• Asian-Australasian Journal of Animal Sciences
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• v.31 no.3
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• pp.316-326
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• 2018

Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.

• Communications of the Korean Mathematical Society
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• v.30 no.2
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• pp.103-108
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• 2015

Fox [2] presented an interesting identity for $_pF_q$ which is expressed in terms of a finite summation of $_pF_q$'s whose involved numerator and denominator parameters are different from those in the starting one. Moreover Fox [2] found a very interesting and general summation formula for $_3F_2(1/2)$ as a special case of his above-mentioned general identity with the help of Kummer's second summation theorem for $_2F_1(1/2)$. Here, in this paper, we show how two general summation formulas for $$_3F_2\[\array{\hspace{110}{\alpha},{\beta},{\gamma};\\{\alpha}-m,\;\frac{1}{2}({\beta}+{\gamma}+i+1);}\;{\frac{1}{2}}\]$$, m being a nonnegative integer and i any integer, can be easily established by suitably specializing the above-mentioned Fox's general identity with, here, the aid of generalizations of Kummer's second summation theorem for $_2F_1(1/2)$ obtained recently by Rakha and Rathie [7]. Several known results are also seen to be certain special cases of our main identities.

• Korean Journal of Environment and Ecology
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• v.27 no.4
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• pp.417-428
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• 2013

The red fox (Vulpes vulpes), listed as a Class I endangered species by the Ministry of Environment of Korea, has been considered to be extinct in South Korea since the 1980s, and an intensive restoration project has been underway in Sobaeksan national park. This study was carried out to develop a suitable model for the red fox reintroduction program based on Population viability analysis (PVA) by using the VORTEX program. If 10 animals (5 females and 5 males) were continuously released into the initial zero population every year for 10 years, population growth rate and extinction probability over the next 50 years after the introduction of the population were $0.018{\pm}0.204$ and 0.354, respectively; the maximum population size was 116.34 at the 16th year after the first release, and a reduction rate of 1.22 every year from the 17th year was inferred. We found that additional releases would be needed from the 17th year after the initial release to maintain a positive growth rate and to prevent the extinction of the released red foxes, and releasing more than 12 individuals every year would be needed for the long-term, continuous existence of red foxes. By contrast, if fewer than 6 red fox individuals were released the extinction probability over the next 50 years was more than 80%. To maintain the minimum population growth rate, the release of more than 8 individuals were needed for positive population growth. The population growth rate was more stable when 10 animals in the change of their sex rate every year from the set value were released as the female-to- male sex ratio of 6:4 rather than 1:1. However, if the female-biased sex ratio was increased by more than 7:3, a negative population growth was expected. The occurrence rate of roadkill and poaching are important factors in the red fox restoration project. The extinction probability was decreased to 30% if each factor was decreased to 3% based on the standard baseline; however, if each factor was increased to more than 3%, an extinction rate of about 90% was reached over the next 50 years.

• Molecules and Cells
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• v.37 no.3
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• pp.270-274
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• 2014

Lymphatic vessels are essential to regulate interstitial fluid homeostasis and diverse immune responses. A number of crucial factors, such as VEGFC, SOX18, PROX1, FOX2C, and GJC2, have been implicated in differentiation and/or maintenance of lymphatic endothelial cells (LECs). In humans, dysregulation of these genes is known to cause lymphedema, a debilitating condition which adversely impacts the quality of life of affected individuals. However, there are no currently available pharmacological treatments for lymphedema, necessitating identification of additional factors modulating lymphatic development and function which can be targeted for therapy. In this report, we investigate the function of genes associated with Bone Morphogenetic Protein (BMP) signaling in lymphatic development using zebrafish embryos. The knock-down of BMP type II receptors, Bmpr2a and Bmpr2b, and type I receptors, Alk3 and Alk3b, as well as SMAD5, an essential cellular mediator of BMP signaling, led to distinct lymphatic defects in developing zebrafish. Therefore, it appears that each constituent of the BMP signaling pathway may have a unique function during lymphatic development. Taken together, our data demonstrate that BMP signaling is essential for normal lymphatic vessel development in zebrafish.

