• 한국작물학회지
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• 제55권2호
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• pp.139-143
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• 2010

콩의 생산성을 높이는데 중요한 역할을 하는 효소의 활성변화와 종실 수량과의 관련성을 탐색하기 위해 등숙관련효소인 ADP-glucose pyrophosphorylase(AGP)의 활성변화를 콩 품종별로 등숙기간에 따라 조사한 결과를 요약하면 다음과 같다. 1. 풍산나물콩은 협수 및 잎수가 131개 및 102개로 가장 많았으며, 100립중은 10.4g으로 가장 낮았으나 수량은 275kg/10a으로 가장 높아 물질생산 및 건물축적 효율이 우수한 것으로 나타났는데, 개화시기$(R_1,\;R_2)$에 AGP의 활성도 가장 높은 경향이었다. 2. 품종별 $CO_2$ 동화량은 풍산나물콩이 $20.96{\mu}mol\;m^{-2}s^{-1}$로 가장 많았으며 검정콩1호는 $12.54{\mu}mol\;m^{-2}s^{-1}$로 가장 적었다. 3. 단파흑은 개체당 잎면적이 $3,968cm^2$로 가장 많고 100립중도 30.5g으로 가장 높은 반면 수량은 149kg/10a으로 가장 낮아 건물축적 효율이 가장 낮았으며, 생육단계별 AGP활성도 가장 낮은 수치를 나타냈다. 4. AGP의 small subunit은 60KD의 single band를 나타냈는데 개화기 이후 AGP의 활성변화와 일치하는 경향을 보였다.

• 한국초지조사료학회지
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• 제17권2호
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• pp.167-176
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• 1997

A field experiment was conducted in Chungiu and Jungwon to evaluate growth characteristics, root development, dry matter yield, crude protein yield and palatability according to nitrogen fertilizer level at intercropping cultivation of sorghum $\times$ sudangrass hybrid(hereinafier referred as SSH) and soybean. Nitrogen level was six treatment of Okg, 30kg 60kg, 90kg 120kg, and 150kg per hectare, and cutting date (sorghum $\times$ sudangrass and soybean) was july 28. 1. In the SSH, plant length was increased with increasing nitrogen fertilizer from 0kg/ha to 120kg/ha, but 150kgha treatment was decreased cornpared to 120kg/ha treatment. In the soybean, plant length did not show difference among 0kg, 30kg 60kg, and 90kg per hectare, but 120kg and 150kgfha treatment was on the contrary short. In the SSH, leaf length, leaf width and leaf number were the highest in 120kg/ha treatment, but soybean showed multifarious. 2. Stem diameter of SSH was increased with increasing nitrogen fertilizer. But soybean was thick from 0kg/ha treatment to 60kg/ha treatment, while above 90kg/ha treatment was on the contrary thin. Stem of SSH was hard with increasing nitrogen fertilizer, but soybean was soft with increasing nitrogen fertilizer. Root weight (Dry matter) of SSH was increased with increasing nitrogen fertilizer. On the contrary, soybean was decreased with increasing nitrogen fertilizer. 3. In the dairy cattle and Korean native cattle, palatability according to nitrogen fertilizer was the highest at 90kgha treatment, while 120kgha treatment and 150kgha treatment was the lowest. But in the deer, 30kg/ha treatment of low nitrogen fertilizer was the highest in palatability. 4. Nitrogen content of root of SSH was increased with increasing nitrogen fertilizer(P<0.01). But in soybean, 30kg and 60kg/ha treatment were higher than 0kg, 90kg, 120kg and 150kg/ha treatment(P<0.01). Total niwogen content of soil were high at 90kg 120kg 150kgha trea~ment(P<0.01). 5. Dry matter yield was high at the 90kg 120kg and 150kgha treatment as range from 15, 262kg/ha to 15, 614kg/ha without significant difference among those, but Okgha treatment was the lowest as 11, 183kg/ha (PC 0.05). Protein content of SSH was the highest at 90kg/ha treatment as 8.3 percentage. Soybean was highest at 60kgha treatment as 22.9 percentage. Protein yield was the highest at 90kg/ha treatment as 1, 547.6kg/ha, but Okgha treatment was the lowest as 1, 022.8kg/ha (P<0.01).

