• Biomedical Science Letters
• /
• v.26 no.4
• /
• pp.302-309
• /
• 2020

Human Coxsackievirus B5 (HuCoxV-B5) infection has been associated with various diseases such as myocarditis, aseptic meningitis, hand-foot-and mouth-disease, and insulin-dependent diabetes. HuCoxV-B5 is a virus transmitted through the fecal-oral route and is detected in clinics, aquatic environments, food, shellfish, etc. and is one of the more important viruses in public health because of its incidence rate reported worldwide. In this study, a combination of SYBR Green-based real-time PCR primers for molecular diagnosis including monitoring of HuCoxV-B5 was selected and the optimal reaction conditions were established. Compared with the previously reported TaqMan probe-based real-time PCR method, assessments including a sample applicability test were performed. Results showed that the real-time PCR method developed in this study was suitable for a molecular diagnostic technique for detecting HuCoxV-B5. This study is expected to contribute to efforts in responding to safety accidents in public health because the proposed method facilitates rapid diagnosis of clinical patients. It can also be used as a specific monitoring tool of HuCoxV-B5 in non-clinical areas such as aquatic environments among others.

• Journal of Korean Society of Environmental Engineers
• /
• v.36 no.10
• /
• pp.679-684
• /
• 2014

Many burial sites were constructed to suppress the spread of foot and mouth disease during outbreak. Defected burial sites were removed when leachate leak is presumed and carcasses were moved to the circular storage tanks. However, carcasses were not decomposed possibly due to low water content, low microbial activities, and poor mixing. In this research, storage tank containing carcasses in it was modified to bioreactor to accelerate stabilization. Liquids with nutrients were added and circulated to maintain the optimum water content while extraneous microorganisms were augmented. Settlement was used as the primary index for assessing stabilization rate, and the consolidation theory was utilized to estimate the expected final settlement. 30% of carcasses is expected to be decomposed and removed from the storage tank for five years of bioreactor operation.

• Annual Conference of KIPS
• /
• 2018.05a
• /
• pp.267-270
• /
• 2018

구제역으로 인하여 국내 축산업계 및 관련 산업분야는 매년 막대한 피해를 입고 있다. 구제역과 관련한 다양한 학술적 연구들이 현재 진행되고는 있으나, 구제역의 발병에 따른 사회적 파급효과에 관한 공학적 분석 연구는 매우 제한적이다. 본 연구에서는 구제역에 관한 일반 시민들의 감성적 반응을 텍스트 마이닝 방법론을 사용하여 분석하는 체계적인 방법론을 제안한다. 제안하는 시스템은 먼저, 트위터에 게시된 트윗 중 구제역과 관련된 데이터를 수집한 후, 감성사전을 기반으로 극성탐지 과정을 거친다. 둘째, 토픽 모델링의 대표적인 기법 중 하나인 LDA를 활용하여 트윗으로 부터 키워드들을 추출하고, 추출된 키워드들로부터 극성별 동시출현 키워드 네트워크를 구성한다. 셋째, 키워드 네트워크을 통해 각 구간별 구제역의 사회적 파급효과를 분석한다. 사례 분석으로써, 2010년 7월부터 2011년 12월까지 국내에서 발생한 구제역에 관한 일반 시민들의 감성적 변화를 분석하였다.

• Journal of Korea Multimedia Society
• /
• v.22 no.2
• /
• pp.136-145
• /
• 2019

The crowded environment of a domestic pig farm is highly vulnerable to the spread of infectious diseases such as foot-and-mouth disease, and studies have been conducted to automatically analyze behavior of pigs in a crowded pig farm through a video surveillance system using a camera. Although it is required to correctly separate occluding pigs for tracking each individual pigs, extracting the boundaries of the occluding pigs fast and accurately is a challenging issue due to the complicated occlusion patterns such as X shape and T shape. In this study, we propose a fast and accurate method to separate occluding pigs not only by exploiting the characteristics (i.e., one of the fast deep learning-based object detectors) of You Only Look Once, YOLO, but also by overcoming the limitation (i.e., the bounding box-based object detector) of YOLO with the test-time data augmentation of rotation. Experimental results with two-pigs occlusion patterns show that the proposed method can provide better accuracy and processing speed than one of the state-of-the-art widely used deep learning-based segmentation techniques such as Mask R-CNN (i.e., the performance improvement over Mask R-CNN was about 11 times, in terms of the accuracy/processing speed performance metrics).