• Korean Journal of Veterinary Service
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• v.46 no.2
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• pp.137-145
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• 2023

From November 2021 to October 2022, 151 pigs were discarded due to the suspected salmonellosis, when assessed by antemortem inspection at slaughterhouses in Gwangju. Among them, 85 cases shown with the rectal stricture were confirmed to be infected with Salmonella spp, when assessed by postmortem inspection and Salmonella-oriented culture in the laboratory, accounting for 56.3% of the carcass discard cases. Salmonella spp. were isolated in 58 cases (68.2%) out of 85 total discard cases. As for serotyping of the Salmonella isolates, 28 cases (48.3%) belonged to serogroup B and 28 cases (48.3%) belonged to serogroup C, those were the most frequent isolates, and 2 cases (3.4%) belonged to serogroup E. The most prevalent serotype was Rissen (39.7%), followed by I4,[5],12:i:-(37.9%), Derby (8.6%), Bareilly (5.2%), Infantis (3.4%), Give (3.4%), and Typhimurium (1.7%). The 58 isolates of salmonellae showed resistance to tetracycline (63.8%), chloramphenicol (56.9%), ampicillin (53.4%), and ampicillin-sulbactam (51.7%). S. Rissen showed resistance to chloramphenicol (82.6%), ampicillin (52.2%), ampicillin-sulbactam (52.2%), and tetracyclin (47.8%). S. I4,[5],12:i:-was resistant to tetracycline (90.9%), ampicillin (81.8%), ampicillin-sulbactam (77.3%), and chloramphenicol (59.1%). Among the isoates, S. Bareilly (3 strains), S. Infantis (2 strains), S. Give (2 strains), S. Rissen (3 strains), and S. I4,[5],12:i:- (1 strain) showed no resistance to the tested antimicrobials. As for the prevalence of the antibiotic resistance pattern, AM-SAM-TE-C was the most common, which was shown in 15.5% of S. Rissen. At second, the resistance pattern was occurred as AM-SAM-TE (12.1%) and AM-SAM-CF-CZ-FOX-CTX-NA-TE-C (12.1%), respectively in the case of S. I4,[5],12:i:-.

• Computational Structural Engineering
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• v.2 no.3
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• pp.69-75
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• 1989

The primary purpose of using bracings is to improve the lateral rigidity of main structural system, i.e., columns and beams, by reinforciing them with much smaller members. In conventional design methods brackings are considered as tension-only members, since difficulties arise in the analysis when the P-.DELTA. effects and post-buckling behaviour of the bracing members are taken into account. This is particulary true fox X-bracings. Recently, however, both analytical and experimental studies have been conducted to investigate the more precise and real behaviour of bracing members, especially for the nonlinear and plastic behaviour under cyclic loads. In this study, an analytical model is proposed to investigate the nonlinear behavior of steel bracing members subjected to cyclic loads. Results of the analysis were compared with previous experimental results, and good agreements were obtained between these results.

• Journal of KSNVE
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• v.4 no.4
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• pp.479-486
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• 1994

The Finite Element Method(FEM) generally used for the structural analysis has some defects, i.e. a great deal of computational time and huge memory capacity of computer are needed in the application to large and/or complex structures, etc. Therefore the Component Mode Synthesis method(CMS), one of sub-structure synthesis methods, was made to improve such demerits and has been developed up to now. In optimum structural modification problems, the sensitivity analysis method is useful, where the sensitivity-calculated by Fox's suggestion-is defined as the diffentials of design variables for the objective values. This paper discusses the vibration minimization techniques for the oper box type structure, in which it is assumed that an engine operates at 10-40Hz range. The results obtained are as follow; (1) The sensitivity of natural frequency could be easily obtained by sensitivity analysis method and the optimum position to insert pillars could be found by using it. (2) The rates of structural modification could be exactly obtained and the natural frequency observed could be easily shifted to the objective value. (3) The maximum amplitude around natural frequency noted could be nearly reduced to 1/25 by modification.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.5
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• pp.481-491
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• 2018

