• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.9-14
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• 2008

Efficient gene transfer into hematopoietic stem cells is a great tool for gene therapy of hematopoietic disease. Retrovirus have been extensively used for gene delivery and gene therapy. However, current in vitro gene transfer has some obstacles suck as induction of differentiation loss of self-renewal capacity, and down-regulation of homing efficiency for in vitro hematopoietic stem cells transplantation. To overcome these problems, we developed efficient in vitro retroviral transfer technique by direct intra-bone marrow injection (IBM). We identified effective retrovirus gene transfer in bone marrow hematopoietic cells in vitro. Two weeks after retrovirus transfer via IBM injection, we observed stable EGFP gene expression in bone marrow, lymph node, spleen, and liver cells. In addition, $6.4{\pm}2.7%$ of hematopoietic stem/progenitor cells were expressed EGFP transgene from flow cytometry analysis. Our results demonstrate that in vitro retrovirus gene transfer via IBM injection can provide a viable alternative to current or moo gene transfer approach.

• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.2
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• pp.112-121
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• 2007

In occasion of soil loss happened in a basin, soil in the near of a stream may flow into the stream easily, but in case that soil is far away from the stream, sediment yield transferred to rivers by rainfall diminishes. To forecast sediment yield of a stream is an essential item for management of basins and streams. Therefore, sediment yield of soil loss produced from a basin is needed to be calculated as accurate as possible. Purpose of the present research is to calculate soil erosion amount in a basin and to forecast sediment yield flowed into a stream by rainfall and analyze sediment yield in the stream. There are various methods that analyze sediment yield of rivers. In the present study, the soil erosion amount was calculated using Revised Universal Soil Loss Equation(RUSLE) and GRID, and sediment yield was calculated using sediment delivery ratio and empirical methods. DEM data, slope of basin, soil map and landuse constructed by GIS were used for input data of RUSLE. The upstream area of the Yeongsan river basin in Gwangju metropolitan city was selected for the study area. Three methods according to the calculation of LS factor were applied to estimate the soil erosion amount. Two sediment delivery ratio methods for the respective methods were applied and, correspondingly, six occasions in sediment yield were calculated. In addition, the above results were compared by relative amount with estimation by the empirical method of Ministry of Construction & Transportation. Sediment yield calculated in the present study may be utilized for the plan, design and management of dams and channels, and evaluation of disaster impact.

• Journal of Environmental Science International
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• v.16 no.3
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• pp.333-345
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• 2007

As analyzed the estimated values of the daily delivery loads from thirteen major side streams such as Naesung-river, Keumho-river, Hwang-river, Nam-river during five years (from 1999 to 2003), the daily BOD inflow into the main stream of Nakdong river in 2003 shows the highest quantity as 31.1 ton and the daily BOD inflows in 1999, 2000, 2001, and 2002 are 26.7 ton, 22.5 ton, 21.0 ton, 25.8 ton, respectively. The daily TN in-flow into the main stream of Nakdong river in 2003 shows also the highest quantity as 64.9 ton and the daily TN inflows in 1999, 2000, 2001, and 2002 are 55.19 ton, 46.27 ton, 39.5 ton, 53.38 ton, respectively. The daily TP inflow into the main stream of Nakdong river in 2003 shows the highest quantity as 2.70 ton likewise and the daily TP inflows in 1999, 2000, 2001, and 2002 are 2.17 ton, 1.87 ton, 1.60 ton, 2.10 ton, respectively The rate of BOD loads from each side main stream into the main stream of Nakdong river shows that the BOD loads of Keumho-river are the highest as the values range from 32.8 % (2002) ${\sim}$ 35.1 % (2003) and the BOD loads of Nam-river, Naesung-river, Hwang-river are high in the order named. The rate of TN loads to the main stream is also similar to the trend of BOD loads. The contribution of the TN loads of Geumho-river to the contamination of the main stream is also the highest having a range from 27.0 % (2002) to 28.8 % (1999) among the main side streams and the TN loads of Naesung-river, Nam-river, and Heachun-river are high in the order named. The rate of TP loads is quite different from the trend of BOD and TN loads. The TP rate of Keumho-river, however, is still the highest as ranging 58.6 % (2002) to 61.7 % (2003) and the river has the biggest portion (over 50%) of the entire pollution to the main stream of Nakdong river.

