• 제목/요약/키워드: Fluorescence dye

검색결과 214건 처리시간 0.306초

NO PLIF용 excitation 레이저에 관한 연구 (The Study on the Excitation Lasers for NO Planar Laser-Induced Fluorescence Imaging)

  • 강경태
    • 한국연소학회:학술대회논문집
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    • 한국연소학회 1997년도 제15회 KOSCO SYMPOSIUM 논문집
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    • pp.13-21
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    • 1997
  • Excitations of eight pumping transitions for nitric oxide fluorescence imaging are analyzed under equivalent experimental conditions to determine the detection. Frequency mixed dye laser pumping, 1st anti-Stokes $H_2$ Raman of KrF excimer laser pumping and ArF excimer laser pumping show good sensitivities.

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Fluorescence Micropatterning Based on the Polymeric Photobase Generator Containing Oxime-Urethane Groups by Dansylation

  • Choi, Won San;Kim, Hak Soo;Chae, Kyu Ho
    • Rapid Communication in Photoscience
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    • 제2권1호
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    • pp.24-27
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    • 2013
  • A polymeric photobase generator containing oxime-urethane groups is applied to a fluorescence micropatterning material. Polymer bearing oxime-urethane groups was prepared by copolymerization of methyl methacrylate with methacryloyloxyethyl benzophenoneoxime urethane (MBU). The reaction of amino groups in the irradiated copolymer film with dansyl chloride (Dns-Cl) was monitored by using UV absorption, IR absorption and fluorescence spectroscopy. The fluorescence spectrum of the Dns-Cl-treated irradiated copolymer film shows a strong fluorescence with a fluorescence maximum wavelength at 510 nm. A blue fluorescent micropattern with a line width of $2{\sim}3{\mu}m$ was obtained. Treatment of the irradiated copolymer film with Dns-Cl and rhodamine B mixture led to the formation of green, red, and orange-colored fluorescence micropatterns. Thus, various colored micropatterns on a single polymer film can be obtained by selective excitation of each dye molecules.

형광 나노 포러스 박막을 이용한 표면 온도 센서의 제작 및 성능 연구 II (Fabrication and Performance Investigation of Surface Temperature Sensor Using Fluorescent Nanoporous Thin Film II)

  • 김현정;유재석;박진일
    • 설비공학논문집
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    • 제25권12호
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    • pp.674-678
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    • 2013
  • We present a non-invasive technique to the measure temperature distribution in nano-sized porous thin films by means of the two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence induced by the green line of a mercury lamp with the makeup of optical filters was measured on two separate color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows for absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. To measure temperature fields, Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescent dye was absorbed into these porous thin films. The calibration curves of the fluorescence intensity versus temperature were measured in a temperature range of $10-60^{\circ}C$, and visualization and measurement of the temperature field were performed by taking the intensity distributions from the specimen for the temperature field.

형광분광분석법에 의한 Methylene Blue와 Thionine의 Metachromasy (Metachromasy of Metylene Blue and Thionine by Analytical Fluorescence Spectroscopy)

  • 이후설
    • 한국응용과학기술학회지
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    • 제14권2호
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    • pp.103-114
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    • 1997
  • Metachromatic properties of admixture of methylene blue(MB) and thionine in aqueous solution has been studied by fluorescence spectroscopy. In spite of nonfluorescence character has been MB itself, mixing MB to monomeric concentration of thionine, new coaggregation band has been formed in shorter wave length than fluorescence of thionine because of MB was redistributed to thionine aggregate. It suggested that coaggregate of MB and thionine were more tightly formed than the each dye aggregate.

상호혼합에 의한 메로시아닌 색소 단분자막의 2차원 거동 및 J-회합체 형성 (Two-Dimensional Behavior and J-Aggregate Formation of Merocyanine Dye Monolayers in Mutual Mixing)

  • 신훈규
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제51권3호
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    • pp.105-110
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    • 2002
  • J-aggregates in the mutual mixing LB films of [6Me-DS]$_{1-x}$ [DO]$_{x}$,[DS]$_{1-x}$ [DO]$_{x}$ and [DSe]$_{1-x}$ [DO]$_{x}$ have been studied by optical absorption, fluorescence and surface pressure-area isotherms. In [6Me-DS]$_{1-x}$ [DO]$_{x}$ films, sharp J-band absorption and fluorescence of [6Me-DS] are linearly shifted to the longer wavelength for the replacement of [6Me-DS] by [DO]. According to the x, a smooth shift of the limited area has been cleared. In the [DS]-[DO] system, the J-band is enhanced at 1:1 composition and strong fluorescence is also observed. Also, the presence of phase separation was suggested in the [DSe]-[DO] system, because the absorption spectra were decomposed into [DSe] and [DO] spectra. On the other hand, in the pressure-area isothermal study, reduction in the molecular occupying area of monolayers has been clarified. This could be ascribed to the enhancement of molecular ordering in J-aggregates. These facts are also believed to reflect the most closely packed arrangement of chromophores in the merocyanine dye monolayers. Thus, it was confirmed that the interaction between mixed dye molecules and the CdC1$_2$+KHCO$_3$subphases affected the J-aggregates of the LB films. Also. it is thought that the J-aggregates are formed non-dimensionally in LB films, such as solution synthesized [DX:DO] assemblies on mixing.s on mixing.

Miniaturized Fluorometer Based on Total Internal Reflector and Condensing Mirror

  • Jang, Dae-Ho;Yoo, Jae-Chern
    • Journal of the Optical Society of Korea
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    • 제17권1호
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    • pp.81-85
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    • 2013
  • A miniaturized fluorescence detection system based on total internal reflection (TIR) configuration, which is applicable to detecting the presence of biological materials labeled with fluorescence dye in micro total analysis systems (${\mu}TAS$), is proposed. In conventional fluorescence testing and analysis devices, interference between the excitation light beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection system based on TIR configuration that allows the excitation light beam and the emitted light to be spatially perpendicular to each other so as to minimize the interference where fluorescence emission is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being comparable to that in earlier studies.

