• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.69-74
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• 2008

This study aims to establish the efficient soybean transformation system and develop soybean [Glycine max (L.) Merill] transformants using cotyledonary node explants. The cotyledonary node of soybean were co-cultivated with Agrobacterium tumefaciens strains (KYRT1, EHA105). These strains contain the binary vector pCAMBIA3301 which carries a herbicide-resistant far gene. Korean cultivars (Danbaekkong, Eunhakong) and foreign cultivars (Jack, Peking) were the most efficient in regenerating cotyledonary node. Therefore, they were chosen for the transformation. Results showed that the T-DNA transfer reached up to 60% and transformation efficiency reached up to 3% in the cotyledonary node explants from Jack cultivar, co-cultivated with EHA105 strain. Histochemical GUS evaluation showed that 12 individual lines, transformed with the 현 gene, have positive response. The transformed soybeans have been confirmed in the $T_0$ generation through phenotypic assay using herbicide $Basta^{(R)}$ and Southern blot analysis.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.235-240
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• 2002

We report the new method for the screening of genetically modified potato by competitive duplex-PCR using the potato specific single oligomer primer for the internal control and CaMV 35S promoter or NOS terminator specific primers. The single oligomer primer (rAGU4A) amplify the potato specific internal control band from the homozygous potato genomic DNA in the RAPD profiles of all analyzed potato varieties. The 530 bp internal control DNA was amplified independently to CaMV 35S promoter or NOS terminator DNA and identified as repetitive or microsatellite DNA of potato (AF541972). With this new technique, the transgenic potatoes which were transformed with vectors contained the different foreign genes are analyzed. In case of the commercialized transgenic potato varieties, 'Hew Leafs', those two genetic factors are used for promoter and terminator respectively So, this new PCR technique should be a promising method of cost effective and accurate screening for the commercialized GM potatoes on market.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.4
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• pp.297-302
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• 2015

This experiment was conducted to evaluate the agronomic characteristics and forage production of Italian ryegrass cultivars at three locations (Gurae, Sungju and Cheonan) from 2013 to 2014. The experiment employed a randomized complete block design with three replications. The seven cultivars used in this experiment were 'Korwinearly', 'Florida80', 'YounbongI', 'YounbongII', 'YounbongIII', 'KeimyungI' and 'KeimyungII' varieties. Italian ryegrass varieties were seeded on October 4 at Gurae, October 8 at Seongju and October 3 at Cheonan in 2013. The Italian ryegrass varieties were harvested on April 26 at Gurae and Seongju, and on April 24 at Cheonan in 2014. Although the seven cultivars had resistance to foliar diseases and insects and high winter survival, they has little lodging resistance. The heading dates for 'Younbong I', 'Younbong II' and 'Keimyung I' were earlier than for other varieties at the three locations. Dry matter (DM) content and plant height in the early maturing cultivars were higher than in other cultivars at the three locations. The fresh and DM yields of the early maturing cultivars were also higher than others at the three locations. The results of this experiment indicate that the heading date for early maturing cultivars is earlier, and the plant height is higher than for other cultivars. Early maturing cultivars had higher DM content as well as fresh and DM yields of Italian ryegrass. Therefore, early maturing cultivars should be recommended as high-yield forage in double-cropping systems because of their superior production of forage and early heading date.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.39-44
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• 2013

In Korea, wide spread use of whole cottonseed, which is primarily a GMO plant imported from foreign countries and being fed to animals as raw state, has aroused concern that it may disturb the existing ecology of the country unless dispersion of the seed is under proper control. The objective of this study was to elucidate the changes in various nutritive parameters due to heat treatment and to determine the effective condition for removing germination ability of whole cottonseed (WCS). Of the various temperatures applied (76, 78, 80, 85, $100^{\circ}C$/30 min) $85^{\circ}C$ for 30 min was confirmed to be the lowest temperature treatment which resulted in a complete removal of the germination ability of WCS. Therefore, based on the determined temperature condition ($85^{\circ}C$ 30 min) we tried to examine the changes of various nutritional parameters, including nutrient composition, in vitro digestibilities and ruminal protein degradabilities, comparing raw whole cotton seed (RWCS) and heated whole cotton seed (HWCS). Some changes in amino acid composition were observed with heat treatment of WCS, but these were regarded to originate from the variation in plant quality and seed morphology, which are usually affected by different environmental factors during the vegetation period. As for fatty acid composition, no significant differences were observed to occur during heat treatment. However, WCS heated at $85^{\circ}C$ for 30 min in a circulating oven showed a significant decrease (p<0.05) of in situ rumen degradability in both dry matter (DM) and crude protein (CP), as compared to raw WCS. Overall results obtained in the study indicate that the heating condition used in this study, which was proven to be the most appropriate and economic to remove germination ability of WCS, may also improve the nutritional value of the ruminant with regard to reducing its protein degradability within the rumen.

