• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.237-242
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• 2016

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.2
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• pp.101-108
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• 2012

To determine sulfate uptake and assimilation of various rape cultivars (Brassica napus L.) under different S levels, the activity of ATP sulfurylase, ${SO_4}^{2-}$ uptake and glutathione (GSH) concentrations were measured in different age of leaves. In this study, ten rape cultivars (Mokpo, Tamra, Youngsan, Naehan, Saturnin, Akela, Mosa, Capitol, Pollen, and Colosse) showed various sulfate uptake and assimilation capacity in response to inefficient sulfate supply. Under inadequate sulfate conditions, a greater ATP sulfurylase activity in young leaves was observed in all cultivars compared to that of old leaves. In addition, GSH concentration was considerably increased in young leaves when S supply was declined from 2.0 to 0.2 mM. These results suggested that rape cultivars differ in their capacity to utilize sulfate under limited S conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.2
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• pp.103-107
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• 1989

To determine optimal sowing time of forage rape in southern areas of Korea, forage rape cv. Velox, the highest yielding variety among seventeen introduced varieties of forage rape, was grown under five different sowing times. The results obtained are summarized as follows: 1. Yield components such as plant length, number of branches and number of leaves etc. were highest at the sowing time of Sep. 14 and Sep. 24. 2. Plants sown at Sep. 14 and Sep. 24 also showed highest'fresh and dry matter yield. 3. As plants were grown under later sowing time, they showed higher values in content of crude protein and lower values in contents of crude fiber such as NDF, ADF, cellulose and lignin. 4. There was no relationship between variation of IVDMD and sowing time. Plants sown at Sep. 14 and Sep. 24 showed highest digestible dry matter yields.

• Plant Resources
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• v.7 no.3
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• pp.206-210
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• 2004

In order to find out the optimum harvest (dipping) date combined with sowing date on yield and nutrient quality of forage rye which is suitable at the Southern part of Korea, Paldanghomil variety was grown Sep. 2001 to May 2002 at Sunchon National University, and yield and nutrient quality of plant were observed. As harvest date and sowing date were delayed, the plant length was longest, number of leaves per plant was increased in the time of May 20 clipping. Fresh yield was the heaviest in the time of May 20 clipping and Oct. l0 sowing, and the most dry matter yield was the heaviest in the time of May 20 clipping and Oct. 10 sowing. Content of crude protein was the highest and that of crude fiber such as NDF, ADF, hemicellulose, cellulose and lignin were the lowest in the late time of clipping and sowing. Further more IVDMD was high and dry matter yield and digestible dry matter yield were the highest in the time of May 20 dipping and Oct. 10 sowing. Judging from the results reported above, at optimum harvest (dipping) date combined with sowing date for yield and nutrient quality of forage rye seemed to be the time of May 20 clipping and Oct. 10 sowing.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.225-232
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• 2001

In order to identify the optimum seeding rate of Jeju barnyard grass (Echinochloa crusgalli var. fiurnentacea(Roxb) Wight), 15, 20, 25, 30, 35 and 40 kilograms per a hectare were planted in April 16, 2000, respectively. The growth characterization, the yield and chemical composition were then investigated in June 28(1st cutting) and August 30(2nd cutting), 2000. According to increasing the seeding rate, plant height(155.5 to 162 cm), the yield of fresh forage(61 to 73 MT/ha), dry matter forage(11.9 to 16.9 MT/ha), crude protein(0.9 to 1.6 MT/ha), and TDN(6.1 to 9.7 MT/ha) were gradually increased until the seeding furrow, 35 kg/ha. In 40 kg/ha of seeding furrow, however, those results were decreased than that of the former seeding furrow. The contents of crude protein(7.4 to 10.4%), ether extract(3.3 to 5.2%), nitrogen free extract(46.4 to 47.8%), and TDN(52.1 to 60.4%) were also similar to those of the plant heights and the each yield excepted the seeding furrow, 40 kg/ha. Namely, the each results about the contents increased in 40kg/ha of seeding furrow. According to increasing the seeding rate, the stem diameter, number of leaves, number of withering leaves, and fresh weight were gradually decreased. The contents of crude fiber(30.3 to 27.5%) and crude ash(12.7 to 9.0%) were also decreased These results showed that the optimum seeding rate for the best of the feed production was the seeding furrow, 35 kg/ha.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.3
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• pp.179-186
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• 1989

