• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.69-72
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• 2005

In this study, effects of IHPs with various resting times to cell adhesion were investigated through the measurements of cell adhesive force, number and area of focal contacts (stained vinculin spots), and projected cell area, perimeter and circularity. In addition correlation tests and curve estimations using the experimental results were performed fur the finding an essential factor for increment of cell adhesive force. Tn the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). Average projected area and perimeter of cells at Group 5 were increased significantly (p<0.05), while average circularity of cells at Group 5 incubated fur 210 minutes was decreased significantly (p<0.05). In the digital image analysis of focal contacts containing vinculins, area and numbers of focal contacts per cell at Group 5 were higher than those of the other groups. This study indicated that IHP with appropriate resting time could contribute in improving cell adhesive force, cell spreading, development of cytoskeleton and formation of focal contacts. And cell adhesive force was correlated to the morphological aspects of cell and development of focal contacts. Particularly, area of focal contacts was closely related to cell adhesive force.

• Journal of Korean Neurosurgical Society
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• 제38권4호
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• pp.293-298
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• 2005

Objective : The authors investigate the spatial characteristics of apoptotic genes expressed around the focal cerebral infarction, and attempted to explain the penumbra with them. Methods : A delayed focal cerebral infarction was created in twelve adult Sprague-Dawley rats. We performed the immunohistochemical staining for the apoptosis, bcl-2 and p53 proteins and measured the local cerebral blood flow [CBF] at the infarction core area and peri-infarct area pre- and intra-operatively. The peri-infarct area was divided into six sectors by distance from the infarction border. Results : The size [$mm^2$] of apoptosis, bcl-2, and p53 areas were $3.1{\pm}1.2$, $4.7{\pm}2.1$, and $6.8{\pm}2.4$, respectively. Apoptosis, bcl-2 or p53 positive cells were concentrated at the peri-infarct area adjacent to the infarction core. Their numbers reduced peripherally, which was inversely proportional to the local CBF. The p53 area seems to overlap with and larger than the ischemic penumbra. Conclusion : The p53 positive area provides a substitutive method defining the penumbra under the molecular base of knowledge.

• 대한한의학회지
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• 제27권2호
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• pp.70-85
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• 2006

Objectives; This study examined the neuroprotective effect of Hyulbuchookautang (血府逐瘀湯, HBCAT)against neural damage following focal cerebral infarction. Methods : Sprague-Dawley Rats were induced with focal cerebral infarction by temporal middle cerebral artery occlusion (MCAO). The rats were divided into 2 groups. We treated extract of HBCAT to one group after operation (sample group), and the other group wasn't treated after operation (control group). We observed neurological scores and TIC-stained infarct area, total infarct volume in brain sections and Bax-positive neurons, HSP70- positive neurons in brain regions. Results : HBCAT treatment at 3 days after MCAO reduced neurological scores induced by MCAO. HBCAT treatment at 5 days after MCAO reduced TTC-stained infarct area in brain sections induced by MCAO. HBCAT treatment at 5 days after MCAO reduced total infarct volume in brain sections induced by MCAO. HBCAT treatment after MCAO reduced Bax-positive neurons in cortex infarct core and cortex infarct penumbra and caudo-putamen of brain regions induced by MCAO. HBCAT treatment after MCAO reduced HSP70- positive neurons in cortex infarct penumbra of brain regions induced by MCAO. Conclusions : These results suggest that HBCAT has a neuroprotective effect against focal cerebral ischemia.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제39권2호
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• pp.94-99
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• 2013

Focal osteoporotic bone marrow defect (FOBMD) is a radiolucent area corresponding to the presence of hematopoietic tissue rarely found in the jaws. FOBMD is most commonly located in the mandibular edentulous posterior area of a middle-aged female. From November 2011 to November 2012, we experienced three cases involving removal of implants that had accidentally fallen into the FOBMD area. All patients happened to be female, with a mean age of 54 years (range: 51-60 years). One case involved hypoesthesia of the lower lip and chin, while two cases healed without any complication. Displacement of an implant into the FOBMD area is an unusual event, which occurs rarely during placement of a dental fixture. The purpose of this study was to report on three cases of FOBMD and to provide a review of related literature.

