• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.85-116
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• 2005

Gyeryongsan(845m) is located at $36^{\circ}\;18'\;02'\;-36^{\circ}\;23'\;38'$ in latitude, at $127^{\circ}\;11'\;60'-127^{\circ}\;17'\;86'$ in longitude and on Nonsan City and Gongju City in Chungnam Province. Gyeryongsan shows the typical vegetation patterns including the middle area of temperate region represented by the secondary forests of Quercus mongotica and Q. serrata. And we tried to discuss on the distribution and availability of vascular plants including economical plants. The results of plant collection and their investigation from April in 1998 to October in 2002 are as follows : The vascular plants consist of total 684 taxa; 3 hybrids, 12 forms, 84 varieties, 1 subspecies, 584 species, 357 genera, 100 families, 34 orders, 4 classes, 3 subphyla. In this area useful resources plants were 277 taxa$(40.5\%)$ forage source, 261 taxa$(38.2\%)$ edible source, 204 taxa$(29.8\%)$ medicinal source, 80 taxa$(11.7\%)$ ornamental source, 20 taxa$(2.9\%)$ timber source, 3 taxa$(0.4\%)$ industrial raw material source respectively. Also, the Korean endemic plants are 29 taxa($4.2\%$ among total 684 taxa); 5 varieties, 24 species, 27 genera, 20 families. And rare and endangered plants are 2 taxa; Paeonia obovate, Gastrodia elata. Also, the naturalized plants are 31 taxa($4.5\%$ among total 684 taxa and $13.7\%$ among the total naturalized plants in Korea).

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.1
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• pp.37-44
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• 1985

With a view to producing forest trees and forages, the grassland development in the forest has become an important issue in Korea. For better grassland development in the forest, a field research was carried out to investigate the effects of 3 dominant types of seed mixtures (orchardgrass type, tall fescue type, and reed canarygrass type) on the botanical composition, pasture plants composition and dry matter yield of grass-clover mixtures grown under pine trees (shading level; about 50%). As a preliminary test, 3 shading levels (o; full sunlight, 50, and 60%) were treated to compare the yield of pasture in the forest. This experiment was arranged as a randomized block design with 3 replications, and performed at the experimental field in the suburban forest of Suweon, during 1982 to 1984. The results obtained are summarized as follows: 1. As the shading level increases by 0, 50, and 6075, the dry matter yield of forage was decreased by 100 (11.13 ton/ha),81 and 76%, respectively. 2. The total ground cover and percentage of pasture plants were decreased by year, regardless of 3 different dominant types of pasture mixtures. The wild grasses and shrubs, however, tended to be increased by year. 3. Orchardgrass was found to be the only dominant grass showing 80% of all pasture plants, regardless of 3 types of pasture mixtures. But the percentage of tall fescue, reed canarygrass, Kentucky bluegrass and ladino clover were low. 4. Dry matter yield was rapidly decreased to 5075 in the second harvest year, 1984 compared with that of the first harvest year, 1983. In 1983, the yield of orchardgrass type, tall fescue type, and reed canary-grass type was 8.95, 9.05, and 7.93 ton per ha, respectively, while in 1984, the yield was 4.66, 4.57, and 4.38 ton per ha, respectively. 5. From the above results, it is suggested that tile orchardgrass dominant type of pasture mixtures is the most desirable considering forage production and botanical composition, and maintaining productivity for several years is important in the grassland under the trees.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.123-130
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• 2002

A field experiment was carried out to determine the effect of application level of animal manure on the nitrate nitrogen concentration, sugar content and animal intake of forage sorghum $\times$ sudangrass hybrid (Sorghum bicolor (L.) Moench, cv. Pionee. 988) in 1995. The application amount of animal manure were 50, 100 and 150MT in cattle manure, 20, 40 and 80MT in swine manure, and 10, 20 and 40MT/ha in poultry manure. Non-application plot(control) was involved. The nitrate nitrogen concentration was increased with increasing of application level of animal manure(P<0.05). Average nitrate nitrogen concentration was 397, 512, and 609mg/kg at low, medium and high application level of animal manure. The nitrate nitrogen concentration by plant height was 438mg/kg at 50~60m of plant height, 454mg at 100~120cm, and 418mg at 200~220cm. The nitrate nitrogen concentration of stems was 376mg, and significantly higher than that(135mg) of leaves(P<0.05) regardless of animal manure type, and lower parts of stems and leaves were significantly higher than those of upper parts of plants(P<0.05). Average nitrate nitrogen concentration of leaves was 151mg at lower, and 58mg at upper parts of plants, and the concentration of stems was 357mg at lower, 511mg at middle, and 610mg at upper parts of plants. The sugar contents of sorghum $\times$ sudangrass hybrid was decreased with increasing of application level of animal manure(P<0.05). Average sugar content was 4.9, 4.4, and 4.3。 at low, medium and high application level of animal manure. The sugar content by plant height was 3.9。 at 50~60 and 100~120cm of plant height, and 6.1。 at 200~220cm of plant height. Animal intake of sorghum $\times$ sudangrass hybrid was decreased greatly with increasing of application level of animal manure. Average intake was 73.9, 55.7, and 52.3% at low, medium and high application level of animal manure. The intake by animal manure type was 73.7% in cattle, 59.7% in swine and 62.5% in poultry manure.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.3-12
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• 1992