• Journal of Plant Biotechnology
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• v.40 no.3
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• pp.135-140
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• 2013

A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.

• Journal of Mushroom
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• v.17 no.4
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• pp.171-178
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• 2019

Agaricus bisporus is a functional food and among the most widely cultivated mushrooms in the world. In this study, we analyzed the genetic diversity and population structure of 23 Korean and 42 foreign A. bisporus cultivars using SSR (Simple sequence repeat) markers. Genetic diversity of A. bisporus cultivars was as follows: number of alleles was approximately 13; observed and expected heterozygosity were approximately 0.59 and 0.74, respectively; and polymorphic information content value was about 0.71. A. bisporus cultivars were divided into three groups using distance-based analysis. Genetic diversity of Group 2, which consisted of cultivars from various countries, was high. In addition, model-based subpopulations were divided into two, and the genetic diversity of Pop2 (Population 2), which had many cultivars, was high. The results of this study could be used in a breeding program for A. bisporus, such as the development of new genetic resources and securing diversity.

• Ecology and Resilient Infrastructure
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• v.2 no.1
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• pp.80-92
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• 2015

Some of alien plants, which were introduced from foreign countries, have caused problems in Korea. Invasion of these alien plants in the ecosystem threatens the habitat of endemic species, reducing biodiversity, and causing a disturbance in the ecological system. In urbanized areas of campus universities, a diverse range of organisms were found and a comparison between the sites, near roads or housing sites were made because the campuses provided a large biotope. Although the campus had been exposed to interferences like gardening, it was also a place for most organisms to live in an active floating population due to free access. This research investigated the flora of alien plants that appeared in Beakseok University, Sangmyung University, Hoseo University and Dankook University, and relationship between the distribution of alien plants and the campus and green areas and distance from the highway. The total number of plant species and naturalized species found in the four universities was 189 and 43 species. Those of Dankook University were 136 and 35 species, Hoseo 108 and 25 species, Sangmyung 103 and 31 species, and Baekseok 97 and 26 species, respectively. The abundance of natural plants for each respective university tended to be higher as it became closer to the highway. Also, the closer the walking distance to the university, the greater the tendency for the degree of similarity to be higher. As a result, we may conclude that the distribution of alien plants and anthropogenic activities may be closely related.

• Journal of Plant Biology
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• v.2 no.1
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• pp.14-21
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• 1959

