• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.117-124
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• 2022

In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treatment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Brucella abortus, Bacillus anthracis, Clostridium chauvoei, Clostridium perfringens, Escherichia coli, and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90℃ for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

• Journal of Biosystems Engineering
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• v.43 no.3
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• pp.247-254
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• 2018

Purpose: Although previous studies have performed on-farm evaluations for the control of airborne diseases such as foot-and-mouth disease (FMD) and influenza, disease control during the process of livestock and manure transportation has not been investigated thoroughly. The objective of this study is to predict common patterns of livestock-vehicle movement. Methods: Global positioning system (GPS) data collected during 2012 and 2013 from livestock vehicles on Jeju Island, South Korea, were analyzed. The GPS data included the coordinates of moving vehicles according to the time and date as well as the locations of livestock farms and manure-keeping sites. Data from 2012 were added to Esri software ArcGIS 10.1 and two approaches were adopted for predicting common vehicle-movement patterns, i.e., point-density and Euclidean-distance tools. To compare the predicted patterns with actual patterns for 2013, the same analysis was performed on the actual data. Results: When the manure-keeping sites and livestock farms were the same in both years, the common patterns of 2012 and 2013 were similar; however, differences arose in the patterns when these sites were changed. By using the point-density tool and Euclidean-distance tool, the average similarity between the predicted and actual common patterns for the three vehicles was 80% and 72%, respectively. Conclusions: From this analysis, we can determine common patterns of livestock vehicles using previous year's data. In the future, to obtain more accurate results and to devise a model for predicting patterns of vehicle movement, more dependent and independent variables will be considered.

• Journal of Life Science
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• v.30 no.11
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• pp.947-955
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• 2020

The foot-and-mouth disease virus (FMDV), a member of the Aphthovirus genus in the Picornaviridae family, affects wild and domesticated ruminants and pigs. During replication of the FMDV RNA (ribonucleic acid) genome, FMDV-encoding RNA polymerase 3D acts in a highly location-specific manner. This suggests that specific RNA structures recognized by 3D polymerase within non-coding regions of the FMDV genome assist with binding during replication. One such region is the cis-acting replication element (CRE), which functions as a template for RNA replication. The FMDV CRE adopts a stem-loop conformation with an extended duplex stem, supporting a novel 15-17 nucleotide loop that derives stability from base-stacking interactions, with the exact RNA nucleotide sequence of the CRE producing different RNA secondary structures. Here, we show that CRE sequences of FMDVs isolated in Korea from 2010 to 2017 exhibit A and O genotypes. Interestingly, variations in the RNA secondary structure of the Korean FMDVs are consistent with the phylogenetic relationships between these viruses and reveal the specificity of FMDV infections for particular host species. Therefore, we conclude that each genetic clade of Korean FMDV is characterized by a unique functional CRE and that the evolutionary success of new genetic lineages may be associated with the invention of a novel CRE motif. Therefore, we propose that the specific RNA structure of a CRE is an additional criterion for FMDV classification dependent on the host species. These findings will help correctly analyze CRE sequences and indicate the specificity of host species for future FMDV epidemics.

• Korean Journal of Poultry Science
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• v.46 no.2
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• pp.127-136
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• 2019

Three strains of infectious bronchitis viruses (IBVs), designated VNUA3, VNUA8 and VNUA11, were isolated from diseased/infected chickens in Hanoi, Thainguyen, and Haiphong provinces of Vietnam. These birds had received a live IBV vaccination but still suffered from infectious bronchitis. VNUA3, VNUA8 and VNUA11 harbor cleavage sites (RRTGR, HRRRR, and HRRKR, respectively) within the S protein. A BLASTN search revealed that the S gene of VNUA3, VNUA8, and VNUA11 showed the highest nucleotide identity with those of IBV strains CK/Italy/I2022/13, CK/CH/LHLJ/08-6, and GX-NN120084, respectively. Phylogenetic analyses based on the S gene nucleotide sequences revealed that VNUA3, VNUA8 and VNUA11 clustered with Q1-like, QX-like and TC07-2-like genotypes, respectively, and were closely related to reference IBV strains from China. However, the Vietnam IBVs showed high divergence from vaccine strains 4/91 and Ma5, which are used in the Vietnamese farms from which the isolates were obtained. Taken together, these results indicate that at least three genotypes of IBV are circulating among chickens in North Vietnam. This is the first report of the molecular epidemiology of IBV in Vietnam.