• 한국방사선학회논문지
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• 제5권5호
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• pp.223-229
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• 2011

X선은 X선관 내 음극측 전자(electron)를 빠른 속도로 가속시킨 다음 진행하는 전자의 흐름을 저지극(target)에서 차단시킬 때 에너지의 변환을 일으켜 발생한다. 가속된 고속의 전자가 저지면에 충돌하는 실제면적을 실초점(actual focal spot)이라 하고, 실초점의 크기를 X선이 나오는 방향인 중심선(central ray)측에서 관측할 경우 축소되어 작게 보이는데 이때의 초점을 실효초점(effective focal spot)이라고 한다. X선관 방사각에 따라 음극 측의 강도가 양극 측 보다 높게 나타나 X선 강도가 균등하지 않다. 이러한 효과를 경사각 효과(heel effect)라고 하며, 경사각 효과로 인하여 환자가 받는 피폭의 정도는 양극의 각도, 즉 실효초점의 크기에 따라 달라지게 된다. 본 논문에서는 실효초점의 크기와 그에 따른 환자 피폭선량의 상관관계를 알아보고 실효초점의 크기에 따른 균질선량 분포를 위한 효과적인 조사야를 제시하고자 한다. 결론적으로 초점크기에 따라서 평균적으로 -8cm ~ 0cm 범위에서 효과적인 조사야 범위를 찾을 수 있었고, 평균 선량률은 0.019 R/min이 나왔다. 이 범위를 이용하면 환자에게는 적은 피폭선량으로 균등한 흑화도 및 해상력을 가진 영상을 얻을 수 있을 것이다.

• 한국광학회지
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• 제24권2호
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• pp.71-76
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• 2013

형광상관분광법을 이용하여 광세기에 따른 공초점 시스템의 유효 초점 부피의 변화를 분석하였다. 형광상관분광장치는 632.8 nm 파장의 He-Ne 레이저에 맞춰서 실험실에서 자체 제작하였고, 시료 또한 레이저 파장에 적합한 두 종류의 시료 AlexaFluor647과 quantum dot 655를 사용하였다. 각 시료에 대해 광원의 세기를 1~50 ${\mu}W$ 범위내에서 변화시켜가며 얻어진 상관함수를 비교 분석하였다. 10 ${\mu}W$ 이하의 약한 광 세기에서는 세기 변화에 따라 입자수와 확산시간이 증가하는 것을 통해 초점 영역의 반지름이 선형적으로 증가하는 결과를 보였다. 반면 10~15 ${\mu}W$ 이상에서는 입자의 수와 확산 시간의 증가율은 감소하였지만 미세하게 계속해서 증가하는 결과를 보였고, 이 결과를 통해 초점영역의 반지름 역시 증가율은 감소하였지만 미세하게 증가한 것을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6155-6160
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• 2014

Background: This study was conducted to investigate whether apparent diffusion coefficient (ADC) measurements by dividing the liver into left and right hepatic lobes may be utilized to improve the accuracy of differential diagnosis of benign and malignant focal liver lesions. Materials and Methods: A total of 269 consecutive patients with 429 focal liver lesions were examined by 3-T magnetic resonance imaging that included diffusion-weighted imaging. For 58 patients with focal liver lesions of the same etiology in left and right hepatic lobes, ADCs of normal liver parenchyma and focal liver lesions were calculated and compared using the paired t-test. For all 269 patients, ADC cutoffs for focal liver lesions and diagnostic accuracy in the left hepatic lobe, right hepatic lobe and whole liver were evaluated by receiver operating characteristic curve analysis. Results: For the group of 58 patients, mean ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. For differentiating malignant lesions from benign lesions in all patients, the sensitivity and specificity were 92.6% and 92.0% in the left hepatic lobe, 94.4% and 94.4% in the right hepatic lobe, and 90.4% and 94.7% in the whole liver, respectively. The area under the curve of the right hepatic lobe, but not the left hepatic lobe, was higher than that of the whole liver. Conclusions: ADCs of normal liver parenchyma and focal liver lesions in the left hepatic lobe were significantly higher than those in the right hepatic lobe. Optimal ADC cutoff for focal liver lesions in the right hepatic lobe, but not in the left hepatic lobe, had higher diagnostic accuracy compared with that in the whole liver.

