• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.237-242
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• 2016

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.77-84
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• 2002

The alpine grassland vegetation at the west slope of Cheungseuk-bong on Mt. Paektu was investigated by Penound-Howard's cover-degree method. The floristic composition of the alpine grassland from 1,800m altitude to the top of mountain were 30 species, 2 subspecies, and 3 varieties, and most of the plants were short(height 2∼13㎝) perennial herbs and shrubs. The highest cover degree of the species in the alpine grassland zone were Ligularia jamesii, Veratrum patulum and Thalictrum aquilegifolium (1,800∼2,l00m), Vaccnium uliginosum and Rhododendron aureum (2,100∼2,l00m), and Rhododendron aureum (2,300∼2,600m). Life form of the plants were 7 species of Chamaephyte, 27 species of Hernicryptophyte, and 1 species of Geophyte. Among the presented plants, 19 species were at flowering stage. Trollius hondoemsis, Trollius japonicus, Liguiaria jamesii and Lloydia serotina were peak at flowering.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.57-63
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• 1994

We use a variety of methods to determine the optimum time for havesting corn for silage. In addition, adequate dry matter for silage must be considered along with maturity stage. The objective of this study was to evaluate using the kernel milk line to determine when to harvest com for silage in 1992 on the Livestock Experimental F m , Keiymung Junior College at Keongsan, Keongsangbukdo. Four hybrids were field grown and com plants were harvested at various stages of kernel development so that kemel milk line movement could be analyzed whilc the corn was in the premature stages. As the plants approached maturity, the ears were collected from each of the hybrids and the position of the milk line wa5 noted. Then the whole plants were chopped and the content of DM was determined. The milk line was a readily identifiable feature of maturing com kemels. We focused on the five development \tage\. The fint was "soft dough". The second was "dent". The third wa, "75% milk". and the fourth wa5 "half milk". The half milk occurs when the milk line is positioned falf way down the kemel face. and the final stages win "no milk", milk disappearance as indicators of physiological maturity in maix. Milk free stage of the kemel occurred from I to 3 days prior to black layer having developed. The range for harvesting com for silage occurs a kemels mature from 75% milk to no milk. Position of milk line was easy to see. and can be used as a visible indicator to determine com matunty stage\ and whole plant dry matter. Whole plant dry matter increased with advancing maturity. averaged over hybrids it was 24.1, 25.6. 28.5. 34.6 and 39.0% at soft dough, dent, 75% milk. half milk and no milk. Milk line was more usehl indicator in monitoring corn maturity prior to physiological maturity.ing corn maturity prior to physiological maturity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.4
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• pp.253-259
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• 1992

The esterase isozyme in tissue of wild legume plants were separated by horizontal starch gel electrophoresis. Extracts used in this study were prepared from fully expanded young leaf, cotyledon and radicle of seedling and root-nodule of Glycine sola, Vigna vexillata var. tsuscmensis and Trifoliwn repens. The results are as follows; 1. Each tissue examined had a characteristic banding pattcrn. Number of bands in each species, G. soja, V. vexillata, and T . repens, were 14, 8 and 1 1 bands, respectively. And difference in esterase isozyme bands were greater from tissue to tissue than difference between habitat. 2. Est-I, Est-2. Est-3 and Est-4 in G. soja, Est-I in V. vexillata and Est-l and Est-2 in T. repens showed strong cnzyme activity than other enzyme. 3. Esterase isozyme variation in G. soja and T . repens showed more variety than V. vexillata. This is resulted from many genotypic differences within species. 4. The main enLyme among thc esterase isozyme were Est-I. Est-2, Est-3 and Est-4.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.49-52
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• 2001

conditions for callus induction and plant regeneratin from seeds of lawngrass (Zoysia japonica Steud.) were confirmed in this study. MS (Murashige and Skoog) medium containg 2,4-D 3 or 5mg/l was used for callus induction, and MS medium with different volumes of BA, NAA and kinetin hormones was used to regenerate the plants from induced calli. MS basic medium containing agar with no hormones or kinetin 1.0mg/l and MS basic medium containing gelite and NAA 1.0mg/l were higher for green callus induction. MS medium containing agar and kinetin 1.0mg/l ws highest degree of efficiency for plant regeneration.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.257-264
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• 1997

This study was conducted to assess present situiatioq of oversowing grassland yield and vegetational succession of abondoned since 6 years passed grassland in Wonju. The highest annual h s h weight yield was 28ton/ ha and the lowest was I lton/ha and the grass rate of investigated oversowing grasslands were range from 19.5% to 9lS%. The soil properties of abandoned oversowing grassland, such as soil pH content of organic matter, available phosphorus, exchangeable potasium, calcium, and magnesium were 5.7, 3.88%, 3lppm, O.lme1100g 4.9meI 100g and 2.3me/100g, respectively. The floristic composition of abandoned grassland were composed 26 species of vascular plants. The dominant species based on SDR, of grassland were Miscanthus sinensis var. purpurascens(56.32%). Artemisia feddei(48.62%) and Dactylis glomerata(43.85%). The biological spectra showed the H-$D_1$-$R_5$-e type.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.188-210
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• 2002

