• Bulletin of the Korean Chemical Society
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• 제28권9호
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• pp.1573-1578
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• 2007

Fluorescence quenching of norfloxacin (NOR) by Cu2+, Ni2+, Co2+ and Mn2+ was studied in water. The change in the fluorescence intensity and lifetime was measured as a function of quencher concentration at various temperatures. According to the Stern-Volmer plots, the NOR was quenched both by collisions and complex formation with the same quencher. However, the static quenching had a more important effect on the emission. Large static and dynamic quenching constants support significant ion-dipole and orbital-orbital interactions between NOR and cations. The both quenching constants by Cu2+ were the largest among quenchers. Also, quenching mechanism of Cu2+ was somewhat different. The change in the absorption spectra due to the quencher provided information on static quenching. The fluorescence of NOR was relatively insensitive to both the dynamic and static quenching compared with other quinolone antibiotics. This property can be explained by the twisted intramolecular charge transfer.

• 설비공학논문집
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• 제25권12호
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• pp.668-673
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• 2013

In this study, specimens with nano-sized porous thin films were manufactured by injecting fluorescence solution into the pores. We intended to find out the difference of the fluorescence intensity in each region of the specimen through an experimental apparatus that makes a temperature field. Before conducting experiments, the optimized manufacturing conditions were determined by analysis of all parameters that influence the emission intensity, and the experiments were carried out with the specimens produced in the optimized conditions. Then, the calibration curves of the fluorescence intensity versus temperature were performed by taking the intensity distributions from the specimen in various temperature fields. The surfaces of specimens were coated with Rhodamine-B (Rh-B) fluorescent dye and measured based on the fluorescence intensity. Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescence dye was absorbed into these porous thin films.

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1031-1034
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• 2009

Fluorescence of quinolones including norfloxacin, ciprofloxacin and S- and R-ofloxacin is quenched upon association with single and double-stranded DNA (ss- and ds-DNA). The ratios of fluorescence intensity in the presence of DNA to its absent were plotted with respect to the DNA concentration to construct the Stern-Volmer plot. The slope of the Stern-Volmer plot become larger as the temperature is lowered, ensuring that the fluorescence quenching is static process, i.e., the fluorescence is quenched by formation of the non-fluorescent complex between quinolone and DNA. In the static quenching mechanism, the quenching constant which is equivalent to the slope of the Stern-Volmer plot, is considered as the equilibrium constant for the association of quinolones and DNA. From the temperature-dependent equilibrium constant, ${\Delta}H^0\;and\;{\Delta}S^0$ was obtained using the van’t Hoff relation. In general, association of the quinolone with ds- as well as ss-DNA is energetically favorable (an exothermic) process while the entropy change was unfavorable. Due to the steric effect of the substituents, the effect of the quinolone ring is smaller on the ss-DNA compared to ds-DNA.

• 대한기계학회논문집B
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• 제31권2호
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• pp.159-166
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• 2007

Mixing between two or more reagents is one of important processes in biochemical microfluidics. In efficient micromixer design, it is essential to analyze flow pattern and evaluate mixing efficiency with good precision. In this work, mixing efficiency for Y-channel micromixer is measured by fluorescence intensity using LIF(Laser Induced Fluorescence) Confocal Microscope. The Y-channel micromixers are fabricated with polydimethylsiloxane(PDMS) and those are bonded to glass plate through Plasma bonding. Nile Blue A is injected into the micromixer as a fluorescence dye for measuring of fluorescence intensity by He/Ne laser. For visualization of the flow pattern, dynamic image capturing is carried out using CAM scope. For the comparison with computer simulation, modified SIMPLE algorithm for incompressible flow equation is solved for the same geometry as in the experiment. Throughout the experiments and computer simulation, accurate mixing efficiency evaluation process for a PDMS Y-channel micromixer is established.

• Bulletin of the Korean Chemical Society
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• 제34권5호
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• pp.1378-1382
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• 2013

We have investigated the complex forming behavior of cucurbit[6]urils(CB6) and cucurbit[7]urils(CB7) with 7-diethylamino-4-methylcoumarin(C460) in water. The electronic absorption maximum of C460 shows bathochromic shift with the addition of CB7 and fluorescence intensity is greatly increased, while CB6 has no noticeable effects on the spectroscopic properties of C460. It is noted that CB7 interacts more strongly with C460 than CB6 does. Fluorescence lifetime also significantly increased for the CB7 complex, which is attributed to reduced polarity surrounding C460 and/or C460 being in a restricted environment. The stoichiometry for the complex formation determined from the fluorescence titration measurement indicates that 2:1 complex in which two CB7 molecules bind to C460 is formed. Thus, two step equilibrium processes are suggested for the complex formation and the binding constants are estimated. The semi-empirical electronic structures calculations indicate that C460 is not included in the CB7 cavity but interacts noncovalently with the portal carbonyls of CB7.