• Korean Journal of Pharmacognosy
• /
• v.47 no.2
• /
• pp.137-142
• /
• 2016

Enterovirus is a common cause of several severe diseases such as myocarditis, hand-foot-mouth disease, and meningitis in children and adult. There are many try to develop new antiviral drug for direct treatment in virus infection. However, synthetic chemical antiviral drug is not working. To overcome this limitation, we examined plant extracts. The antiviral effect of plant extracts was screened by HeLa cell survival assay in coxsackievirus B3 (CVB3) infection. We observed a strong antiviral effect of Poria cocos extract in a dose-dependent manner (1 mg/ml~0.01 mg/ml). P. cocos extract (1 mg/ml) treatment was dramatically decreased virus protease 2A induced eIF4G-I cleavage and virus capsid protein VP1 production. CVB3 positive and negative strand RNA amplification were significantly reduced in P. cocos extract treatment. P. cocos extract completely blocked early time activation of ERK and AKT activity in CVB3 infection. Taken together these data indicate that the treatment of P. cocos extract strongly inhibit CVB3 replication. Poria cocos extract may possible to developed as a therapeutic agent for enterovirus.

• Korean Journal of Veterinary Service
• /
• v.42 no.1
• /
• pp.39-42
• /
• 2019

Tetanus is an acute, often fatal, and infectious disease of all species of domestic animals caused by the neurotoxin of Clostridium tetani (C. tetani). This disease is usually known to develop after microbial contamination in the deep or penetrating wound sites. In February 2017, a farmer who was raising 76 cows injected foot and mouth disease vaccine to three or more cows with one syringe. Their clinical symptoms were observed 2 to 16 days after the vaccination. The initial symptoms were stiffness, rigidity of the neck and limbs, pricked ears, and prolapse of the third eyelid. Subsequently, there was recumbency with extension of the limbs, convulsions and opistotonus and the affected 20 cows were all died. Two dead cows were submitted to Animal and Plant Quarantine Agency for disease diagnosis. At necropsy, a focal edematous abscess of 15 to 20 cm in diameter was grossly observed in the subcutaneous and intramuscular tissue of scapular region and filled with a large amount of greenish pus. The feed was full in oral cavity and slightly observed in the trachea and lobes of lung. Histopathologically, focal granulomatous nodules with eosinophilic materials in the tissue were observed. In the lung, aspiration pneumonia and gram (+) bacteria were seen. The C. tetani was isolated in samples anaerobically cultured using reinforced clostridial medium and identified by PCR. To our knowledge, no previous outbreak of tetanus in cattle has affected such a high number of animals; neither has it been associated with misuse of the same syringe and needle to administer multiple individuals.

• Proceedings of the Korea Contents Association Conference
• /
• 2015.05a
• /
• pp.223-224
• /
• 2015