Tonggo-san(1066.5m) is located at 36° 53' 00" - 36" 55' 00" in latitude, at 129° 10' 30" - 129° 13' 00" in longitude and on Seo-myeon, Uljin-gun and Subi-myeon, Yeongyang-gun in Gyeongsangbuk-do. Tonggo-san shows the typical vegetation patterns including the middle area of temperate region of Korea represented by the secondary forests of Quercus mongolica and Q. dentata. There was no detailed result on the flora and vegetation of Tonggo-san until now. Therefore, we tried to discuss on the distribution and availability of vascular plants including economical plants. Also, we could discuss the especialized plants and their distributions according to the criteria by the Ministry of Environment. The results of plant collection and their investigation from April in 2001 to November in 2001 are as follows : The vascular plants consist of total 565 taxa; 17 forms, 82 varieties, 466 species, 297 genera, 94 families,37 orders, 4 classes, 2 subphyla. In this area, there were a lot of useful economic plants such as 232 taxa(41%) of edible source, 222 taxa(39％) of forage source, 183 taxa(32%) of medicinal source, and 72 taxa(12%) of ornamental source, 15 taxa(2.6%) timber source, 7 taxa(1.2%) industrial raw material source respectively. Among them, the Korean endemic plants were 22 taxa(3.9％ among total 565 taxa); 1 forms, 2 varieties, 19 species, 22 genera, 16 families. And rare and endangered plants was 1 taxa; Astragatus membranaceus. Also, the naturalized plants were 19 taxa(3.4% among total 565 taxa and 10.4% among the total naturalized plants in Korea).

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.3
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• pp.277-284
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• 2011

To investigate the effects of arbuscular mycorrhizal (AM) symbiosis on N uptake and its assimilation under drought-stressed conditions in white clover, total $^{15}N$ amount and $^{15}N$ amount incorporated into $NO_3^-$, amino acids and soluble proteins were quantified by $^{15}N$ tracing during 7 days of water treatment. Under well-watered conditions, there were no significant effects of AM symbiosis on all parameters analyzed in this study. Drought stress decreased total $^{15}N$ amount both in AM and non-AM plants, with a lower rate in AM plants (-13.8%) relative to non-AM plants (-28.5%) at day 7. Drought significantly increased $^{15}N-NO_3^-$ amount in non-AM plants. The amount of $^{15}N$-amino acids was 1.26-fold and 1.33-fold higher, respectively, in leaves and roots of AM plants compared to those of non-AM ones. Drought decreased the amount of $^{15}N$-soluble proteins in leaves at day 7, with a higher rate in non-AM plants than in AM ones. These results clearly indicate that AM colonization effectively alleviating the decrease in N uptake, amino acids and proteins synthesis caused by drought stress.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.3
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• pp.231-239
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• 1995

Alley cropping field experiment has been carried out for 24 months in lime-stone based dryland farming area of southern Bali to study the chemical composition and nutritive value of 16 provenances of Gliricidia sepium The design was a completely randomized block: arrangement, consisted of 16 treatments (G. sepium provenances) and 6 blocks as replications with 12 plants per provenance. Of the 16 gliricidia provenances, six were from Mexico (M), four were from Guatemala (G), one each was from Colombia (C), Indonesia (I), Nicaragua (N), Panama (P), Costa Rica (R) and Venezuela (V). After 12 months establishment, the gliricidia were lopped at 150 cm height regularly 4 times a year, twice during the 4 months wet season and twice during the 8 months dry season. The highest ash, mineral, total soluble phenol, prussic acid and digestibility were in V1; the highest crude fiber component was in M39; the highest gross energy was in P13, while the highest organic matter was in M35. Based on the highest nutritive constituents, V1, C24 and G14 were ranked first, second and third respectively, while based on the lowest non-nutritive constituents C24, V1 and N14 were ranked first, second and third, respectively. The importance of such provenances as source of nutrients for the ruminant during the dry season were discussed.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.524-529
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• 2014

Two methods of feed preference trials were compared to evaluate the acceptability of 5 fresh foliages: Leucaena leucocephala, Moringa oleifera, Portulaca oleracea, Guazuma ulmifolia, and Brosimum alicastrum that was included as control. The evaluation included chemical analyses and forage intake by rabbits. The first method was a cafeteria trial; 12 California growing rabbits aged 8 wk, allocated in individual cages, were offered the five forage plants at the same time inside the cage, while in the second trial 60 California growing rabbits aged 8 wk, allocated individually, were randomly distributed into 5 experimental groups (n = 12/group); for each group just one forage species was offered at a time. The testing period for each method lasted for 7 d, preceded by one week of adaptation. The results showed that B. alicastrum and L. lecocephala were the most preferred forages while on the contrary G. ulmifolia was the least preferred one by rabbits. The results also revealed that the CV% value for the 2nd method (16.32%), which the tested forages were presented separately to rabbits, was lower and methodologically more acceptable than such value for the $1^{st}$ method (34.28%), which all forages were presented together at the same time. It can be concluded that a range of tropical forages were consumed in acceptable quantities by rabbits, suggesting that diets based on such forages with a concentrate supplement could be used successfully for rabbit production. However, growth performance studies are still needed before recommendations could be made on appropriate ration formulations for commercial use.