DOI QR코드

DOI QR Code

Mixing Efficiency Evaluation in Y-channel Micromixer Using LIF Confocal Microscope

LIF 공초점 현미경을 이용한 Y-채널 마이크로믹서의 혼합 효율 평가

  • 김경목 (한양대학교 대학원 기계공학과) ;
  • 신용수 (한양대학교 대학원 기계공학과) ;
  • 안유민 (한양대학교 기계정보공학부) ;
  • 이도형 (한양대학교 기계정보공학부)
  • Published : 2007.02.01

Abstract

Mixing between two or more reagents is one of important processes in biochemical microfluidics. In efficient micromixer design, it is essential to analyze flow pattern and evaluate mixing efficiency with good precision. In this work, mixing efficiency for Y-channel micromixer is measured by fluorescence intensity using LIF(Laser Induced Fluorescence) Confocal Microscope. The Y-channel micromixers are fabricated with polydimethylsiloxane(PDMS) and those are bonded to glass plate through Plasma bonding. Nile Blue A is injected into the micromixer as a fluorescence dye for measuring of fluorescence intensity by He/Ne laser. For visualization of the flow pattern, dynamic image capturing is carried out using CAM scope. For the comparison with computer simulation, modified SIMPLE algorithm for incompressible flow equation is solved for the same geometry as in the experiment. Throughout the experiments and computer simulation, accurate mixing efficiency evaluation process for a PDMS Y-channel micromixer is established.

Keywords

References

  1. Stroock, A. D., Dertinger, S. K. W., Ajdari, A., Mezic, I., Stone, H. A. and Whitesides, G. M., 2002, 'Chaotic Mixer for Microchannel,' Science, Vol. 295, pp. 647-651 https://doi.org/10.1126/science.1066238
  2. Nguyen, N. T. and Wereley, S. T., 2002, 'Fundamentals and Applications of Microfluidics,' Artech House, Boston, pp. 386-401
  3. Yang, Z., Goto, H., Matsumoto, M. and Maeda, R., 2002, 'Active Micromixer for Microfluidic Systems Using Lead-Zirconate-Titanate(PZT)-Generated Ultrasonic Vibration,' Electrophoresis, Vol. 21, Issue 1, pp. 116-119 https://doi.org/10.1002/(SICI)1522-2683(20000101)21:1<116::AID-ELPS116>3.0.CO;2-Y
  4. Knight, J. B., Vishwanath, A., Brody, J. P. and Austin, R. H., 1998, 'Hydrodynamic Focusing on a Silicon Chip: Mixing Nanoliters in Microseconds,' Phys. Rev. Lett., Vol. 80, No. 17, pp. 3863-3866 https://doi.org/10.1103/PhysRevLett.80.3863
  5. Wolfgang, E., Volker, H. and Holger, L., 2002, 'Microreactors, ' Wiley, Newyork, pp. 41-85
  6. Schwesinger, N., Frank, T. and Wurmus, H., 1996, 'A Modular Microfluid System with an integrated Micromixer,' J. Micromech. Microeng., Vol. 6, pp. 99-102 https://doi.org/10.1088/0960-1317/6/1/023
  7. Koch, M., Chatelain, D., Evans, A. G. R. and Brunnschweiler, A., 1998, 'Two Simple Micromixers Based on Silicon,' J. Micromech. Microeng., Vol. 8, pp. 123-126 https://doi.org/10.1088/0960-1317/8/2/020
  8. Wang, H., Iovenitti, P., Harvey, E. and Masood, S., 2002, 'Optimizing Layout of Obstacles for Enhanced Mixing in Microchannels,' Smart Materials and Structures, Vol. 11, pp. 662-667 https://doi.org/10.1088/0964-1726/11/5/30610.1088/0964-1726/11/5/306
  9. Choi, J. W., Choi, H. I., Lee, D. H. and Lee, D., 2005, 'Study on Mixing Enhancement of a Y-channel Micromixer with Obstacles,' Trans. of the KSME(B), Vol. 29, No. 12, pp. 1369-1376
  10. Shin, Y. S., Choi, H. I., Lee, D. H. and Lee, D., 2006, 'Optimum Design of a Y-channel Micromixer for Enhanced Mixing,' Trans. of the KSME(A), Vol. 30, No. 3, pp. 302-309 https://doi.org/10.3795/KSME-A.2006.30.3.302
  11. Ryu, K. S. and Liu, C., 2002, 'Precision Patterning of PDMS Thin Films : A New Fabrication Method and Its Applications,' Micro Total Analysis Systems, Vol. 1, pp. 112-114
  12. Yoon, S. Y. and Kim, K. C., 2006, 'Signal Intensity Enhancement of ${\mu}-LIF$ by Using Ultra-thin Laser Sheet Illumination and Aqueous Mixture with Ethanol/Methanol for Micro-channel Application,' Optics and Lasers in Eng., Vol. 44, pp. 224-239 https://doi.org/10.1016/j.optlaseng.2005.04.001
  13. Wong, S. H., Ward, M. C. L. and Wharton, C. W., 2004, 'Micro T-mixer as a Rapid Mixing Micromixer,' Sensors and Actuators B, Vol. 100, pp. 359-379 https://doi.org/10.1016/j.snb.2004.02.008
  14. Jeremy, E. M., Camille A. Boulet, and Charles A. Lucy., 2001, 'Indirect Laser-Induced Fluorescence Detection for Capillary Electrophoresis Using a Violet Diode Laser,' Anal, Chem, Vol. 73, pp. 1809-1813 https://doi.org/10.1021/ac001301u
  15. Julius, C. F., Stephen C. Jacobson and J. Michael Ramsey., 1999, 'Ultrasensitive Cross-Correlation Electrophoresis on Microchip Devices,' Anal, Chem, Vol. 71, pp. 4460-4464 https://doi.org/10.1021/ac990853d
  16. Jeon, N. L., Dertinger, S. K. W., Chiu, D. T., Choi, I. S., Stroock, A. D. and Whitesides, G. M., 2000, 'Generation of Solution and Surface Gradients Using Microfluidic Systems,' Langmuir, Vol. 16, pp. 8311-8316 https://doi.org/10.1021/la000600b
  17. Norbert, k., Thomas, k. and peter, w., 2004, 'Numerical and Experimental Investigation on Liquid Mixing in Static Micromixers,' Chemical Engineering Journal, Vol. 101, pp. 315-322 https://doi.org/10.1016/j.cej.2003.10.017