• The Korean Journal of Ceramics
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• 제6권4호
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• pp.359-363
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• 2000

Reduced nicotinamide adenine dinucleotide (NADH) was encapsulated in transparent porous sol-gel silicate gels using by different organoalkoxysilane precursor. Characteristics optical absorption and fluorescence of NADH in the gels were examined with depending on NADH concentration and compared. Optical absorption in the aminopropyltrimethoxysilane (APTMS) gel is highest and remains constant during aging the gel. Thus, it is found that NADH in the APTMS gel is most stable and activated. On the other hand, methyltriethoxysilane (MTES) gel presents the lowest optical absorption diminishing with aging the gel. Measurable increase of fluorescence with raising the NADH concentration is observed except for the APTMS gel due to its solubility in the buffer during fluorescence measurement.

• 한국자동차공학회논문집
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• 제12권1호
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• pp.74-82
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• 2004

Planar laser-induced fluorescence(PLIF) has been widely used to obtain two dimensional fuel distribution. Acetone PLIF is chosen because fluorescence signal from acetone as a fluorescent tracer is less sensitive to oxygen quenching than other dopants. Acetone PLIF is applied to measure quantitative air excess ratio distribution in an engine fueled with LPG. Acetone is excited by KrF excimer laser (248nm) and its fluorescence image is acquired by ICCD camera with a cut-off filter to suppress Mie scattering from the laser light. For the purpose of quantifying PLIF signal, an image processing method including the correction of laser sheet beam profile is suggested. Raw images are divided by each intensity of laser energy and profile of laser sheet beam. Inhomogeneous fluorescence images scaled with the reference data, which is taken by a calibration process, are converted to air excess ratio distribution. This investigation shows instantaneous quantitative measurement of planar air excess ratio distribution for gaseous fuel.

• 한국염색가공학회지
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• 제30권4호
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• pp.237-244
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• 2018

Three super hydrophobic red fluorescence dyes were selected to dye high molecular weight polyethylene fiber. Their absorbance and emission spectra were obtained and Stokes' shift was measured. Fluorescence emission strength of the dyes on the fiber was investigated and therefore Fluoro Red 3 was determined as the best one among those three dyes in this experiment. Dyeing properties and fluorescence intensities were investigated using the Fluoro Red 3 on high molecular weight polyethylene fiber at various dyeing conditions. The optimum concentration of a dispersing agent was appeared at 10wt% in aqueous solution. The best dyeing was obtained at $125^{\circ}C$ for 1 hour. The color fastnesses to the washing and rubbing were as high as ratings 4~5, however, the fastness to light was exhibited ratings 2~3.

• 반도체디스플레이기술학회지
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• 제18권1호
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• pp.105-111
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• 2019

The TADF (Thermally Activated Delayed Fluorescence)-based OLED patents were analyzed and 4410 of patents were selected at the first step. And 975 patents were screened at second step. Finally, 39 key patents were selected. Patent qualitative analysis was performed in these patents to find which of the four property (lifetime, efficiency, color purity, driving voltage) of TADF was improved. Also, the variation of the hosts and dopants in patented TADF material were surveyed and their combination was analyzed. According to the analysis of the variation and the combination, some of TADF compounds were used as an assistant dopant to transfer energy. In addition, it tended to transfer energy by forming exciplex that shows TADF characteristics. These were similar to the mechanism of the introduced hyper fluorescence and could solve the inherent TADF problems. Finally, patent citation network was illustrated to visualize the patent citations and citations relationship of the major applicants in the current TADF-based OLED technology. The leading patent applicant organization was revealed as Idemitsu Kosan, Semiconductor Energy Laboratory, UDC, Princeton University, Merck and Nippon Steel & Sumikin Chemical, which had lots of reference patents 559, 524, 477, 310, 258, and 167, respectively.

• Bulletin of the Korean Chemical Society
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• 제7권3호
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• pp.196-200
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• 1986

The solvent change and salt do not affect the fluorescence quantum yield of 1,3-dimethylnaphtho[1,2-e]uracil indicating the considerable energy gap between the lowest singlet $({\pi},\;{\pi}^{\ast})\;and\;(n,\;{\pi}^{\ast})$ states in the compound. The results are consistent with the strong quenching of fluorescence by ethyl iodide. Fluorescence quantum yield is nearly independent of temperature, probably due to the relatively inefficient internal conversion. Unusual spectral difference is observed in isopentane and ethanol at 77K. The temperature dependence of emission in isopentane and in ethanol suggests that the increase of charge transfer character by the conformational change in isopentane leads to the structureless and red-shifted fluorescence, while in ethanol the decrease of the charge transfer character by the hydrogen bonding interaction results in the structured and blue-shifted fluorescence along with phosphorescence at the low temperature. Temperature dependence of emission in poly(methylmethacrylate) matrix indicates that $T_1{\to}S_0$ radiationless decay is an important process responsible for the strong temperature dependence of phosphorescence.