• 한국초지조사료학회지
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• 제16권2호
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• pp.105-112
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• 1996

In order to select the forage cowpea of high dry matter and protein yield, growth characteristics and yield performance of forage cowpea were investigated. The results obtained are as follows: Arn0r.g the five varieties tested, lT820-889 and lT83S-852 were top grass type, but IT830422 and lT84E-124 were short grass type. In stem diameter of soilage cowpea, IT83S-852 was the highest as 9.5mrn, but Swwon was the lowest as 7.8mm. In stem hardiness, lT84E-124 was the highest as 1.9kg/$cm^2$, but Seowon was the lowest as 0.8kg/$cm^2$. The palatability was high in the order of lT83S-852 > IT820-889 > Swwon, while IT820489 and IT 83S- 852 were lower than other varieties in 1990 and 1991 year. But palatability of cowpea was wholly lower than other soilage(Sudangrass hybrid and Soybean). In total dry matter yield and protein yield, lT820-889 and IT83S-852 were higher but IT830422 and lT84E- 124 were lower than other varieties. In conclusion, among the five varieties used, IT820-889 and lT83S-852 were higher as forage cowpea.

• 한국초지조사료학회지
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• 제17권2호
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• pp.177-186
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• 1997

A field experiment was conducted to evaluate growth characteristics, dry matter yield and crude protein yield according to different planting dates at sorghum $\times$ sudangrass hybrid(SSH) and soybean intercropping. Planting dates were five treatment of may 6(Tl), may 13(T2), may 20(T3), may 27(T4) and june 3(T5), and cutting frequency was two times a year. 1. Plant length of SSH was the highest at T2 as 253cm, but T5 was the shortest as 203cm. In the soybean, T3 and T4 were the highest as 113cm, respectively. Leaf length of SSH was high at T5. In the soybean, T2 was the highest as 17cm. Average leaf width of T2, T3 and T4 was higher than TI and T5. 2. Leaf number of T3(SSH and soybean) was higher than other treatments, Stem diameter of SSH and soybean showed the highest as 12.3mm and 8.6mrn at T5 and T3, respectively. In the SSY mean stem hardness of TI was the highest as 2.5kg/$cm^2$, but soybean was the highest at T1(8.0kg/$cm^2$) 3. Deed stubble according to move seeding date of SSH were 11.4 percentage at TI, and 3.9 percentage at T5 treatment. 4. Total dry matter yield according to move seeding date was the highest at T3 as 20,937kghq but T5 of late seeding was the lowest as 16,04Okgha(P < 0.05). 5. In the first cutting time, protein content of SSH was the highest at T3 as 9.9 percentage, but T1 was the lowest as 8.4 percentage. In the 2nd cutting, T5 was the highest as 8.7% but T1 was the lowest as 6.2%. In the soybean, T5 was the highest as 19.4% but TI of early seeding was the lowest as 16.2 percentage. Crude protein yield was the highest at T3 as 2,233.5kghq but TI of early seeding was the lowest as 1,579.7kgha (P < 0.05). As mentioned above the results, T2(may 13), T3(may 20) and T4(may 27) treatment could be recommended as the best suitable seeding date when drymatter and protein yield were considered.

