Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Transgene structures of marker-free transgenic Bt rice plants

무선발 형질전환 Bt벼의 도입유전자 구조 분석

  • Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Seung Bum (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lim, Myung-Ho (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Gwon, Sun-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Jin-Hyoung (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Cho, Hyun-Suk (National Academy of Agricultural Science, Rural Development Administration (RDA))
  • 우희종 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 이승범 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 임명호 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 권순종 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 이진형 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 신공식 (경기도 수원시 농촌진흥청 국립농업과학원) ;
  • 조현석 (경기도 수원시 농촌진흥청 국립농업과학원)
  • Received : 2013.04.01
  • Accepted : 2013.06.26
  • Published : 2013.09.30
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Abstract

A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.

마커프리 형질전환 식물체 개발 방법의 하나인 무선발 형질전환은 선발마커 유전자를 이용한 도입유전자의 선발과정 없이, 연쇄중합반응(PCR)과 같은 직접 분석방법을 사용해 도입유전자가 도입된 형질전환 식물체를 개발하는 방법이다. 현재까지 감자, 담배, 알팔파, 밀, 땅콩 등 여러 작물에서 무선발 형질전환을 이용한 마커프리 형질전환체가 보고되었다. 그러나 대부분 무선발 형질전환체의 분석이 $T_0/T_1$세대에서의 PCR을 이용한 도입유전자 확인과 후대검정으로 수행되어, 유전자의 안정적 도입 확인을 위한 분자생물학적 특성평가 결과는 보고된 바 없다. 본 연구에서는 4계통의 무선발 형질전환 Bt벼에 대한 도입유전자 특성을 분자생물학적 방법으로 검정하여 유전자도입 위치, 도입유전자의 구조, 벡터 삽입여부 등의 분석 결과를 제시하였다.

Keywords

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