• 제목/요약/키워드: Fluorescence Imaging

검색결과 238건 처리시간 0.021초

Single C-Reactive Protein Molecule Detection on a Gold-Nanopatterned Chip Based on Total Internal Reflection Fluorescence

  • Heo, Yunmi;Lee, Seungah;Lee, Sang-Won;Kang, Seong Ho
    • Bulletin of the Korean Chemical Society
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    • 제34권9호
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    • pp.2725-2730
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    • 2013
  • Single C-reactive protein (CRP) molecules, which are non-specific acute phase markers and products of the innate immune system, were quantitatively detected on a gold-nanopatterned biochip using evanescent field-enhanced fluorescence imaging. The $4{\times}5$ gold-nanopatterned biochip (spot diameter of 500 nm) was fabricated by electron beam nanolithography. Unlabeled CRP molecules in human serum were identified with single-molecule sandwich immunoassay by detecting secondary fluorescence generated by total internal reflection fluorescence (TIRF) microscopy. With decreased standard CRP concentrations, relative fluorescence intensities reduced in the range of 33.3 zM-800 pM. To enhance fluorescence intensities in TIRF images, the distance between biochip surface and CRP molecules was optimally adjusted by considering the quenching effect of gold and the evanescent field intensity. As a result, TIRF only detected one single-CRP molecule on the biochip the first time.

Sentinel lymph node mapping using tri-modal human serum albumin conjugated with visible dye, near infrared fluorescent dye and radioisotope

  • Kang, Se Hun;Kim, Seo-il;Jung, So-Youn;Lee, Seeyoun;Kim, Seok Won;Kim, Seok-ki
    • 대한방사성의약품학회지
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    • 제1권1호
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    • pp.62-73
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    • 2015
  • We developed an evans blue-indocyanine green-$^{99m}Tc$-human serum albumin conjugate for sentinel lymph node mapping and we describe its unique potential usage for clinical implications. This conjugate has combined the strengths of visible blue dye, near-infrared fluorescence and radioisotope into one single conjugate without any additional weakness/disadvantage. All the components of evans blue-indocyanine green-$^{99m}Tc$-human serum albumin are safe and of low cost, and they have already been clinically used. This conjugate was stable in the serum, it showed a long retention time in the lymphatic system and the lymph nodes showed a much higher signal-to-noise ratio after the conjugate was injected intradermally into the paw of mice. Both the single-photon emission computed tomography and near-infrared fluorescent images of the mice were successfully obtained at the same time as the excised sentinel lymph nodes showed blue color. The visual color, near-infrared fluorescence and gamma ray from this agent could be complementary for each other in all the steps of sentinel lymph node sampling: exploring and planning sentinel lymph node before excision with visualization of the exact sentinel lymph node location during an operation. Therefore, the triple modal agent will possibly be very ideal for sentinel lymph node mapping because of the high signal-to-noise ratio for non-invasive imaging and its complementary multimodal nature, easy preparation and safety. It is promising for clinical applications and it may have great advantages over the traditional single modal methods.

다기능 NSOM (mf-NSOM) 을 이용한 나노 구조 재료 분석에 관한 원리와 응용 (Fundamentals and Applications of Multi-functional NSOM Technology to Characterization of Nano Structured Materials)

  • 이우진;변수일
    • 전기화학회지
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    • 제7권2호
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    • pp.108-123
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    • 2004
  • 최근 근접장 광학주사현미경 (NSOM)을 이용한 재료의 표면 및 구조 분석은 생물학에서 재료과학에까지 광범위하게 응용되고 있다. 본 총설에서는 기존의 NSOM을 여러가지 현미경법 (광학, 형광, 전자 및 전기화학 현미경 관찰법)과 접목하여 구성한 다기능 NSOM (multi-functional NSOM, mf-NSOM)을 이용, 나노 재료의 고분해능 이미징에 대한 원리와 응용을 고찰하였다. 본 mf-NSOM 기술을 이용하여 실제로 Al합금 및 다결정 Ti 표면에서의 공식 (pitting)을 일으키는 취약 지역을 광학적으로 분석한 결과를 기술하였다. 또한, mf-NSOM과 레이저 기술을 통해 나노 Ag 입자를 형성하고 실시간 분석한 연구결과에 대해서도 소개하고자 한다.