• Asian-Australasian Journal of Animal Sciences
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• 제19권5호
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• pp.699-704
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• 2006

Twenty-four cannulated Small-tailed Han${\times}$Poll Dorset wethers (BW $47.5{\pm}2.1kg$) were used to determine the effects of forage to concentrate ratio (40:60 vs. 70:30), monensin supplementation (0, 15 or 30 ppm, DM basis) and interactions of these two factors on cis-9, trans-11 conjugated linoleic acid (cis-9, trans-11 CLA) and trans-11 octadecenoic acid (trans11-$C_{18:1}$) concentrations in ruminal contents and plasma in sheep. The experiment was designed as a $2{\times}3$ factorial. The diet contained Chinese wild rye grass hay (Aneurolepidium Chinese), cracked corn, soybean meal, NaCl, limestone and trace mineral premix. Dietary crude fat and linoleic acid ($C_{18:2n-6}$) were adjusted with soybean oil to about 7.0% and 24.0 mg/g (DM basis), respectively. High forage diets increased (p<0.001) the concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in ruminal contents and plasma. Monensin supplementation increased (p<0.001) the concentration of trans11-$C_{18:1}$ in ruminal contents, but had no effect on that of cis-9, trans-11 CLA. Concentrations of trans11-$C_{18:1}$ (p<0.019) and cis-9, trans-11 CLA (p<0.022) in plasma increased with dietary monensin levels. Interactions of forage: concentrate ratio and monensin level tended to affect the concentrations of trans11-$C_{18:1}$ (p<0.091) and $C_{18:2n-6}$ (p<0.083) in ruminal contents. Increasing forage levels increased the concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in the rumen. Supplementing with monensin increased the ruminal production of trans11-$C_{18:1}$ and concentrations of trans11-$C_{18:1}$ and cis-9, trans-11 CLA in plasma.

• 한국초지조사료학회지
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• 제8권3호
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• pp.158-164
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• 1988

The growth characteristics and yield per unit area of two cropping systems, corn monoculture and 'wrnsoybean intercropping, were compared and the obtained results were as follows; 1.The two cultivation systems were not significantly different in leaf length, leaf width and length of internode at harvesting time. 2.The weight of stalk and the leaves of a plant were decreased with maturity, while the weight of ear was markedly increased. The patterns of relative ratio of each components were essentialy same in each cultivation systems. In corn monoculture, the percentages of stalk, leaf, and ear at final harvesting stage were 22.9, 13.7, and 63.4 and in corn-soybean intercropping, they were 21.8, 10.9, and 67.3 respectively. 3.Dry matter yield per unit area (kg/lOa) of intercropped corn at yellow stage was similar to that of monocultured corn (1, 483 kg/ 10a vs 1, 509 kg/ 10a). At ripe stage, however, the dry matter yield of intercropped plant was more than that of monocultured (1, 679 vs 1, 660 kg/ 10a). 4.The same pattern was observed in organic matter yield.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.980-989
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• 2005

The influence of treated, extruded, partially expelled soybean meals as undegradable protein and bypass fat sources on lactation performance and ruminal fermentation of dairy cows was studied. Experiment 1: nine cows were used in a replicated 3${\times}$3 Latin square design with each period being 3 wk in duration. Cows were fed 440 g/kg forage and 560 g/kg grain diet with one of three extruded soybean meals fed at 110 g/kg of the diet. The 3 soybean meals were 1) twice-extruded soybean meal (ESM; as a control); 2) lignosulfonate-treated, twice-extruded soybean meal (LSM); and 3) calcium oxide plus lignosulfonate-treated, twice extruded soybean meal (CLSM). Experiment 2: 3 ruminally cannulated cows were used in a 3${\times}$3 Latin square to study the treatment influence on ruminal fermentation characteristics. Feeding treated soybean meal to cows in LSM and CLSM treatments did not improve feed intake, milk yield, or milk composition except that cows fed the LSM and CLSM treatments produced less milk protein compared with the ESM treatment. The proportion of $C_{18:2}$ was greater in milk fat of cows fed CLSM compared with that of cows fed the ESM or LSM treatments. Ruminal pH, ammonia, and total volatile fatty acids were not affected by treatment. An increased proportion of $C_{18:2}$ in milk fat suggests that there is a potential use of calcium salts of fatty acids in protecting the lipid portion of extruded soybean meal and further research is needed to explore this potential with full-fat extruded soybeans not with extruded and partially oil expelled soybeans.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.234-234
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• 2022