The bamboo, one of staples of South-East Asia, has 1, 250 species in 46 Genus throughout the world South-East Asia, it's main habitat, has 1, 180 species, while South America has about 70 species or so. Major places of it's production in Asia are Korea, southern China, Japan, Malay, Ceylon, Formosa, and Philipine, but of these, Korea, southern China, and Japan are the most famous places for their production of excellant bamboo. In Korea, Kyongsang-Namdo and Cholla-Namdo are known as it's home, occupying 80 per cent of the total area of bamboo production in this country. Kyongsang-Pukdo, Cholla-Pukdo, Chungehong-Namdo, and Kangwon-do are also known as it's minor production. The importance of the bamboo can not be ignored, considering it's role in the industrial development of this country; the bamboo-ware is indispensable for the culture of laver which annually secures a considerable amount of foreign money. But it has long been regreted that the home-made bamboo-ware is far from being able to meet the ever increasing demand in quantity and is forced to be imported annually. In the light of the above mentioned fact, much interested; I undertook the task of investigating the bamboo in this country, Primarily concerned in it's kind and distribution, and could draw the following conclusions as the first phase of my investigation: (1). The amount of the bamboo accumulation in this country, compared with that of pre-worldwar II, is reduced as much as 300, 000 fagots(on fagot amounts to 25 pieces), and now the acreage of the bamboo field is 32, 200$\textrm{km}^2$s. (2). At a mountian peak in Kunoe-myon Wando-kun Cholla-Namdo, I found out a new specis. (Shasamorpha chiisanensis NAKAI Forma wan-Ponia H. P. CHONG) (3). The kinds of the autogenous wild bamboo in this country are as follows; a). 8 kinds in Sinoarundinaria. (5 Spp; 2 For; 1 Var) b). 1 kinds in Pieioplastus. (1 Spp) c). 2 kinds in Pseudosasa. (1 Spp; 1Var.) d) 4 kinds in Sasamorpha. (2 Spp; 1 For.; 1 Var.) e). 4 kinds in Sasa. (4 Spp) In total there are nineteen(19) kinds of autogenous wild bamboo in this country. When adding to this thethirty five (35) species which the japanese has transplanted in Kyongsang-Namdo Forestry Expreimental stations, there are in all fifty four (54) spe ies in this country. (4). As for the distribution of the Northern limitation of the bamboo growing, I found that Sinoarundinaria nigra OHWI var, Henonis HONDA can be cultivated as for as Kosongkun, Kangwon-Do, andSinoarundinaria pubescens HONDA as for as Clung Chong Namdo, and Sinoarundinaria reticulata as far as chung Chong-Pukdo.

• Korean Journal of Construction Engineering and Management
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• v.9 no.4
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• pp.173-181
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• 2008

Last decade, Korea's export scale has resulted in remarkably increasing tendency, and the Korean construction firms(KCFs)' activities on the global markets also have been revitalized. Therefore, this paper analyze a correlation between changes in the oversee market conditions and firms' competitiveness focused on plant business. The aim of this paper is to analyze the internal competency change of the KCFs on the global markets in the past 5 years and to find gap of internal competencies between KCFs and outstanding foreign firms. From a survey analysis this paper found that the external impacts on the global markets(changes of exchange rates, raw materials prices, supply-demand conditions, etc.) have highly influenced the sales amount of the KCFs. But the impacts to change the operating profit have been analyzed as not important. So it is necessary to reinforce the KCF's internal competencies rater than expecting an improvement of the external conditions. Also, the KCFs should strengthen the design engineering as a core competency.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.253-261
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• 2007

Molecular and cultivation techniques were used to characterize the bacterial communities of biobead reactor biofilms in a sewage treatment plant to which an Aerated Up-Flow Biobead process was applied. With this biobead process, the monthly average values of various chemical parameters in the effluent were generally kept under the regulation limits of the effluent quality of the sewage treatment plant during the operation period. Most probable number (MPN) analysis revealed that the population of denitrifying bacteria was abundant in the biobead #1 reactor, denitrifying and nitrifying bacteria coexisted in the biobead #2 reactor, and nitrifying bacteria prevailed over denitrifying bacteria in the biobead #3 reactor. The results of the MPN test suggested that the biobead #2 reactor was a transition zone leading to acclimated nitrifying biofilms in the biobead #3 reactor. Phylogenetic analysis of 16S rDNA sequences cloned from biofilms showed that the biobead #1 reactor, which received a high organic loading rate, had much diverse microorganisms, whereas the biobead #2 and #3 reactors were dominated by the members of Proteobacteria. DGGE analysis with the ammonia monooxygenase (amoA) gene supported the observation from the MPN test that the biofilms of September were fully developed and specialized for nitrification in the biobead reactor #3. All of the DNA sequences of the amoA DGGE bands were very similar to the sequence of the amoA gene of Nitrosomonas species, the presence of which is typical in the biological aerated filters. The results of this study showed that organic and inorganic nutrients were efficiently removed by both denitrifying microbial populations in the anaerobic tank and heterotrophic and nitrifying bacterial biofilms well-formed in the three functional biobead reactors in the Aerated Up-Flow Biobead process.