Endoscopic Fluorescence Angiography with Indocyanine Green : A Preclinical Study in the Swine

  • Cho, Won-Sang;Kim, Jeong Eun;Kim, Sae Hoon;Kim, Hee Chan;Kang, Uk;Lee, Dae-Sic
    • Journal of Korean Neurosurgical Society
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    • 제58권6호
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    • pp.513-517
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    • 2015
  • Objective : Microscopic indocyanine green (ICG) angiography is useful for identifying the completeness of aneurysm clipping and the preservation of parent arteries and small perforators. Neuroendoscopy is helpful for visualizing structures beyond the straight line of the microscopic view. We evaluated our prototype of endoscopic ICG fluorescence angiography in swine, which we developed in order to combine the merits of microscopic ICG angiography and endoscopy. Methods : Our endoscopic ICG system consists of a camera, a light source, a display and software. This system can simultaneously display real-time visible and near infrared fluorescence imaging on the same monitor. A commercially available endoscope was used, which was 4 mm in diameter and had an angle of $30^{\circ}$. A male crossbred swine was used. Results : Under general anesthesia, a small craniotomy was performed and the brain surface of the swine was exposed. ICG was injected via the ear vein with a bolus dose of 0.3 mg/kg. Visible and ICG fluorescence images of cortical vessels were simultaneously observed on the display monitor at high resolution. The real-time merging of the visible and fluorescent images corresponded well. Conclusion : Simultaneous visible color and ICG fluorescent imaging of the cortical vessels in the swine brain was satisfactory. Technical improvement and clinical implication are expected.

A Quinoline-thiooxorhodamine Conjugate for Fluorescent Hg2+ Recognition in Aqueous Media and Living Cells

  • Tang, Lijun;Wen, Xin;Dai, Xin;Wu, Di;Huang, Zhenlong
    • Bulletin of the Korean Chemical Society
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    • 제35권11호
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    • pp.3326-3330
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    • 2014
  • A quinoline-thiooxorhodamine conjugate fluorescent sensor (1) has been synthesized. Sensor 1 exhibits high selectivity and sensitivity to $Hg^{2+}$ in $H_2O$/DMSO (95/5, v/v, HEPES 20 mM, pH = 7.4) solution with fluorescence detection. Other tested metal ions do not induce any significant fluorescence intensity changes. Sensor 1 interacts with $Hg^{2+}$ through a 1:1 binding stoichiometry with a good anti-inference ability. In addition, fluorescent imaging of $Hg^{2+}$ in Hela cells is also successfully demonstrated.

A comparison of photosynthesis measurements by O2 evolution, 14C assimilation, and variable chlorophyll fluorescence during light acclimatization of the diatom Coscinodiscus granii

  • Trampe, Erik;Hansen, Per Juel;Kuhl, Michael
    • ALGAE
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    • 제30권2호
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    • pp.103-119
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    • 2015
  • Photosynthetic rates of the large centric diatom Coscinodiscus granii were measured by means of multicolor variable chlorophyll fluorescence imaging, single cell $^{14}C$ assimilation, and optical $O_2$ sensor measurements during light acclimatization of cultures grown at five different irradiances: 50, 150, 235, 332, and $450{\mu}mol$ photons $m^{-2}\;s^{-1}$. Photo-acclimatization was evident from changes of cellular chlorophyll a content, growth rates, and light response curves. Each of the applied methods evaluates different parts and reactions in the photosynthetic apparatus, which makes a direct quantitative comparison of rates difficult, although a different degree of correlation were found between all three methods. However, when used in combination, they provide information about the internal relationship of photosynthetic pathways as well as the variation in photosynthetic capacity between individual cells within a single algal culture.

