• Applied Biological Chemistry
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• 제26권2호
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• pp.110-118
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• 1983

병(病)든 콩의 줄기와 뿌리에서 분리(分離)한 bacteria균주(菌株) SAF-C는 운동성(運動性)이 있으며 동정(同定)한 결과(結果) Agrobacterium radiobacter임이 밝혀졌다 또 anilineblue 방법(方法)으로 이 균주중(菌株中) 수용성(水溶性) 다당(多糖)만을 거의 완전(完全)하게 생산(生産)하는 균주(菌株)를 선별(選別)하고 SAF-C.W라 하였다. 진탕배양에서 SAF-C.W의 높은 산성(酸性), 수용성다당(水溶性多糖)의 생산조건(生産條件)을 찾기 위하여 본 실험(實驗)이 행(行)하여졌다. 많은 양(量)의 다당(多糖)이 glucose 4%, $CaCO_3$ 0.3% 질소원(窒素源)으로 yeast extract, $(NH_4)_2HPO_4$ 및 distillers dried solubles(D.DS.)을 0.5% 포함(包含)하는 각각의 배지(培地)에서 생산(生産)되었으며, 그 생산율(生産率)은 22.9%, 10%, 9.6%이었다. 또 glucose 3%, D.D.S. 0.4% $K_2HPO_4$ 0.5%, $MgSO_4{\cdot}7H_2O$ 0.01%의 조성(組成)을 가지는 배지(培地)에서 다당수득율(多糖收得率) 25%를 기록(記錄)하였다.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.449-456
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• 2023

Sugar or dextrose increases the cost of production of xanthan gum by Xanthomonas campestris. Brewers' Spent Grain (BSG) was chosen as a source of fermentable sugars. BSG is a significant industrial by-product generated in large quantities from the breweries. Primarily used as animal feed due to its high fiber and protein content, BSG holds great potential as an economically and ecologically sustainable substrate for fermenting biomolecules. This study explores BSG's potential as a cost-effective carbon source for producing xanthan, utilizing Xanthomonas campestris NCIM 2961. An aqueous extract was prepared from BSG and inoculated with the bacterium under standard fermentation conditions. After fermentation, xanthan gum was purified using a standard protocol. The xanthan yield from BSG media was compared to that from MGYP media (control). The fermentation parameters, including pH, temperature, agitation and duration were optimized for maximum xanthan gum yield by varying them at different levels. Following fermentation, the xanthan gum was purified from the broth by alcoholic precipitation and then dried. The weight of the dried gum was measured. The obtained xanthan from BSG under standard conditions and commercial food-grade xanthan were characterized using FTIR. The highest xanthan yields were achieved at 32 ℃, pH 6.0, and 72 h of fermentation at 200 rpm using BSG media. The FTIR spectra of xanthan from BSG media closely resembled that of commercial food-grade xanthan. The results confirm the potential of BSG as a cost-effective alternative carbon source for xanthan production, thereby reducing production costs and solid waste.

• 농업과학연구
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• 제50권3호
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• pp.459-467
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• 2023

Because an irradiation of 405 nm violet light could have a strong energy, it was used to be sterilized against various microbes in the indoor air condition or fresh food. Escherichia coli is a representative bio-pollutant in the indoor air-borne bacteria, and a hygienic microbe in the horticultural food. This study evaluated the inactivation influences on E. coli DS5α after exposure to 405 nm violet-light (VL) by investigating irradiating time, and the vertical and horizonal distance from light source. The illumination of 405 nm VL was inversely proportional to the distance from the VL source. E. coli DS5α on nutrient agar (NA) was inactivated approximately 50% more than the control when irradiated at 65 cm from 405 nm VL for 3 hours. When compared to the control, E. coli DS5α was inactivated approximately 50% within 70 cm from 405 nm VL for 3 hours. As it was irradiated for 3 hours 70 cm away from 405 nm VL, the horizonal distance from the point was negatively correlated to the inactivation of E. coli DS5α. These results indicated that the inactivation of E. coli DS5α grown on NA medium needs to be irradiated with 405 nm within 70 cm from the light source for 3 hours.

• Preventive Nutrition and Food Science
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• 제8권3호
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• pp.225-229
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• 2003

Clarified apple, carrot and orange juices were prepared using ultrafiltration and their single and blend juices were further concentrated using ultrafiltration, freeze-drying, and rotary evaporation. Effect of concentration methods on the quality of concentrated single juices and juice blends was investigated. Turbidity values of samples concentrated by evaporation were significantly higher than those prepared by ultrafiltration and vacuum freezing regardless of juice source (i.e., apple, orange or carrot) or blending (p<0.05). The highest soluble solids contents were obtained for the samples concentrated by evaporation process. Concentrated apple juice contained significantly higher amount of vitamin C and soluble solids than concentrated orange and carrot juices regardless of concentration methods (p<0.05). For blended samples, no direct relationships between blend ratio and total amount of vitamin C were found; however, samples contained more apple juice showed the highest value of soluble solids regardless of concentration methods.

