• Title/Summary/Keyword: Forensic

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Comparative study of serological detection methods on old bloodstain samples (오래된 혈흔 시료에 대한 혈청학적 식별법의 비교 연구)

  • Lee, Minji;Ahn, Eu-Ree;Kim, Da-Hye;Shin, Heejin;Jung, Ju Yeon;Lee, Sung-Jin;Chun, Byung-Won
    • Analytical Science and Technology
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    • v.31 no.5
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    • pp.201-207
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    • 2018
  • Blood is a commonly found body fluid at crime scenes, and plays an important role in identifying suspects and in the reconstruction of crime scenes. Although serological detection of blood has been widely used in the field of forensic science, research on the detection of old bloodstains is scarce. This work aimed to compare various methods for the detection of old bloodstains and validate the reliability of their results. Four presumptive tests-Tetramethylbenzidine, $Bluestar^{(R)}$, Leucomalachite Green, Kastle-Meyer tests-and two confirmatory tests-Fecal Occult Blood (FOB) and Rapid Stain $Identification^{(TM)}-Blood$ ($RSID^{TM}-Blood$) tests-were compared. Bloodstain samples from post-mortem cases were collected on gauzes and then stored at room temperature for periods from 7 to 30 years. All the presumptive tests were positive, even for the 30-year-old sample. However, FOB and $RSID^{TM}-Blood$ provided false negative results for some samples stored for 17 years or more (1988 to 2001). The results indicate that FOB and $RSID^{TM}-Blood$ are not reliable for the detection of old bloodstains. These findings can be useful in the selection of an appropriate detection method for serological testing of old bloodstains. In addition, the information will be useful background knowledge when applied in the field of forensic practice.

Identification of Food-Poisoning Bacteria (Bacillus cereus) and the Bacterial Toxin Genes for Application to Forensic Microbiology : A Case Report from National Forensic Service (법미생물 검사를 위한 식중독 세균(Bacillus cereus)의 동정 및 독소 유전자 검사법: 국립과학수사연구원 사례보고)

  • Cho, Yoonjung;Lee, Min Ho;Kim, Hyo Sook;Eom, Kiyoon;Kim, Min-Hee;Kim, Jong-Bae;Lee, Dong Sub
    • Journal of Science Criminal Investigation
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    • v.11 no.3
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    • pp.210-217
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    • 2017
  • In the forensic microbiology laboratories, microorganism analyses from food are requested. There have been several cases of Bacillus cereus isolated from the samples requested to the National Forensic Service. B. cereus is an important pathogenic bacterium which can cause food-borne outbreaks. Therefore, we isolated B. cereus from anchovy aekjeot recently requested for microbial examination and identified using MSId based on the 16S rDNA sequence and real-time PCR method. We also conducted PCR for detection of diarrheal toxin genes and an emetic toxin gene and found the presence of nheABC, bceT and entFM diarrheal toxin genes in the B. cereus isolate. There are several clinically important food-poisoning bacteria that should be noted during inspection. In particular, B. cereus can cause food poisoning even when cooked foods are ingested, because B. cereus forms endo-spore which confers strong environmental resistance and heat resistance to the bacteria, and the bacterial emetic toxin also has heat resistance. Here we highlight the importance to distinguish clinically important bacteria such as B. cereus from food specimens, and we expect this study will provide procedures for identification of B. cereus and detection of the bacterial toxin genes for future cases in the forensic microbiology laboratories.

A Survey of Radiologic Science Students' Awareness and Educational Needs of Forensic Medicine (방사선학과 전공 학생들의 법의학에 대한 인식과 교육 요구도 조사)

  • Kyeong-Hwan Jeong;Sang-Hyun Han
    • Journal of the Korean Society of Radiology
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    • v.17 no.6
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    • pp.977-983
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    • 2023
  • Due to the development of the domestic economy and science, the people's standard of living has increased. Accordingly, we want to improve the quality of life. In other words, we guarantee human rights and pursue dignity and value as human beings. Therefore, the medical field extends human life and helps maintain a healthy life. The social medicine that protects human rights is forensic medicine. Forensic medicine identifies deaths and analyzes the cause using forensic radiology images. Forensic radiology is the acquisition and provision of medical images by the radiographer. Therefore, the radiographer must have expertise by completing forensic science-related courses. Recently, medical and nursing schools have opened and operated various subjects such as forensic medicine and forensic nursing. However, the Department of Radiology science is the only school that offers courses related to forensic science. For the future development and exploration of the radiographer and department of radiology science, forensic education should be considered. For this purpose, we investigated the necessity and demand for forensic education in the department of radiology science undergraduate and graduate schools. The department of radiology science students' awareness of forensic science was found to be 2.977 points, but the need for forensic science education for the radiographer was high at 3.759 points. In addition, current students' demand for forensic science courses was high at 84.1%, with the majority responding that it was necessary to open and operate the course. This study was able to determine the demand for forensic science-related subjects among the department of radiology science undergraduate and graduate students, and there is a need to explore diversity and expertise in education. We hope that it will be used as basic data for the development of forensic medicine and radiology science.

On the Availability of Anti-Forensic Tools for Android Smartphones (안드로이드 스마트폰을 위한 앤티-포렌식 도구들의 활용성)

  • Moon, Phil-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.6
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    • pp.855-861
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    • 2013
  • Smartphone is very useful for use in the real life through the improvement of computing power, faster data rate and the variety of applications. On the other hand, using the smartphone has been exposed to a lot of crime. Also, it occurs attempting to delete a data of smartphone memory by anti-forensic tools. In this paper, we investigate and analyze the anti-forensic tools used in the Android smartphone to study the characteristics and techniques of anti-forensic tools. In addition, experiments are performed to validate the availability of anti-forensic tools by the Oxygen Forensic Suite that is a commercial forensic tool.