구제역은 소, 돼지 등 발굽이 두 개로 갈라진 가축들에게 감염을 유발하는 전염성이 매우 높은 바이러스성 질병이다. 구제역 감염 시 입 주변, 구강 내, 코, 발굽사이 등에 수포가 생기며 고열과 식욕이 저하되어 심하게 앓거나 죽게 되는데, 강한 감염 전파력을 가졌음에도 치료제가 없고, 감염확인 즉시 확산 방지를 위한 살 처분만이 이루어지고 있다. 따라서 무엇보다도 빠른 감염여부 진단이 중요하다. 현재까지 구제역을 진단하는 방법으로는 감염 된 가축의 혈액에서 구제역 항원 단백질에 대한 항체형성 여부를 확인하는 항체진단법과 수포액과 같은 체액을 채취하여 세포배양을 통한 구제역 바이러스 분리방법이 있지만 두 가지 모두 짧은 잠복기를 갖는 구제역 바이러스를 빠른 시간 내 진단하기는 어렵다. 따라서 본 연구에서는 보다 빠른 구제역 진단 키트개발을 위해 NCBI Pubmed를 이용하여 구제역바이러스가 가지는 6개의 주요 단백질을 확인하였고, NCBI BLAST를 이용하여 6개의 단백질 중 구제역 바이러스에 특이적인 항원 단백질 peptidase C28을 선정하였다. 선정된 단백질의 아미노산 서열을 이용하여 IEDB analysis resource를 통해 peptidase C28의 epitope 부위를 예측하였다. 예측 된 부위의 아미노산 서열을 NCBI BLAST에서 정상 동물과 비교하여 구제역바이러스 특이 항원 단백질 epitope peptide를 최종 선정하였다. 이를 이용한 구제역 바이러스 진단키트 제작은 보다 빠른 진단을 통해 감염 확산을 조기에 차단하고 경제적 손실과 피해를 최소화 할 수 있다.

• Annual Conference of KIPS
• /
• 2016.10a
• /
• pp.330-333
• /
• 2016

최근 구제역의 발생으로 인해 농 축산업계 및 관련 산업분야에 막대한 피해를 야기함에 따라, 구제역의 발병에 따른 다양한 사회적 파급효과의 분석이 필요하다. 본 논문에서는 온라인 뉴스를 대상으로 텍스트 마이닝 방법들을 사용하여 구제역으로 인한 경제적, 환경적, 그리고 정책적 파급효과를 분석하는 공학적 방법론을 제안한다. 제안하는 시스템은 먼저, 구제역 관련 온라인 뉴스를 수집한 후, 토픽 모델링의 대표적인 방법 중 하나인 LDA(Latent Dirichlet Allocation)를 활용하여 뉴스 기사로부터 키워드들을 추출한다. 둘째, 추출된 키워드들로부터 구제역으로 인한 파급효과의 분석을 위해 동시출현 키워드 네트워크를 구성한다. 셋째, 키워드 네트워크 타임라인을 통해 각 파급효과들의 변화를 분석한다. 마지막으로, 사례분석을 통해 2010년 7월부터 2011년 12월까지 한국에서 발생한 구제역으로 인한 사회적 파급효과의 분석을 수행하였다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2014.05a
• /
• pp.295-298
• /
• 2014

In recently, a climatic change(such as subtropical climate and frequent unusual high temperature) and the open-trade policies of agricultural & livestock products are increasing the outbreak risk of highly pathogenic avian influenza(HPAI) and foot and mouth disease(FMD), and accordingly the socio-economic damage and impacts are also increasing due to the cases such as damage from the last 5 times of FMD outbreak(3,800 billion won), from 10 years public control cost of Pine Wilt Disease (PWD)(238.3 billion won), and from the increased invasive pests of exotic plant like isoptera. Therefore, the establishment of new operation strategy of IoT(Internet of Things) based satellite early warning system(SEWS) for plants and animals as a subsystem of national disaster response and management system is being required, where the forensic technology & measures should be applied as a government policy to estimate the post compensation and to carry out the legal responsibility.

• Journal of the Society of Disaster Information
• /
• v.17 no.2
• /
• pp.275-288
• /
• 2021

Purpose: The purpose of this study is to analyze the characteristics of animal carcasses treatment and quarantine management of infectious diseases of livestock, and to present the effectiveness of quarantine and follow-up management of livestock infectious diseases. Method: Actually, a livestock epidemic occurred and the burial site and burial management facility that treated livestock carcasses were visited on-site to analyze the management status and problems. Result: The burial method of livestock outbreaks of livestock infectious diseases and the long-term follow-up management accordingly requires a large amount of manpower and budget to be spent. For example, it can be seen that it is an inefficient quarantine system. Conclusion: It is necessary to review plans for the establishment of integrated livestock infectious animal carcasses treatment facilities at the level of metropolitan cities, districts, and municipalities with the government, where there is no fear of secondary infectious disease transmission and no follow-up management is required.