비파괴 자외-가시 및 형광 분광 분석법을 이용한 적색계 전통 염료 분석 (Analysis on Red-colored Dyeing by using Non-destructive UV-visible and Fluorescence Spectrophotometry)

  • 윤은영;김유란
    • 박물관보존과학
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    • 제12권
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    • pp.9-17
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    • 2011
  • 본고는 적색계 염재인 소목, 꼭두서니, 홍화, 자초로 염색한 직물편에 대한 비파괴 자외 가시광 분광분석 및 3차원 형광 분광 분석 내용으로 고대 직물에 염색된 염료 규명을 위한 기초 자료 구축을 위함이다. 직물이나 매염제의 종류에 따라 각 분석 결과에 영향을 주는지 여부를 확인하기 위하여 직물 2 종류(면과 견)와 매염 방법 3 가지(무매염, 백반, 철)로 염색한 직물시편을 제작하였다. 염색 직물편에 대한 자외-가시 분광반사 스펙트럼 결과 소목, 꼭두서니, 자초로 염색한 경우 직물의 종류에 관계없이 매염제에 따른 차이를 보였다. 또한 홍화는 직물 및 매염제에 따른 차이가 없었다. 3차원 형광 스펙트럼 측정결과 소목은 매염제에 따른 차이를 보이며 꼭두서니의 경우 직물에 따른 차이를 보였고, 홍화의 경우 직물과 매염제 관계없이 고유한 형광스펙트럼으로 나타났으며, 자초의 경우 형광스펙트럼이 나타나지 않았다.

초기 우식 병소에서 광원에 따른 형광효과 비교 (A COMPARISON OF FLUORESCENCE EFFECT OF VARIOUS LIGHT SOURCES IN EARLY ENAMEL CARIES)

  • 전정훈;이난영;이창섭;이상호
    • 대한소아치과학회지
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    • 제32권4호
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    • pp.611-619
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    • 2005
  • 본 연구의 목적은 초기 우식 병소에서 광원에 따른 형광효과를 비교하는 것으로 정량 분석형 laser/light 형광법(Quantitative laser/light-induced fluorescence, QLF)을 이용하여 아르곤 레이저광, 할로겐광, 발광다이오드광(LED), 플라즈마광의 형광효과를 비교하였다. 발거된 60개의 치아를 선정한 후, 인공우식용액에 노출되지 않을 부분에 nail varnish를 도포하였고 치아는 24, 48, 72시간 동안 인공우식용액에 보관되었다. 건조 후 QLF영상으로 초기 우식 부위의 광밀도 차이에 의한 탈회정도가 기록되었다. 또한 조영제를 이용하여 동일한 방법으로 실험하였고 다음과 같은 결과를 얻었다 염료를 이용하여 같은 방법으로 실험하여 또 다른 결과를 얻었다. 1. 각 군의 평균 광밀도를 볼 때, 플라즈마광이 세 군 모두에서 다른 광원들 보다 높았다(p<0.05). 2. 세 군 사이에서 평균 광밀도를 비교해 볼 때, 플라즈마광과 할로겐광이 차이를 보였다(p<0.05). 3. 조영제를 사용하였을 경우 평균 광밀도를 비교해 볼 때, 플라즈마광이 세 군 모두에서 다른 광원들보다 높았고 (p<0.05), 발광다이오드광(LED)와 아르곤 레이저광을 제외한 모든 광원이 차이를 보였다(p<0.05). 4. 조영제를 사용하지 않은 경우와 사용한 경우 평균 광밀도를 비교해 볼 때, 사용한 경우에 모든 광원의 평균 광밀도 차이가 컸다.

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Diheteryl-substituted triphenylamine 화합물의 합성과 형광 특성 (Synthesis and Photoluminescent Property of Diheteryl-substituted Triphenylamine Compound)

  • 김병순;김성훈;손영아
    • 한국염색가공학회지
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    • 제19권6호
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    • pp.35-38
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    • 2007
  • FTriphenylamine dye compound having diheteryl moiety was synthesized and its photoluminescent property was investigated. Organic luminescent materials have received great attentions due to potential application subjects onto full color image displays. In this context, the dye (III) for light emitting materials was synthesized using 2-(4-amino-2-hydroxyphenyl)benzoxazole (I) and 4,4'-diformyltriphenylamine (II). It is well known that the amino groups of compound (I) react with carbonyl groups, especially an aldehyde, to afford azomethine linkages. The dye shows bulish-green fluorescence property, which is anticipated for the light-emitting material for display devices. In this context, our aim is to synthesize diheteryl-substituted triphenylamine fluorescent dye as an emitting material. The spectroscopic characteristics and the fluorescent properties of this dye molecule were examined and determined.

Ar 섬광관의 방전 특성과 BBQ의 형광 강도 (Characteristics of Ar flashlamp and Fluorescence of BBQ)

  • 임춘우;박성진;방형배;오철한
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1992년도 하계학술대회 논문집 B
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    • pp.958-960
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    • 1992
  • The fluorescence characteristics of near UV dye, BBQ pumped by Ar flashlamp have been studied for various electrical input energies applied to the flashlamp and argon fill pressures in the flash tube. The peak wavelength of fluorescence is about 380nm when the fluorescence intensity is at the peak value. The rise time of the fluorescence pulse is about 7 $\mu$sec and its FWHM is about 55$\mu$sec.

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