• ETRI Journal
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• v.42 no.4
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• pp.491-504
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• 2020

We present AB9, a neural processor for inference acceleration. AB9 consists of a systolic tensor core (STC) neural network accelerator designed to accelerate artificial intelligence applications by exploiting the data reuse and parallelism characteristics inherent in neural networks while providing fast access to large on-chip memory. Complementing the hardware is an intuitive and user-friendly development environment that includes a simulator and an implementation flow that provides a high degree of programmability with a short development time. Along with a 40-TFLOP STC that includes 32k arithmetic units and over 36 MB of on-chip SRAM, our baseline implementation of AB9 consists of a 1-GHz quad-core setup with other various industry-standard peripheral intellectual properties. The acceleration performance and power efficiency were evaluated using YOLOv2, and the results show that AB9 has superior performance and power efficiency to that of a general-purpose graphics processing unit implementation. AB9 has been taped out in the TSMC 28-nm process with a chip size of 17 × 23 ㎟. Delivery is expected later this year.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.234-237
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• 2003

The Korean housing supply have been provided by the Pre-construction sales system. The Pre-construction sales system contributed to large housing supply. But it followed by the market anomaly. Along the housing market is changing to tile market for consumers, it requires new policy and regulations. This market changes and needs to modify the policy make a discussion about introducing the Post-construction sales system. it concerns to change the time to sale. This paper analyzes the present feasibility study and makes a tool to predict construction cashflow considering changed sales point. The sales timing leads to decide the amount of financial costs in the construction project and that cost affects to the feasibility. The accurate cashflow prediction is required for a successful apartment construction delivery.

• The KIPS Transactions:PartC
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• v.11C no.2
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• pp.245-252
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• 2004

Stream Control Transmission Protocol(SCTP) is a new connection-oriented, reliable delivery transport protocol operating on top of an unreliable connectionless packet service such as IP. It inherits many of the functions developed for TCP, including flow control and packet loss recovery functions. In addition, it also supports transport layer multihoming and multistreaming In this paper, we study the impact of multi-homing on the performance of SCTP. We first compare performance of single-homed SCTP. multi-homed SCTP, TCP Reno and TCP SACK. We, then describe potential flaw in the current SCTP retransmission policy, when SCTP host is multihomed. Our Results show that SCTP performs better than TCP Reno and TCP SACK due to several changes from TCP in its congestion control mechanism. In particular. multi-homed SCTP shows the best result among the compared schemes. Through experimentation for multi-homed SCTP, we found that the current SCTP retransmission policy nay deteriorate the perfomance when the retransmission path it worse than the original path. Therefore, the condition of retransmission path is a very important factor In SCTP performance and a proper mechanism would be required to measure the condition of the retransmission path.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.6
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• pp.2425-2430
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• 2015

Clinical resistance to chemotherapeutic agents is one of the major hindrances in the treatment of human cancers. EHZ2 is involved in drug resistance and is overexpressed in drug-resistant cancer cell lines. In this study, we investigated the effects of EHZ2 on cisplatin -resistance in A549/DDP and AGS/DDP cells. EHZ2 mRNA and protein were found to be significantly overexpressed in A549/DDP and AGS/DDP cells, compared to parental cells. EHZ2 siRNA successfully silenced EHZ2 mRNA and protein expression. Proliferation was inhibited and drug resistance to cisplatin was improved. Flow cytometry showed that silencing of EHZ2 arrested A549/DDP and AGS/DDP cells in the G0/G1 phase, increasing apoptosis, rh-123 fluorescence intensity and caspase-3/8 activities. Silencing of EHZ2 also significantly reduced the mRNA and protein expression levels of cyclin D1 and MDR1,while up-regulating p15, p21, p27 and miR-218 in A549/DPP cells. Furthermore, silencing of EHZ2 also significantly increased the expression level of tumor suppressor factor miR-218. We also found down-regulating EHZ2 expression increased methylation in A549/DDP and AGS/DDP cells. This study demonstrates that drug resistance can be effectively reversed in human cisplatin-resistant lung and gastric cancer cells through delivery of siRNAs targeting EHZ2.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2447-2451
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• 2014

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1098-1105
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• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8595-8598
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• 2016

Background: Decision making modalities for screening for many cancer conditions and different stages have become increasingly complex. Computer-based risk assessment systems facilitate scheduling and decision making and support the delivery of cancer screening services. The aim of this article was to survey electronic risk assessment system as an appropriate tool for the prevention of cancer. Materials and Methods: A qualitative design was used involving 21 face-to-face interviews. Interviewing involved asking questions and getting answers from exclusive managers of cancer screening. Of the participants 6 were female and 15 were male, and ages ranged from 32 to 78 years. The study was based on a grounded theory approach and the tool was a semi-structured interview. Results: Researchers studied 5 dimensions, comprising electronic guideline standards of colorectal cancer screening, work flow of clinical and genetic activities, pathways of colorectal cancer screening and functionality of computer based guidelines and barriers. Electronic guideline standards of colorectal cancer screening were described in the s3 categories of content standard, telecommunications and technical standards and nomenclature and classification standards. According to the participations' views, workflow and genetic pathways of colorectal cancer screening were identified. Conclusions: The study demonstrated an effective role of computer-guided consultation for screening management. Electronic based systems facilitate real-time decision making during a clinical interaction. Electronic pathways have been applied for clinical and genetic decision support, workflow management, update recommendation and resource estimates. A suitable technical and clinical infrastructure is an integral part of clinical practice guidline of screening. As a conclusion, it is recommended to consider the necessity of architecture assessment and also integration standards.