• Korean Security Journal
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• no.42
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• pp.229-249
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• 2015

As the post-cold war era and globalization go on, national security problems which were not traditional national security problems such as terrorism, crime, environmental disasters, economic crises, cyber-terrorism, diseases, and energy problems threat humanity and nations and demands changes. Also, with the change, the concept of "big government" has emerged as the role of nation expanded. The modern society sees every country change from small government to big government in order to realize the establishment of welfare state. A comprehensive interpretation of security is needed in order to comprehensive protection of citizens beyond outside invasion such as crime, new disaster, terrorism. In Korea, incidents such as Cheonan-Ham, Yeonpeyong-Do, foot-and-mouth disease, Ddos terrorism, pirates hostages, mad cow disease, AI are happening and the humanitarian support for North Korea and the summit of South and North Korea are at a standstill. Also, National emergency management system, comprehensive emergency management center, countrol tower, national security system, cooperation with citizens, establishment of legal and institutional system are needed. The importance of this research is on the reestablishment of new national security and emergency management system according to the comparison between the national security and counter-terrorism system of Korea and that of the United States which is a leading country in this field. Also, the establishment of national emergency management act is needed as a statute for effective function as currently various laws and administrative organizations are dispersed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.353-362
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• 2018

The national loss caused by the periodic livestock epidemic is very large. In addition, vehicle movement is the main cause of livestock epidemics in Korea. In this context, this study analyzed the relationship between the degree centrality of livestock facilities and the outbreak of infectious diseases. For this purpose, a livestock vehicle movement network was constructed using the facility entrance data provided by KAHIS. Afterwards, the centrality index was derived for each facility in the vehicle movement network and the mean centrality index of the epidemic and non-epidemic facilities were compared. The analysis results are summarized as follows. First, the degree centrality of epidemic facilities is significantly greater than non-epidemic facilities. As a result of the analysis of the entire period data and the period-based data, in most data, the degree centrality of facilities where livestock infectious diseases occurred was significantly greater than most non-occurrence facilities. Second, in the entire period data, the difference in degree centrality between the epidemic and non-epidemic facilities was smaller for HPAI than for FMD. On the other hand, no significant difference was found in the results of the analysis according to the divided period. The policy implications of the results are as follows. First, proactive management of facilities based on centrality is needed. Second, in the case of cloven-hoofed animal facilities, it is more urgent to introduce a management policy based on the degree centrality.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.189-199
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• 2019

The purpose of this study is to derive spatial area(preventive zone) where the movement of livestock vehicles occurs frequently. For this purpose, this study used 6 periods facility entrance data provided by KAHIS. This data was converted into vehicle movement data between livestock facilities and aggregated into administrative district units. The R-mode factor analysis was performed on the constructed OD data, and the region extracted by the same factor was judged as one region. The results of the analysis are summarized as follows. First, the factor analysis of 6 periods data showed 16 ~ 18 factors, and the derived factors explained 63 ~ 68% of the total variance. Second, based on the factors that were derived, Jeonam coastal area, Jeonnam area, Jeonbuk area, Chungnam coastal area, Gyeongnam area, northern Gyeongbuk area, Yeongnam costal area were found to be stable, with little change over time. On the other hand, Chungbuk area, Gangwon area, Seoul metropolitan area are relatively volatile areas. Third, 13 areas were derived by combining data from six periods.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1062-1062
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• 2001

The concept of “precision agriculture” or “site-specific farming” is usually confined to the fields of soil science, crop science and agronomy. However, because plants grow in soil, animals eat plants, and humans eat animal products, it could be argued (perhaps with some poetic licence) that the fields of feed quality, animal nutrition and animal production should also be considered in this context. NIR spectroscopy has proved over the last 20 years that it can provide a firm foundation for quality measurement across all of these fields, and with the continuing developments in instrumentation, computer capacity and software, is now a major cog in the wheel of precision agriculture. There have been a few giant leaps and a lot of small steps in the impact of NIR on the animal world. These have not been confined to the amazing advances in hardware and software, although would not have occurred without them. Rapid testing of forages, grains and mixed feeds by NIR for nutritional value to livestock is now commonplace in commercial laboratories world-wide. This would never have been possible without the pioneering work done by the USDA NIR Forage Research Network in the 1980's, following the landmark paper of Norris et al. in 1976. The advent of calibration transfer between instruments, algorithms which utilize huge databases for calibration and prediction, and the ability to directly scan whole grains and fresh forages can also be considered as major steps, if not leaps. More adventurous NIR applications have emerged in animal nutrition, with emphasis on estimating the functional properties of feeds, such as in vivo digestibility, voluntary intake, protein degradability and in vitro assays to simulate starch digestion. The potential to monitor the diets of grazing animals by using faecal NIR spectra is also now being realized. NIR measurements on animal carcasses and even live animals have also been attempted, with varying degrees of success, The use of discriminant analysis in these fields is proving a useful tool. The latest giant leap is likely to be the advent of relatively low-cost, portable and ultra-fast diode array NIR instruments, which can be used “on-site” and also be fitted to forage or grain harvesters. The fodder and livestock industries are no longer satisfied with what we once thought was revolutionary: a 2-3 day laboratory turnaround for fred quality testing. This means that the instrument needs to be taken to the samples rather than vice versa. Considerable research is underway in this area, but the challenge of calibration transfer and maintenance of instrument networks of this type remains. The animal world is currently facing its biggest challenges ever; animal welfare, alleged effects of animal products on human health, environmental and economic issues are difficult enough, but the current calamities of BSE and foot and mouth disease are “the last straw” NIR will not of course solve all these problems, but is already proving useful in some of these areas and will continue to do so.

