Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effect of Poria cocos Extract to Inhibit Enterovirus Replication

적복령 추출물의 심근염 유발 엔테로바이러스 증식 억제 효과

  • Han, Jae-Young (Department of Biomedical Science, Jungwon University) ;
  • Kim, Jin Hee (College of Herbal Bio-industry, Daegu Haany University) ;
  • Lim, Byung-Kwan (Department of Biomedical Science, Jungwon University)
  • 한재영 (중원대학교 의생명과학과) ;
  • 김진희 (대구한의대학교 한방산업대학) ;
  • 임병관 (중원대학교 의생명과학과)
  • Received : 2016.02.11
  • Accepted : 2016.05.13
  • Published : 2016.06.30
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Abstract

Enterovirus is a common cause of several severe diseases such as myocarditis, hand-foot-mouth disease, and meningitis in children and adult. There are many try to develop new antiviral drug for direct treatment in virus infection. However, synthetic chemical antiviral drug is not working. To overcome this limitation, we examined plant extracts. The antiviral effect of plant extracts was screened by HeLa cell survival assay in coxsackievirus B3 (CVB3) infection. We observed a strong antiviral effect of Poria cocos extract in a dose-dependent manner (1 mg/ml~0.01 mg/ml). P. cocos extract (1 mg/ml) treatment was dramatically decreased virus protease 2A induced eIF4G-I cleavage and virus capsid protein VP1 production. CVB3 positive and negative strand RNA amplification were significantly reduced in P. cocos extract treatment. P. cocos extract completely blocked early time activation of ERK and AKT activity in CVB3 infection. Taken together these data indicate that the treatment of P. cocos extract strongly inhibit CVB3 replication. Poria cocos extract may possible to developed as a therapeutic agent for enterovirus.

Keywords

References

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