Gene-editing is currently one of the most popular technologies in recent years. Development of the new crop using the gene editing have advantage of improved accuracy and efficiency compared with conventional breeding. Soybean (Glycine max L.) is one of the most important crops worldwide used as food and forage. We tried to establish a system for breeding improvement of soybean through gene-editing technology. For the gene-editing system of soybean, i) selection of efficiency gRNA of targeted gene, ii) efficient genetic transformation of the selected gRNA, iii) selection of trans-clean mutant is essential. First of all, we investigated the selection conditions of gRNA with high editing efficiency of targeted gene using isolated protoplast of soybean. Furthermore, we performed the Agrobacterium-mediated genetic transformation of various soybean cultivars. We identified the tissue culture ability in 23 soybean cultivars for genetic transformation of soybean. The six cultivars with high tissue culture ability were selected and confirmed the transgenic plants in four cultivars. Finally, we established a speed-breeding system as a powerful tool for the fast selection of trans-clean mutants from transgenic plants. Our laboratory will provide the valuable system for improvement of soybean by the gene-editing technology.

• 한국초지조사료학회지
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• 제25권1호
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• pp.23-32
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• 2005

This experiment was carried out to determine on the growth characteristics, yield and palatability in mono-cropping(T1; sorghum $\times$ sudagrass hybrid, T2; soybean) and inter-cropping[T3; sorghum $\times$ sudagrass hybrid(1) + soybean(2)]. The results obtained are summarizes follows; In plant length. leaf length, leaf width and stem diameter of sorghum $\times$ sudagrass hybrid(SSH). T3(1) was lower than T1 at 1st cutting time. But in 2nd and 3rd cutting time. T3(1) was higher than T1 Stem diameter of T2 was higher than soybean of T3(2). but plant length, leaf length and leaf width of T2 were higher than T3(1). The leaf ratio of SSH in T3(1) was higher than T1 in the first cutting time, but T3(1) was higher than T1 at 2nd and 3rd cutting time. The number of tiller of T1 was higher than SSH of T3(1) at 1st cutting time, but T3(1) was higher than T1 at 2nd and 3rd cutting time. Dead stubble rate of SSH in T3(1) showed a little lower value than T1. Dry matter and fresh yield of T1 was higher than T2 and T3. But protein yield of T3 was higher than T1 The palatability of 1st cutting time was 100.0. 76.8 and $18.5\%$ on T2, T3 and T1, respectively.

• 한국초지조사료학회지
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• 제10권2호
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• pp.110-114
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• 1990

Although corn is regarded as the most feasible forage corp, its relatively low content of protein is the critical we a kness for animal feeding. Many researches have been carried out to improve protein level in corn forage, however, there are no indicatable results but corn-legume intercropping. Plot test and proximate analysis were fullfill to compare dry matter yield and available nutrients of silage corn mono-culture system with those of corn-legume intercropping system of forage plant and silage. The MO culture system were observed by two stage of maturity, milk stage (Aug. 3), yellow stage (Aug. 24), and obtained following results. 1. Dry matter yields per 10 a at milk stage in corn mono-culture system was 596.2kg and corn-legume intercropping systems were 609.0 kg (corn-cowpea), 591.0 kg (corn-soybean) and 563.1 kg (corn-frenchbean), respectively. And comparable to them, 1508.9 kg (corn mono.), 1482.8 kg (corn-cowpea), 1482.6 kg (cornsoybean), 1379.1 kg (corn-frenchbean) were harvested at yellow stage. 2. The general trends of chemical composition by stages of maturity in corn mono-culture systems were higher than that of corn-legume intercropping system. 3. Crude protein content in corn-legume silages were significantly higher than corn mono-culture at yellow stage, except corn-frenchbean intercropping system. 4. Crude fiber content in corn-legume silage was higher than corn mono-culture silage harvested at yellow stage. 5. Consequently, corn-legume intercropping improved nutritional quality of silage than that of corn-monocultivated silage.