• Herbal Formula Science
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• v.9 no.1
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• pp.35-82
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• 2001

In the Encyclopedia Medica Koreana(Dongeuibogam), I have researched 245 prescriptions in which Panax Ginseng plays an important role. And I have got the following results. The healing scope and frequency of ginseng-mainly-included prescriptions are Child Part 29(11.83%), Violent Cough Part 23(9.38%), Sick-by-Cold Part 21(8.57%), Oncosis Part 16(6.53%), Overwork Part 14(5.71%), Gynecologic Part 14(5.71%), Internal Part 13(5.3%), Apoplexy Part 11(4.48%), Mind Part 10(4.08%) and Fecal Part 10(4.08%) prescriptions. And also each of Nausea Part, Anger Part, and Spirit parts has the same 5 (2.04%) prescriptions. And each of Qi Part, Diabetes Meatus Part, Malaria Part, and Humoral Part has 4(1.63%) prescriptions. And each of Foot Part, Choleraic Part, Genital Part, Blood Part, and Voice Part has 3 (1.2%). All of these prescriptions cover 88.88%. And besides listed parts above, Panax Ginseng is all used in 48 Parts: Body-Mind Part. Mouth-Tongue Part, Breast Part, Muscle Part, Swelling Part, Urine Part, Epidermis Part, Heat Part, Anus Part, Stomach Part, Eye Part, Laryngopharynx Part. Uterus Part" Heavy Stomach Part, Head Part, Pulse Part, Hair Part, Navel Part, Emetic Part, Costal Part, Edema Part, Vomiting Part, Superstitious Part, and Cardiac Part, etc. Of the prescriptions in which Panax Ginseng plays an important role, the most representative diseases, which more than 86.8% prescriptions cure, are shock, numbness from cold, Taeeum disease, oncosis, overwork, sick from eating, numbness of extremities, diarrhea, tachycardia, forgetfulness, nausea, heat from kidney, nocturnal emission, short breath, diabetes meatus, malaria, sweating, sweating overnight, beriberi, cholera, insomnia from enervation, sialitis, navel pain, hemorrhage, and loss of voice. The pathology of the prescriptions in which Panax Ginseng plays an important role is divided into the organ problems, six natural factors, seven extreme feelings, unbalanced humoral status, overwork, and, unbalance of qi and blood. Spleen, heart, and uterus is the main cause of organ problems; wind and cold are the main cause of six natural factors; heavy humors are the main cause of unbalanced humoral status; the stasis of seven feelings are the main cause of seven extreme feelings; the lack of stamina and overwork are the main cause of the overwork; the lack of qi, the lack of blood, and, the lack of qi and blood are the main cause of the unbalance of qi and blood. After I have researched the contents of the prescriptions in which Panax Ginseng plays an important role, I could understand the addition of the different prescriptions, combination of medicines, and the role of medicine groups associated with Panax Ginseng. So from now on, the results I have got could be used as the data which show the theoretical basis on the prescriptions.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.241-247
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• 2012

Many infectious diseases have emerged or re-emerged during the past 50 years in South Korea. There were three outbreaks of foot and mouth disease (FMD) in South Korea between January 2010 and March 2011. Over 3.45 million animals were slaughtered (33.3% of the existing pigs, 8.4% of dairy cows and 3.4% of cattle). To select optimal degradation agents of animal cadavers, degradation rates and fertilizer components of pig cadavers were investigated using hydrogen chloride (HCl), potassium hydroxide (KOH) and sodium hydroxide (NaOH) hydrolysis methods. Degradation rates of pig cadavers using HCl, KOH and NaOH were 81.1, 82.8 and 91.6%, respectively. Total nitrogen (T-N) concentration in degradation solution of pig cadavers using KOH hydrolysis method was higher than that in NaOH and HCl hydrolysis methods. Total phosphorus ($P_2O_5$) concentrations in degradation solution of pig cadavers in all hydrolysis methods ranged 0.14 ~ 0.28%. Total potassium ($K_2O$) concentration for KOH hydrolysis method was higher than that for other hydrolysis methods. The concentration of T-N and $K_2O$ in degradation solution of pig cadavers by KOH hydrolysis method were higher than that in NaOH and HCl hydrolysis methods. Thus, to recycle animal cadavers in agriculture, the optimal degradation agent for hydrolysis was KOH.