• 대한수의학회지
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• 제36권2호
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• pp.463-470
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• 1996

The red yeast Phaffia rhodozyma, which contains astaxanthin(3, 3'-dihydroxy-$\beta$, $\beta$-carotene-4, 4'-dione) as its primary carotenoid, was tested as a dietary pigment source for egg yolks of laying hens. When the yeast was fed to laying hens at several concentrations, the intensity of redness in egg yolks was dependent on the yeast concentration in the feed and the deposition period. Addition of P rhodozyma in feed did not cause any visible adverse effect on laying hens.

• Journal of Nutrition and Health
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• 제24권1호
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• pp.20-29
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• 1991

This study was carried out to compare the protein quality of lamb and beef meat. by feeding to growing rats. Sixty weanling rats, 30 males and 30 females, were blocked into 12 groups(6 gruops of males and 6 groups of females). They were fed casein. beef, or lamb as a protein source at two levels, 6 and 15%, for 5 weeks. The amount of food intake. food efficiency ratio, protein efficiency ratio. body weight gain. and the weights of skeletal muscles and liver were measured. Nitrogen retention, protein content in the liver and skeletal muscles, and the levels of protein and cholesterol in the serum were also assayed. Summarzing the results, there were no significant differences between lamb and beef on the growth and nitrogen utilization in the rats fed same percentage of protein diet. However. rats fed 15% protein diet showed significantly higher growth rate than those fed 6%. Therefore, it can be concluded that lamb is as good a protein food as beef in terms of protein quality.

• 한국식품영양학회지
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• 제11권6호
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• pp.612-616
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• 1998

Optimal media and cultural conditions for the production of red pigment were established using Monasurs anka KFCC 4478. The optimal temperature and initial pH for the production of red pigment were 30$^{\circ}C$ and 7.0, respectively. Glucose turned out to be most suitable carbon source for red pigment production. Optimal glucose concentration was 3.0%. Addition combined of nitrogen sources of peptone and NaNo3 induced good red pigment production. Thiamine-HCI and nicotinic acid were increased the production of red pigment. Under optimal conditions, maximum red pigment production and cell growth were observed after 5 days of incubation.

• 한국식품영양학회지
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• 제11권3호
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• pp.267-271
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• 1998

Seyferth 시약인 phenyl(tribromomethyl) mercury 및 diazomethane의 식품보존제인 dehydroacetic acid 및 coumarin과의 발암성과 그 반응기작을 분자 구조적 관점에서 알아보기 위하여 UV/vis 조건과 laser flash photolysis 시켰을 때의 결과는 다음과 같다. 1. Diazomethane과 Phenyl(tribromomethyl) mercury와 dehydroacetic acid와 diazomethane과의 반응은 광화학적 분해 반응에서 생성된 : CBr2와 : CH2 카르벤 중간체의 이탈기 효과에 따라 발암성이 크게 나타났다. 2. laser flash photolysis 상에서 반응시켰을 때 식품보존제의 발암성이 UV/vis 상에서의 photolysis 때보다 크게 나타났다.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.497-502
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• 2002

Thirty-nine strains of Leuconostocs found to be tolerant to $10\%$ or more garlic were selected for further identification, by comparing their whole-cell protein pattern, 16S rRNA gene (first 530 bases) sequence, cellular fatty acid composition, and carbon source metabolism. Two isolates were Identified as Leuconostoc mesenteroides and 32 others as Leuconostoc citreum. Five other strains belonging to a cluster could not be allocated to the existing species. 16S rRNA gene sequence and cellular fatty acid composition of the unidentified bacteria exhibited close similarity with Leuconostoc argentinum. The unidentified isolates were not allocated to L. argentinum, because they formed polysaccharide from sucrose, while L. argentinum strains do not. Leuconostocs tolerant to high concentration of garlic were found predominantly on garlic surface, an extreme environment which is unfit for most of other microorganisms.

• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1250-1260
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• 2010

Mycotoxins are secondary metabolites produced by fungi. These toxins pose serious health concerns to animals as well as human beings. Biodegradation of these mycotoxins has been considered as one of the best strategies to decontaminate food and feedstuffs. Biodegradation employs the application of microbes or enzymes to contaminated food and feedstuffs. Ruminants are considered to be resistant to the adverse effects of mycotoxins presumably due to the biodegrading ability of rumen microbes compared to mono-gastric animals. Therefore, rumen microbial source or microbial enzyme could be a great asset in biological detoxification of mycotoxins. Isolation and characterization of pure culture of rumen microorganisms or isolation and cloning of genes encoding mycotoxin-degrading potential would prove to have overall beneficial impact in the food and feed industry.