Forage grasses and legumes ar$\varepsilon$ the mam component of livestock diets in Canada. There are over 30 million ha of grassland in Canada and there is a large, undeveloped land base in fringe areas suitable for forage production. The short growing s season limits the grassland farming to the southern p parts of Canada. The win!er season is long and in most parts of Canada cold temperatures, fr$\varepsilon$ezmg, and thawing, and diseases exert sever$\varepsilon$ stress on overwintering forage plants. The development of persistent cultivars is essential for sustained production particularly in the fringe areas with short growmg s$\varepsilon$ason. The seasonality of dry matter production is a result of high growth rates in early summ$\varepsilon$r and low dry matter accumulation in late summer and fall. Innovative management practIces a and cultivars with improved regrowth capacity are n necessary to overcome such skewed production pattern and to extend effiectlVe grazmg season l Improved pasture production is an important part of reducing costs in livestock operations and remaining competitive. It is suggested that applying available technology would increase pasture productivity and reduce d$\varepsilon$pendence on stored feeds thus improving profitability of small producers in particeular. Reducing nutrient losses during harv$\varepsilon$stmg, s storage, and feeding is essential for improved production efficiency during confinement. The devclopment of low cost and labor saving methods of ensiling is critical for improved efficiency and profitability of forage based enterprises Livestock industries must respond to consumer preferences for low fat and cholesterol foods. Research and development of entire production systems is emphasized for dev$\varepsilon$loping viabl$\varepsilon$ enterprises. It is increasingly difficult to secure resources for r$\varepsilon$search, education, and extension, and alliane$\varepsilon$s and cooperation must expand among organizations with interests in forage based livestock systems.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.221-224
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• 1999

A field experiment was carried out to observe the weed control effects of vetch and to evaluate vetch characteristics on clay loam soil in no-till direct-sown rice-vetch cropping systems. The effects of weed control, forage productivity, and N content of vetch plants were investigated. With the progress of plant growth, density of Chinese milk vetch (Astragalus sinicus L.) gradually decreased, but densities of foxtail and other weeds decreased steeply due to the depression by the over-shaded vetch canopy in a no-till direct-sown rice-vetch cropping paddy field. The vetch density in tillage systems was lower than in notillage cropping systems. Lower vetch density occurred with an increase in foxtail density and other weeds. Weed control effect increased by the progress of vetch growth, which indicated that the vetch canopy over-shaded the weeds. Vetch straw was degraded rapidly submergence after with water at the time of wet sowing of rice. Early harvesting of vetch seed resulted in lower seed germination. To acquire enough seedlings without re-sowing, the harvesting of seed should be delayed at least 28 days after the flowering stage in order to ensure the vetch population is sustainable in a no-till direct-sown rice-vetch cropping system. In order to improve the survival of vetch plants, vetch seeds should germinate from the heading .stage to before the full-ripening stage of rice plants. To enhance the percent of over-wintering survival, vetch seeds should germinate no later than the end of October in southern Korea. The dry weight of vetch plants increased with the progress of vetch growth until the flowering stage but N content decreased for 30 days from before the flowering stage (2.9%) until the ripening stage (1.8%). We concluded that Chinese milk vetch could have an effect on weed control before the flowering stage, sustainability without re-sowing of seed annually, and effective green manure for rice pre-crop in no-till direct-sown rice-vetch relay cropping systems.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.237-242
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• 2004

A system for the production of transgenic plants has been developed for tall fescue (Festuca arundinacea Schreb.) via Agrobacterium-mediated transformation of mature seed-derived embryogenic callus. Seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase (HPT), neomycin phosphotransferase II (NPTII) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of $200\mu\textrm{M}$ acetosyringone (AS) in inoculation and co-culture media lead to a increase in stable transformation efficiency. Transformation efficiency was increased when embryogenic calli were co-cultured for 5 days on the co-culture medium. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agyobacterium in the presence of 0.1% Tween20 and $200\mu\textrm{M}$ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and Southern blot analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of tall fescue.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.222-229
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• 1991