형광 영상 기반 CD4 신속 검사법 개발을 위한 항원-항체 반응 조건 정량화 (Quantitation of Antigen-Antibody Reaction Condition for Development of Fluorescence Image-based CD4 Rapid Test)

  • 김수빈;김중경
    • 한국가시화정보학회지
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    • 제13권1호
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    • pp.35-42
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    • 2015
  • CD4+ T-cell count determines the effectiveness for antiretroviral therapy (ART) in patients with human immunodeficiency virus (HIV). Although ART slows the progression of HIV to AIDS, rapid counting of CD4+ T lymphocytes with a drop of patient's blood sample is urgently needed to ensure timely ART treatment in rural areas. Recently point-of-care CD4 testing devices have been developed by using non-flow based imaging cytometer incorporated with a sample cartridge where CD4+ T cells are reacted with fluorescently tagged specific antibodies. Here we conducted an experimental study using a conventional fluorescence microscope-based imaging system to quantitate the interaction of CD4 antibodies with CD4+ T cells at different reaction conditions. We demonstrated that a fast and affordable point-of-care CD4 test is feasible with a far less amount of antibodies and a shorter incubation time compared with a conventional sample preparation protocol for flow cytometry. We also proposed a general method to evaluate and compare the detection limit across different CD4 counting platforms by using fluorescently labelled microbeads for intensity calibration.

Albumin-conjugated Cadmium Sulfide Nanoparticles and their Interaction with KB Cells

  • Selim, K.M. Kamruzzaman;Kang, Inn-Kyu;Guo, Haiqing
    • Macromolecular Research
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    • 제17권6호
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    • pp.403-410
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    • 2009
  • Cytotoxicity is a severe problem of cadmium sulfide nanoparticles(CSNPs) for use in biological systems. In the present study, mercaptoacetic acid-coated CSNPs were conjugated with bovine serum albumin (BSA) to improve biocompatibility. The surface properties of the CSNPs and albumin-conjugated CSNPs (ACSNPs) were characterized by XRD, UV, FTIR, EA, TEM and DLS. Human breast cancer cells (KB cells) were then cultured in the presence of the nanoparticles to evaluate the cytotoxicity of CSNPs and ACSNPs. Finally, the fluorescence intensity of the nanoparticles' aqueous solution was examined using a fluorescence spectrometer. The results showed that the cell compatibility and fluorescence intensity of ACSNPs were higher than those of CSNPs. The strongly luminescent features of the biocompatible ACSNPs are promising for use in biological fields such as cellular labeling, intracellular tracking and molecular imaging.

Fluorescence Image-Based Evaluation of Gastric Tube Perfusion during Esophagogastrostomy

  • Quan, Yu Hua;Han, Kook Nam;Kim, Hyun Koo
    • Journal of Chest Surgery
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    • 제53권4호
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    • pp.178-183
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    • 2020
  • During esophagectomy and esophagogastrostomy, the prediction of anastomotic leakage relies on the operating surgeon's tactile or visual diagnosis. Therefore, anastomotic leaks are relatively unpredictable, and new intraoperative evaluation methods or tools are essential. A fluorescence imaging system enables visualization over a wide region of interest, and provides intuitive information on perfusion intraoperatively. Surgeons can choose the best anastomotic site of the gastric tube based on fluorescence images in real time during surgery. This technology provides better surgical outcomes when used with an optimal injection dose and timing of indocyanine green.

MicroSPECT and MicroPET Imaging of Small Animals for Drug Development

  • Jang, Beom-Su
    • Toxicological Research
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    • 제29권1호
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    • pp.1-6
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    • 2013
  • The process of drug discovery and development requires substantial resources and time. The drug industry has tried to reduce costs by conducting appropriate animal studies together with molecular biological and genetic analyses. Basic science research has been limited to in vitro studies of cellular processes and ex vivo tissue examination using suitable animal models of disease. However, in the past two decades new technologies have been developed that permit the imaging of live animals using radiotracer emission, X-rays, magnetic resonance signals, fluorescence, and bioluminescence. The main objective of this review is to provide an overview of small animal molecular imaging, with a focus on nuclear imaging (single photon emission computed tomography and positron emission tomography). These technologies permit visualization of toxicodynamics as well as toxicity to specific organs by directly monitoring drug accumulation and assessing physiological and/or molecular alterations. Nuclear imaging technology has great potential for improving the efficiency of the drug development process.