Practical and Legal Challenges of Cloud Investigations (클라우드 환경에서 수사 실무와 법적 과제)

  • James, Joshua I.;Jang, Yunsik
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.14 no.6
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    • pp.33-39
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    • 2014
  • An area presenting new opportunities for both legitimate business, as well as criminal organizations, is Cloud computing. This work gives a strong background in current digital forensic science, as well as a basic understanding of the goal of Law Enforcement when conducting digital forensic investigations. These concepts are then applied to digital forensic investigation of cloud environments in both theory and practice, and supplemented with current literature on the subject. Finally, legal challenges with digital forensic investigations in cloud environments are discussed.

Unexpected Death by Sepsis of Staphylococcus aureus with Infective Endocarditis and Paravertebral Abscess in a Fisherman during Sailing out for Fish: An Autopsy Case (심내막염과 척추 주위 농양을 동반한 황색포도알균에 의한 출어 중의 선상 패혈증 사망: 증례 보고)

  • Kim, Youn Shin;Hwang, In Kwan;Moon, Seohyun;Park, Ji Hye;Lee, Young Seok
    • The Korean Journal of Legal Medicine
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    • v.42 no.4
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    • pp.153-158
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    • 2018
  • Staphylococcus aureus is an important cause of human infections, and it is also a commensal that colonizes the nose, axillae, vagina, throat, or skin surfaces. S. aureus has increasingly been recognized as a cause of severe invasive illness, and individuals colonized with this pathogen are subsequently at increased risk of its infections. S. aureus infection is a major cause of skin, soft tissue, respiratory, bone, joint, and endovascular disorders, and staphylococcal bacteremia may cause abscess, endocarditis, pneumonia, metastatic infection, foreign body infection, or sepsis. The authors describe a case of a fisherman who died of sepsis on a fishing boat during sailing out for fish. The autopsy shows paravertebral abscess, pus in the pericardial sac, infective endocarditis with vegetation on the aortic valve cusp, myocarditis, pneumonia and nephritis with bacterial colonization, and also liver cirrhosis and multiple gastric ulcerations.

A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • Biomedical Science Letters
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    • v.28 no.4
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    • pp.327-333
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    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

Effect of novel luminol-based blood detection reagents on DNA stability (새로운 루미놀 기반 혈흔 탐지 시약이 디엔에이에 미치는 영향에 대한 연구)

  • Jung, Ju Yeon;Oh, Yu-Li;Lee, Jee Won;Lim, Seung;Kim, Jung-mok;Lee, Yang Han;Lim, Si-Keun
    • Analytical Science and Technology
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    • v.31 no.2
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    • pp.71-77
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    • 2018
  • Detection of bloodstains is a very important process in scientific investigations, and luminol is often used for the detection of bloodstains that are not visible. Recently, new preparation methods of blood detection reagents based on luminol (BloodFlareA, B) were developed and reported to have higher active persistence and to be more economical than conventional blood detection reagent, BlueStar forensic. In this paper, we tested the specificity and effect of the BloodFlares (A and B) on DNA and compared them with those of BlueStar forensic. False positive results for the BloodFlares were not observed in semen, saliva, vaginal fluids, urine, sweat, and nasal discharge, but were observed in $CuSO_4$, $FeSO_4$, and bleach solutions, and the observed patterns were similar to those of BlueStar forensic. The effect on DNA was determined by analyzing the DNA yield, degradation index, and DNA profiling. Based on these results, we concluded that the BloodFlares based on luminol do not affect DNA stability and are applicable in forensics.

Analysis on Mobile Forensic of Smishing Hacking Attack (Smishing 사고에 대한 Mobile Forensic 분석)

  • Park, Dea-Woo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.12
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    • pp.2878-2884
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    • 2014
  • The Smishing attacks are caused using smartphone since 2013. Smishing hacking attacks are increasing due to the approximately 104 million private information leakage incidents by the 3 domestic credit card companies occurred in January 2014. The Smishing attack occurred in conjunction with hacking illegal leakage of personal information and direct financial damage. In this paper, i am analyze real-world case studies in the lab and study accident on Smishing Mobile Forensic analysis. I am study of a real case Smishing hacking attacks. And studying evidence for a Mobile Forensic analysis of the technical principles of Smishing attacks. The study for the Mobile Forensic evidence proved the Smishing hacking attacks using Mobile Forensic technic and create Mobile Forensic reports. Through this paper, the research will be safe for the people living in the smartphone can be used safely and conveniently, with the development of Mobile Forensic technology, to study the extraction of Smishing accident evidences from the court.

Method validation of detecting ethanol metabolites (EtG, EtS) in post-mortem spleen (비장 조직에서 에탄올 대사체(EtG, EtS)를 검출하는 방법과 유효성 확인)

  • Kim, Soo-Min;Jo, Young-Hoon;An, Song-Hie;Lee, Woo-Jae;Kwon, Mia
    • Analytical Science and Technology
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    • v.34 no.3
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    • pp.115-121
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    • 2021
  • Ethyl glucuronide (EtG) and ethyl sulfate (EtS), which are ethanol metabolites, are direct indicators of ethanol intake; they have been studied in a variety of biological samples in forensic science. It is necessary to analyze ethanol metabolites to determine whether the ethanol detected in autopsy cases was due to alcohol consumption before death or due to the ethanol produced from post-mortem decay. In general, EtG and EtS are detected in the blood together with ethanol; however, it may be difficult to secure blood depending on the extent of decay. Therefore, the aforementioned method should be replaced by detecting the ethanol metabolites using tissue biological samples. In this study, we determined the optimal experimental conditions for detecting EtG and EtS from spleen samples using Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS). Herein, the test method was validated, and an analysis method was applied to the actual autopsy cases.