Allergic asthma is one of the most enduring diseases of the airway. The T-helper cells and regulatory T-cells are critically involved in inflammatory responses, mucus hypersecretion, airway remodelling and in airway hyper-responsiveness. Cigarette smoke (CS) has been found to aggravate inflammatory responses in asthma. Though currently employed drugs are effective, associated side effects demand identification and development of novel drugs with negligible or no adverse effects. Rutin, plant-derived flavonoid has been found to possess antioxidant and anti-inflammatory effects. We investigated the ability of rutin to modulate T-cells and inhibit inflammation in experimentally-induced asthma in cigarette smoke exposed mice. Separate groups of neonatal mice were exposed to CS for 10 days from post-natal days 2 to 11. After 2 weeks, the mice were sensitized and challenged with ovalbumin (OVA). Treatment group were given rutin (37.5 or 75 mg/kg body weight) during OVA sensitization and challenge. Rutin treatment was found to significantly inhibit cellular infiltration in the airways and Th2 and Th17 cytokine levels as well. Flow cytometry revealed effectively raised $CD4^+CD25^+Fox3^+$ Treg cells and supressed Th17 cell population on rutin treatment. Airway hyper-responsiveness observed following CS and OVA challenge were inhibited by rutin. $NF-{\kappa}B$ and iNOS, chief regulators of inflammatory responses robustly activated by CS and OVA were down-regulated by rutin. Rutin also inhibited the expression of matrix metalloproteinase 9, thereby aiding in prevention of airway remodelling in asthma thereby revealing to be a potent candidate in asthma therapy.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.13 no.1
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• pp.157-184
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• 2000

Bromidrosis is a disease which is also called huchou[호격] in the oriental medicine. This disease produces an odor and a small amount of sweat from the axillary area. Prognosis of the disease could possibly get better with age. This can be explained by the fact that its symptoms begin usually when a person reaches puberty and as the person gets old, the symptoms reduce or disappear even when specific treatments are not applied. Although the disease is not lethal, many patients have difficulties in leading a normal and comfortable life, physically and psychologically with this in mind and some osbservations gained from the literature studies on the causes,symptoms, and treatments, I embarked on a study aimed at finding out the therapies. My study on the literature has produced the following results ; 1. In the oriental medicine, hu-chou[호격] means the characteric smell or odor produced from the fox. 2. The literature studies point out two causes in the oriental medicine. One is natural. The other is acquired. Acquired cases have the following causes: shi-re-nei-yun[濕熱內蘊]. xie-뱌-bu-huo[血氣不和], gan-qi-yu-jie[肝氣鬱結]. 3 There are two therapies in the oriental medicine. One is internal and the other external. out of the two, external therapies were mainly used according to the literature studies that I have consulted. 4. According to my study, the most frequently used herbal medicine was Alunitum(明 礬)(45 times), followed by Minium(密陀僧)(39 times) and Moschus(?香)(21 times respectively). 5. My study also found that bian-zheng[辨證] was not .rarely listed on old literatures. Therefore, old literatures did not clearly show how to root out the disease. They only taught now to alleviate the symptoms and remove the characteric odor.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1589-1595
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• 2014

Objective: Forkhead box C2 (FOXC2) is a member of the winged helix/forkhead box (Fox) family of transcription factors. It has been suggested to regulate tumor vasculature, growth, invasion and metastasis, although it has not been studied in cervical cancer. Here, we analyzed FOXC2 expression in cervical tissues corresponding to different stages of cervical cancer development and examined its correlation with clinicopathological characteristics. In addition, we examined the effects of targeting FOXC2 on the biological behavior of human cervical cancer cells. Methods: The expression of FOXC2 in normal human cervix, CIN I-III and cervical cancer was examined by immunohistochemistry and compared among the three groups and between cervical cancers with different pathological subtypes. Endogenous expression of FOXC2 was transiently knocked down in human Hela and SiHa cervical cells by siRNA, and cell viability and migration were examined by scratch and CCK8 assays, respectively. Results: In normal cervical tissue the frequency of positive staining was 25% (10/40 cases), with a staining intensity (PI) of $0.297{\pm}0.520$, in CIN was 65% (26/40cases), with a PI of $3.00{\pm}3.29$, and in cancer was 91.8% (68/74 cases), with a PI of $5.568 {\pm}3.449$. The frequency was 100% in adenocarcinoma (5/5 cases) and 91.3% in SCCs (63/69 cases). The FOXC2 positive expression rate was 88.5% in patients with cervical SCC stage I and 100% in stage II, showing significant differences compared with normal cervix and CIN. With age, pathologic differentiation degree and tumor size, FOXC2 expression showed no significant variation. On transient transfection of Hela and SiHa cells, FOXC2-siRNA inhibition rates were 76.2% and 75.7%; CCK8 results showed reduced proliferation and relative migration (in Hela cells from $64.5{\pm}3.16$ to $49.5{\pm}9.24$ and in SiHa cells from $60.1{\pm}3.05$ to $44.3{\pm}3.98$) (P < 0.05). Conclusion: FOXC2 gene expression increases with malignancy, especially with blood vessel hyperplasia and invasion degree. Targeted silencing was associated with reduced cell proliferation as well as invasion potential.