• 한국지진공학회논문집
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• 제11권6호
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• pp.33-39
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• 2007

2004년 5월 29일 발생한 울진지진에 대해 지진원 상수(지진원기구, 진원깊이, 규모, 지진원 특성 등)를 모멘트텐서 방법을 이용하여 분석하였다. 3종류의 지각모델에 대해 지각응답함수를 구하여 분석에 이용하였다. 또한 최적의 지진원 상수값을 분석하기 위해 3종류의 진앙위치를 고려하여 분석하였다. 관측소의 방위각 분포 및 진앙거리에 대해서 결과값에 약간의 영향을 주었다. 6개의 모멘트텐서 성분을 조합하여 분석한 결과 울진지진은 거의 남북방향의 주향을 가진 전형적인 역단층의 운동에 의해 발생되었다. 분석된 지진원 기구는 울진지진 진앙 주변은 동서방향의 압축방향을 가진 지체역학적인 환경을 가지고 있는 것으로 제시하고 있다. 진원깊이는 약 12km의 값을 가지고 있다. 지진원기구는 기존의 연구결과와 유사하나 진원깊이는 다소 차이가 존재하였다. 이러한 차이는 방법론, 자료 종류 또는 지진원 고유의 기하학적 형태 등에 기인하는 것으로 해석된다.

• Korean Journal of Acupuncture
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• 제35권4호
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• pp.187-202
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• 2018

Objectives : Non-invasive transcranial electrical stimulation is one of therapeutic interventions to change in neural excitability of the cortex. Transcranial electro-acupuncture (TEA) can modulate brain functions through changes in cortical excitability as a model of non-invasive transcranial electrical stimulation. Some composites of fermented Scutellaria baicalenis (FSB) can activate intercellular signaling pathways for activation of brain-derived neurotrophic factor that is critical for formation of neural plasticity in stroke patients. This study was aimed at evaluation of combinatory treatment of TEA and FSB on behavior recovery and cortical neural excitability in rodent focal stroke model. Methods : Focal ischemic stroke was induced by photothrombotic injury to the motor cortex of adult rats. Application of TEA with 20 Hz and $200{\mu}A$ in combination with daily oral treatment of FBS was given to stroke animals for 3 weeks. Motor recovery was evaluated by rotating bean test and ladder working test. Electrical activity of cortical pyramidal neurons of stroke model was evaluated by using multi-channel extracellular recording technique and thallium autometallography. Results : Compared with control stroke group who did not receive any treatment, Combination of TEA and FSB treatment resulted in more rapid recovery of forelimb movement following focal stroke. This combination treatment also elicited increase in spontaneous firing rate of putative pyramidal neurons. Furthermore expression of metabolic marker for neural excitability was upregulated in peri-infract area under thallium autometallography. Conclusions : These results suggest that combination treatment of TEA and FSB can be a possible remedy for motor recovery in focal stroke.

• 대한한의학회지
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• 제25권1호
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• pp.117-128
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• 2004

Objectives : This research was performed to investigate effect of Samul-tang-gamibang against focal cerebral ischemic damage after middle cerebral artery occlusion(MCAO). Methods : This research was used rats which were against focal cerebral ischemic damage by MCAO. It was used Zea Longa's theory and Belayev's methods to give rise to focal cerebral ischemic damage by MCAO. After 7days later, we drew out the brain and then had frozen and dyeing it and we had taken a picture to measure of the damaged area in each brain section. We determined the Neurological Index and tested the Foot-fault test and Roatated test to appraise the fall of motion ability result from cerebral ischemic damage. Results : The results of the experiment are as follows. 1. Samul-tang-gamibang reduced infarct size of sample group compared to control group at 7 day after MCAO. 2. Samul-tang-gamibang reduced infarct volume of sample group compared to control group at 7 day after MCAO. 3. Samul-tang-gamibang reduced foot-fault index of sample group compared to control group at 5,7 day after MCAO. Conclusions : Samul-tang-gamibang has protective effects against ischemic brain damage and had significant reduced infarct size and infarct volume of Rt-MCAO.