This experiment was carried out to determine the effect of improvement of oversown pastures, treated with four nitrogen(N) application levels(0, 100, 200, 300 kg/ha) and three species mixtures (A: orchardgrass+ladino clover; B: orchardgrass+ladino clover+perennial ryegrass; C: orchardgrass+ladino clover+perennial ryegrass+Italian ryegrass). The establishment of perennial ryegrass, Italian ryegrass, and ladino clover were increased with increased N applications, but the ryegrass mixed with orchardgrass and ladino clover decreased the establishment of ladino clover. With the increase of N application levels, orchardgrass, perennial ryegrass and Italian ryegrass in comparison on botanical composition were getting better, while the native plants established already were decreased. The botanical composition of newly sown pasture plants was increased as cutting times progressed. The dry matter(DM) yield of pasture species increased significantly with N application up to 300 kg/ha. There was no significance among mixture treatments, although the DM yield of basic treatment(orchardgrass+ladino clover) was higher than other treatments. However, when compared DM yields on each cutting times, newly oversown pastures in A treatment was shown to be significantly higher only on 2nd cutting time than other treatments(P<0.05). The DM yield of native plants survived in basic species mixture was significantly less than those of other mixtures(P<0.05). Results of this study indicate that for improving oversown pastures, at least 300 kg/ha N application should be applied, and basic mixture (orchardgrass+ladino clover) has more advantage than treatments mixed with ryegrass.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.165-170
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• 2009

A system for the production of transgenic plants has been developed for perennial ryegrass (Lolium perenne L.) via Agrobacterium-mediated transformation. Included in this study were two factors which may affect the gene transfer efficiency: concentrations of acetosyringone (AS, 0 to 300 ${\mu}M$), and co-culture period (1 to 7 days). Both factors were very important to achieve high efficiency gene transformation in the perennial ryegrass. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium in the presence of 100 ${\mu}M$ AS with the culture medium for 5 days. Phosphinothricin resistant calli were developed with into complete plants. GUS histochemical assay, polymerase chain reaction (PCR) and Northern blot analysis of transgenic plants demonstrated that transgenes were integrated into the genome of perennial ryegrass. Using this protocol, it was possible to obtain transformants efficiently for further study.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.6-11
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• 1991

In order to classify the varieties by the characters to evaluating potential winter hardiness was examined using the relationships between autumn growth of seedling plants and the dry weight of plant(DW), and yield components of 1st cutting in 16 varieties of perennial ryegrass grown under individual plant basis.The results are summarized as tollows:1. The dry weight of plant(L)W) was positively correlated with the number of tillers of seedling plants, but negatively correlated with seedling vigors of plants in autumn growth. 2. The dry weight of plant(L)W) indicated positively significant correlations with the dry weight of heading tillers(HDW), number of tillers per plant(NT), number of vegetative tillers(VNT), number of heading tillers(HNT), dry weight of a tiller(WT) and heading rates(HR) of 1st cutting.3. Relative tiller appearance rates(RTAK) corresponded with the seasonal patterns of tillering ability. Thevariety with a low values of relative tiller appearance rates(RTAR) showed vigorous tillering ability inautumn growth, but the variety with a high values of relative tiller appearance rates(KTAR) showedvigorous tillering ability in spring growth.4. The dry weight of plant per relative tiller appearance rates(DW/RTAR) indicated positively significant correlations with the dry weight of plant(DU7, plant length(PL), dry weight of heading tillers(HDW), number of tillers per plant(NT), number of heading tillers(HNrr), dry weight of a tiller(WT) and heading rates(HR) of 1st cutting.5. The varieties were classified into 2 groups by the dry weight of plant per relative tiller appearance rates(DW/RTAR). Thus. Manhattan, P-2, Tonga, Tove, Hella. I'eramo and Fiesta belong to the group of DW/RTAII>l. 0, and Maprima, Caliente, Tempo, Gambit, Magella, Prana. Reveille, Barlet and Bravo belong to the group of DW/RTAR

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.177-183
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• 1993

This study was conducted to obtain the transformed tobacco plants with S. cerevisiae Acid phosphatase gene(PH05) using Agrobacterium tumefaciens and th confirm plant transformation and gene expression. the results obtained were summarized as follows: APase activity of Saccharomyces cereviase NA 87-11A was remarkably showed up as deep red color when assayed by Tohe and Oshima(1974). PH05 fragment, Apase gene, was obtained from pVC727G and the graphically estimated size was about 1.5kb by agarose gel electrophoresis. The sequencing results of 5'end and 3'end of PH05 using dideoxy chain termination method were coinsided with the full length nucleotide already. pBKJ I vector was constructed by isolation of PH05 fragment from pVC727-1 and pBKSI-1 digesred with Sma I and Xba I. Isolated plasmid from transformed A. tumefaciens with constructed pBKJ I when it was electrophoresed with agarose gel. The dosc of tobacco leaf was cocultivated 재소 transformed Agronacterium tumefaciens. Transformed shoots were selected on kanamtcin-containing MS-n/B medium and they were regenerated. The transgenic tobacco plants were elucidated by isolation of genomic DNA and genomic southern hybridization using ${\alpha}-^{32}P$ labelled PH05 fragments. The PH05 in transformed tobacco plants was expressed in leaf, stem and root, and its APase activity was estimated as deep red color by Tohe method.