of Broadcast Engineering
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• v.23 no.6
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• pp.886-895
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• 2018

In this paper, we introduce a technique to detect the video forgery by using the regularity that occurs in the video compression process. The proposed method uses the hierarchical regularity lost by the video double compression and the frame deletion. In order to extract such irregularities, the depth information of CU and TU, which are basic units of HEVC, is used. For improving performance, we make a depth map of CU and TU using local information, and then create input data by grouping them in GoP units. We made a decision whether or not the video is double-compressed and forged by using a general three-dimensional convolutional neural network. Experimental results show that it is more effective to detect whether or not the video is forged compared with the results using the existing machine learning algorithm.

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.662-667
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• 2020

Epidermal growth factor receptor (EGFR) mutations are not only genetic markers for diagnosis but also biomarkers of clinical-response against tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC). Among the EGFR mutations, the in-frame deletion mutation in EGFR exon 19 kinase domain (EGFR exon 19-del) is the most frequent mutation, accounting for about 45% of EGFR mutations in NSCLCs. Development of sensitive method for detecting the EGFR mutation is highly required to make a better screening for drug-response in the treatment of NSCLC patients. Here, we developed a fluorometric tandem gene amplification assay for sensitive detection of low-abundance EGFR exon 19-del mutant genomic DNA. The method consists of pre-amplification with PCR, thermal cycling of ligation by Taq ligase, and subsequent rolling circle amplification (RCA). PCR-amplified DNA from genomic DNA samples was used as splint DNA to conjugate both ends of linear padlock DNA, generating circular padlock DNA template for RCA. Long stretches of ssDNA harboring multiple copies of G-quadruplex structure was generated in RCA and detected by thioflavin T (ThT) fluorescence, which is specifically intercalated into the G-quadruplex, emitting strong fluorescence. Sensitivity of tandem gene amplification assay for detection of the EGFR exon 19-del from gDNA was as low as 3.6 pg, and mutant gDNA present in the pooled normal plasma was readily detected as low as 1% fraction. Hence, fluorometric detection of low-abundance EGFR exon 19 deletion mutation using tandem gene amplification may be applicable to clinical diagnosis of NSCLC patients with appropriate TKI treatment.

• Journal of Ginseng Research
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• v.41 no.2
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• pp.144-150
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• 2017

Background: The presence of gross deletions in the human immunodeficiency virus nef gene ($g{\Delta}nef$) is associated with long-term nonprogression of infected patients. Here, we investigated how quickly genetic defects in the nef gene are associated with Korean Red Ginseng (KRG) intakein 10 long-term slow progressors. Methods: This study was divided into three phases over a 20-yr period; baseline, KRG intake alone, and KRG plus highly active antiretroviral therapy (ART). nef gene amplicons were obtained using reverse transcription polymerase chain reaction (PCR) and nested PCR from 10 long-term slow progressors (n = 1,396), and nested PCR from 36 control patients (n = 198), and 28 ART patients (n = 157), and these were then sequenced. The proportion of $g{\Delta}nef$, premature stop codons, and not in-frame insertion or deletion of a nucleotide was compared between three phases, control, and ART patients. Results: The proportion of defective nef genes was significantly higher in on-KRG patients (15.6%) than in baseline (5.7%), control (5.6%), on-KRG plus ART phase (7.8%), and on-ART patients (6.6%; p < 0.01). Small in-frame deletions or insertions were significantly more frequent among patients treated with KRG alone compared with controls (p < 0.01). Significantly fewer instances of genetic defects were detected in samples taken during the KRG plus ART phase (7.8%; p < 0.01). The earliest defects detected were $g{\Delta}nef$ and small in-frame deletions after 7 mo and 67 mo of KRG intake, respectively. Conclusion: KRG treatment might induce genetic defects in the nef gene. This report provides new insight into the importance of genetic defects in the pathogenesis of AIDS.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.6
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• pp.637-641
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• 2010

The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as $1,024-2,048\;{\mu}g{\cdot}mL^{-1}$. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative ${\beta}$-lactamase gene, and the MIC for ampicillin of the mutant strain was $1{\mu}g{\cdot}mL^{-1}$. In conclusion, our findings suggest that the VPA0477 gene acts as a ${\beta}$-lactamase in ampicillin-resistant V. parahaemolyticus strains.

• Molecules and Cells
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• v.26 no.3
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• pp.278-284
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• 2008

An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.