According to the results from the experiments of selecting a suitable variety of forage rape and comparing productivity and nutrient quality between forage rape and oil seed rape, Velox appeared to be the most suitable variety in terms of productivity and nutrient quality at the southern area of Korea among the varieties used in the experiments. Consequently, Velox was grown under two different cultural methods, individual culture and population culture, and productivity and variation of nutrient quality during the growth period were observed and compared between the cultural methods. The results from the experiment are summarized as follows: 1. Although variations were great in plant fresh weight and plant dry matter weight among plants, individual culture showed significantly higher value in these characters at the 1 % level than population culture. Plant fresh weight and plant dry matter weight were largely dependent upon the amount of branches in case of individual culture. However, in population culture, they were equally dependent upon the amount of branches and main stem. There was no significant difference in dry matter percentage between cultural methods, and main stem showed highest dry matter percentage. 2. Content of crude protein was decreasing gradually as plants continued to grow. Individual culture showed higher content of crude protein than population culture from 90 to 120 days after sowing but vice versa from 180 days after sowing to flowering stage. Contents of fiber such as NDF, ADF, cellulose and lignin was low at the early stage of growth. It was increasing gradually as plants grew older and at the latter stage of growth plants under individual culture showed higher values in contents of fiber. 3. In vitro dry matter digestibility (IVDMD) of both stems and leaves was decreasing gradually as plants grew older. Plants under individual culture showed higher IVDMD of stems than plants under population culture, but no significant difference in IVDMD of leaves was observed between cultural methods.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.6
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• pp.439-442
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• 2001

This study has been conducted from April 25, 1999 to June 25, 1999 in Jeju Province in order to determine the influence of seeding date on growth, yield and chemical composition of Kenaf (Hibiscus cannabinus L.) Delaying the seeding date from April 25 to June 25 decreased the plant height of Kenaf from 278.5 cm to 205.7 cm. As for the number of leaves, number of withering leaves, number of branches, and stem diameter decreased as the seeding date was delayed. As the seeding date was delayed, fresh forage yield decreased from 98.5MT/㏊ to 45.9MT/㏊, dry matter yield from 20.7MT/㏊ to 8.2MT/㏊, crude protein (CP) yield from 2.9MT/ha to 1.3MT/ha and total digestible nutrients (TDN) yield form 1l.6MT/㏊ to 5MT/㏊. However, both leaf yield and stem yield were nearly the same tendency. Delaying the seeding date from April 25 to June 25 increased crude protein contents of leaves from 21.5％ to 24.4％, crude fat contents from 5.2％ to 6.1％, nitrogen free extract (NFE) contents form 39.9％ to 41.2％ and TDN contents from 64.3％ to 69.7％. However, delaying seeding date decreased crude fiber contents from 20.8％ to 17.5％, and crude ash contents from 7.9％ to 7.0％. Based on the these findings, optimum seeding date for forage production of Kenaf seems to be about 25 April in atmospheric phenomena and volcanic ash soils of Jeju island.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.5
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• pp.742-746
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• 1999

The potential of Leucaena leucocephala and Prosopis cineraria leaves as nitrogen supplements ($16g/kg\;W^{0.75}/d$) to improve the utilization of a basal diet of rice straw by goats was assessed in a feeding trial. Rice straw supplementation with forage oats (Avena sativa) was fed as a control diet. DMI, $g/kgW^{0.75}$ of rice straw by goats receiving Leucaena was significantly (p<0.05) higher followed by Prosopis and oat fodder. The supplementation of Leucaena improved the digestibility coefficient of DM, OM, CP, NDF and ADF nutrient density. Goats on Prosopis or oat fodder supplemented straw responded similarly in terms of nutrient utilization. The intake ($g/kgW^{0.75}$) of DCP, TDN and nitrogen balance of goats were significantly higher (p<0.05) when Leucaena was fed. It is suggested that the Leucaena may be a useful N-supplement in areas where livestock are fed poor quality crop residues.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.259-264
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• 1995

Several benefits provided by white clover (Tnifoliurn repens L.) can be elevated as relationship between its morphological and physiological characteristics is clarified. The experiment was done to analyze the relationship between them in the clover with different leaf size. Individual plants of Osceola (large leaf), Grasslands Huia (medium leaf) and Aberystwyth S184 (small leaf) were grown in 15m plastic pot containing a 1:2:1 soil:sand:Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. To measure the cultivar response, plants were sampled for morphological and physiological parameters on the date (0) after final harvest and 1, 3, 7, 14 and 28 days after the final harvest and then their relationship was analyzed. Osceola had greater leaf area per trifoliolate but less no. of leaves per plant to result in similar total leaf area per plant of all cltivars. Stolon length and no. of growing tips per plant declined with smaller leaf size although the result in biomass was reverse. Stolon of all cultivars showed the greatest fluctuation in total nonstructud carbohydrate during the regrowing period but nitrogen concentrations of all their fractions and dinitrogen fixation did similar patterns. Stolon and root of Osceola, moreover, were the highest ones. Biomass, stolon length and total leaf area per plant of all cultivars were positively correlated to carbohydrate concentration of all frauions and dinitrogen fixation. In Osceola relationship between nitrogen concentration of stolon and the characters showed in Osceola was positively done.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.1
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• pp.23-27
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• 1983

This experiment was carried out to show how to make the silage of raddish and Chinese cabbage leaves remained after pickling vegetable in autumn. The moisture of raw materials was controlled by the rice straw and barley bran. The results obtaind were as follows; 1. the silage composed of vegetable leaves 70%, rice straw 20% and barley bran 10% showed the best quality and desirable acid content. 2. Adding barley bran increased the crude protein, crude fat, and crude fiber in the silage. 3. 10% of barley bran made the silage more palatable and also have a good colour.